Abstract:
:Brain‑type glycogen phosphorylase (PYGB) is an enzyme that metabolizes glycogen, whose function is to provide energy for an organism in an emergency state. The present study purposed to investigate the role and mechanism of PYGB silencing on the growth and apoptosis of prostate cancer cells. A cell counting kit‑8 assay and flow cytometry were performed to determine the cell viability, apoptosis and reactive oxygen species (ROS) content, respectively. Colorimetry was performed to analyze the activity of caspase‑3. Western blotting and reverse transcription‑quantitative polymerase chain reaction were used to evaluate the associated mRNA and protein expression levels. The results revealed that PYGB was upregulated in prostate cancer tissues and was associated with disease progression. In addition, PYGB silencing suppressed the cell viability of PC3 cells. PYGB silencing promoted apoptosis of PC3 cells via the regulation of the expression levels of cleaved‑poly (adenosine diphosphate‑ribose) polymerase, cleaved‑caspase‑3, B‑cell lymphoma‑2 (Bcl‑2) and Bcl‑2‑associated X protein. PYGB silencing increased the ROS content in PC3 cells, and affected nuclear factor (NF)‑κB/nuclear factor‑erythroid 2‑related factor 2 (Nrf2) signaling pathways in PC3 cells. In conclusion, PYGB silencing suppressed the growth and promoted the apoptosis of prostate cancer cells by affecting the NF‑κB/Nrf2 signaling pathway. The present study provided evidence that may lead to the development of a potential therapeutic strategy for prostate cancer.
journal_name
Mol Med Repjournal_title
Molecular medicine reportsauthors
Wang Z,Han G,Liu Q,Zhang W,Wang Jdoi
10.3892/mmr.2018.9388subject
Has Abstractpub_date
2018-10-01 00:00:00pages
3800-3808issue
4eissn
1791-2997issn
1791-3004journal_volume
18pub_type
杂志文章abstract::Accumulating evidence has revealed that the mammalian heart possesses a measurable capacity for renewal. Neonatal mice retain a regenerative capacity over a short time-frame (≤6 days), but this capacity is lost by 7 days of age. In the present study, differential gene expression profiling of mouse cardiac tissue was p...
journal_title:Molecular medicine reports
pub_type: 杂志文章
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.4700
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2013.1716
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.4539
更新日期:2016-01-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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更新日期:2017-07-01 00:00:00
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journal_title:Molecular medicine reports
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journal_title:Molecular medicine reports
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journal_title:Molecular medicine reports
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journal_title:Molecular medicine reports
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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journal_title:Molecular medicine reports
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00