Abstract:
:Cryptosporidiosis in an enteric infection caused by Cryptosporidium parasites and is a major cause of acute infant diarrhea in the developing world. A major bottleneck to research progress is the lack of methods to cryopreserve Cryptosporidium oocysts, thus requiring routine propagation in laboratory animals. Here, we report a method to cryopreserve C. parvum oocysts by ultra-fast cooling. Cryopreserved oocysts exhibit high viability and robust in vitro excystation, and are infectious to interferon-γ knockout mice. The course of the infection is comparable to what we observe with unfrozen oocysts. Oocyst viability and infectivity is not visibly changed after several weeks of cryogenic storage. Cryopreservation will facilitate the sharing of oocysts from well-characterized isolates and transgenic strains among different laboratories.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Jaskiewicz JJ,Sandlin RD,Swei AA,Widmer G,Toner M,Tzipori Sdoi
10.1038/s41467-018-05240-2subject
Has Abstractpub_date
2018-07-23 00:00:00pages
2883issue
1issn
2041-1723pii
10.1038/s41467-018-05240-2journal_volume
9pub_type
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