Abstract:
:Flagellin is amongst the most abundant proteins in flagellated bacterial species and constitutes the major building block of the flagellar filament. The proteins FliW and FliS serve in the post-transcriptional control of flagellin and guide the protein to the flagellar type III secretion system (fT3SS), respectively. Here, we present the high-resolution structure of FliS/flagellin heterodimer and show that FliS and FliW bind to opposing interfaces located at the N- and C-termini of flagellin. The FliS/flagellin/FliW heterotrimer is able to interact with FlhA-C suggesting that FliW and FliS are released during flagellin export. After release, FliW and FliS are recycled to execute a new round of post-transcriptional regulation and targeting. Taken together, our study provides a mechanism explaining how FliW and FliS synchronize the production of flagellin with the capacity of the fT3SS to secrete flagellin.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Altegoer F,Mukherjee S,Steinchen W,Bedrunka P,Linne U,Kearns DB,Bange Gdoi
10.1038/s41598-018-29884-8subject
Has Abstractpub_date
2018-08-01 00:00:00pages
11552issue
1issn
2045-2322pii
10.1038/s41598-018-29884-8journal_volume
8pub_type
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