Abstract:
:The onset, course, and regression of the biochemical and structural alterations associated with pancreatitis induced by various doses of caerulein were studied in the mouse. In addition, the protective effect of secretin was compared with that of the cholecystokinin-receptor antagonists proglumide and benzotript. Subcutaneous or intraperitoneal injections of caerulein induced increases in serum amylase concentration and pancreatic weight and histologic evidence of acute pancreatitis, all effects being dose-related. Cytoplasmic vacuoles were the earliest histologic alterations. As the pancreatitis progressed these vacuoles increased to an enormous size. Interstitial inflammation and acinar cell necrosis were prominent after 6 h, reached a maximum after 12 h, and mostly disappeared after 4 days. During the course of pancreatitis approximately 40% of the acinar cells showed signs of severe degeneration or necrosis at the most effective doses of caerulein. Electron microscopy showed both intact and degenerating granules inside the vacuoles. Signs of basolateral exocytosis of zymogen granules were not observed. During the regression of pancreatitis, focal atrophy was a remarkable histologic finding. Repetitive initiation of pancreatitis (six courses of caerulein injections over 5 wk) produced marked focal atrophy and early fibrosis. High doses of proglumide or benzotript markedly ameliorated both the biochemical and structural alterations induced by caerulein. Secretin, even at very high doses, had only minor protective effects. This study presents a model of acute necrotizing pancreatitis in which the severity of the induced pancreatitis ranges dose-dependently from mild interstitial inflammation to severe necrosis. The ultrastructural alterations described herein support the hypothesis that the trigger mechanism of acute pancreatitis appears to be a primary intracellular event rather than an interstitial event that secondarily damages the acinar cells.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Niederau C,Ferrell LD,Grendell JHdoi
10.1016/s0016-5085(85)80079-2subject
Has Abstractpub_date
1985-05-01 00:00:00pages
1192-204issue
5 Pt 1eissn
0016-5085issn
1528-0012pii
S0016508585001457journal_volume
88pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND & AIMS:Corticotropin-releasing factor (CRF) signaling pathways play a key role in the stress response through the activation of CRF(1) and CRF(2) receptors. We investigated the CRF receptor subtypes involved in gastric postoperative ileus. METHODS:Adult male mice (C57BL/6, CRF(1)-deficient, and wild-type), ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(03)01069-2
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND & AIMS:The mechanism by which gastric mucosa becomes more resistant to damage by repeated aspirin administration is not known. Transforming growth factor alpha (TGF-alpha) and epidermal growth factor (EGF) prevent drug-induced gastric injury. The aim of this study was to determine whether gastroduodenal tiss...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1996.v110.pm8613050
更新日期:1996-05-01 00:00:00
abstract::Using in vivo and in vitro techniques, the mechanism by which intestinal mucosa obtains glutamine from luminal oligopeptides was investigated in humans. The rate of hydrolysis by mucosal brush border membrane was more than threefold greater for alanylglutamine than for glycylglutamine. Despite this difference, rates o...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)91088-l
更新日期:1992-07-01 00:00:00
abstract:BACKGROUND & AIMS:Krüppel-like factor 5 (KLF5) is a transcription factor that promotes proliferation, is highly expressed in dividing crypt cells of the gastrointestinal epithelium, and is induced by various stress stimuli. We sought to determine the role of KLF5 in colonic inflammation and recovery by studying mice wi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2010.10.061
更新日期:2011-02-01 00:00:00
abstract::The role of central (supraspinal and spinal) and peripheral alpha-adrenoceptors in the regulation of gastrointestinal propulsion in the mouse was studied using clonidine, an alpha 2-adrenoceptor agonist. Clonidine produced a dose-dependent inhibition of propulsion when given intracerebroventricularly, intrathecally, o...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(88)90360-5
更新日期:1988-11-01 00:00:00
abstract:BACKGROUND & AIMS:Patients with colorectal tumors with microsatellite instability (MSI) have better prognoses than patients with tumors without MSI, but have a poor response to 5-fluorouracil–based chemotherapy. A dominant-negative form of heat shock protein (HSP)110 (HSP110DE9) expressed by cancer cells with MSI, via ...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.1053/j.gastro.2013.10.054
更新日期:2014-02-01 00:00:00
abstract::The effect of aging on gastric and duodenal mucosal prostaglandins has not been reported. Gastric and duodenal mucosal prostaglandin concentrations were measured in 46 healthy adults, 35 of whom were relatively young (21-40 years old) and 11 of whom were considerably more elderly (aged 52-72 years). Mucosal biopsy spe...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract:BACKGROUND & AIMS:Few studies, even those with cohort designs, test the mediating effects of infant gut microbes and metabolites on the onset of disease. We undertook such a study. METHODS:Using structural equation modeling path analysis, we tested directional relationships between first pregnancy, birth mode, prolong...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2020.08.053
更新日期:2021-01-01 00:00:00
abstract:BACKGROUND & AIMS:Administration of secretin improves noninvasive imaging of the pancreatic duct with magnetic resonance cholangiopancreatography (MRCP). We performed a large prospective study to investigate whether synthetic human secretin (RG1068)-stimulated MRCP detects pancreatic duct abnormalities with higher leve...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2014.05.035
更新日期:2014-09-01 00:00:00
abstract:BACKGROUND & AIMS:Alcoholic hepatitis (AH) is characterized by hepatocellular damage, inflammation, and fibrosis. We performed a prospective study to associate hepatic expression of the CXC subfamily of chemokines with histology findings and prognosis of patients with AH. METHODS:Liver biopsy samples from 105 patients...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.01.056
更新日期:2009-05-01 00:00:00
abstract::We investigated the ability of isolated human colonic epithelial cells to express the common acute lymphoblastic leukemia antigen (CALLA), the transferrin receptor, and the 4F2 antigen in response to different types of stimuli. The expression of these markers was assessed by immunofluorescence using monoclonal antibod...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)90387-9
更新日期:1989-12-01 00:00:00
abstract:BACKGROUND & AIMS:Reduced generation of all-trans retinoic acid (RA) by CD103(+) intestinal dendritic cells (DCs) is linked to intestinal inflammation in mice. However, the role of RA in intestinal inflammation in humans is unclear. We investigated which antigen-presenting cells (APCs) produce RA in the human intestine...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2014.01.057
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND & AIMS:The effects of aging on pancreatic acinar cell proliferation have not been clearly defined. Phosphatidylinositol 3-kinase (PI3K)-mediated phosphorylation of Akt is a critical step for proliferation of various cell types and insulin secretion from pancreatic endocrine cells; however, its role in acinar...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.03.016
更新日期:2005-05-01 00:00:00
abstract:BACKGROUND/AIMS:Staphylococcal enterotoxin B (SEB) acts as a superantigen binding to class II major histocompatibility complex proteins, and this complex stimulates T cells. The aim of this study was to investigate the pathogenic effects of SEB on hepatic injury and lethal shock in mice. METHODS:SEB was administered t...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(94)90604-1
更新日期:1994-02-01 00:00:00
abstract::Basal lower esophageal sphincter (LES) pressure was measured in 6 patients with the Zollinger-Ellison syndrome (ZES) and hypergastrinemia, and in 6 patients with duodenal ulcer and normogastrinemia. Basal LES pressure in ZES patients, 17.0 +/- 3.7 (mean +/- SE) mm Hg was similar to basal LES pressure in duodenal ulcer...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
abstract:BACKGROUND & AIMS:Regulation of blood flow and oxygen supply are important pathogenetic factors in alcoholic liver disease. Because nitric oxide may have an important role, its effects on alcoholic liver injury were investigated. METHODS:Rats were fed ethanol intragastrically with either saturated fat or corn oil. Spo...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(95)90400-x
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND & AIMS:The early detection of colorectal cancer is desired because this cancer can be cured surgically if diagnosed early. The purpose of the present study was to determine the feasibility of a new methodology for isolating colonocytes from naturally evacuated feces, followed by cytology or molecular biology...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.10.007
更新日期:2005-12-01 00:00:00
abstract:BACKGROUND & AIMS:Recent studies indicate that kringle 1-5 has a potent and specific antiangiogenic activity. Here, we investigated the antitumor effect of kringle 1-5 gene transfer on hepatocellular carcinoma in mice. METHODS:The inhibitory effect of kringle 1-5 protein on proliferation of bovine capillary endothelia...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2006.02.020
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND & AIMS:Although long-term results of endoscopic sphincterotomy (ES) have been poorly estimated, extended indications of ES have been proposed, especially in young patients. The aim of this study was to assess late biliary complications of ES. METHODS:Between 1981 and 1986, 169 patients younger than age 70 (...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1996.v110.pm8608900
更新日期:1996-03-01 00:00:00
abstract::Ethanol (10 or 15% in the drinking water) was found to increase the amount of collagen in the livers of rats receiving a cirrhogenic diet. This effect of ethanol was most striking in the animals receiving 10% ethanol. Ethanol lowered the amount of labeled proline incorporated into hydroxyproline and, therefore, into c...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract:BACKGROUND & AIMS:Despite high morbidity and mortality of alcoholic liver disease worldwide, the molecular mechanisms underlying alcohol-induced liver cell death are not fully understood. Transglutaminase 2 (TG2) is a cross-linking enzyme implicated in apoptosis. TG2 levels and activity are increased in association wit...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.01.007
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND & AIMS:The activation of Wnt/beta-catenin signaling causes the development of gastric and colon cancers. Sox17 represses Wnt/beta-catenin signaling and is down-regulated in colon cancer. This study was designed to elucidate the role of Sox17 during the course of gastrointestinal tumorigenesis. METHODS:Sox17...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.06.041
更新日期:2009-10-01 00:00:00
abstract::Esophageal ligation in the pylorus-ligated rat significantly inhibited volume, titratable acidity, and titratable acid output and reduced the incidence of ulcers, perforations, and death of 18-hr pylorus-ligated rats. Draining the saliva outside the body of the rat by esophageal cannulation produced a significant incr...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1966-06-01 00:00:00
abstract::Superficially invasive squamous cell carcinoma of the esophagus, defined as carcinoma with invasion limited to the mucosa or submucosa regardless of the presence of lymph node metastasis, is being increasingly recognized. We found 7 cases (13%) of this entity among 54 cases of esophageal squamous cancer resected betwe...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(86)90201-5
更新日期:1986-12-01 00:00:00
abstract::Wilson's disease may present with severe acute hepatocellular failure. The only effective treatment for fulminant Wilson's disease is liver transplantation, which may lead to reversal of the underlying disease. Some patients with cirrhosis who are too ill to undergo orthotopic liver transplantation have been treated w...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(90)90497-o
更新日期:1990-12-01 00:00:00
abstract:BACKGROUND & AIMS:Genetic factors are believed to affect risk for irritable bowel syndrome (IBS), but there have been no sufficiently powered and adequately sized studies. To identify DNA variants associated with IBS risk, we performed a genome-wide association study (GWAS) of the large UK Biobank population-based coho...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2018.03.064
更新日期:2018-07-01 00:00:00
abstract::The relationship between renal tubular acidosis (RTA) and copper metabolism has been investigated in a group of 18 patients with primary biliary cirrhosis. RTA, considered when urinary pH remained above 5.4 after an oral load of ammonium chloride of 0.1 g/kg body wt, was found in 6 patients (33%). Plasma copper concen...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1981-04-01 00:00:00
abstract:BACKGROUND & AIMS:Hepatitis E virus (HEV) infection can cause chronic hepatitis in recipients of solid organ transplants. However, the factors that contribute to chronic infection and the outcomes of these patients are incompletely understood. We performed a retrospective analysis of data from 17 centers from Europe an...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.1053/j.gastro.2011.02.050
更新日期:2011-05-01 00:00:00
abstract:BACKGROUND & AIMS:Because current therapies for chronic hepatitis C virus (HCV) infections are suboptimal and associated with severe side effects, novel treatment options are needed. A small animal model has recently been developed to study HCV infections. To examine the usefulness of this human liver-urokinase-type pl...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.07.007
更新日期:2007-10-01 00:00:00
abstract::Rotaviruses cause life-threatening gastroenteritis in children worldwide; the enormous disease burden has focused efforts to develop vaccines and led to the discovery of novel mechanisms of gastrointestinal virus pathogenesis and host responses to infection. Two live-attenuated vaccines for gastroenteritis (Rotateq [M...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2009.02.076
更新日期:2009-05-01 00:00:00