Abstract:
:Light represents the principal signal driving circadian clock entrainment. However, how light influences the evolution of the clock remains poorly understood. The cavefish Phreatichthys andruzzii represents a fascinating model to explore how evolution under extreme aphotic conditions shapes the circadian clock, since in this species the clock is unresponsive to light. We have previously demonstrated that loss-of-function mutations targeting non-visual opsins contribute in part to this blind clock phenotype. Here, we have compared orthologs of two core clock genes that play a key role in photic entrainment, cry1a and per2, in both zebrafish and P. andruzzii. We encountered aberrantly spliced variants for the P. andruzzii per2 transcript. The most abundant transcript encodes a truncated protein lacking the C-terminal Cry binding domain and incorporating an intronic, transposon-derived coding sequence. We demonstrate that the transposon insertion leads to a predominantly cytoplasmic localization of the cavefish Per2 protein in contrast to the zebrafish ortholog which is distributed in both the nucleus and cytoplasm. Thus, it seems that during evolution in complete darkness, the photic entrainment pathway of the circadian clock has been subject to mutation at multiple levels, extending from opsin photoreceptors to nuclear effectors.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ceinos RM,Frigato E,Pagano C,Fröhlich N,Negrini P,Cavallari N,Vallone D,Fuselli S,Bertolucci C,Foulkes NSdoi
10.1038/s41598-018-27080-2subject
Has Abstractpub_date
2018-06-08 00:00:00pages
8754issue
1issn
2045-2322pii
10.1038/s41598-018-27080-2journal_volume
8pub_type
杂志文章abstract::Most of the human genome is non-coding. However, some of the non-coding part is transcriptionally active. In humans, the tandemly repeated (TR) pericentromeric non-coding DNA-human satellites 2 and 3 (HS2, HS3)-are transcribed in somatic cells. These transcripts are also found in pre- and post-implantation embryos. Th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76628-8
更新日期:2020-11-12 00:00:00
abstract::This study aimed to assess the validity and functional relevance of a standardized procedure to assess lower limb muscle power by means of the 30-s sit-to-stand (STS) test when compared to leg extension power (LEP), traditional STS performance and handgrip strength. A total of 628 community-dwelling older subjects (60...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73395-4
更新日期:2020-10-01 00:00:00
abstract::Toughening of Fe-based amorphous coatings meanwhile maintaining a good corrosion resistance remains challenging. This work reports a novel approach to improve the toughness of a FeCrMoCBY amorphous coating through in-situ formation of amorphous carbon reinforcement without reducing the corrosion resistance. The Fe-bas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04504-z
更新日期:2017-06-22 00:00:00
abstract::Understanding past human settlement of inhospitable regions is one of the most intriguing puzzles in archaeological research, with implications for more sustainable use of marginal regions today. During the Byzantine period in the 4th century CE, large settlements were established in the arid region of the Negev Deser...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58360-5
更新日期:2020-01-30 00:00:00
abstract::Tumor oxygenation has been correlated with treatment outcome for radiotherapy. In this work, the dependence of tumor oxygenation on tumor vascularity and blood oxygenation was determined quantitatively in a 4D stochastic computational model of head and neck squamous cell carcinoma (HNSCC) tumor growth and angiogenesis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11444-1
更新日期:2017-09-08 00:00:00
abstract::For accurate micro-scale quantification of a specific protein in biological fluids, immunoaffinity chromatography (IAC) and isoelectric focusing (IEF) were combined in a single fused-silica capillary. The inner wall of the capillary was coated with an anti-E-tag antibody at the inlet side to form an IAC column, and po...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39221
更新日期:2016-12-15 00:00:00
abstract::Long non-coding RNAs (lncRNAs) have been found to play important roles in various biological processes; however, many of their functions remain unclear. In this study, we present a novel approach to identify the lncRNA-associated protein-protein interaction (PPI) modules and ascertain their functions in human lung squ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35939
更新日期:2016-10-27 00:00:00
abstract::Siwei Jianghuang Decoction Powder (SWJH) documented originally in the Four Medical Tantras-Blue Glaze exhibited beneficial effects on diabetic nephropathy (DN) via combined synergistically action of multiple formula components including Curcumae longae Rhizoma, Berberidis dictyophyllae Cortex, Phyllanthi Fructus and T...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35148-2
更新日期:2018-11-12 00:00:00
abstract::As a noninvasive and "task-free" technique, resting-state functional magnetic resonance imaging (rs-fMRI) has been gradually applied to pre-surgical functional mapping. Independent component analysis (ICA)-based mapping has shown advantage, as no a priori information is required. We developed an automated method for i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14248-5
更新日期:2017-10-23 00:00:00
abstract::Numerous studies have been conducted to examine the molecular mechanism of Haemophilus parasuis resistance to antibiotic, but rarely to tildipirosin. In the current study, transcriptional profiling was applied to analyse the variation in gene expression of JS0135 and tildipirosin-resistant JS32. The growth curves show...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07972-5
更新日期:2017-08-08 00:00:00
abstract::In this study, the effects of different feeding frequencies on the growth and the expression of genes in the GH/IGF axis were assessed in juvenile Chinese sturgeon. The newly hatched Chinese sturgeons were bred for 38 days at three different feeding frequencies groups (feeding frequency of two times a day, TWD; three ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74120-x
更新日期:2020-10-15 00:00:00
abstract::Nanoporous electrodes have received great attention because of their unique electrochemical properties. Here, the electrocatalytic oxygen evolution reaction (OER) activities at porous Pt layers with pore dimensions in the microporous range were examined. The OER activity of the porous Pt layers in acidic media increas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15688-9
更新日期:2017-11-13 00:00:00
abstract::Mechanistic target of rapamycin (mTOR) is a serine/threonine protein kinase that mediates phosphoinositide-3-kinase (PI3K)/AKT signalling. This pathway is involved in a plethora of cellular functions including protein and lipid synthesis, cell migration, cell proliferation and apoptosis. In this study, we proposed to ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53141-1
更新日期:2019-11-15 00:00:00
abstract::In South American societies, domesticated camelids were of great cultural importance and subject to trade and translocation. South American camelids were even found on remote and hard to reach islands, emphasizing their importance to historic and pre-historic South American populations. Isla Mocha, a volcanic island 3...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38708
更新日期:2016-12-08 00:00:00
abstract::Receipt of broad-spectrum antibiotics enhances Candida albicans colonization of the GI tract, a risk factor for haematogenously-disseminated candidiasis. To understand how antibiotics influence C. albicans colonization, we treated mice orally with vancomycin or a combination of penicillin, streptomycin, and gentamicin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08131
更新日期:2015-02-03 00:00:00
abstract::Haber-Bosch nitrogen (N) has been increasingly used in industrial products, e.g., nylon, besides fertilizer. Massive numbers of species of industrial reactive N (Nr) have emerged and produced definite consequences but receive little notice. Based on a comprehensive inventory, we show that (1) the industrial N flux has...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02579
更新日期:2013-01-01 00:00:00
abstract::Both field- and landscape-scale factors can influence the predator communities of agricultural pests, but the relative importance and interactions between these scales are poorly understood. Focusing on spiders, an important taxon for providing biological control, we tested the influence of field- and landscape-scale ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71888-w
更新日期:2020-09-15 00:00:00
abstract::To attach reliably on various inclined rough surfaces, many insects have evolved both claws and adhesive pads on their feet. However, the interaction between these organs still remains unclear. Here we designed an artificial attachment device, which mimics the structure and function of claws and adhesive pads, and tes...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26219
更新日期:2016-05-20 00:00:00
abstract::The neuropathology of Parkinson's disease (PD) involves the frontal-subcortical circuit, an area responsible for processing affective theory of mind (ToM). Patients with PD are expected to experience deficits in the affective ToM. This study aims to investigate whether the ability to infer emotion in others is affecte...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33988-6
更新日期:2018-10-30 00:00:00
abstract::For isotropic fluids, classical nucleation theory predicts the nucleation rate, barrier height and critical droplet size by ac- counting for the competition between bulk energy and interfacial tension. The nucleation process in liquid crystals is less understood. We numerically investigate nucleation in monolayered ne...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40059
更新日期:2017-01-05 00:00:00
abstract::In this study, we analyzed mutants of Clostridium acetobutylicum, an organism used in a broad range of industrial processes related to biofuel production, to facilitate future studies of bioreactor and bioprocess design and scale-up, which are very important research projects for industrial microbiology applications. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39875
更新日期:2017-01-03 00:00:00
abstract::Up to now, full tunability of waveforms was possible only in electronics, up to radio-frequencies. Here we propose a new concept of producing few-cycle terahertz (THz) pulses with widely tunable waveforms. It is based on control of the phase delay between different parts of the THz wavefront using linear diffractive o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43852-w
更新日期:2019-05-15 00:00:00
abstract::The dissolution of ferrihydrite induced by low molar mass (LMM) organics is an important process that provides bioavailable iron for organisms. Here, ATR-FTIR analysis was combined with characterization of ferrihydrite nanoparticles and kinetic modeling to investigate the roles of different oxalate surface complex spe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20401-5
更新日期:2018-02-01 00:00:00
abstract::Dislocations in crystals are linear crystallographic defects, which move in lattice when crystals are plastically deformed. Motion of a partial dislocation may remove or create stacking fault characterized with a partial of a lattice translation vector. Here we report that motion of partial dislocations inside an inte...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01039
更新日期:2013-01-01 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-31948-8
更新日期:2018-09-12 00:00:00
abstract::Stable isotope labeling is a standard technique for tracing material transfer in molecular, ecological and biogeochemical studies. The main assumption in this approach is that the enrichment with a heavy isotope has no effect on the organism metabolism and growth, which is not consistent with current theoretical and e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44181
更新日期:2017-03-10 00:00:00
abstract::In 2018, the World Health Organization identified the Zika virus (ZIKV) as a pathogen that should be prioritized for public health research due to its epidemic potential. In this study, whole-genome sequencing (WGS) of travel-acquired ZIKV infections was used to examine the limitations of phylogenetic analysis. WGS an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52613-8
更新日期:2019-11-11 00:00:00
abstract::The effect on post-operative outcomes after coronary artery bypass graft (CABG) surgery is not clear. Among 17,812 patients who underwent CABG during October 1,2006-September 28,2012 in any Department of US Veterans Affairs (VA) hospital, we identified 5,968 with available preoperative urine albumin-creatinine ratio (...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16458
更新日期:2015-11-09 00:00:00
abstract::Embryonic cryopreservation has a relatively low survival rate because of cytoskeletal damage. However, molecular anti-freezing mechanisms have been largely unexplored. This study investigated the significance of RhoA, involved in embryonic development, and the Rho/RhoA-associated kinase (ROCK) signalling pathway in cr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07066-2
更新日期:2017-07-27 00:00:00
abstract::Pain is a highly unpleasant sensory and emotional experience, and no objective diagnosis test exists to assess it. In clinical practice there are two main methods for the estimation of pain, a patient's self-report and clinical judgement. However, these methods are highly subjective and the need of biomarkers to measu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42098-w
更新日期:2019-04-04 00:00:00