Abstract:
:Recent whooping cough (pertussis) outbreaks in many countries highlight the crucial need for a better understanding of the pathogenesis of Bordetella pertussis infection of the respiratory tract. The baboon is a recently described preclinical model for the study of B. pertussis infection and may be ideal for the evaluation of new pertussis vaccines. However, many pathophysiological aspects, including bacterial localization and interactions, have yet to be described in this model. Here, we used a baboon model of infection with a fluorescent GFP-expressing B. pertussis strain, derived from European clinical isolate B1917. Juvenile baboons were used to evaluate susceptibility to infection and transmission. Non-invasive in vivo imaging procedures, using probe-based confocal endomicroscopy coupled with bronchoscopy, were developed to track fluorescent bacterial localization and cellular interactions with host cells in the lower respiratory tract of infected animals. All B1917-GFP-challenged animals developed classical pertussis symptoms, including paroxysmal cough, nasopharyngeal colonization, and leukocytosis. In vivo co-localization with antigen presenting cells and progressive bacterial colonization of the lower airways were also assessed by imaging during the first weeks of infection. Our results demonstrate that in vivo imaging can be used to assess bacterial colonization and to point out interactions in a baboon model of pertussis.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Naninck T,Coutte L,Mayet C,Contreras V,Locht C,Le Grand R,Chapon Cdoi
10.1038/s41598-018-30896-7subject
Has Abstractpub_date
2018-08-16 00:00:00pages
12297issue
1issn
2045-2322pii
10.1038/s41598-018-30896-7journal_volume
8pub_type
杂志文章abstract::Coccolithophores are single-celled photosynthesizing marine algae, responsible for half of the calcification in the surface ocean, and exert a strong influence on the distribution of carbon among global reservoirs, and thus Earth's climate. Calcification in the surface ocean decreases the buffering capacity of seawate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34263
更新日期:2016-09-28 00:00:00
abstract::Iron oxide nanoparticles (IONPs) are known to induce cytotoxicity in various cancer cell lines through the generation of reactive oxygen species (ROS). However, the studies on its potential to induce toxicity in normal cell lines and in vivo system are limited and ambiguity still exists. Additionally, small molecules ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43188-5
更新日期:2019-05-06 00:00:00
abstract::Zolpidem is not a typical GABAA receptor hypnotic. Unlike benzodiazepines, zolpidem modulates tonic GABA currents in the rat dorsal motor nucleus of the vagus, exhibits residual effects in mice lacking the benzodiazepine binding site, and improves speech, cognitive and motor function in human patients with severe brai...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28674
更新日期:2016-06-27 00:00:00
abstract::Skin allergy is a chronic condition that affects about 20% of the population of the western world. This disease is caused by small reactive compounds, haptens, able to penetrate into the epidermis and modify endogenous proteins, thereby triggering an immunogenic reaction. Phenyl isothiocyanate (PITC) and ethyl isothio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21203
更新日期:2016-02-17 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-23132-9
更新日期:2018-03-19 00:00:00
abstract::Thioflavin T (ThT) is standardly used as a fluorescent marker to detect aggregation of amyloid fibrils by conventional fluorescence microscopy, including polarization resolved imaging that brings information on the orientational order of the fibrils. These techniques are however diffraction limited and cannot provide ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12864-9
更新日期:2017-10-02 00:00:00
abstract::The excessive release and accumulation of glutamate in the brain is known to be associated with excitotoxicity. CE, an extract derived from the plant Coeloglossum viride var. Bracteatum, exerted neuroprotective effects against amyloid toxicity and oxidative stress in cortical neurons. The aims of this study are to exa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08957-0
更新日期:2017-08-23 00:00:00
abstract::Common estimates of external training intensity for resistance exercise do not incorporate inter-set recovery duration, and might not reflect the overall demands of training. This study aimed to assess novel metrics of exercise density (ED) during resistance exercise, and how these related to a physiological marker of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05953-2
更新日期:2017-07-17 00:00:00
abstract::Understanding the transcriptional regulatory elements that influence the progression of liver disease in the presence of hepatitis C virus (HCV) infection is critical for the development of diagnostic and therapeutic approaches. Systems biology provides a roadmap by which these elements may be integrated. In this stud...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32464-5
更新日期:2018-09-24 00:00:00
abstract::Bacterial artificial chromosomes (BACs) offer a means of manipulating gene expression and tagging gene products in the mammalian genome without the need to alter endogenous gene structure and risk deleterious phenotypic consequences. However, for a BAC clone to be useful for such purposes it must be shown to contain a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35458-5
更新日期:2018-11-23 00:00:00
abstract::Mycobacterium tuberculosis (M.tb), the pathogen responsible for tuberculosis (TB) poses as the major cause of death among infectious diseases. The knowledge about the molecular diversity of M.tb enables the implementation of more effective surveillance and control measures and, nowadays, Whole Genome Sequencing (WGS) ...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-020-69755-9
更新日期:2020-07-30 00:00:00
abstract::Incident myocardial infarction (MI) is a leading cause of adult mortality in the United States. However, because MI has a relatively low incidence in the young population, little information exists on the disease in younger adults. Because the retina has the unique quality that its vasculature is readily and noninvasi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24083-x
更新日期:2018-04-04 00:00:00
abstract::In 2010, a pathogenic flavivirus termed duck Tembusu virus (DTMUV) caused widespread outbreak of egg-drop syndrome in domesticated ducks in China. Although the glycoprotein E of DTMUV is an important structural component of the virus, the B-cell epitopes of this protein remains uncharacterized. Using phage display and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36288
更新日期:2016-11-08 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-53956-y
更新日期:2019-11-21 00:00:00
abstract::This study investigated Staphylococcus aureus carriage in patients with microbial keratitis (MK). 215 patients with MK, 60 healthy controls and 35 patients with rheumatoid arthritis (RA) were included. Corneal scrapes were collected from patients with MK. Conjunctival, nasal and throat swabs were collected from the no...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75821-z
更新日期:2020-10-29 00:00:00
abstract::Terminally misfolded proteins are selectively recognized and cleared by the endoplasmic reticulum-associated degradation (ERAD) pathway. SEL1L, a component of the ERAD machinery, plays an important role in selecting and transporting ERAD substrates for degradation. We have determined the crystal structure of the mouse...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20261
更新日期:2016-02-09 00:00:00
abstract::The introduced oriental shrimp Palaemon macrodactylus has recently become widespread in temperate estuaries worldwide. However, this recent worldwide spread outside of its native range arises after a previous introduction to the US Pacific coast, where it was restricted for more than 30 years. Using a phylogeographic ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05808
更新日期:2014-07-24 00:00:00
abstract::The present work focuses on the dynamical aspects of cross-reactivity between myelin based protein (MBP) self-peptide and two microbial peptides (UL15, PMM) for Hy.1B11 T-cell receptor (TCR). This same TCR was isolated from a patient suffering from multiple sclerosis (MS). The study aims at highlighting the chemical i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42496
更新日期:2017-02-14 00:00:00
abstract::CD74 is expressed on the cell surface of pulmonary macrophages and contributes to macrophage migration inhibitory factor (MIF)-induced inflammatory response in acute lung injury (ALI). A circulating form of CD74 (soluble CD74, sCD74) was recently discovered in autoimmune liver disease. Using two murine ALI models and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30067
更新日期:2016-07-22 00:00:00
abstract::Heterostructures based on two-dimensional (2D) materials have sparked wide interests in both fundamental physics and applied devices. Recently, Dirac/Weyl semimetals are emerging as capable functional materials for optoelectronic devices. However, thus far the interfacial coupling of an all-semimetal 2D heterostructur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29717-8
更新日期:2018-08-27 00:00:00
abstract::Clouds play a major role in the climate system, but large uncertainties remain about their decadal variations. Here we report a widespread decrease in cloud cover since the 1970 s over the Mediterranean region, in particular during the 1970 s-1980 s, especially in the central and eastern areas and during springtime. C...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41475
更新日期:2017-02-02 00:00:00
abstract::The use of O-(2-[18F]fluoroethyl)-L-tyrosine ([18F]FET) as a positron emission tomography (PET) tracer for brain tumor imaging might have some limitations because of the relatively low affinity for the L-type amino acid transporter 1 (LAT1). To assess the stereospecificity and evaluate the influence of aromatic ring m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40013-x
更新日期:2019-02-27 00:00:00
abstract::Time-series metabolomics studies can provide insight into the dynamics of disease development and facilitate the discovery of prospective biomarkers. To improve the performance of early risk identification, a new strategy for analyzing time-series data based on dynamic networks (ATSD-DN) in a systematic time dimension...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32448
更新日期:2016-08-31 00:00:00
abstract::A forest of the black coral Antipathella subpinnata was found from 52 to 80 m depth in three different sites at Tremiti Islands Marine Protected Area (MPA; Mediterranean Sea), with two of them hosting a monospecific forest on horizontal and vertical substrates. Colonies of A. subpinnata showed a mean density between 0...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65266-9
更新日期:2020-05-22 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-62901-3
更新日期:2020-04-20 00:00:00
abstract::The behavior of diatomic molecular solids under pressure have attracted great interest and been extensively studied. Under ambient pressure, the structure of bromine is known to be a molecular phase (phase I). With increasing pressure, it transforms into an incommensurate phase (phase V) before eventually to a monoato...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25649
更新日期:2016-05-09 00:00:00
abstract::Printable, wide band-gap chalcopyrite compound films (CuInGaS2, CIGS) were synthesized on transparent conducting oxide substrates. The wide band-gap and defective nature of the films reveal semi-transparent and bifacial properties that are beneficial for power generating window applications. Importantly, solar cell de...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04408
更新日期:2014-03-18 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-22649-3
更新日期:2018-03-12 00:00:00
abstract::Fatty acid oxidation and subsequent ketogenesis is one of the major mechanisms to maintain hepatic lipid homeostasis under fasting conditions. Fasting hormone glucagon has been shown to stimulate ketone body production through activation of PPARα; however, the signal pathway linking glucagon to PPARα is largely undisc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23317
更新日期:2016-03-17 00:00:00
abstract::Modulating the tumor microenvironment to promote an effective immune response is critical in managing any type of tumor. Melanoma is an aggressive skin cancer and the incidence rate is increasing worldwide. Potent protease inhibitors have recently been extensively researched as potential therapeutic agents against var...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52609-4
更新日期:2019-11-07 00:00:00