Abstract：
:Hepcidin-25 was identified as the main iron regulator in the human body, and it by binds to the sole iron-exporter ferroportin. Studies showed that the N-terminus of hepcidin is responsible for this interaction, the same N-terminus that encompasses a small copper(II)-binding site known as the ATCUN (amino-terminal Cu(II)- and Ni(II)-binding) motif. Interestingly, this copper-binding property is largely ignored in most papers dealing with hepcidin-25. In this context, detailed investigations of the complex formed between hepcidin-25 and copper could reveal insight into its biological role. The present work focuses on metal-bound hepcidin-25 that can be considered the biologically active form. The first part is devoted to the reversed-phase chromatographic separation of copper-bound and copper-free hepcidin-25 achieved by applying basic mobile phases containing 0.1% ammonia. Further, mass spectrometry (tandem mass spectrometry (MS/MS), high-resolution mass spectrometry (HRMS)) and nuclear magnetic resonance (NMR) spectroscopy were employed to characterize the copper-peptide. Lastly, a three-dimensional (3D) model of hepcidin-25 with bound copper(II) is presented. The identification of metal complexes and potential isoforms and isomers, from which the latter usually are left undetected by mass spectrometry, led to the conclusion that complementary analytical methods are needed to characterize a peptide calibrant or reference material comprehensively. Quantitative nuclear magnetic resonance (qNMR), inductively-coupled plasma mass spectrometry (ICP-MS), ion-mobility spectrometry (IMS) and chiral amino acid analysis (AAA) should be considered among others.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Abbas IM,Vranic M,Hoffmann H,El-Khatib AH,Montes-Bayón M,Möller HM,Weller MGdoi
10.3390/ijms19082271subject
Has Abstractpub_date
2018-08-02 00:00:00issue
8issn
1422-0067pii
ijms19082271journal_volume
19pub_type
杂志文章abstract：:The plant hormone auxin plays pivotal roles in many aspects of plant growth and development. The auxin/indole-3-acetic acid (Aux/IAA) gene family encodes short-lived nuclear proteins acting on auxin perception and signaling, but the evolutionary history of this gene family remains to be elucidated. In this study, the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18102107
更新日期：2017-10-08 00:00:00
abstract：:Glutathione Transferases (GSTs) are phase II detoxification enzymes known to be involved in the molecular response against microcystins (MCs) induced toxicity. However, the individual role of the several GST isoforms in the MC detoxification process is still unknown. In this study, the time-dependent changes on gene e...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16048397
更新日期：2015-04-15 00:00:00
abstract：:Leaf senescence is an important agronomic trait that affects both crop yield and quality. In this study, we characterized a premature leaf senescence mutant of wheat (Triticum aestivum L.) obtained by ethylmethane sulfonate (EMS) mutagenesis, named m68. Genetic analysis showed that the leaf senescence phenotype of m68...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19030782
更新日期：2018-03-10 00:00:00
abstract：BACKGROUND:P1G10 is a cysteine proteolytic fraction from Vasconcellea cundinamarcensis latex, obtained by chromatographic separation on Sephadex-G10 and ultrafiltration. This fraction enhances healing in different models of skin lesions, and displays a protective/healing effect against gastric ulcers, where it was sugg...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184373
更新日期：2019-09-06 00:00:00
abstract：:Antrodan, a unique protein-bound polysaccharide derived from the fungal mycelia of Antrodia cinnamomea, has been reported to exhibit antitumor and anti-metastatic effects on Lewis lung carcinoma (LLC) cells through direct action and immunomodulation in vitro. In this study, we investigated the combined treatment of an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19061565
更新日期：2018-05-24 00:00:00
abstract：:Ovarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of MT2 in bovine GCs, and assess whether MT2 silencing affected GCs response to melatonin. We found that MT2 silencing significantly dec...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19072028
更新日期：2018-07-12 00:00:00
abstract：:The floral development in an important legume crop yellow lupine (Lupinus luteus L., Taper cv.) is often affected by the abscission of flowers leading to significant economic losses. Small non-coding RNAs (sncRNAs), which have a proven effect on almost all developmental processes in other plants, might be of key playe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205122
更新日期：2019-10-16 00:00:00
abstract：:KCNK10, a member of tandem pore domain potassium channel family, gives rise to leak K+ currents. It plays important roles in stabilizing the negative resting membrane potential and in counterbalancing depolarization. We previously demonstrated that kcnk10 expression is quickly elevated during the early stage of adipog...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms151222743
更新日期：2014-12-09 00:00:00
abstract：:MicroRNAs have been identified as key regulators of gene expression and thus their potential in disease diagnostics, prognosis and therapy is being actively pursued. Deregulation of microRNAs in cerebral pathogenesis has been reported to a limited extent in both animal models and human. Due to the complexity of the pa...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15011418
更新日期：2014-01-20 00:00:00
abstract：:Our laboratory and others have previously shown that cannabinoid receptor type-1 (CB1r) activity is neuroprotective and a modulator of brain ageing; a genetic disruption of CB1r signaling accelerates brain ageing, whereas the pharmacological stimulation of CB1r activity had the opposite effect. In this study, we have ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22010005
更新日期：2020-12-22 00:00:00
abstract：:Pterostilbene (PTE) is a natural sterbenoid contained in blueberries that has an antioxidant effect. In contrast, PTE also generates oxidative stress in cancer cells and provides an antitumor effect. Here, we examined the potential mechanism of this contrasting effect of PTE using three gastrointestinal cancer cell li...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249347
更新日期：2020-12-08 00:00:00
abstract：:Modern strategies in adipose tissue engineering (ATE) take advantage of the easy harvest, abundance and differentiation potential towards mesenchymal lineages of hADSCs. The controlled conversion of hADSCs to committed adipogenic precursors and further mature adipocytes formation is important for good long-term result...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms131215881
更新日期：2012-11-27 00:00:00
abstract：:The development of neural circuits is a complex process that relies on the proper navigation of axons through their environment to their appropriate targets. While axon-environment and axon-target interactions have long been known as essential for circuit formation, communication between axons themselves has only more...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21145170
更新日期：2020-07-21 00:00:00
abstract：:The ghrelin system has received substantial recognition as a potential target for novel anti-seizure drugs. Ghrelin receptor (ghrelin-R) signaling is complex, involving Gαq/11, Gαi/o, Gα12/13, and β-arrestin pathways. In this study, we aimed to deepen our understanding regarding signaling pathways downstream the ghrel...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20102480
更新日期：2019-05-20 00:00:00
abstract：:Lupus nephritis (LN) is one of the most common and severe complications of lupus. However, the mechanisms for renal damage have not been well elucidated. There are evidences show that glomerular endothelial cells (GECs) are damaged in LN. Immune complexes can deposit in subendothelial area and could affect GEC functio...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20215281
更新日期：2019-10-24 00:00:00
abstract：:Cisplatin is one of the most widely used chemical drugs for anticancer treatment. Recent studies have focused on the ability of cisplatin to retain the high mobility group box 1 (HMGB1) protein in cisplatin-DNA adducts, thereby preventing its release from the nucleus. Because HMGB1 is a powerful inflammatory mediator ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140611224
更新日期：2013-05-27 00:00:00
abstract：:Derivatives of 3,4-hydroxypyridinones have been extensively studied for in vivo Fe3+ sequestration. Deferiprone, a 1,2-dimethyl-3,4-hydroxypyridinone, is now routinely used for clinical treatment of iron overload disease. Hexadentate tris(3,4-hydroxypyridinone) ligands (THP) complex Fe3+ at very low iron concentration...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18010116
更新日期：2017-01-08 00:00:00
abstract：:Small noncoding RNAs that are 19-23 nucleotides long, known as microRNAs (miRNAs), are involved in almost all biological mechanisms during carcinogenesis. Recent studies show that miRNAs released from live cells are detectable in body fluids and may be taken up by other cells to confer cell-cell communication. These r...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms140714785
更新日期：2013-07-16 00:00:00
abstract：:Biologically active chemical compounds may provide remedies for several diseases. Meanwhile, Machine Learning techniques applied to Drug Discovery, which are cheaper and faster than wet-lab experiments, have the capability to more effectively identify molecules with the expected pharmacological activity. Therefore, it...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20092175
更新日期：2019-05-02 00:00:00
abstract：:Dry fruits consist of two types, dehiscent and indehiscent, whereby the fruit is splitting open or remains closed at maturity, respectively. The seed, the dispersal unit (DU) of dehiscent fruits, is composed of three major parts, the embryo and the food reserve, encapsulated by the maternally-derived organ, the seed c...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21218024
更新日期：2020-10-28 00:00:00
abstract：:InPBi exhibits broad and strong photoluminescence at room temperature, and is a potential candidate for fabricating super-luminescence diodes applied in optical coherence tomography. In this paper, the strained InPBi quantum dot (QD) embedded in the AlGaAs barrier on a GaAs platform is proposed to enhance the light em...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20236001
更新日期：2019-11-28 00:00:00
abstract：:The cytostatic agent hydroxyurea (HU) has proven to be beneficial for a variety of conditions in the disciplines of oncology, hematology, infectious disease and dermatology. It disrupts the S phase of the cell cycle by inhibiting the ribonucleotide reductase enzyme, thus blocking the transformation of ribonucleotides ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19123986
更新日期：2018-12-11 00:00:00
abstract：:Liquid water is considered to be a peculiar example of glass forming materials because of the possibility of giving rise to amorphous phases with different densities and of the thermodynamic anomalies that characterize its supercooled liquid phase. In the present work, literature data on the density of bulk liquid wat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197269
更新日期：2020-10-01 00:00:00
abstract：:The dysfunction of astrocytic inwardly rectifying potassium (Kir) 4.1 channels, which mediate the spatial potassium-buffering function of astrocytes, is known to be involved in the development of epilepsy. Here, we analyzed the Kir4.1 expressional changes in Leucine-Rich Glioma-Inactivated 1 (Lgi1) mutant rats, which ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051013
更新日期：2019-02-26 00:00:00
abstract：:(1) Background: A high incidence of intervening sequence (IVS)4+919 G>A mutation with later-onset cardiac phenotype have been reported in a majority of Taiwan Fabry cohorts. Some evidence indicated that conventional biomarkers failed to predict the long-term progression and therapeutic outcome; (2) Methods: In this st...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19051480
更新日期：2018-05-16 00:00:00
abstract：:Milk fat is a key factor affecting milk quality and is also a major trait targeted in dairy cow breeding. To determine how the synthesis and the metabolism of lipids in bovine milk is regulated at the miRNA level, primary mammary epithelial cells (pMEC) derived from two Chinese Holstein dairy cows that produced extrem...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17020200
更新日期：2016-02-17 00:00:00
abstract：:Persistent cell shrinkage, called apoptotic volume decrease (AVD), is a pivotal event of apoptosis. Activation of the volume-sensitive outwardly rectifying Cl(-) channel (VSOR) is involved in the AVD induction. On the other hand, activation of the MAP kinase (MAPK) cascade is also known to play a critical role in apop...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13079363
更新日期：2012-01-01 00:00:00
abstract：:Cell-to-cell communication mediates a plethora of cellular decisions and behaviors that are crucial for the correct and robust development of multicellular organisms. Many of these signals are encoded in secreted hormones or growth factors that bind to and activate cell surface receptors, to transmit the cue intracell...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21010188
更新日期：2019-12-26 00:00:00
abstract：:Many studies have provided evidence suggesting that caspases not only contribute to the neurodegeneration associated with Alzheimer's disease (AD) but also play essential roles in promoting the underlying pathology of this disease. Studies regarding the caspase inhibition draw researchers' attention through time due t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20194850
更新日期：2019-09-29 00:00:00
abstract：:Fourier transform infrared spectroscopy (FTIR) can provide crucial information on the molecular structure of organic and inorganic components and has been used extensively for chemical characterization of geological samples in the past few decades. In this paper, recent applications of FTIR in the geological sciences ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161226227
更新日期：2015-12-18 00:00:00
