Ethylene modulates root cortical senescence in barley.

Abstract:

Background and Aims:Root cortical senescence (RCS) is a poorly understood phenomenon with implications for adaptation to edaphic stress. It was hypothesized that RCS in barley (Hordeum vulgare L.) is (1) accelerated by exogenous ethylene exposure; (2) accompanied by differential expression of ethylene synthesis and signalling genes; and (3) associated with differential expression of programmed cell death (PCD) genes. Methods:Gene expression of root segments from four barley genotypes with and without RCS was evaluated using quantitative real-time PCR (qRT-PCR). The progression of RCS was manipulated with root zone ethylene and ethylene inhibitor applications. Key Results:The results demonstrate that ethylene modulates RCS. Four genes related to ethylene synthesis and signalling were upregulated during RCS in optimal, low nitrogen and low phosphorus nutrient regimes. RCS was accelerated by root zone ethylene treatment, and this effect was reversed by an ethylene action inhibitor. Roots treated with exogenous ethylene had 35 and 46 % more cortical senescence compared with the control aeration treatment in seminal and nodal roots, respectively. RCS was correlated with expression of two genes related to programmed cell death (PCD). Conclusions:The development of RCS is similar to root cortical aerenchyma formation with respect to ethylene modulation of the PCD process.

journal_name

Ann Bot

journal_title

Annals of botany

authors

Schneider HM,Wojciechowski T,Postma JA,Brown KM,Lynch JP

doi

10.1093/aob/mcy059

subject

Has Abstract

pub_date

2018-06-28 00:00:00

pages

95-105

issue

1

eissn

0305-7364

issn

1095-8290

pii

4979863

journal_volume

122

pub_type

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