Abstract:
:Platelet-rich plasma (PRP) is used in therapy for bone tissue repair because an abundance of osteogenesis-related growth factors can be released from the concentrated platelets. However, its clinical use is limited because growth factors, temporally released from PRP, are degraded rapidly. This study aimed to incorporate PRP-derived growth factors into SF/PCL/PVA nanofibers by coaxial electrospinning to determine the release profiles of growth factors and how the presence of these growth factors enhances the osteogenic abilities of the nanofibers. Scaffolds containing different ratios of PRP and PVA were prepared and characterized. We then quantified the release of growth factors from the nanofibers over time, and evaluated the proliferation, migration and osteogenic differentiation of MSCs. The in vivo osteogenic capacity of the PRP-containing core-shell NFS was also evaluated by transplanting the PRP/MSCs/CS/β-TCP compounds into the skin on the back of nude mice and by treating cranial defects of C57BL/6 mice. The results of such treatments were analyzed by immunofluorescent staining, μ computed tomography (μCT), and histological observation. The results show that coaxial nanofibers with a PRP-5% PVA solution ratio of 7:1 contained a relatively high amount of PRP and exhibited a more uniform distribution of fiber diameters. The bioactivity of the scaffolds was enhanced due to the increased proliferation and migration of bone marrow mesenchymal stem cells (BMSCs). When the cells were inoculated and cultured on the PRP-loaded nanofibrous mats, the expression of collagen type II also increased. Furthermore, new bone formation was also promoted by PRP-NFS after 8 weeks of implantation. In conclusion, this study shows that the incorporation of PRP had positive effects on the bioactivity and osteogenic ability of coaxial nanofibrous mats. Such nanofibrous mats may prove beneficial in various applications of bone tissue engineering.
journal_name
Int J Pharmjournal_title
International journal of pharmaceuticsauthors
Cheng G,Ma X,Li J,Cheng Y,Cao Y,Wang Z,Shi X,Du Y,Deng H,Li Zdoi
10.1016/j.ijpharm.2018.06.020subject
Has Abstractpub_date
2018-08-25 00:00:00pages
656-666issue
1-2eissn
0378-5173issn
1873-3476pii
S0378-5173(18)30418-6journal_volume
547pub_type
杂志文章abstract::The poly(cystaminebis(acrylamide)-diaminohexane) (poly(CBA-DAH)) was designed previously as a bio-reducible efficient gene delivery carrier. However, the high weight ratio required to form the polyplexes between poly(CBA-DAH) with pDNA is still a problem that needs to be addressed. To solve this problem and increase t...
journal_title:International journal of pharmaceutics
pub_type: 杂志文章
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abstract::Three-dimensional printing (3DP) is a highly disruptive technology with the potential to change the way pharmaceuticals are designed, prescribed and produced. Owing to its low cost, diversity, portability and simplicity, fused deposition modeling (FDM) is well suited to a multitude of pharmaceutical applications in di...
journal_title:International journal of pharmaceutics
pub_type: 杂志文章,评审
doi:10.1016/j.ijpharm.2018.07.024
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2008.01.023
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2006.05.049
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journal_title:International journal of pharmaceutics
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doi:10.1016/j.ijpharm.2019.118560
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2019.118905
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abstract:AIMS AND OBJECTIVES:The aims and objectives of this article are to provide a background to the innovations that are made in over the counter market and to outline the time and costs involved in these activities. ...
journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2014.02.042
更新日期:2014-08-05 00:00:00
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2013.09.024
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2007.10.053
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2015.11.029
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pub_type: 杂志文章
doi:10.1016/j.ijpharm.2020.119938
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/s0378-5173(99)00170-2
更新日期:1999-08-05 00:00:00
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2014.07.049
更新日期:2014-10-01 00:00:00
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2015.08.062
更新日期:2015-10-15 00:00:00
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章,评审
doi:10.1016/j.ijpharm.2011.03.024
更新日期:2011-06-30 00:00:00
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2020.119694
更新日期:2020-09-25 00:00:00
abstract::The hemocompatibility of nanoparticles is of critical importance for their systemic administration as drug delivery systems. Formulations of lipid-core nanocapsules, stabilized with polysorbate 80-lecithin and uncoated or coated with chitosan (LNC and LNC-CS), were prepared and characterized by laser diffraction (D[4,...
journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2012.01.051
更新日期:2012-04-15 00:00:00
abstract::The epidural administration is used commonly in the treatment of pain. Nonsteroidal anti-inflammatory drugs, especially ibuprofen, would have potential in epidural use. Like many epidurally useful drugs it, however, has a short duration of action, which is a limiting factor. To improve epidural pain treatment, a long-...
journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/s0378-5173(00)00376-8
更新日期:2000-04-10 00:00:00
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2017.10.004
更新日期:2017-12-20 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.ijpharm.2020.119580
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2012.04.074
更新日期:2012-08-20 00:00:00
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2015.07.011
更新日期:2015-08-15 00:00:00
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/s0378-5173(00)00336-7
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2008.08.050
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pub_type: 杂志文章
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
doi:10.1016/j.ijpharm.2008.11.019
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journal_title:International journal of pharmaceutics
pub_type: 杂志文章
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