Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay.

Abstract:

AIM:A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. METHODS:The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping. RESULTS:The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48). CONCLUSION:Our prevalence data from Pakistan show that - as in other parts of the world - carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.

journal_name

Future Microbiol

journal_title

Future microbiology

authors

Braun SD,Jamil B,Syed MA,Abbasi SA,Weiß D,Slickers P,Monecke S,Engelmann I,Ehricht R

doi

10.2217/fmb-2018-0082

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

1225-1246

eissn

1746-0913

issn

1746-0921

journal_volume

13

pub_type

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