Abstract：
:The aim of the present study was to explore the effect of baicalin on liver hypoxia/reoxygenation (H/R) injury and the possible mechanism involved. A cellular H/R model was established and cells were treated with 50, 100 and 200 µmol/l baicalin. Following reoxygenation for 6 h, cell viability, lactate dehydrogenase (LDH), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), caspase 3 and cleaved caspase 3 were assessed. Furthermore, levels of endoplasmic reticulum stress markers binding of immunoglobulin protein (BIP) and CCAAT/enhancer-binding protein homologous protein (CHOP) and autophagy markers microtubule-associated proteins 1A/1B light chain 3B (LC3) and beclin 1 were measured. To confirm the involvement of autophagy in baicalin-mediated attenuation of H/R injury, the autophagy inhibitor 3-methyladenine (3-MA) was administered. The results revealed that baicalin administration increased cell viability and decreased LDH levels, most notably at a dosage of 100 µmol/l. Baicalin pretreatment also downregulated the expression of caspase 3, cleaved caspase 3 and Bax, while upregulating the expression of Bcl-2. Furthermore, BIP and CHOP were decreased while LC3 and beclin-1 were significantly increased by baicalin pretreatment. Inhibiting autophagy using 3-MA, resulted in a significant decrease in LC3-II, beclin-1 and LDH, as well as increase in the expression of BIP, CHOP, caspase 3, cleaved caspase 3 and Bax. Bcl-2 and cell viability were also decreased. In conclusion, the results of the present study indicate that baicalin exerts a protective effect on liver H/R injury and this may be achieved via the induction of autophagy.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Liu F,Zhang J,Qian J,Wu G,Ma Zdoi
10.3892/etm.2018.6284subject
Has Abstractpub_date
2018-08-01 00:00:00pages
657-664issue
2eissn
1792-0981issn
1792-1015pii
ETM-0-0-6284journal_volume
16pub_type
杂志文章abstract：:A standard systemic therapy for patients with human epidermal growth factor receptor 2 (HER2)-negative advanced breast cancer (ABC) is yet to be identified. Sorafenib has been developed for the treatment of solid tumors, including breast cancer, as an oral multikinase inhibitor with antiangiogenic and antiproliferativ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2014.1603
更新日期：2014-05-01 00:00:00
abstract：:The effect of hepatitis B immune globulin (HBIG) combined with hepatitis B vaccine on blocking hepatitis B virus (HBV) transmission between mother and infant and its effect on immune cells were studied. Ninety newborn infants confirmed to be HBV surface antigen (HBsAg)-positive were divided equally into three groups. ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.5474
更新日期：2018-01-01 00:00:00
abstract：:Hepatitis B virus (HBV) can establish a lifelong chronic infection in humans, leading to liver cirrhosis, liver failure and hepatocellular carcinoma. Patients with chronic hepatitis B (CHB) exhibit a weak virus-specific immune response. Regulatory T cells (Tregs) play a key role in regulating the immune response in pa...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9107
更新日期：2020-10-01 00:00:00
abstract：:Systemic therapy in patients with concurrent psoriasis and chronic hepatitis B is a challenging task for both dermatologists and gastroenterologists since there is a high risk for hepatitis B virus (HBV) reactivation and hepatic toxicity under biological therapy. The therapeutic management of a patient with psoriasis ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2019.7567
更新日期：2019-08-01 00:00:00
abstract：:Cnidium officinale Makino and Capsella bursa-pastoris are used as traditional herbs with diverse medicinal effects, including the inhibition of inflammation, reduction of blood pressure and as diuretics, however, the anti-cancer effects of C. officinale Makino and C. bursa-pastoris are poorly defined. The aims of this...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2012.871
更新日期：2013-03-01 00:00:00
abstract：:The association between Helicobacter pylori (H. pylori) infection and metabolic syndrome (MetS) determined in different cohorts from different countries is currently inconclusive. In the majority of previous studies, H. pylori infection was diagnosed based on the presence of H. pylori IgG antibody in the serum. Howeve...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2019.7509
更新日期：2019-06-01 00:00:00
abstract：:The aim of this study was to investigate the effect of ouabain (EO) on myocardial remodeling. Twenty-two adult male Sprague-Dawley rats were randomly divided into two groups: the rats in the EO group (n=12) were intraperitoneally injected with EO daily and those in the control group (n=10) were injected with physiolog...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2013.1098
更新日期：2013-07-01 00:00:00
abstract：:Resveratrol (3, 4', 5-trihydroxy-trans-stilbene) is a natural, non-flavonoid polyphenol that exerts protective properties against atherosclerosis-associated endothelial dysfunction and senescence. The present study aimed to assess the influence of resveratrol on vascular contractility and molecular factors including s...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.4180
更新日期：2017-05-01 00:00:00
abstract：:Scarred uterus is caused by cesarean section surgery, and this condition affects further gestation and delivery in patients. Previous evidence suggested that resveratrol, a polyphenol compound, presents beneficial outcomes for patients with scarred uterus. Therefore, the aim of the present study was to analyze the eff...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.6126
更新日期：2018-06-01 00:00:00
abstract：:The clinical efficacy of minimally invasive lumbar interbody fusion via the intervertebral foramen combined with ozone (O3) therapy for the treatment of L3-L4 central-type lumbar disc herniation was explored. We recruited patients with sciatica who attended our hospital between July 2013 and October 2015 and underwent...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2016.4009
更新日期：2017-02-01 00:00:00
abstract：:Treatment of neonatal animals with capsaicin has previously been associated with long-lasting hyperthermia and severe cutaneous lesions. The present study analyzed the effects of capsaicin-induced hyperthermia on the occurrence of infectious disease and pruritic dermatitis in a rat model. Pregnant Sprague-Dawley (SD) ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2015.2829
更新日期：2015-12-01 00:00:00
abstract：:The aim of the present study was to determine the optimal intensity of anticoagulation therapy in elderly patients with paroxysmal atrial fibrillation (PAF), using aspirin and varied concentrations of warfarin. Elderly patients with PAF (n=1,162) who met the inclusion criteria of the study and were at middle or high-r...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2013.1141
更新日期：2013-08-01 00:00:00
abstract：:Cisplatin (DDP) is a commonly used chemotherapy drug; however, the side effects associated with its use, particularly acute kidney injury (AKI), limit its clinical application. Puerarin is a natural flavonoid extracted from the Chinese medical herb Radix puerariae, which has been reported to alleviate DDP-induced neph...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9081
更新日期：2020-10-01 00:00:00
abstract：:The present study evaluated the association between different Mycoplasma pneumoniae (M. pneumonia) genotypes and clinical features of pediatric patients. Subjects were children diagnosed with community-acquired pneumonia at the Children's Hospital of Soochow University (Suzhou, China) from January 2012 to December 201...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.4721
更新日期：2017-09-01 00:00:00
abstract：:Chronic venous disorder (CVD) is a complex disease, that affects millions of people worldwide, and due to the fact that in its early stages is often overlooked by healthcare providers and ignored by the patient, the assessment of incidence and prevalence of CVD is difficult to be made. The aim of this project was to a...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.7059
更新日期：2019-02-01 00:00:00
abstract：:Lung adenocarcinoma (LACA) is the leading cause of cancer-associated death worldwide. The present study intended to identify DNA methylation patterns that may serve as diagnostic and prognostic biomarkers for LACA. Data on DNA methylation and the survival data of the patients of LACA were obtained from The Cancer Geno...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2019.8200
更新日期：2020-01-01 00:00:00
abstract：:Parathyroid hormone-related protein (PTHrP) has been known to play an important role in the formation of meta-static lesions in the bone. However, there remains controversy over its practical role in predicting the occurrence of bone metastasis and the prognosis of breast cancer patients. In this study, we attempted t...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2012.521
更新日期：2012-06-01 00:00:00
abstract：:Toxoplasmosis is a zoonotic infection caused by the obligate intracellular apicomplexan parasite Toxoplasma gondii (T. gondii). T. gondii infection is a cause of congenital infection worldwide. Primary infection or the reactivation of latent infection during pregnancy may lead to fetal infection and to congenital toxo...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9012
更新日期：2020-10-01 00:00:00
abstract：:The present study aimed to investigate the effects of mechanical stretch and lipopolysaccharides (LPS) on the expression of transforming growth factor-β1 (TGF-β1) and collagen and viscoelasticity in human embryonic MRC-5 lung fibroblasts cultured in vitro and to assess the mechanisms of ARDS-associated ventilator-indu...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9133
更新日期：2020-11-01 00:00:00
abstract：:The present study aims to investigate the effects and underlying mechanisms of miRNA-145 (miR-145) in rat models of chronic constriction injury (CCI). Rats were randomly divided into control, sham, CCI, agomiRNA (agomiR)-normal control (NC) and agomiR-145 groups (n=25 in each group); in addition, 30 rats with CCI were...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2016.3900
更新日期：2016-12-01 00:00:00
abstract：:The curative effect of minimally invasive surgery on palmar and foot hyperhidrosis and its influence on serum-related cytokines and immunoglobulins were investigated. Seventy-six patients with palmar and foot hyperhidrosis admitted to Yunnan University Hospital from August 2014 to July 2016 were selected and randomly ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.5873
更新日期：2018-04-01 00:00:00
abstract：:The alleviation of brain injury is a key issue following cardiopulmonary resuscitation (CPR). Hydrogen sulfide (H2S) is hypothesized to be involved in the pathophysiological process of ischemia-reperfusion injury, and exerts a protective effect on neurons. The aim of the present study was to investigate the effects of...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2015.2950
更新日期：2016-02-01 00:00:00
abstract：:The aim of the present study was to provide reliable experimental evidence for the application of autologous skin fibroblasts (asFbs) in the repair of depressed scars. In the experiments, depressed trauma was induced in male Wistar rats, and fibroblasts were separated from the removed skin tissues to culture in medium...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2016.3442
更新日期：2016-08-01 00:00:00
abstract：:Uncommon causes of nail failures and surgical reinterventions were determined. The study included 23 osteoporotic patients, 13 of whom followed a fast recovery program with early walking (FWB group). The other 10 patients were not allowed full weight bearing until 6 weeks (NFWB group). The T-score was determined befor...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9346
更新日期：2020-12-01 00:00:00
abstract：:Endometriosis (EM) is a common benign gynecological disorder. The present study aimed to investigate the potential role of propofol, a commonly used intravenous anesthetic agent, in the pathogenesis of EM. The EM cell line CRL-7566 was used in the present study. CRL-7566 cells were first treated with various concentra...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.6648
更新日期：2018-10-01 00:00:00
abstract：:Melatonin is a darkness hormone secreted by the pineal gland, which serves a role in idiopathic oligoasthenoteratozoospermia (iOAT). The present study aimed to evaluate the seminal plasma and serum melatonin levels of 50 patients with iOAT and 50 normal fertile controls and the effects of exposure to light at night on...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.8678
更新日期：2020-07-01 00:00:00
abstract：:Effects of antithrombotic agents on the bleeding risk after transurethral resection of the prostate (TURP) were assessed in patients with benign prostatic hyperplasia (BPH). Controlled clinical trials on the effects of perioperative anticoagulant therapy on postoperative bleeding in BPH patients published during Janua...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9102
更新日期：2020-10-01 00:00:00
abstract：:In the present study, the predictive value of glycosylated hemoglobin (HbA1c), microalbuminuria (24 h mAlb) and serum cystatin C (Cys-C) levels on the outcome of pregnancy in patients with gestational diabetes mellitus (GDM) was investigated. Samples of 144 females with GDM and 117 normal pregnant females as controls ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2019.8336
更新日期：2020-02-01 00:00:00
abstract：:Monosialotetrahexosylganglioside sodium (GM1) is widely used in the treatment of central and peripheral neurological injuries. In addition to its neuroprotective activity, GM1 exerts protective effects on brain microvascular endothelial cells, although the mechanisms underlying these effects remain unclear. The aim of...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2015.2603
更新日期：2015-09-01 00:00:00
abstract：:The present study aimed to investigate the capabilities of the cardiovascular virtual endoscopy (VE) system in diagnosing tetralogy of Fallot (TOF) and performing measurements. A total of 37 patients underwent two-dimensional echocardiography (2-DE) and multi-detector computed tomography (MDCT) examinations. The obtai...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.5572
更新日期：2018-02-01 00:00:00
