Abstract:
:Metazoan development depends on tightly regulated gene expression programs that instruct progenitor cells to adopt specialized fates. Recent work found that posttranslational modifications, such as monoubiquitylation, can determine cell fate also independently of effects on transcription, yet how monoubiquitylation is implemented during development is poorly understood. Here, we have identified a regulatory circuit that controls monoubiquitylation-dependent neural crest specification by the E3 ligase CUL3 and its substrate adaptor KBTBD8. We found that CUL3KBTBD8 monoubiquitylates its essential targets only after these have been phosphorylated in multiple motifs by CK2, a kinase whose levels gradually increase during embryogenesis. Its dependency on multisite phosphorylation allows CUL3KBTBD8 to convert the slow rise in embryonic CK2 into decisive recognition of ubiquitylation substrates, which in turn is essential for neural crest specification. We conclude that multisite dependency of an E3 ligase provides a powerful mechanism for switch-like cell fate transitions controlled by monoubiquitylation.
journal_name
Elifejournal_title
eLifeauthors
Werner A,Baur R,Teerikorpi N,Kaya DU,Rape Mdoi
10.7554/eLife.35407subject
Has Abstractpub_date
2018-07-12 00:00:00issn
2050-084Xpii
35407journal_volume
7pub_type
杂志文章相关文献
eLife文献大全abstract::When a human catches a ball, they estimate future target location based on the current trajectory. How animals, small and large, encode such predictive processes at the single neuron level is unknown. Here we describe small target-selective neurons in predatory dragonflies that exhibit localized enhanced sensitivity f...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26478
更新日期:2017-07-25 00:00:00
abstract::Two distinct mechanisms for primordial germ cell (PGC) specification are observed within Bilatera: early determination by maternal factors or late induction by zygotic cues. Here we investigate the molecular basis for PGC specification in Nematostella, a representative pre-bilaterian animal where PGCs arise as paired ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54573
更新日期:2020-09-24 00:00:00
abstract::The calcium release-activated calcium channel Orai regulates Ca2+ entry into non-excitable cells and is required for proper immune function. While the channel typically opens following Ca2+ release from the endoplasmic reticulum, certain pathologic mutations render the channel constitutively open. Previously, using on...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.62772
更新日期:2020-11-30 00:00:00
abstract::β-Propellers arise through the amplification of a supersecondary structure element called a blade. This process produces toroids of between four and twelve repeats, which are almost always arranged sequentially in a single polypeptide chain. We found that new propellers evolve continuously by amplification from single...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49853
更新日期:2019-10-15 00:00:00
abstract::The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12470
更新日期:2016-03-01 00:00:00
abstract::A temperature-sensitive receptor prevents mosquitoes from being attracted to targets that are hotter than a potential host. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.12838
更新日期:2015-12-15 00:00:00
abstract::The progenitor cells of the developing liver can differentiate toward both hepatocyte and biliary cell fates. In addition to the established roles of TGFβ and Notch signaling in this fate specification process, there is increasing evidence that liver progenitors are sensitive to mechanical cues. Here, we utilized micr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.38536
更新日期:2018-12-27 00:00:00
abstract::Microbial fitness screens are a key technique in functional genomics. We present an all-in-one solution, pyphe, for automating and improving data analysis pipelines associated with large-scale fitness screens, including image acquisition and quantification, data normalisation, and statistical analysis. Pyphe is versat...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55160
更新日期:2020-06-16 00:00:00
abstract::Transcription at individual genes in single cells is often pulsatile and stochastic. A key question emerges regarding how this behaviour contributes to tissue phenotype, but it has been a challenge to quantitatively analyse this in living cells over time, as opposed to studying snap-shots of gene expression state. We ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.08494
更新日期:2016-02-01 00:00:00
abstract::The pressure for every research article to tell a clear story often leads researchers in the life sciences to exclude experiments that 'did not work' when they write up their results. However, this practice can lead to reporting bias if the decisions about which experiments to exclude are taken after data have been co...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56626
更新日期:2020-05-22 00:00:00
abstract::In all living organisms, ribosomes translating membrane proteins are targeted to membrane translocons early in translation, by the ubiquitous signal recognition particle (SRP) system. In eukaryotes, the SRP Alu domain arrests translation elongation of membrane proteins until targeting is complete. Curiously, however, ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03440
更新日期:2014-08-18 00:00:00
abstract::Yes. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40018
更新日期:2018-07-23 00:00:00
abstract::The straight-tusked elephants Palaeoloxodon spp. were widespread across Eurasia during the Pleistocene. Phylogenetic reconstructions using morphological traits have grouped them with Asian elephants (Elephas maximus), and many paleontologists place Palaeoloxodon within Elephas. Here, we report the recovery of full mit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25413
更新日期:2017-06-06 00:00:00
abstract::Precise and efficient manipulation of genes is crucial for understanding the molecular mechanisms that govern human hematopoiesis and for developing novel therapies for diseases of the blood and immune system. Current methods do not enable precise engineering of complex genotypes that can be easily tracked in a mixed ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27873
更新日期:2017-09-28 00:00:00
abstract::Although several sleep-regulating neuronal populations have been identified, little is known about how they interact with each other to control sleep/wake states. We previously identified neuropeptide VF (NPVF) and the hypothalamic neurons that produce it as a sleep-promoting system (Lee et al., 2017). Here we show us...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54491
更新日期:2020-12-18 00:00:00
abstract::The expansion of bacterial swarms and the spreading of biofilms can be described by a unified biophysical theory that involves both active and passive processes. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47019
更新日期:2019-04-30 00:00:00
abstract::A universal and unquestioned characteristic of eukaryotic cells is that the genome is divided into multiple chromosomes and encapsulated in a single nucleus. However, the underlying mechanism to ensure such a configuration is unknown. Here, we provide evidence that pericentromeric satellite DNA, which is often regarde...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34122
更新日期:2018-03-26 00:00:00
abstract::Calcineurin is responsible for mediating a wide variety of cellular processes in response to dynamic calcium (Ca(2+)) signals, yet the precise mechanisms involved in the spatiotemporal control of calcineurin signaling are poorly understood. Here, we use genetically encoded fluorescent biosensors to directly probe the ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03765
更新日期:2014-07-23 00:00:00
abstract::Telomeres are nucleoprotein complexes at the ends of chromosomes and are indispensable for the protection and lengthening of terminal DNA. Despite the evolutionarily conserved roles of telomeres, the telomeric double-strand DNA (dsDNA)-binding proteins have evolved rapidly. Here, we identified double-strand telomeric ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.64104
更新日期:2021-01-21 00:00:00
abstract::Microtubule dynamics facilitate neurite growth and establish morphology, but the role of minus-end binding proteins in these processes is largely unexplored. CAMSAP homologs associate with microtubule minus-ends, and are important for the stability of epithelial cell adhesions. In this study, we report morphological d...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01637
更新日期:2014-02-25 00:00:00
abstract::Patterns of spatial positioning of individuals within microbial communities are often critical to community function. However, understanding patterning in natural communities is hampered by the multitude of cell-cell and cell-environment interactions as well as environmental variability. Here, through simulations and ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00230
更新日期:2013-01-22 00:00:00
abstract::Disturbance from whale-watching can cause significant behavioural changes with fitness consequences for targeted whale populations. However, the sensory stimuli triggering these responses are unknown, preventing effective mitigation. Here, we test the hypothesis that vessel noise level is a driver of disturbance, usin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56760
更新日期:2020-06-16 00:00:00
abstract::The precise pattern of motor neuron (MN) activation is essential for the execution of motor actions; however, the molecular mechanisms that give rise to specific patterns of MN activity are largely unknown. Phrenic MNs integrate multiple inputs to mediate inspiratory activity during breathing and are constrained to fi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52859
更新日期:2020-01-16 00:00:00
abstract::Improving the peer review process, overcoming the limitations of print journals and providing open access to the very best work in the life and biomedical sciences are three highlights of our first year. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01516
更新日期:2013-10-15 00:00:00
abstract::Temporal patterning of neural progenitors leads to the sequential production of diverse neurons. To understand how extrinsic cues influence intrinsic temporal programs, we studied Drosophila mushroom body progenitors (neuroblasts) that sequentially produce only three neuronal types: γ, then α'β', followed by αβ. Oppos...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58880
更新日期:2020-07-06 00:00:00
abstract::The canonical action of the p85α regulatory subunit of phosphatidylinositol 3-kinase (PI3K) is to associate with the p110α catalytic subunit to allow stimuli-dependent activation of the PI3K pathway. We elucidate a p110α-independent role of homodimerized p85α in the positive regulation of PTEN stability and activity. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.06866
更新日期:2015-07-29 00:00:00
abstract::The COVID-19 pandemic has resulted in school closures and distancing requirements that have disrupted both work and family life for many. Concerns exist that these disruptions caused by the pandemic may not have influenced men and women researchers equally. Many medical journals have published papers on the pandemic, ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58807
更新日期:2020-06-15 00:00:00
abstract::Modern neuroscience research often requires the coordination of multiple processes such as stimulus generation, real-time experimental control, as well as behavioral and neural measurements. The technical demands required to simultaneously manage these processes with high temporal fidelity is a barrier that limits the...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40231
更新日期:2019-02-07 00:00:00
abstract::Journals and other scientific organizations produce a diverse variety of plain-language summaries. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25411
更新日期:2017-03-15 00:00:00
abstract::Burkholderia thailandensis uses acyl-homoserine lactone-mediated quorum sensing systems to regulate hundreds of genes. Here we show that cell-cell contact-dependent type VI secretion (T6S) toxin-immunity systems are among those activated by quorum sensing in B. thailandensis. We also demonstrate that T6S is required t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14712
更新日期:2016-05-16 00:00:00