Abstract:
:The freshwater Testudine species have gained importance in recent years, as most of their population is threatened due to exploitation for delicacy and pet trade. In this regard, Lissemys punctata, a freshwater terrapin, predominantly distributed in Asian countries has gained its significance for the study. A pilot study report on mitochondrial markers (Cyt b and D-loop) conducted on L. punctata species from southern Karnataka, India was presented in this investigation. A complete region spanning 1.14 kb and ∼1 kb was amplified by HotStart PCR and sequenced by Sanger sequencing. The Cyt b sequence revealed 85 substitution sites, no indels and 17 parsimony informative sites, whereas D-loop showed 189 variable sites, 51 parsimony informative sites with 5' functional domains TAS, CSB-F, CSBs (1, 2, 3) preceding tandem repeat at 3' end. Current data highlights the intraspecific variations in these target regions and variations validated using suitable evolutionary models points out that the overall point mutations observed in the region are transitions leading to no structural and functional alterations. The mitochondrial data generated uncover the genetic diversity within species and conservationist can utilize the data to estimate the effective population size or for forensic identification of animal or its seizures during unlawful trade activities.
journal_name
J Adv Resjournal_title
Journal of advanced researchauthors
Lalitha R,Chandavar VRdoi
10.1016/j.jare.2017.10.007subject
Has Abstractpub_date
2017-11-21 00:00:00pages
87-95eissn
2090-1232issn
2090-1224pii
S2090-1232(17)30111-Xjournal_volume
9pub_type
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journal_title:Journal of advanced research
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journal_title:Journal of advanced research
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journal_title:Journal of advanced research
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