Cryopreservation of infectious Cryptosporidium parvum oocysts.

Abstract:

:Cryptosporidiosis in an enteric infection caused by Cryptosporidium parasites and is a major cause of acute infant diarrhea in the developing world. A major bottleneck to research progress is the lack of methods to cryopreserve Cryptosporidium oocysts, thus requiring routine propagation in laboratory animals. Here, we report a method to cryopreserve C. parvum oocysts by ultra-fast cooling. Cryopreserved oocysts exhibit high viability and robust in vitro excystation, and are infectious to interferon-γ knockout mice. The course of the infection is comparable to what we observe with unfrozen oocysts. Oocyst viability and infectivity is not visibly changed after several weeks of cryogenic storage. Cryopreservation will facilitate the sharing of oocysts from well-characterized isolates and transgenic strains among different laboratories.

journal_name

Nat Commun

journal_title

Nature communications

authors

Jaskiewicz JJ,Sandlin RD,Swei AA,Widmer G,Toner M,Tzipori S

doi

10.1038/s41467-018-05240-2

subject

Has Abstract

pub_date

2018-07-23 00:00:00

pages

2883

issue

1

issn

2041-1723

pii

10.1038/s41467-018-05240-2

journal_volume

9

pub_type

杂志文章
  • Active suppression of intestinal CD4(+)TCRαβ(+) T-lymphocyte maturation during the postnatal period.

    abstract::Priming of the mucosal immune system during the postnatal period substantially influences host-microbial interaction and susceptibility to immune-mediated diseases in adult life. The underlying mechanisms are ill defined. Here we show that shortly after birth, CD4 T cells populate preformed lymphoid structures in the ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms8725

    authors: Torow N,Yu K,Hassani K,Freitag J,Schulz O,Basic M,Brennecke A,Sparwasser T,Wagner N,Bleich A,Lochner M,Weiss S,Förster R,Pabst O,Hornef MW

    更新日期:2015-07-21 00:00:00

  • Coral-associated bacteria demonstrate phylosymbiosis and cophylogeny.

    abstract::Scleractinian corals' microbial symbionts influence host health, yet how coral microbiomes assembled over evolution is not well understood. We survey bacterial and archaeal communities in phylogenetically diverse Australian corals representing more than 425 million years of diversification. We show that coral microbio...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-07275-x

    authors: Pollock FJ,McMinds R,Smith S,Bourne DG,Willis BL,Medina M,Thurber RV,Zaneveld JR

    更新日期:2018-11-22 00:00:00

  • Vapour-phase-transport rearrangement technique for the synthesis of new zeolites.

    abstract::Owing to the significant difference in the numbers of simulated and experimentally feasible zeolite structures, several alternative strategies have been developed for zeolite synthesis. Despite their rationality and originality, most of these techniques are based on trial-and-error, which makes it difficult to predict...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-12882-3

    authors: Kasneryk V,Shamzhy M,Zhou J,Yue Q,Mazur M,Mayoral A,Luo Z,Morris RE,Čejka J,Opanasenko M

    更新日期:2019-11-12 00:00:00

  • Terahertz-driven linear electron acceleration.

    abstract::The cost, size and availability of electron accelerators are dominated by the achievable accelerating gradient. Conventional high-brightness radio-frequency accelerating structures operate with 30-50 MeV m(-1) gradients. Electron accelerators driven with optical or infrared sources have demonstrated accelerating gradi...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms9486

    authors: Nanni EA,Huang WR,Hong KH,Ravi K,Fallahi A,Moriena G,Miller RJ,Kärtner FX

    更新日期:2015-10-06 00:00:00

  • Ribosomal DNA copy number is coupled with gene expression variation and mitochondrial abundance in humans.

    abstract::Ribosomes are essential intracellular machines composed of proteins and RNA molecules. The DNA sequences (rDNA) encoding ribosomal RNAs (rRNAs) are tandemly repeated and give origin to the nucleolus. Here we develop a computational method for estimating rDNA dosage (copy number) and mitochondrial DNA abundance using w...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms5850

    authors: Gibbons JG,Branco AT,Yu S,Lemos B

    更新日期:2014-09-11 00:00:00

  • ATF3 acts as a rheostat to control JNK signalling during intestinal regeneration.

    abstract::Epithelial barrier function is maintained by coordination of cell proliferation and cell loss, whereas barrier dysfunction can lead to disease and organismal death. JNK signalling is a conserved stress signalling pathway activated by bacterial infection and tissue damage, often leading to apoptotic cell death and comp...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms14289

    authors: Zhou J,Edgar BA,Boutros M

    更新日期:2017-03-08 00:00:00

  • Direct observation of coordinated DNA movements on the nucleosome during chromatin remodelling.

    abstract::ATP-dependent chromatin remodelling enzymes (remodellers) regulate DNA accessibility in eukaryotic genomes. Many remodellers reposition (slide) nucleosomes, however, how DNA is propagated around the histone octamer during this process is unclear. Here we examine the real-time coordination of remodeller-induced DNA mov...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-09657-1

    authors: Sabantsev A,Levendosky RF,Zhuang X,Bowman GD,Deindl S

    更新日期:2019-04-12 00:00:00

  • Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA.

    abstract::Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template muc...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-14200-3

    authors: Kang W,Ha KS,Uhm H,Park K,Lee JY,Hohng S,Kang C

    更新日期:2020-01-23 00:00:00

  • Caveolin-1-mediated sphingolipid oncometabolism underlies a metabolic vulnerability of prostate cancer.

    abstract::Plasma and tumor caveolin-1 (Cav-1) are linked with disease progression in prostate cancer. Here we report that metabolomic profiling of longitudinal plasmas from a prospective cohort of 491 active surveillance (AS) participants indicates prominent elevations in plasma sphingolipids in AS progressors that, together wi...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-17645-z

    authors: Vykoukal J,Fahrmann JF,Gregg JR,Tang Z,Basourakos S,Irajizad E,Park S,Yang G,Creighton CJ,Fleury A,Mayo J,Paulucci-Holthauzen A,Dennison JB,Murage E,Peterson CB,Davis JW,Kim J,Hanash S,Thompson TC

    更新日期:2020-08-27 00:00:00

  • Reconstitution of the complete pathway of ITS2 processing at the pre-ribosome.

    abstract::Removal of internal transcribed spacer 2 (ITS2) from pre-ribosomal RNA is essential to make functional ribosomes. This complicated processing reaction begins with a single endonucleolytic cleavage followed by exonucleolytic trimming at both new cleavage sites to generate mature 5.8S and 25S rRNA. We reconstituted the ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-01786-9

    authors: Fromm L,Falk S,Flemming D,Schuller JM,Thoms M,Conti E,Hurt E

    更新日期:2017-11-27 00:00:00

  • Reconstructing neuronal circuitry from parallel spike trains.

    abstract::State-of-the-art techniques allow researchers to record large numbers of spike trains in parallel for many hours. With enough such data, we should be able to infer the connectivity among neurons. Here we develop a method for reconstructing neuronal circuitry by applying a generalized linear model (GLM) to spike cross-...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-12225-2

    authors: Kobayashi R,Kurita S,Kurth A,Kitano K,Mizuseki K,Diesmann M,Richmond BJ,Shinomoto S

    更新日期:2019-10-02 00:00:00

  • Tau association with synaptic vesicles causes presynaptic dysfunction.

    abstract::Tau is implicated in more than 20 neurodegenerative diseases, including Alzheimer's disease. Under pathological conditions, Tau dissociates from axonal microtubules and missorts to pre- and postsynaptic terminals. Patients suffer from early synaptic dysfunction prior to Tau aggregate formation, but the underlying mech...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms15295

    authors: Zhou L,McInnes J,Wierda K,Holt M,Herrmann AG,Jackson RJ,Wang YC,Swerts J,Beyens J,Miskiewicz K,Vilain S,Dewachter I,Moechars D,De Strooper B,Spires-Jones TL,De Wit J,Verstreken P

    更新日期:2017-05-11 00:00:00

  • Modified relaxation dynamics and coherent energy exchange in coupled vibration-cavity polaritons.

    abstract::Coupling vibrational transitions to resonant optical modes creates vibrational polaritons shifted from the uncoupled molecular resonances and provides a convenient way to modify the energetics of molecular vibrations. This approach is a viable method to explore controlling chemical reactivity. In this work, we report ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms13504

    authors: Dunkelberger AD,Spann BT,Fears KP,Simpkins BS,Owrutsky JC

    更新日期:2016-11-22 00:00:00

  • Antidromic-rectifying gap junctions amplify chemical transmission at functionally mixed electrical-chemical synapses.

    abstract::Neurons communicate through chemical synapses and electrical synapses (gap junctions). Although these two types of synapses often coexist between neurons, little is known about whether they interact, and whether any interactions between them are important to controlling synaptic strength and circuit functions. By stud...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms14818

    authors: Liu P,Chen B,Mailler R,Wang ZW

    更新日期:2017-03-20 00:00:00

  • Controllable conversion of quasi-freestanding polymer chains to graphene nanoribbons.

    abstract::In the bottom-up synthesis of graphene nanoribbons (GNRs) from self-assembled linear polymer intermediates, surface-assisted cyclodehydrogenations usually take place on catalytic metal surfaces. Here we demonstrate the formation of GNRs from quasi-freestanding polymers assisted by hole injections from a scanning tunne...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms14815

    authors: Ma C,Xiao Z,Zhang H,Liang L,Huang J,Lu W,Sumpter BG,Hong K,Bernholc J,Li AP

    更新日期:2017-03-13 00:00:00

  • Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes in yeast.

    abstract::Transcription initiation involves the coordinated activities of large multimeric complexes, but little is known about their biogenesis. Here we report several principles underlying the assembly and topological organization of the highly conserved SAGA and NuA4 co-activator complexes, which share the Tra1 subunit. We s...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-13243-w

    authors: Elías-Villalobos A,Toullec D,Faux C,Séveno M,Helmlinger D

    更新日期:2019-11-20 00:00:00

  • High conductance values in π-folded molecular junctions.

    abstract::Folding processes play a crucial role in the development of function in biomacromolecules. Recreating this feature on synthetic systems would not only allow understanding and reproducing biological functions but also developing new functions. This has inspired the development of conformationally ordered synthetic olig...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms15195

    authors: Carini M,Ruiz MP,Usabiaga I,Fernández JA,Cocinero EJ,Melle-Franco M,Diez-Perez I,Mateo-Alonso A

    更新日期:2017-05-18 00:00:00

  • Ice rule fragility via topological charge transfer in artificial colloidal ice.

    abstract::Artificial particle ices are model systems of constrained, interacting particles. They have been introduced theoretically to study ice-manifolds emergent from frustration, along with domain wall and grain boundary dynamics, doping, pinning-depinning, controlled transport of topological defects, avalanches, and memory ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-06631-1

    authors: Libál A,Lee DY,Ortiz-Ambriz A,Reichhardt C,Reichhardt CJO,Tierno P,Nisoli C

    更新日期:2018-10-08 00:00:00

  • Tyrosine-mediated two-dimensional peptide assembly and its role as a bio-inspired catalytic scaffold.

    abstract::In two-dimensional interfacial assemblies, there is an interplay between molecular ordering and interface geometry, which determines the final morphology and order of entire systems. Here we present the interfacial phenomenon of spontaneous facet formation in a water droplet driven by designed peptide assembly. The id...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms4665

    authors: Jang HS,Lee JH,Park YS,Kim YO,Park J,Yang TY,Jin K,Lee J,Park S,You JM,Jeong KW,Shin A,Oh IS,Kwon MK,Kim YI,Cho HH,Han HN,Kim Y,Chang YH,Paik SR,Nam KT,Lee YS

    更新日期:2014-04-11 00:00:00

  • Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.

    abstract::Breast cancer metastasis involves lymphatic dissemination in addition to hematogenous spreading. Although stromal lymphatic vessels (LVs) serve as initial metastatic routes, roles of organ-residing LVs are underinvestigated. Here we show that lymphatic endothelial cells (LECs), a component of LVs within pre-metastatic...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms5715

    authors: Lee E,Fertig EJ,Jin K,Sukumar S,Pandey NB,Popel AS

    更新日期:2014-09-02 00:00:00

  • TAF1 plays a critical role in AML1-ETO driven leukemogenesis.

    abstract::AML1-ETO (AE) is a fusion transcription factor, generated by the t(8;21) translocation, that functions as a leukemia promoting oncogene. Here, we demonstrate that TATA-Box Binding Protein Associated Factor 1 (TAF1) associates with K43 acetylated AE and this association plays a pivotal role in the proliferation of AE-e...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-12735-z

    authors: Xu Y,Man N,Karl D,Martinez C,Liu F,Sun J,Martinez CJ,Martin GM,Beckedorff F,Lai F,Yue J,Roisman A,Greenblatt S,Duffort S,Wang L,Sun X,Figueroa M,Shiekhattar R,Nimer S

    更新日期:2019-10-29 00:00:00

  • The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma.

    abstract::FAM46C is one of the most frequently mutated genes in multiple myeloma. Here, using a combination of in vitro and in vivo approaches, we demonstrate that FAM46C encodes an active non-canonical poly(A) polymerase which enhances mRNA stability and gene expression. Reintroduction of active FAM46C into multiple myeloma ce...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-00578-5

    authors: Mroczek S,Chlebowska J,Kuliński TM,Gewartowska O,Gruchota J,Cysewski D,Liudkovska V,Borsuk E,Nowis D,Dziembowski A

    更新日期:2017-09-20 00:00:00

  • Broadband multi-magnon relaxometry using a quantum spin sensor for high frequency ferromagnetic dynamics sensing.

    abstract::Development of sensitive local probes of magnon dynamics is essential to further understand the physical processes that govern magnon generation, propagation, scattering, and relaxation. Quantum spin sensors like the NV center in diamond have long spin lifetimes and their relaxation can be used to sense magnetic field...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-19121-0

    authors: McCullian BA,Thabt AM,Gray BA,Melendez AL,Wolf MS,Safonov VL,Pelekhov DV,Bhallamudi VP,Page MR,Hammel PC

    更新日期:2020-10-16 00:00:00

  • Synthetic TRuC receptors engaging the complete T cell receptor for potent anti-tumor response.

    abstract::T cells expressing CD19-targeting chimeric antigen receptors (CARs) reveal high efficacy in the treatment of B cell malignancies. Here, we report that T cell receptor fusion constructs (TRuCs) comprising an antibody-based binding domain fused to T cell receptor (TCR) subunits can effectively reprogram an intact TCR co...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-10097-0

    authors: Baeuerle PA,Ding J,Patel E,Thorausch N,Horton H,Gierut J,Scarfo I,Choudhary R,Kiner O,Krishnamurthy J,Le B,Morath A,Baldeviano GC,Quinn J,Tavares P,Wei Q,Weiler S,Maus MV,Getts D,Schamel WW,Hofmeister R

    更新日期:2019-05-07 00:00:00

  • Ultraflexible organic amplifier with biocompatible gel electrodes.

    abstract::In vivo electronic monitoring systems are promising technology to obtain biosignals with high spatiotemporal resolution and sensitivity. Here we demonstrate the fabrication of a biocompatible highly conductive gel composite comprising multi-walled carbon nanotube-dispersed sheet with an aqueous hydrogel. This gel comp...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms11425

    authors: Sekitani T,Yokota T,Kuribara K,Kaltenbrunner M,Fukushima T,Inoue Y,Sekino M,Isoyama T,Abe Y,Onodera H,Someya T

    更新日期:2016-04-29 00:00:00

  • Dual nature of magnetic dopants and competing trends in topological insulators.

    abstract::Topological insulators interacting with magnetic impurities have been reported to host several unconventional effects. These phenomena are described within the framework of gapping Dirac quasiparticles due to broken time-reversal symmetry. However, the overwhelming majority of studies demonstrate the presence of a fin...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms12027

    authors: Sessi P,Biswas RR,Bathon T,Storz O,Wilfert S,Barla A,Kokh KA,Tereshchenko OE,Fauth K,Bode M,Balatsky AV

    更新日期:2016-06-27 00:00:00

  • Persistent drying in the tropics linked to natural forcing.

    abstract::Approximately half of the world's population lives in the tropics, and future changes in the hydrological cycle will impact not just the freshwater supplies but also energy production in areas dependent upon hydroelectric power. It is vital that we understand the mechanisms/processes that affect tropical precipitation...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms8627

    authors: Winter A,Zanchettin D,Miller T,Kushnir Y,Black D,Lohmann G,Burnett A,Haug GH,Estrella-Martínez J,Breitenbach SF,Beaufort L,Rubino A,Cheng H

    更新日期:2015-07-14 00:00:00

  • Spatially selective manipulation of cells with single-beam acoustical tweezers.

    abstract::Acoustical tweezers open major prospects in microbiology for cells and microorganisms contactless manipulation, organization and mechanical properties testing since they are biocompatible, label-free and have the potential to exert forces several orders of magnitude larger than their optical counterpart at equivalent ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-18000-y

    authors: Baudoin M,Thomas JL,Sahely RA,Gerbedoen JC,Gong Z,Sivery A,Matar OB,Smagin N,Favreau P,Vlandas A

    更新日期:2020-08-25 00:00:00

  • Dendritic core-shell nickel-iron-copper metal/metal oxide electrode for efficient electrocatalytic water oxidation.

    abstract::Electrochemical water splitting requires efficient water oxidation catalysts to accelerate the sluggish kinetics of water oxidation reaction. Here, we report a promisingly dendritic core-shell nickel-iron-copper metal/metal oxide electrode, prepared via dealloying with an electrodeposited nickel-iron-copper alloy as a...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-017-02429-9

    authors: Zhang P,Li L,Nordlund D,Chen H,Fan L,Zhang B,Sheng X,Daniel Q,Sun L

    更新日期:2018-01-26 00:00:00

  • Dynamic nuclear magnetic resonance field sensing with part-per-trillion resolution.

    abstract::High-field magnets of up to tens of teslas in strength advance applications in physics, chemistry and the life sciences. However, progress in generating such high fields has not been matched by corresponding advances in magnetic field measurement. Based mostly on nuclear magnetic resonance, dynamic high-field magnetom...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms13702

    authors: Gross S,Barmet C,Dietrich BE,Brunner DO,Schmid T,Pruessmann KP

    更新日期:2016-12-02 00:00:00