Video-rate volumetric neuronal imaging using 3D targeted illumination.


:Fast volumetric microscopy is required to monitor large-scale neural ensembles with high spatio-temporal resolution. Widefield fluorescence microscopy can image large 2D fields of view at high resolution and speed while remaining simple and costeffective. A focal sweep add-on can further extend the capacity of widefield microscopy by enabling extended-depth-of-field (EDOF) imaging, but suffers from an inability to reject out-of-focus fluorescence background. Here, by using a digital micromirror device to target only in-focus sample features, we perform EDOF imaging with greatly enhanced contrast and signal-to-noise ratio, while reducing the light dosage delivered to the sample. Image quality is further improved by the application of a robust deconvolution algorithm. We demonstrate the advantages of our technique for in vivo calcium imaging in the mouse brain.


Sci Rep


Scientific reports


Xiao S,Tseng HA,Gritton H,Han X,Mertz J




Has Abstract


2018-05-21 00:00:00












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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Morand A,Tall ML,Kuete Yimagou E,Ngom II,Lo CI,Cornu F,Tsimaratos M,Lagier JC,Levasseur A,Raoult D,Fournier PE

    更新日期:2021-01-29 00:00:00

  • Taxonomic and functional diversity increase the aesthetic value of coralligenous reefs.

    abstract::The aesthetic value of landscapes contributes to human well-being. However, studies which have investigated the link between biodiversity and ecosystem services have not taken aesthetic value into account. In this study we evaluated how the aesthetics of coralligenous reefs, a key marine ecosystem in the Mediterranean...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Tribot AS,Mouquet N,Villéger S,Raymond M,Hoff F,Boissery P,Holon F,Deter J

    更新日期:2016-09-28 00:00:00

  • High-energy, Long-cycle-life Secondary Battery with Electrochemically Pre-doped Silicon Anode.

    abstract::Electrochemical pre-doping of a silicon electrode was investigated to create a new class of rechargeable battery with higher energy density. The electrochemical reaction during pre-doping formed a high-quality solid electrolyte interface (SEI) on the surface of silicon particles, which improved the charge and discharg...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Wang Y,Satoh M,Arao M,Matsumoto M,Imai H,Nishihara H

    更新日期:2020-02-21 00:00:00

  • Human GV oocytes generated by mitotically active germ cells obtained from follicular aspirates.

    abstract::Human female germline stem cells (FGSCs) have opened new opportunities for understanding human oogenesis, delaying menopause, treating infertility, and providing a new strategy for preserving fertility. However, the shortage of adult human ovaries tissues available impedes their future investigations and clinical appl...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Ding X,Liu G,Xu B,Wu C,Hui N,Ni X,Wang J,Du M,Teng X,Wu J

    更新日期:2016-06-30 00:00:00

  • Co-expression of BirA with biotin bait achieves in vivo biotinylation of overexpressed stable N-glycosylated sRAGE in transgenic silkworms.

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Kumano-Kuramochi M,Tatematsu KI,Ohnishi-Kameyama M,Maeda-Yamamoto M,Kobori T,Sezutsu H,Machida S

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  • Lufenuron induces reproductive toxicity and genotoxic effects in pregnant albino rats and their fetuses.

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Basal WT,Ahmed ART,Mahmoud AA,Omar AR

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Bode K,Nolte L,Kamin H,Desens M,Ulmann A,Bergmann GA,Betker P,Reitmeier J,Ripken T,Stern M,Meyer H,Bicker G

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  • Digitalization of the IOM: A comprehensive cadaveric study for obtaining three-dimensional models and morphological properties of the forearm's interosseous membrane.

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Carrillo F,Suter S,Casari FA,Sutter R,Nagy L,Snedeker JG,Fürnstahl P

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  • An absorption-free and Doppler-improved optical waveguide for diffractionless light propagation.

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Cui N,Gan Z,Zhang L

    更新日期:2017-10-27 00:00:00

  • Α 10-gigawatt attosecond source for non-linear XUV optics and XUV-pump-XUV-probe studies.

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Makos I,Orfanos I,Nayak A,Peschel J,Major B,Liontos I,Skantzakis E,Papadakis N,Kalpouzos C,Dumergue M,Kühn S,Varju K,Johnsson P,L'Huillier A,Tzallas P,Charalambidis D

    更新日期:2020-02-28 00:00:00

  • Removal of back-reflection noise at ultrathin imaging probes by the single-core illumination and wide-field detection.

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Yoon C,Kang M,Hong JH,Yang TD,Xing J,Yoo H,Choi Y,Choi W

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Guo Gao T,Yuan Xu Y,Jiang F,Zhen Li B,Shui Yang J,Tao Wang E,Li Yuan H

    更新日期:2015-06-08 00:00:00

  • Evaluation of the Structure of Myodural Bridges in an Equine Model of Ehlers-Danlos Syndromes.

    abstract::Myodural bridges have been described in various species as connective tissue structures "bridging" small cranio-cervical muscles to the dura. Myodural bridges are thought to stabilize the dural sac during head and neck movements and promote cerebrospinal fluid motion; however, their role in neurological diseases has n...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: McElroy A,Rashmir A,Manfredi J,Sledge D,Carr E,Stopa E,Klinge P

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Hussain A,AlAjmi MF,Rehman MT,Amir S,Husain FM,Alsalme A,Siddiqui MA,AlKhedhairy AA,Khan RA

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    journal_title:Scientific reports

    pub_type: 历史文章,杂志文章


    authors: Malekzadeh H,Lotfaliany M,Ostovar A,Hadaegh F,Azizi F,Yoosefi M,Farzadfar F,Khalili D

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Li N,Zhang J,Liao D,Yang L,Wang Y,Hou S

    更新日期:2017-02-16 00:00:00

  • Landscape limits gene flow and drives population structure in Agassiz's desert tortoise (Gopherus agassizii).

    abstract::Distance, environmental heterogeneity and local adaptation can strongly influence population structure and connectivity. Understanding how these factors shape the genomic landscape of threatened species is a major goal in conservation genomics and wildlife management. Herein, we use thousands (6,859) of single nucleot...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Sánchez-Ramírez S,Rico Y,Berry KH,Edwards T,Karl AE,Henen BT,Murphy RW

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  • Function of alanine racemase in the physiological activity and cariogenicity of Streptococcus mutans.

    abstract::The enzyme alanine racemase (Alr) has been a new target for the development of antibacterial drugs based on the involvement of D-Ala in bacterial cell wall biosynthesis. Our previous study noted that Alr is essential for the growth and interspecies competitiveness of S. mutans, the major causative organism of dental c...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Liu S,Wei Y,Zhou X,Zhang K,Peng X,Ren B,Chen V,Cheng L,Li M

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  • A Joint Modeling Approach for Childhood Meat, Fish and Egg Consumption and the Risk of Advanced Islet Autoimmunity.

    abstract::Several dietary factors have been suspected to play a role in the development of advanced islet autoimmunity (IA) and/or type 1 diabetes (T1D), but the evidence is fragmentary. A prospective population-based cohort of 6081 Finnish newborn infants with HLA-DQB1-conferred susceptibility to T1D was followed up to 15 year...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Syrjälä E,Nevalainen J,Peltonen J,Takkinen HM,Hakola L,Åkerlund M,Veijola R,Ilonen J,Toppari J,Knip M,Virtanen SM

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  • On-Surface Synthesis within a Porphyrin Nanoring Template.

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Judd CJ,Kondratuk DV,Anderson HL,Saywell A

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    abstract::A role of gut-brain axis emerges in the pathophysiology of anorexia nervosa and maintaining adapted physical activity during refeeding remains discussed. We aimed to assess gastrointestinal protein metabolism and investigate the contribution of physical activity during refeeding in C57BL/6 mice with activity-based ano...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Achamrah N,Nobis S,Breton J,Jésus P,Belmonte L,Maurer B,Legrand R,Bôle-Feysot C,do Rego JL,Goichon A,Rego JC,Déchelotte P,Fetissov SO,Claeyssens S,Coëffier M

    更新日期:2016-02-24 00:00:00

  • CREDO: Highly confident disease-relevant A-to-I RNA-editing discovery in breast cancer.

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Hwang W,Calza S,Silvestri M,Pawitan Y,Lee Y

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Koarashi J,Nishimura S,Atarashi-Andoh M,Muto K,Matsunaga T

    更新日期:2019-05-07 00:00:00

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Feng W,Zhu Y,Wu F,He Z,Zhang C,Giesy JP

    更新日期:2016-11-16 00:00:00

  • Improvement of osteogenesis by a uniform PCL coating on a magnesium screw for biodegradable applications.

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    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Kim YK,Lee KB,Kim SY,Jang YS,Kim JH,Lee MH

    更新日期:2018-09-05 00:00:00

  • Near-instant automatic access to visually presented words in the human neocortex: neuromagnetic evidence.

    abstract::Rapid and efficient processing of external information by the brain is vital to survival in a highly dynamic environment. The key channel humans use to exchange information is language, but the neural underpinnings of its processing are still not fully understood. We investigated the spatio-temporal dynamics of neural...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Shtyrov Y,MacGregor LJ

    更新日期:2016-05-24 00:00:00

  • Influence of OATP1B1 and BCRP polymorphisms on the pharmacokinetics and pharmacodynamics of rosuvastatin in elderly and young Korean subjects.

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    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章


    authors: Kim Y,Yoon S,Choi Y,Yoon SH,Cho JY,Jang IJ,Yu KS,Chung JY

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  • High Speed Computational Ghost Imaging via Spatial Sweeping.

    abstract::Computational ghost imaging (CGI) achieves single-pixel imaging by using a Spatial Light Modulator (SLM) to generate structured illuminations for spatially resolved information encoding. The imaging speed of CGI is limited by the modulation frequency of available SLMs, and sets back its practical applications. This pa...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Wang Y,Liu Y,Suo J,Situ G,Qiao C,Dai Q

    更新日期:2017-03-30 00:00:00

  • ADAMTS-7 is associated with a high-risk plaque phenotype in human atherosclerosis.

    abstract::Several large-scale genome-wide association studies have identified single-nucleotide polymorphisms in the genomic region of A Disintegrin And Metalloproteinase with ThromboSpondin type 1 repeats (ADAMTS)-7 and associations to coronary artery disease. Experimental studies have provided evidence for a functional role o...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章


    authors: Bengtsson E,Hultman K,Dunér P,Asciutto G,Almgren P,Orho-Melander M,Melander O,Nilsson J,Hultgårdh-Nilsson A,Gonçalves I

    更新日期:2017-06-16 00:00:00

  • Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.

    abstract::Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs). The PMEs are spatially regulated by pectin methylesterase inhibitors (PMEIs). In this study, 43 and 49 putative PME and P...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Zhang P,Wang H,Qin X,Chen K,Zhao J,Zhao Y,Yue B

    更新日期:2019-12-27 00:00:00