Abstract：
BACKGROUND:Epstein-Barr Virus (EBV) positive and microsatellite unstable (MSI-high) gastric cancer (GC) are molecular subgroups with distinctive molecular profiles. We explored the transcriptomic differences between EBV+ and MSI-high GCs, and the expression of current GC immunotherapy targets such as PD-1, PD-L1, CTLA4 and Dies1/VISTA. METHODS:Using Nanostring Technology and comparative bioinformatics, we analyzed the expression of 499 genes in 46 GCs, classified either as EBV positive (EBER in situ hybridization) or MSI-high (PCR/fragment analysis). PD-L1 protein expression was assessed by immunohistochemistry. RESULTS:From the 46 GCs, 27 tested MSI-high/EBV-, 15 tested MSS/EBV+ and four tested MSS/EBV-. The Nanostring CodeSet could segregate GCs according to MSI and, to a lesser extent, EBV status. Functional annotation of differentially expressed genes associated MSI-high/EBV- GCs with mitotic activity and MSS/EBV+ GCs with immune response. PD-L1 protein expression, evaluated in stromal immune cells, was lower in MSI-high/EBV- GCs. High mRNA expression of PD-1, CTLA4 and Dies1/VISTA and distinctive PD-1/PD-L1 co-expression patterns (PD-1high/PD-L1low, PD-1high/PDL1high) were associated with MSS/EBV+ molecular subtype and gastric cancer with lymphoid stroma (GCLS) morphological features. CONCLUSIONS:EBV+ and MSI-high GCs present distinct transcriptomic profiles. GCLS/EBV+ cases frequently present co-expression of multiple immunotherapy targets, a finding with putative therapeutic implications.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Gullo I,Carvalho J,Martins D,Lemos D,Monteiro AR,Ferreira M,Das K,Tan P,Oliveira C,Carneiro F,Oliveira Pdoi
10.3390/ijms19072079subject
Has Abstractpub_date
2018-07-17 00:00:00issue
7issn
1422-0067pii
ijms19072079journal_volume
19pub_type
杂志文章abstract：:Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these proteome changes relate to the loss of sperm function during cryopreservation remains unsolved. The present study aimed to clarify this issue evaluating differential changes in the proteome of fresh and frozen-thawed p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20071791
更新日期：2019-04-11 00:00:00
abstract：:Neurodegenerative proteinopathies are complex diseases that share some pathogenetic processes. One of these is the failure of the proteostasis network (PN), which includes all components involved in the synthesis, folding, and degradation of proteins, thus leading to the aberrant accumulation of toxic protein aggregat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21176405
更新日期：2020-09-03 00:00:00
abstract：:Autophagy is a general protective mechanism for maintaining homeostasis in eukaryotic cells, regulating cellular metabolism, and promoting cell survival by degrading and recycling cellular components under stress conditions. The degradation pathway that is mediated by autophagy receptors is called selective autophagy,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21062008
更新日期：2020-03-16 00:00:00
abstract：:Ubiquitination is a reversible post-translational modification involved in a plethora of different physiological functions. Among the substrates that are ubiquitinated, neurotrophin receptors (TrkA, TrkB, TrkC, and p75NTR) have been studied recently. TrkA is the most studied receptor in terms of its ubiquitination, an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18030630
更新日期：2017-03-14 00:00:00
abstract：:Increasing evidence suggests that the gut microbiota plays vital roles in metabolic diseases. Polygonatum odoratum extract alleviates hyperglycemia and hyperlipidemia, but the underlying mechanism remains unclear. This study investigated the effects of P. odoratum polysaccharides (POPs) on high-fat diet (HFD)-induced ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113587
更新日期：2018-11-13 00:00:00
abstract：:Salidroside (Sal) is an active ingredient that is isolated from Rhodiola rosea, which has been reported to have anti-inflammatory activities and a renal protective effect. However, the role of Sal on renal fibrosis has not yet been elucidated. Here, the purpose of the current study is to test the protective effects of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051103
更新日期：2019-03-04 00:00:00
abstract：:Glycation is a non-enzymatic post-translational modification of proteins, formed by the reaction of reducing sugars and α-dicarbonyl products of their degradation with amino and guanidino groups of proteins. Resulted early glycation products are readily involved in further transformation, yielding a heterogeneous grou...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18122557
更新日期：2017-11-28 00:00:00
abstract：:Background: Chronic skin wounds are a growing financial burden for healthcare providers, causing discomfort/immobility to patients. Whilst animal chronic wound models have been developed to allow for mechanistic studies and to develop/test potential therapies, such systems are not good representations of the human chr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041001
更新日期：2018-03-27 00:00:00
abstract：:In this study, the potential bioactivities of Portuguese oyster (Crassostrea angulata) proteins were predicted through in silico analyses and confirmed by in vitro tests. C. angulata proteins were characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and identified by proteomics techni...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205191
更新日期：2019-10-20 00:00:00
abstract：:Bipolar disorder (BPD) and schizophrenia (SCH) show similar neuropsychiatric behavioral disturbances, including impaired social interaction and communication, seen in autism spectrum disorder (ASD) with multiple overlapping genetic and environmental influences implicated in risk and course of illness. GeneAnalytics so...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18030527
更新日期：2017-02-28 00:00:00
abstract：:Growth hormone (GH), mostly through its peripheral mediator, the insulin-like growth factor 1(IGF1), in addition to carrying out its fundamental action to promote linear bone growth, plays an important role throughout life in the regulation of intermediate metabolism, trophism and function of various organs, especiall...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21041392
更新日期：2020-02-19 00:00:00
abstract：:Catalytic decomposition of sucrose by acid invertases (AINVs) under acidic conditions plays an important role in the development of sink organs in plants. To reveal the function of AINVs in the development of pepper fruits, nine AINV genes of pepper were identified. Protein sequencing and phylogenetic analysis reveale...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20010015
更新日期：2018-12-20 00:00:00
abstract：:Stem cells, a special subset of cells derived from embryo or adult tissues, are known to present the characteristics of self-renewal, multiple lineages of differentiation, high plastic capability, and long-term maintenance. Recent reports have further suggested that neural stem cells (NSCs) derived from the adult hipp...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms11052039
更新日期：2010-05-05 00:00:00
abstract：:Protein-protein interactions are important for the molecular understanding of the biological processes of proteins. The dimerization of bZIPs (basic leucine zipper proteins) is involved in modifying binding site specificities, altering dimer stability, and permitting a new set of specific protein-to-protein interactio...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150610005
更新日期：2014-06-04 00:00:00
abstract：:Milk fat is a key factor affecting milk quality and is also a major trait targeted in dairy cow breeding. To determine how the synthesis and the metabolism of lipids in bovine milk is regulated at the miRNA level, primary mammary epithelial cells (pMEC) derived from two Chinese Holstein dairy cows that produced extrem...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17020200
更新日期：2016-02-17 00:00:00
abstract：:Autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and psoriatic arthritis (PsA) are chronic inflammatory disorders of unknown etiology characterized by a wide range of abnormalities of the immune system that may compromise the function of several organs, such as kidney, heart, j...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms15034965
更新日期：2014-03-20 00:00:00
abstract：:Obesity is a major risk for diabetes. Brown adipose tissue (BAT) mediates production of heat while white adipose tissue (WAT) function in the storage of fat. Roles of BAT in the treatment of obesity and related disorders warrants more investigation. Peroxisome proliferator activator receptor gamma (PPAR-γ) is the mast...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20071618
更新日期：2019-04-01 00:00:00
abstract：:The iliotibial band (ITB) is a suitable scaffold for anterior cruciate ligament (ACL) reconstruction, providing a sufficient mechanical resistance to loading. Hence, ITB-derived fibroblasts attract interest for ligament tissue engineering but have so far not been characterized. This present study aimed at characterizi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081972
更新日期：2019-04-22 00:00:00
abstract：:Metacaspases, a class of cysteine-dependent proteases like caspases in animals, are important regulators of programmed cell death (PCD) during development and stress responses in plants. The present study was focused on comprehensive analyses of expression patterns of the rice metacaspase (OsMC) genes in response to a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160716216
更新日期：2015-07-17 00:00:00
abstract：:Skeletal muscles respond to environmental and physiological changes by varying their size, fiber type, and metabolic properties. P38 mitogen-activated protein kinase (MAPK) is one of several signaling pathways that drive the metabolic adaptation of skeletal muscle to exercise. p38 MAPK also participates in the develop...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21186480
更新日期：2020-09-04 00:00:00
abstract：:Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21155269
更新日期：2020-07-24 00:00:00
abstract：:Epidemiological results revealed that there is an inverse correlation between high-density lipoprotein (HDL) cholesterol levels and risks of atherosclerotic cardiovascular disease (ASCVD). Mounting evidence supports that HDLs are atheroprotective, therefore, many therapeutic approaches have been developed to increase ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21030739
更新日期：2020-01-23 00:00:00
abstract：:A field experiment was conducted using a 3 × 3 orthogonal regression design to explore the growth promotion of one-year-old Yunnan pine seedlings (Pinus yunnanensis Franch.) in response to foliar application of IAA (indole-3-acetic acid) at rates of 0, 200 and 400 mg·L(-1) and IBA (indole-3-butyric acid) at rates of 0...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13056507
更新日期：2012-01-01 00:00:00
abstract：:Senescence is the final stage of leaf development which is accompanied by highly coordinated and complicated reprogramming of gene expression. Genetic manipulation of leaf senescence in major crops including wheat has been shown to be able to increase stress tolerance and grain yield. NAC(No apical meristem (NAM), ATA...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21207672
更新日期：2020-10-16 00:00:00
abstract：:Benzo[a]pyrene (BaP) is a well-known carcinogen formed during the cooking process. Although BaP exposure has been implicated as one of the risk factors for lung cancer in animals and humans, there are only limited data on BaP-induced gastrointestinal cancer. Therefore, this study investigated the protective effects of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20225533
更新日期：2019-11-06 00:00:00
abstract：:Methyl jasmonate (MeJA), synthesized in the jasmonic acid (JA) pathway, has been found to upregulate glucosinolate (GS) biosynthesis in plant species of the Brassicaceae family. Exogenous application of MeJA has shown to increase tissue GS concentrations and the formation of myrosinase-mediated GS hydrolysis products ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041058
更新日期：2018-04-02 00:00:00
abstract：:Scaffolds made of biodegradable biomaterials are widely used to guide bone regeneration. Commonly, in vitro assessment of scaffolds' osteogenesis potential has been performed predominantly in monoculture settings. Hence, this study evaluated the potential of an unstimulated, growth factor-free co-culture system compri...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051068
更新日期：2019-03-01 00:00:00
abstract：:Nosocomial (hospital-acquired) infections remain a serious challenge for health systems. The reason for this lies not only in the local imperfection of medical practices and protocols. The frequency of infection with antibiotic-resistant strains of bacteria is growing every year, both in developed and developing count...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21217839
更新日期：2020-10-22 00:00:00
abstract：:Among popular crops, plum (Prunus domestica L.) has received special attention due to its health-promoting properties. The seasonality of this fruit makes it impossible to consume it throughout the year, so new products in a powder form may offer an alternative to fresh consumption and may be used as high-quality natu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18010176
更新日期：2017-01-17 00:00:00
abstract：:S-equol is a major bacterial metabolite of the soy isoflavone daidzein. It is known to be a phytoestrogen that acts by binding to the nuclear estrogen receptors (ERs) that are expressed in various brain regions, including the cerebellum. However, the effects of S-equol on cerebellar development and function have not y...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205178
更新日期：2019-10-18 00:00:00
