High levels of circulating cell-free DNA are a biomarker of active SLE.

Abstract:

BACKGROUND:High levels of circulating cell-free DNA (cfDNA) have been reported in patients with inflammatory conditions. The aim of the study was to investigate the levels of cfDNA in patients with systemic lupus erythematosus (SLE). MATERIALS AND METHODS:Comparative groups comprised 22 nonpregnant and 36 pregnant women with SLE (test groups) and 60 nonpregnant and 199 pregnant women with no history of SLE (control groups). The levels of cfDNA in plasma were quantitated by a fluorometric dsDNA assay. RESULTS:Compared to controls, the median levels of cfDNA were significantly higher in nonpregnant SLE patients (7.38 ng/mL vs 4.6 ng/mL, P = 0.033) and in pregnant SLE patients (7.65 ng/mL vs 5.25 ng/mL, P = 0.003). Based on SLE disease activity index (SLEDAI) scores, the median cfDNA levels were significantly higher in patients with active disease (4 < SLEDAI < 15) compared with patients with inactive disease (SLEDAI < 4) (13.58 ng/mL vs 6.72 ng/mL, P = 0.01). While there was a trend of increased cfDNA levels with higher SLEDAI scores (R2  = 0.3, P < 0.001), we found no association of increased cfDNA levels with nephritis, skin manifestations, multiorgan inflammations or with other inflammatory markers such as decreased C3 and C4 levels or increased anti-ds DNA antibodies. CONCLUSIONS:Our results suggest that in addition to classical SLE serological markers, measurement of circulating plasma cfDNA levels has potential as a useful biomarker for assessing SLE disease activity in patients and monitoring treatment.

journal_name

Eur J Clin Invest

authors

Xu Y,Song Y,Chang J,Zhou X,Qi Q,Tian X,Li M,Zeng X,Xu M,Zhang W,Cram DS,Liu J

doi

10.1111/eci.13015

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

e13015

issue

11

eissn

0014-2972

issn

1365-2362

journal_volume

48

pub_type

杂志文章
  • Polymorphonuclear leucocyte dysregulation in patients with gram-negative septicaemia assessed by flow cytometry.

    abstract::Flow cytometry was used to study phagocytic function and release of reactive oxygen intermediates (ROI) following phagocytosis by granulocytes in 14 patients (six female, eight male) with gram-negative septicaemia prior to, during, and after therapy compared with a group of healthy controls. Phagocytic capacity was as...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.1995.tb01723.x

    authors: Wenisch C,Parschalk P,Hasenhündl M,Griesmacher A,Graninger W

    更新日期:1995-06-01 00:00:00

  • Renal clearance of atrial natriuretic peptide during acute unilateral complete ureteral obstruction in the pig.

    abstract::Renal extraction and renal plasma clearance of atrial natriuretic peptide from pigs with complete unilateral ureteral obstruction (UUO) and from intact anaesthetized pigs were determined from arteriovenous differences in plasma atrial natriuretic peptide and measured renal plasma flow. The effect of administration of ...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.1995.tb01556.x

    authors: Frøkiaer J,Djurhuus JC,Pedersen EB

    更新日期:1995-04-01 00:00:00

  • In vitro immune modulation of polymorphonuclear leukocyte adhesiveness by sodium fluoride.

    abstract::We investigated the influence of sodium fluoride on polymorphonuclear leukocyte (PMN) adhesiveness in a healthy subject with low serum levels of fluoride. The PMN were separated from venous blood, and the percentages of adhered and unadhered cells were determined in vitro in plastic culture plates. The cells were cult...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.1992.tb01426.x

    authors: Gómez-Ubric JL,Liébana J,Gutiérrez J,Castillo A

    更新日期:1992-10-01 00:00:00

  • The association between CYBA gene C242T variant and risk of metabolic syndrome.

    abstract:BACKGROUND:Both inflammation and oxidative stress may contribute to pathogenesis of metabolic syndrome (MetS). The C242T polymorphism (rs4673) in the CYBA gene, as the main components of NAD (P) H oxidase, causes inter-individual variability in the enzyme activity. We aimed to investigate the association between this p...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/eci.13275

    authors: Pourgholi L,Pourgholi F,Ziaee S,Goodarzynejad H,Hosseindokht M,Boroumand M,Mandegary A

    更新日期:2020-09-01 00:00:00

  • The influence of sorafenib on hepatic encephalopathy and the mechanistic survey in cirrhotic rats.

    abstract:BACKGROUND:Sorafenib, a multikinase inhibitor that inhibits angiogenesis and carcinogenesis, has been used for patients with advanced hepatocellular carcinoma. However, sporadic cases have been reported with the development of hepatic encephalopathy (HE) after sorafenib treatment, mostly in those with cirrhosis. Liver ...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/eci.12006

    authors: Hsu SJ,Hsin IF,Lin YL,Chen YC,Huang HC,Lee FY,Lin HC,Chang CC,Lee SD

    更新日期:2012-12-01 00:00:00

  • Pravastatin for thioacetamide-induced hepatic failure and encephalopathy.

    abstract:BACKGROUND:Nitric oxide (NO) inhibition aggravates hepatic damage and encephalopathy and increases mortality in rats with thioacetamide (TAA)-induced acute liver failure. Statins enhance NO production but whether they influence the above parameters are unknown. MATERIAL AND METHODS:Male Sprague-Dawley rats were used. ...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.2011.02566.x

    authors: Huang HC,Chang CC,Wang SS,Chan CY,Lee FY,Chuang CL,Hsin IF,Teng TH,Lin HC,Lee SD

    更新日期:2012-02-01 00:00:00

  • Angiotensin receptor blockers are associated with a lower remodelling score of stenotic aortic valves.

    abstract:BACKGROUND:Experimental and clinical studies have suggested that inhibitors of the renin angiotensin system (RAS) might be useful to slow the progression of valvular calcification in patients with aortic stenosis (AS). OBJECTIVES:The aim of this study was to evaluate the relationships between the weight and tissue rem...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.2011.02522.x

    authors: Côté N,Couture C,Pibarot P,Després JP,Mathieu P

    更新日期:2011-11-01 00:00:00

  • Immunologic and viral markers in the circulation of anti-HIV negative heroin addicts.

    abstract::To assess the degree of immune system activation associated with addiction or hepatotropic viruses infection, we examined 60 HIV-negative heroin addicts for the presence of hepatitis B virus (HBV) infection markers, hepatitis C virus antibodies (anti-HCV), various auto-antibodies, and serum levels of soluble interleuk...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.1993.tb00765.x

    authors: Dalekos GN,Manoussakis MN,Zervou E,Tsianos EV,Moutsopoulos HM

    更新日期:1993-04-01 00:00:00

  • Bacteria and spontaneous experimental colitis: immunological changes.

    abstract:BACKGROUND:Intestinal commensal flora seems to be a requisite for both human and experimental intestinal inflammation. Our aim was to assess the immunological changes in the colon of IL-10(-/-) mice depending on the environmental conditions. MATERIALS AND METHODS:Twelve wild-type (WT) and 24 IL-10(-/-) 4-week-old mice...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.2011.02499.x

    authors: Pedrosa E,Lorén V,Cabré E,Doménech E,Ojanguren I,Gassull MA,Mañé J

    更新日期:2011-10-01 00:00:00

  • Elevated cell-associated levels of interleukin 1beta and interleukin 6 in inflamed mucosa of inflammatory bowel disease.

    abstract::The aim of this study was to investigate the involvement of the monocyte-derived cytokines interleukin 1beta (IL-1beta), interleukin 6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) in idiopathic inflammatory bowel disease. Endoscopic biopsies of normal and inflamed intestinal mucosa were obtained from patients w...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1046/j.1365-2362.1996.123262.x

    authors: Grottrup-Wolfers E,Moeller J,Karbach U,Muller-Lissner S,Endres S

    更新日期:1996-02-01 00:00:00

  • Altered intestinal tight junctions' expression in patients with liver cirrhosis: a pathogenetic mechanism of intestinal hyperpermeability.

    abstract:BACKGROUND:Increased intestinal permeability in cirrhosis exerts a pivotal role in the pathogenesis of spontaneous bacterial peritonitis and other complications of cirrhosis through promotion of systemic endotoxemia. This study was designed to investigate whether the expression of tight junction (TJ) proteins, which re...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.2011.02609.x

    authors: Assimakopoulos SF,Tsamandas AC,Tsiaoussis GI,Karatza E,Triantos C,Vagianos CE,Spiliopoulou I,Kaltezioti V,Charonis A,Nikolopoulou VN,Scopa CD,Thomopoulos KC

    更新日期:2012-04-01 00:00:00

  • Total serum haemolytic complement activity, erythrocyte sedimentation rate and plasma fibrinogen as indicators of the stage in Hodgkin's disease.

    abstract::Total serum haemolytic complement activity, plasma fibrinogen, erythrocyte sedimentation rate and other biological values in forty-three patients with Hodgkin's disease were correlated with results of staging. A highly significant increase (P=10(-5)) of the mean total serum haemolytic complement activity was found in ...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.1977.tb01607.x

    authors: Wagener DJ,Haanen C

    更新日期:1977-08-01 00:00:00

  • Cardiovascular response to lower body negative pressure stimulation before, during, and after space flight.

    abstract:BACKGROUND:It is well known that space travel cause post-flight orthostatic hypotension and it was assumed that autonomic cardiovascular control deteriorates in space. Lower body negative pressure (LBNP) was used to assess autonomic function of the cardiovascular system. METHODS:LBNP tests were performed on six crew-m...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1046/j.1365-2362.2000.00750.x

    authors: Baisch F,Beck L,Blomqvist G,Wolfram G,Drescher J,Rome JL,Drummer C

    更新日期:2000-12-01 00:00:00

  • Randomized placebo controlled trial of furosemide on subjective perception of dyspnoea in patients with pulmonary oedema because of hypertensive crisis.

    abstract:BACKGROUND:To compare the administration of furosemide with placebo on the subjective perception of dyspnoea in patients with acute pulmonary oedema because of hypertensive crisis. Design  Randomized, controlled and double-blinded clinical trial. SETTING:Municipal emergency medical service system and university-based ...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1365-2362.2010.02450.x

    authors: Holzer-Richling N,Holzer M,Herkner H,Riedmüller E,Havel C,Kaff A,Malzer R,Schreiber W

    更新日期:2011-06-01 00:00:00

  • Density and agonist-promoted high and low affinity states of the beta-adrenoceptor on human B- and T-cells.

    abstract::beta-Adrenoceptor binding on peripheral blood mononuclear cells (PBMC) of healthy adult volunteers was investigated using the radioligand 125iodo-cyanopindolol (ICYP). Saturation binding studies were performed with nine different concentrations of ICYP. Receptor density and affinity were calculated by Scatchard plots....

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.1988.tb02416.x

    authors: Griese M,Körholz U,Körholz D,Seeger K,Wahn V,Reinhardt D

    更新日期:1988-04-01 00:00:00

  • Molecular insights into insulin action and secretion.

    abstract::Tightly co-ordinated control of both insulin action and secretion is required in order to maintain glucose homeostasis. Gene knockout experiments have helped to define key signalling molecules that affect insulin action, including insulin and insulin-like growth factor-1 (IGF-1) receptors, insulin receptor substrate (...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章,评审

    doi:10.1046/j.1365-2362.32.s3.2.x

    authors: Rhodes CJ,White MF

    更新日期:2002-06-01 00:00:00

  • Gallbladder motility and cholesterol crystallization in bile from patients with pigment and cholesterol gallstones.

    abstract:BACKGROUND:Little is known about gallbladder motility in patients with black pigment stones when compared to cholesterol gallstone patients, or about their relationship to biliary composition, crystallization and stone characteristics. DESIGN:Fasting and postprandial gallbladder volumes were studied by ultrasonography...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1046/j.1365-2362.2000.00639.x

    authors: Portincasa P,Di Ciaula A,Vendemiale G,Palmieri V,Moschetta A,Vanberge-Henegouwen GP,Palasciano G

    更新日期:2000-04-01 00:00:00

  • Iron overload in myelodysplastic syndromes (MDS) - diagnosis, management, and response criteria: a proposal of the Austrian MDS platform.

    abstract::Transfusion-related morbidity is an emerging challenge in chronically transfused patients with low-risk myelodysplastic syndromes (MDS). In these patients, transfusion-induced iron overload may represent a leading medical problem. However, although iron-chelating drugs are available, little is known about optimal diag...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.2007.01915.x

    authors: Valent P,Krieger O,Stauder R,Wimazal F,Nösslinger T,Sperr WR,Sill H,Bettelheim P,Pfeilstöcker M

    更新日期:2008-03-01 00:00:00

  • Multiple biochemical effects in the pathogenesis of alcoholic fatty liver.

    abstract::The pathogenesis of alcoholic fatty liver is unknown, but several causes have been proposed based on biochemical findings. These include the metabolism of alcohol leading to a shift in the cytosolic [NAD+]/ [NADH] ratio to reduction, which in turn causes a direct inhibition of beta-oxidation and enhanced triacylglycer...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章,评审

    doi:10.1046/j.1365-2362.1997.1780727.x

    authors: Eaton S,Record CO,Bartlett K

    更新日期:1997-09-01 00:00:00

  • Relative down-regulation of apoptosis and autophagy genes in colorectal cancer.

    abstract:BACKGROUND:Cancer is often caused by disturbance in the regulation and/or execution of programmed cell death (PCD, including apoptosis and autophagy). Our aim was to investigate these two pathways simultaneously in the same samples to understand further the pathological roles of PCDs in colorectal cancer. MATERIALS AN...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.2010.02383.x

    authors: Chang YT,Tseng HC,Huang CC,Chen YP,Chiang HC,Chou FP

    更新日期:2011-01-01 00:00:00

  • Increased rate of lumbar spondylolisthesis in rheumatoid arthritis: A case-control study.

    abstract:OBJECTIVE:The aim of this study was to compare the prevalence of lumbar spondylolisthesis (SPL) in patients suffering from low back pain (LBP) with or without rheumatoid arthritis (RA) and to identify potential factors associated with SPL in patients with RA. METHODS:We performed a case-control study. Cases and contro...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/eci.12991

    authors: Hagege B,Tubach F,Alfaiate T,Forien M,Dieudé P,Ottaviani S

    更新日期:2018-09-01 00:00:00

  • Affinity of LDL to a human arterial proteoglycan among male survivors of myocardial infarction.

    abstract::In the present study, the hypothesis that the affinity of LDL to arterial proteoglycans might discriminate myocardial infarction patients from controls were tested. The patients were 52 men who had sustained a myocardial infarction at an age of 50 years or less and the controls, selected from a random population sampl...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.1989.tb00193.x

    authors: Linden T,Bondjers G,Camejo G,Bergstrand R,Wilhelmsen L,Wiklund O

    更新日期:1989-02-01 00:00:00

  • Factors influencing spontaneous mobilization of CD34+ and CD133+ progenitor cells after myocardial infarction.

    abstract:BACKGROUND:Bone marrow-derived circulating progenitor cells (BM-CPCs) are mobilized into adult peripheral blood (PB) during acute myocardial infarction (AMI) and may contribute to the regeneration of infarcted myocardium. The purpose of the present study is to determine whether mobilization of BM-CPCs into PB depends o...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.2007.01876.x

    authors: Turan RG,Brehm M,Koestering M,Tobias Z,Bartsch T,Steiner S,Picard F,Ebner P,Schannwell CM,Strauer BE

    更新日期:2007-11-01 00:00:00

  • Trans- and paracellular calcium transport across the colonic mucosa after short- and long-term treatment with 1,25-dihydroxyvitamin D3.

    abstract::The electrical parameters and the unidirectional fluxes of 45Ca and 3H-mannitol were measured in preparations of rat colon descendens freed from the muscularis externa and mounted in a modified Ussing-chamber. Two criteria were used to differentiate between changes in the trans- and the paracellular calcium transport ...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.1986.tb01007.x

    authors: Karbach U,Rummel W

    更新日期:1986-10-01 00:00:00

  • Uricaemia and ejection fraction in elderly heart failure outpatients.

    abstract:BACKGROUND:Preliminary data suggest that serum uric acid (SUA) could be involved in the prognosis of chronic heart failure (HF). The aim of our study was to test the relationship between SUA and left ventricular ejection fraction (EF%) in a cohort of elderly hypertensive outpatients with chronic HF. DESIGN:We consecut...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/eci.12273

    authors: Borghi C,Cosentino ER,Rinaldi ER,Cicero AF

    更新日期:2014-06-01 00:00:00

  • Pioglitazone shift circadian rhythm of blood pressure from non-dipper to dipper type in type 2 diabetes mellitus.

    abstract:BACKGROUND:Insulin resistance significantly correlated with a non-dipper type of essential hypertension. Thiazolidinediones (TZD), oral hypoglycaemic agents that act as insulin sensitizers, have been demonstrated in multiple in vivo and in vitro studies to possess antihypertensive properties. This study examined the ef...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.2007.01854.x

    authors: Anan F,Masaki T,Fukunaga N,Teshima Y,Iwao T,Kaneda K,Umeno Y,Okada K,Wakasugi K,Yonemochi H,Eshima N,Saikawa T,Yoshimatsu H

    更新日期:2007-09-01 00:00:00

  • Role of polyamines in intestinal adaptation in the rat.

    abstract::The cellular mechanisms controlling adaptive intestinal mucosal hypo- and hyperplasia are poorly understood but changes in tissue polyamine levels and in the activity of the key enzymes controlling their synthesis (ornithine decarboxylase: ODC) and degradation (diamine oxidase: DAO) have been implicated. Therefore, in...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.1987.tb01130.x

    authors: Hosomi M,Stace NH,Lirussi F,Smith SM,Murphy GM,Dowling RH

    更新日期:1987-10-01 00:00:00

  • Risk score to predict mortality in continuous ambulatory peritoneal dialysis patients.

    abstract:BACKGROUND:Patients with continuous ambulatory peritoneal dialysis (CAPD) have high all-cause mortality risk that varies extensively among different conditions. The objective of this study was to develop and validate risk models to predict the 2-year all-cause mortality risks of CAPD patients. MATERIAL AND METHODS:A t...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/eci.12344

    authors: Zhao C,Luo Q,Xia X,He F,Peng F,Yu X,Huang F

    更新日期:2014-11-01 00:00:00

  • TGF-beta1 overexpression in the transversalis fascia of patients with direct inguinal hernia.

    abstract:BACKGROUND:The aetiology of inguinal hernia includes changes in collagen turnover and metalloproteinase (MMP) expression, and direct hernia has been linked to increased MMP-2 expression. Since transforming growth factor beta1 (TGFbeta1) plays a role in tissue remodelling, this growth factor could directly affect metall...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.2007.01816.x

    authors: Pascual G,Corrales C,Gómez-Gil V,Buján J,Bellón JM

    更新日期:2007-06-01 00:00:00

  • Development of bone mineral loss in insulin-treated diabetes: a 1 1/2 years follow-up study in sixty patients.

    abstract::The change in bone mass during 1 1/2 years was determined in a longitudinal study of sixty adult insulin-treated diabetic out-patients. During the study period the mean bone mass decreased by 1.30 +/- 0.28 (SEM)% (P less than 0.001), to a mean value of 91.0 +/- 1.7% of normal (P less than 0.001). The rate of bone loss...

    journal_title:European journal of clinical investigation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2362.1981.tb01765.x

    authors: McNair P,Christiansen C,Christensen MS,Madsbad S,Faber OK,Binder C,Transbøl I

    更新日期:1981-02-01 00:00:00