Abstract:
:P815, a murine mastocytoma cell line, possesses beta-adrenergic binding sites as assessed by using [3H]dihydroalprenolol (antagonist) and [3H]hydroxybenzylisoproterenol (agonist). The number of binding sites per cell was 29 000 for the agonist and 75 000 for the antagonist, as determined by direct binding assays and inhibition experiments on intact cells. On membrane preparations from the same cells, binding of alprenolol was only displaceable by antagonists, while stereospecific binding of hydroxybenzylisoproterenol was only displaceable by agonists. The P815 membranes also possessed an adenylate cyclase stimulated by Gpp(NH)p and NaF but not by 1-isoproterenol. The intracellular cAMP level of intact cells was not modulated by 1-isoproterenol or by 1-epinephrine, but was increased by forskolin. These results suggest that the beta-adrenergic receptor of P815 mastocytoma cells is non-functional. This may explain the failure of agonists to stimulate adenylate cyclase activity in these cells.
journal_name
Eur J Pharmacoljournal_title
European journal of pharmacologyauthors
Durieu-Trautmann O,Delavier-Klutchko C,Hoebeke J,Strosberg ADdoi
10.1016/0014-2999(85)90717-4subject
Has Abstractpub_date
1985-01-22 00:00:00pages
133-41issue
2eissn
0014-2999issn
1879-0712pii
0014-2999(85)90717-4journal_volume
108pub_type
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