Use of GH4C1 cell variants to demonstrate a non-spare receptor model for thyrotropin-releasing hormone action.

abstract：:As part of an ongoing study of the cell-specific expression of glandular kallikrein genes in mice, we have investigated cellular sites of expression of the renal/pancreatic kallikrein gene, mGK-6, during fetal life. Expression of alpha I and beta-subunit genes of Na+K+ATPase and bradykinin binding were used as an indi...

journal_title：Molecular and cellular endocrinology

authors： Penschow JD,Coghlan JP

更新日期：1992-10-01 00:00:00

abstract：:This report represents the joint efforts of three laboratories, one with a primary interest in understanding regulatory processes in the epididymal epithelium (TTT) and two with a primary interest in identifying and characterizing new contraceptive targets (DSJ and SAJ). We have developed a highly refined mouse epidid...

journal_title：Molecular and cellular endocrinology

authors： Turner TT,Johnston DS,Jelinsky SA

更新日期：2006-05-16 00:00:00

abstract：:Interleukin-1 has been shown to contribute to infection-induced inflammatory processes during pregnancy. Prior work from this laboratory has demonstrated that serotonin-induced IL-1alpha also is required for the in-vitro production of collagenase in uterine smooth muscle cells, a normal, non-inflammatory process that ...

journal_title：Molecular and cellular endocrinology

authors： Huang TT,Vinci JM,Lan L,Jeffrey JJ,Wilcox BD

更新日期：1999-06-25 00:00:00

abstract：:Over the last 20 years, aromatase inhibitors have been developed to become a highly effective treatment strategy for treatment of hormone receptor positive breast cancer. Despite their success, poor response and resistance limit the effectiveness of these agents in up to 50% of patients. In recent years, studies using...

journal_title：Molecular and cellular endocrinology

authors： Dunbier AK,Martin LA,Dowsett M

更新日期：2011-07-04 00:00:00

abstract：:Obesity patients are more susceptible to develop COVID-19 severe outcome due to the role of angiotensin-converting enzyme 2 (ACE2) in the viral infection. ACE2 is regulated in the human cells by different genes associated with increased (TLR3, HAT1, HDAC2, KDM5B, SIRT1, RAB1A, FURIN and ADAM10) or decreased (TRIB3) vi...

journal_title：Molecular and cellular endocrinology

authors： de Oliveira M,De Sibio MT,Mathias LS,Rodrigues BM,Sakalem ME,Nogueira CR

更新日期：2020-09-15 00:00:00

abstract：:1,25-Dihydroxyvitamin D(3) (1,25D(3)), the active metabolite of vitamin D(3), inhibits breast cancer cell growth in vivo and in vitro. To examine mechanisms of 1,25D(3) induced growth arrest and apoptosis, cell lines were established from DMBA induced mammary tumors derived from vitamin D(3) receptor knockout (VDRKO) ...

journal_title：Molecular and cellular endocrinology

authors： Zinser GM,McEleney K,Welsh J

更新日期：2003-02-28 00:00:00

abstract：:Glucose-dependent insulinotropic polypeptide (GIP) plays an important role in stimulating insulin release in the pancreas as well as inhibiting gastric acid secretion in the stomach. GIP has been found in specific endocrine cells located in the mucosal layer of the small intestine and in the submandibular salivary gla...

journal_title：Molecular and cellular endocrinology

authors： Yeung CM,Wong CK,Chung SK,Chung SS,Chow BK

更新日期：1999-08-20 00:00:00

abstract：:Using as a model system, primary cultures of porcine Leydig cells, we have shown that transforming growth factor-beta 1 (TGF-beta 1) (2 ng/ml, 72 h) antagonizes the stimulatory action of insulin-like growth factor-I (IGF-I) on luteinizing hormone (LH/hCG) receptors. We therefore investigated the action of TGF-beta 1 o...

journal_title：Molecular and cellular endocrinology

authors： Besset V,Collette J,Chauvin MA,Franchimont P,Benahmed M

更新日期：1994-03-01 00:00:00

abstract：:A kallikrein-related protease was purified from rat ventral prostate cytosol by means of DEAE-Sepharose chromatography, followed by gel filtration on Sephadex G-100 and CM-cellulose chromatography. Antibodies raised in rabbits against the purified protease recognize two bands on immunoblots of prostatic cytosol: a 31,...

journal_title：Molecular and cellular endocrinology

authors： Winderickx J,Swinnen K,Van Dijck P,Verhoeven G,Heyns W

更新日期：1989-04-01 00:00:00

abstract：:Sox genes participate in several developmental processes, including sex determination and differentiation. In this study, the genomic structure of sox17 was characterized in the sea bass (sb). Two transcripts, one producing a normal protein (sb Sox17) and another producing a truncated protein (sb t-Sox17) were detecte...

journal_title：Molecular and cellular endocrinology

authors： Navarro-Martín L,Galay-Burgos M,Sweeney G,Piferrer F

更新日期：2009-02-27 00:00:00

abstract：:Placental leptin secretion has important implications for maternal adaptation to pregnancy, fetal growth and development, and local autocrine/paracrine actions within trophoblast. In this study we used a cell culture insert model to examine directional secretion of leptin from the basal and apical surfaces of human ch...

journal_title：Molecular and cellular endocrinology

authors： Wyrwoll CS,Mark PJ,Waddell BJ

更新日期：2005-09-28 00:00:00

abstract：:The CYP11A1 gene encodes cytochrome P450scc, the enzyme catalyzing the first step of steroid biosynthesis in the adrenal and gonad. We generated transgenic mice containing 2.3 kb of the 5'-flanking region of CYP11A1 driving LacZ reporter gene expression, in order to study hormonal control of CYP11A1 gene expression in...

journal_title：Molecular and cellular endocrinology

authors： Huang Y,Hu M,Hsu N,Wang CL,Chung B

更新日期：2001-04-25 00:00:00

abstract：:A thyroid-specific enhancer element located upstream from the bovine thyroglobulin gene had been shown to contain three contiguous regions that are protected by thyroid transcription factor 1 (TTF-1) in footprinting experiments in vitro. The functional relevance of the individual TTF-1 binding sites was investigated i...

journal_title：Molecular and cellular endocrinology

authors： Christophe-Hobertus C,Christophe D

更新日期：1999-03-25 00:00:00

abstract：:Testes from rats of different maturational ages were explored for presence of paracrine sertoli cell growth factors. Pubertal and adult testes contained a 17 kDa protein, with potent stimulatory effect on immature Sertoli cell multiplication in vitro. The bioactivity of this protein was mimicked by rat interleukin-1 (...

journal_title：Molecular and cellular endocrinology

authors： Petersen C,Boitani C,Fröysa B,Söder O

更新日期：2002-01-15 00:00:00

abstract：:Receptor-mediated Ca2+ entry was investigated in fura-2-loaded thyroid FRTL-5 cells. Activation of protein kinase C (PKC) by phorbol myristate acetate (PMA) attenuated the ATP-induced increase in intracellular free Ca2+ ([Ca2+]i). In PKC down-regulated cells, the ATP-induced increase in [Ca2+]i was increased compared ...

journal_title：Molecular and cellular endocrinology

authors： Törnquist K

更新日期：1993-05-01 00:00:00

abstract：:Androgen receptor mRNA was translated in vitro, and androgen- and antiandrogen-bound receptor complexes were studied using limited proteolytic digestion by trypsin. Partial proteolysis of androgen-bound receptor protein resulted in a 29-kDa proteolysis-resisting fragment, whereas antiandrogen binding stabilised a 35-k...

journal_title：Molecular and cellular endocrinology

authors： Kuil CW,Mulder E

更新日期：1994-06-01 00:00:00

abstract：:The purpose of this paper is to review potential novel functional pathways by which estradiol and estrogenic compounds elicit biological responses in mammals. We will limit our approach to those novel functions suggested by phenotypes associated with estrogen receptor-alpha (ER alpha) gene mutations and polymorphisms....

journal_title：Molecular and cellular endocrinology

authors： Taylor JA,Lewis KJ,Lubahn DB

更新日期：1998-10-25 00:00:00

abstract：:This study demonstrates that a single subcutaneous injection of gonadotrophin-releasing hormone (GnRH) (60 ng) to GnRH-deficient (hpg) male mice causes a doubling of pituitary GnRH receptors (GnRH-R). No change in GnRH-R occurs during the time of LH release (15-60 min) or up until 4 h post-GnRH. Between 4 and 12 h the...

journal_title：Molecular and cellular endocrinology

authors： Detta A,Naik SI,Charlton HM,Young LS,Clayton RN

更新日期：1984-09-01 00:00:00

abstract：:17 beta-Hydroxysteroid dehydrogenase type 1 (17-HSD type 1) is a steroidogenic enzyme catalyzing reversible interconversion of estradiol and estrone. 17-HSD type 1 is actively expressed in human placenta. We characterized 17-HSD type 1 expression and its regulation by basic fibroblast growth factor (bFGF) in JAR, JEG-...

journal_title：Molecular and cellular endocrinology

authors： Lewintre EJ,Orava M,Peltoketo H,Vihko R

更新日期：1994-08-01 00:00:00

abstract：:In this study we have investigated the protein phosphorylation pattern in the membrane fraction prepared from bovine luteal cells. The phosphorylation reaction was carried out in vitro, under defined conditions, using either [gamma-32P]ATP or [gamma-35S]ATP as the phosphate donor. The results obtained show that [gamma...

journal_title：Molecular and cellular endocrinology

authors： Budnik LT,Mukhopadhyay AK

更新日期：1990-03-05 00:00:00

abstract：:The present experiment used cultured mouse cumulus cell-enclosed oocytes (CEOs) and denuded oocytes (DOs) to study the function of nitric oxide (NO) in mouse oocyte meiotic maturation. Either positive or negative actions of NO on meiotic maturation has been observed when CEOs or DOs were cultured for 24 h in a medium ...

journal_title：Molecular and cellular endocrinology

authors： Bu S,Xia G,Tao Y,Lei L,Zhou B

更新日期：2003-09-30 00:00:00

abstract：:The time- and dose-dependent effects of bovine activin A and bovine follicle stimulating hormone (FSH) suppressing protein (FSP) or follistatin on basal and FSH-induced steroidogenesis and inhibin production were studied in granulosa cells from immature, diethylstilbestrol (DES)-treated rats. In the presence of rat FS...

journal_title：Molecular and cellular endocrinology

authors： Xiao S,Findlay JK,Robertson DM

更新日期：1990-02-12 00:00:00

abstract：:Using a combination of in vitro assays we have evaluated whether DDT metabolites can interact with the progesterone receptor pathway in yeast expressing human progesterone receptor (hPR) and in T47D human breast cancer cells which express endogenous hPR. In transactivation assays using both yeast and T47D cells, o,p'-...

journal_title：Molecular and cellular endocrinology

authors： Klotz DM,Ladlie BL,Vonier PM,McLachlan JA,Arnold SF

更新日期：1997-04-25 00:00:00

abstract：:The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were c...

journal_title：Molecular and cellular endocrinology

authors： Macedo TJS,Santos JMS,Bezerra MÉS,Menezes VG,Gouveia BB,Barbosa LMR,Lins TLBG,Monte APO,Barberino RS,Batista AM,Barros VRP,Wischral A,Queiroz MAA,Araújo GGL,Matos MHT

更新日期：2019-09-15 00:00:00

abstract：:Some pituitary hormones are expressed in leukocytes and are thought to play a role in the regulation of leukocyte function. We studied the expression of the mRNA for the beta-chains of luteinising hormone (LHbeta) and chorionic gonadotropin (CGbeta) and their translation into protein in various leukocyte subsets. Mono...

journal_title：Molecular and cellular endocrinology

authors： Hotakainen PK,Serlachius EM,Lintula SI,Alfthan HV,Schröder JP,Stenman UE

更新日期：2000-04-25 00:00:00

abstract：:Successful natural reproduction normally requires vigorously motile spermatozoa. Using a signal peptide trapping strategy, we identified two new genes, a putative sperm Na+/H+ exchanger (sNHE) and the putative cation channel CatSper2, with unique and essential roles in sperm motility. Disruption of the sNHE or CatSper...

journal_title：Molecular and cellular endocrinology

authors： Quill TA,Wang D,Garbers DL

更新日期：2006-05-16 00:00:00

abstract：:Ultraviolet radiation B stimulates both the production of vitamin D3 in the skin and the activation of the skin analog of the hypothalamic-pituitary-adrenal axis (HPA) as well as the central HPA. Since the role of vitamin D3 in the regulation of the HPA is largely unknown, we investigated the impact of 1,25(OH)2D3 and...

journal_title：Molecular and cellular endocrinology

authors： Wierzbicka JM,Żmijewski MA,Piotrowska A,Nedoszytko B,Lange M,Tuckey RC,Slominski AT

更新日期：2016-12-05 00:00:00

abstract：:Adenylate cyclase activity and its modulation by guanine nucleotides and isoproterenol were assessed in adipocyte membranes of mice with mutations causing different genetic obesity syndromes. The object was to determine whether the defect in inhibitory modulation observed in the obese (ob/ob) mouse was also present in...

journal_title：Molecular and cellular endocrinology

authors： Bégin-Heick N,Coleman DL

更新日期：1988-10-01 00:00:00

abstract：:The production of glucocorticoids and aldosterone in the adrenal cortex is regulated at multiple levels. Biosynthesis of these hormones is initiated when cholesterol, the substrate, enters the inner mitochondrial membrane for conversion to pregnenolone. Unlike most metabolic pathways, the biosynthesis of adrenocortica...

journal_title：Molecular and cellular endocrinology

authors： Sewer MB,Li D

更新日期：2013-05-22 00:00:00

abstract：:The FOXO1 transcription factor is important for multiple aspects of reproductive function. We previously reported that FOXO1 functions as a repressor of gonadotropin hormone synthesis, but how FOXO1 is regulated in pituitary gonadotropes is unknown. The growth factors, insulin and insulin-like growth factor I (IGF1), ...

journal_title：Molecular and cellular endocrinology

authors： Skarra DV,Thackray VG

更新日期：2015-04-15 00:00:00