Abstract:
:The centromere binding factor 3 (CBF3) complex binds the third centromere DNA element in organisms with point centromeres, such as S. cerevisiae. It is an essential complex for assembly of the kinetochore in these organisms, as it facilitates genetic centromere specification and allows association of all other kinetochore components. We determined high-resolution structures of the core complex of CBF3 alone and in association with a monomeric construct of Ndc10, using cryoelectron microscopy (cryo-EM). We identify the DNA-binding site of the complex and present a model in which CBF3 induces a tight bend in centromeric DNA, thus facilitating assembly of the centromeric nucleosome.
journal_name
Cell Repjournal_title
Cell reportsauthors
Zhang W,Lukoyanova N,Miah S,Lucas J,Vaughan CKdoi
10.1016/j.celrep.2018.06.068subject
Has Abstractpub_date
2018-07-17 00:00:00pages
744-754issue
3issn
2211-1247pii
S2211-1247(18)30995-1journal_volume
24pub_type
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