Nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging genome or polyelectrolyte.

Abstract:

:The survival of viruses partly relies on their ability to self-assemble inside host cells. Although coarse-grained simulations have identified different pathways leading to assembled virions from their components, experimental evidence is severely lacking. Here, we use time-resolved small-angle X-ray scattering to uncover the nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging their full RNA genome. We reveal the formation of amorphous complexes via an en masse pathway and their relaxation into virions via a synchronous pathway. The binding energy of capsid subunits on the genome is moderate (~7kBT0, with kB the Boltzmann constant and T0 = 298 K, the room temperature), while the energy barrier separating the complexes and the virions is high (~ 20kBT0). A synthetic polyelectrolyte can lower this barrier so that filled capsids are formed in conditions where virions cannot build up. We propose a representation of the dynamics on a free energy landscape.

journal_name

Nat Commun

journal_title

Nature communications

authors

Chevreuil M,Law-Hine D,Chen J,Bressanelli S,Combet S,Constantin D,Degrouard J,Möller J,Zeghal M,Tresset G

doi

10.1038/s41467-018-05426-8

subject

Has Abstract

pub_date

2018-08-06 00:00:00

pages

3071

issue

1

issn

2041-1723

pii

10.1038/s41467-018-05426-8

journal_volume

9

pub_type

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