High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics.

Abstract:

:Optogenetics revolutionizes basic research in neuroscience and cell biology and bears potential for medical applications. We develop mutants leading to a unifying concept for the construction of various channelrhodopsins with fast closing kinetics. Due to different absorption maxima these channelrhodopsins allow fast neural photoactivation over the whole range of the visible spectrum. We focus our functional analysis on the fast-switching, red light-activated Chrimson variants, because red light has lower light scattering and marginal phototoxicity in tissues. We show paradigmatically for neurons of the cerebral cortex and the auditory nerve that the fast Chrimson mutants enable neural stimulation with firing frequencies of several hundred Hz. They drive spiking at high rates and temporal fidelity with low thresholds for stimulus intensity and duration. Optical cochlear implants restore auditory nerve activity in deaf mice. This demonstrates that the mutants facilitate neuroscience research and future medical applications such as hearing restoration.

journal_name

Nat Commun

journal_title

Nature communications

authors

Mager T,Lopez de la Morena D,Senn V,Schlotte J,D Errico A,Feldbauer K,Wrobel C,Jung S,Bodensiek K,Rankovic V,Browne L,Huet A,Jüttner J,Wood PG,Letzkus JJ,Moser T,Bamberg E

doi

10.1038/s41467-018-04146-3

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

1750

issue

1

issn

2041-1723

pii

10.1038/s41467-018-04146-3

journal_volume

9

pub_type

杂志文章
  • Quantifying cerebral contributions to pain beyond nociception.

    abstract::Cerebral processes contribute to pain beyond the level of nociceptive input and mediate psychological and behavioural influences. However, cerebral contributions beyond nociception are not yet well characterized, leading to a predominant focus on nociception when studying pain and developing interventions. Here we use...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms14211

    authors: Woo CW,Schmidt L,Krishnan A,Jepma M,Roy M,Lindquist MA,Atlas LY,Wager TD

    更新日期:2017-02-14 00:00:00

  • Atomically precise edge chlorination of nanographenes and its application in graphene nanoribbons.

    abstract::Chemical functionalization is one of the most powerful and widely used strategies to control the properties of nanomaterials, particularly in the field of graphene. However, the ill-defined structure of the present functionalized graphene inhibits atomically precise structural characterization and structure-correlated...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms3646

    authors: Tan YZ,Yang B,Parvez K,Narita A,Osella S,Beljonne D,Feng X,Müllen K

    更新日期:2013-01-01 00:00:00

  • Engineering and elucidation of the lipoinitiation process in nonribosomal peptide biosynthesis.

    abstract::Nonribosomal peptide synthetases containing starter condensation domains direct the biosynthesis of nonribosomal lipopeptides, which generally exhibit wide bioactivities. The acyl chain has strong impacts on bioactivity and toxicity, but the lack of an in-depth understanding of starter condensation domain-mediated lip...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-20548-8

    authors: Zhong L,Diao X,Zhang N,Li F,Zhou H,Chen H,Bai X,Ren X,Zhang Y,Wu D,Bian X

    更新日期:2021-01-12 00:00:00

  • Essential structural elements in tRNA(Pro) for EF-P-mediated alleviation of translation stalling.

    abstract::The ribosome stalls on translation of polyproline sequences due to inefficient peptide bond formation between consecutive prolines. The translation factor EF-P is able to alleviate this stalling by accelerating Pro-Pro formation. However, the mechanism by which EF-P recognizes the stalled complexes and accelerates pep...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms11657

    authors: Katoh T,Wohlgemuth I,Nagano M,Rodnina MV,Suga H

    更新日期:2016-05-24 00:00:00

  • Semiclassical Monte-Carlo approach for modelling non-adiabatic dynamics in extended molecules.

    abstract::Modelling of non-adiabatic dynamics in extended molecular systems and solids is a next frontier of atomistic electronic structure theory. The underlying numerical algorithms should operate only with a few quantities (that can be efficiently obtained from quantum chemistry), provide a controlled approximation (which ca...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms3144

    authors: Gorshkov VN,Tretiak S,Mozyrsky D

    更新日期:2013-01-01 00:00:00

  • Quasiparticle tunnel electroresistance in superconducting junctions.

    abstract::The term tunnel electroresistance (TER) denotes a fast, non-volatile, reversible resistance switching triggered by voltage pulses in ferroelectric tunnel junctions. It is explained by subtle mechanisms connected to the voltage-induced reversal of the ferroelectric polarization. Here we demonstrate that effects functio...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-14379-w

    authors: Rouco V,Hage RE,Sander A,Grandal J,Seurre K,Palermo X,Briatico J,Collin S,Trastoy J,Bouzehouane K,Buzdin AI,Singh G,Bergeal N,Feuillet-Palma C,Lesueur J,Leon C,Varela M,Santamaría J,Villegas JE

    更新日期:2020-01-31 00:00:00

  • Massive mining of publicly available RNA-seq data from human and mouse.

    abstract::RNA sequencing (RNA-seq) is the leading technology for genome-wide transcript quantification. However, publicly available RNA-seq data is currently provided mostly in raw form, a significant barrier for global and integrative retrospective analyses. ARCHS4 is a web resource that makes the majority of published RNA-seq...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-03751-6

    authors: Lachmann A,Torre D,Keenan AB,Jagodnik KM,Lee HJ,Wang L,Silverstein MC,Ma'ayan A

    更新日期:2018-04-10 00:00:00

  • Author Correction: Stochastic simulations reveal few green wave surfing populations among spring migrating herbivorous waterfowl.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Nature communications

    pub_type: 已发布勘误

    doi:10.1038/s41467-019-11151-7

    authors: Wang X,Cao L,Fox AD,Fuller R,Griffin L,Mitchell C,Zhao Y,Moon OK,Cabot D,Xu Z,Batbayar N,Kölzsch A,van der Jeugd HP,Madsen J,Chen L,Nathan R

    更新日期:2019-07-22 00:00:00

  • Unconventional valley-dependent optical selection rules and landau level mixing in bilayer graphene.

    abstract::Selection rules are of vital importance in determining the basic optical properties of atoms, molecules and semiconductors. They provide general insights into the symmetry of the system and the nature of relevant electronic states. A two-dimensional electron gas in a magnetic field is a model system where optical tran...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-16844-y

    authors: Ju L,Wang L,Li X,Moon S,Ozerov M,Lu Z,Taniguchi T,Watanabe K,Mueller E,Zhang F,Smirnov D,Rana F,McEuen PL

    更新日期:2020-06-10 00:00:00

  • Continuous crystalline graphene papers with gigapascal strength by intercalation modulated plasticization.

    abstract::Graphene has an extremely high in-plane strength yet considerable out-of-plane softness. High crystalline order of graphene assemblies is desired to utilize their in-plane properties, however, challenged by the easy formation of chaotic wrinkles for the intrinsic softness. Here, we find an intercalation modulated plas...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-020-16494-0

    authors: Li P,Yang M,Liu Y,Qin H,Liu J,Xu Z,Liu Y,Meng F,Lin J,Wang F,Gao C

    更新日期:2020-05-27 00:00:00

  • The DNA-binding network of Mycobacterium tuberculosis.

    abstract::Mycobacterium tuberculosis (MTB) infects 30% of all humans and kills someone every 20-30 s. Here we report genome-wide binding for ~80% of all predicted MTB transcription factors (TFs), and assayed global expression following induction of each TF. The MTB DNA-binding network consists of ~16,000 binding events from 154...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms6829

    authors: Minch KJ,Rustad TR,Peterson EJ,Winkler J,Reiss DJ,Ma S,Hickey M,Brabant W,Morrison B,Turkarslan S,Mawhinney C,Galagan JE,Price ND,Baliga NS,Sherman DR

    更新日期:2015-01-12 00:00:00

  • Quantifying through-space charge transfer dynamics in π-coupled molecular systems.

    abstract::Understanding the role of intermolecular interaction on through-space charge transfer characteristics in π-stacked molecular systems is central to the rational design of electronic materials. However, a quantitative study of charge transfer in such systems is often difficult because of poor control over molecular morp...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms2083

    authors: Batra A,Kladnik G,Vázquez H,Meisner JS,Floreano L,Nuckolls C,Cvetko D,Morgante A,Venkataraman L

    更新日期:2012-01-01 00:00:00

  • Quasi-specific access of the potassium channel inactivation gate.

    abstract::Many voltage-gated potassium channels open in response to membrane depolarization and then inactivate within milliseconds. Neurons use these channels to tune their excitability. In Shaker K(+) channels, inactivation is caused by the cytoplasmic amino terminus, termed the inactivation gate. Despite having four such gat...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms5050

    authors: Venkataraman G,Srikumar D,Holmgren M

    更新日期:2014-06-09 00:00:00

  • Archaeogenomic evidence reveals prehistoric matrilineal dynasty.

    abstract::For societies with writing systems, hereditary leadership is documented as one of the hallmarks of early political complexity and governance. In contrast, it is unknown whether hereditary succession played a role in the early formation of prehistoric complex societies that lacked writing. Here we use an archaeogenomic...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms14115

    authors: Kennett DJ,Plog S,George RJ,Culleton BJ,Watson AS,Skoglund P,Rohland N,Mallick S,Stewardson K,Kistler L,LeBlanc SA,Whiteley PM,Reich D,Perry GH

    更新日期:2017-02-21 00:00:00

  • Terrestrial land-cover type richness is positively linked to landscape-level functioning.

    abstract::Biodiversity-ecosystem functioning (BEF) experiments have shown that local species richness promotes ecosystem functioning and stability. Whether this also applies under real-world conditions is still debated. Here, we focus on larger scales of space, time and ecological organization. We develop a quasi-experimental d...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-14002-7

    authors: Oehri J,Schmid B,Schaepman-Strub G,Niklaus PA

    更新日期:2020-01-09 00:00:00

  • Ultrafast growth of nanocrystalline graphene films by quenching and grain-size-dependent strength and bandgap opening.

    abstract::Nanocrystallization is a well-known strategy to dramatically tune the properties of materials; however, the grain-size effect of graphene at the nanometer scale remains unknown experimentally because of the lack of nanocrystalline samples. Here we report an ultrafast growth of graphene films within a few seconds by qu...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-12662-z

    authors: Zhao T,Xu C,Ma W,Liu Z,Zhou T,Liu Z,Feng S,Zhu M,Kang N,Sun DM,Cheng HM,Ren W

    更新日期:2019-10-24 00:00:00

  • Multiple novel gene-by-environment interactions modify the effect of FTO variants on body mass index.

    abstract::Genetic studies have shown that obesity risk is heritable and that, of the many common variants now associated with body mass index, those in an intron of the fat mass and obesity-associated (FTO) gene have the largest effect. The size of the UK Biobank, and its joint measurement of genetic, anthropometric and lifesty...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms12724

    authors: Young AI,Wauthier F,Donnelly P

    更新日期:2016-09-06 00:00:00

  • Efficient and stable sky-blue delayed fluorescence organic light-emitting diodes with CIEy below 0.4.

    abstract::Organic light-emitting diodes utilizing thermally activated delayed fluorescence is a potential solution for achieving stable blue devices. Sky-blue devices (CIEy < 0.4) with high stability and high external quantum efficiency (>15%) at 1000 cd m-2 based on either delayed fluorescence or phosphorescence are still limi...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-018-07482-6

    authors: Chan CY,Tanaka M,Nakanotani H,Adachi C

    更新日期:2018-11-28 00:00:00

  • Publisher Correction: Aerodynamic generation of electric fields in turbulence laden with charged inertial particles.

    abstract::The original version of this Article contained an error in the last sentence of the second paragraph of the 'Atmospheric rarefaction effects' section of the Results, which incorrectly read 'The other one emulates the rarefied, CO2-rich Martian atmosphere (μ♂ = 1.3 × 10-5 N s m-2) at 6.9 mbar and 210 K, which gives ρ♂ ...

    journal_title:Nature communications

    pub_type: 杂志文章,已发布勘误

    doi:10.1038/s41467-018-04545-6

    authors: Di Renzo M,Urzay J

    更新日期:2018-05-21 00:00:00

  • Redesigning the QA binding site of Photosystem II allows reduction of exogenous quinones.

    abstract::Strategies to harness photosynthesis from living organisms to generate electrical power have long been considered, yet efficiency remains low. Here, we aimed to reroute photosynthetic electron flow in photosynthetic organisms without compromising their phototrophic properties. We show that 2,6-dimethyl-p-benzoquinone ...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms15274

    authors: Fu HY,Picot D,Choquet Y,Longatte G,Sayegh A,Delacotte J,Guille-Collignon M,Lemaître F,Rappaport F,Wollman FA

    更新日期:2017-05-03 00:00:00

  • Over-exploitation of natural resources is followed by inevitable declines in economic growth and discount rate.

    abstract::A major challenge in environmental policymaking is determining whether and how fast our society should adopt sustainable management methods. These decisions may have long-lasting effects on the environment, and therefore, they depend critically on the discount factor, which determines the relative values given to futu...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-09246-2

    authors: Lampert A

    更新日期:2019-03-29 00:00:00

  • Imaging with a small number of photons.

    abstract::Low-light-level imaging techniques have application in many diverse fields, ranging from biological sciences to security. A high-quality digital camera based on a multi-megapixel array will typically record an image by collecting of order 10(5) photons per pixel, but by how much could this photon flux be reduced? In t...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms6913

    authors: Morris PA,Aspden RS,Bell JE,Boyd RW,Padgett MJ

    更新日期:2015-01-05 00:00:00

  • Bacterial outer membrane proteins assemble via asymmetric interactions with the BamA β-barrel.

    abstract::The integration of β-barrel proteins into the bacterial outer membrane (OM) is catalysed by the β-barrel assembly machinery (BAM). The central BAM subunit (BamA) itself contains a β-barrel domain that is essential for OM protein biogenesis, but its mechanism of action is unknown. To elucidate its function, here we dev...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-11230-9

    authors: Doyle MT,Bernstein HD

    更新日期:2019-07-26 00:00:00

  • Immune-mediated genetic pathways resulting in pulmonary function impairment increase lung cancer susceptibility.

    abstract::Impaired lung function is often caused by cigarette smoking, making it challenging to disentangle its role in lung cancer susceptibility. Investigation of the shared genetic basis of these phenotypes in the UK Biobank and International Lung Cancer Consortium (29,266 cases, 56,450 controls) shows that lung cancer is ge...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/s41467-019-13855-2

    authors: Kachuri L,Johansson M,Rashkin SR,Graff RE,Bossé Y,Manem V,Caporaso NE,Landi MT,Christiani DC,Vineis P,Liu G,Scelo G,Zaridze D,Shete SS,Albanes D,Aldrich MC,Tardón A,Rennert G,Chen C,Goodman GE,Doherty JA,Bickebö

    更新日期:2020-01-07 00:00:00

  • Local mapping of detector response for reliable quantum state estimation.

    abstract::Improved measurement techniques are central to technological development and foundational scientific exploration. Quantum physics relies on detectors sensitive to non-classical features of systems, enabling precise tests of physical laws and quantum-enhanced technologies including precision measurement and secure comm...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms5332

    authors: Cooper M,Karpiński M,Smith BJ

    更新日期:2014-07-14 00:00:00

  • Climate constrains the evolutionary history and biodiversity of crocodylians.

    abstract::The fossil record of crocodylians and their relatives (pseudosuchians) reveals a rich evolutionary history, prompting questions about causes of long-term decline to their present-day low biodiversity. We analyse climatic drivers of subsampled pseudosuchian biodiversity over their 250 million year history, using a comp...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms9438

    authors: Mannion PD,Benson RBJ,Carrano MT,Tennant JP,Judd J,Butler RJ

    更新日期:2015-09-24 00:00:00

  • Publisher Correction: Reconciling qualitative, abstract, and scalable modeling of biological networks.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Nature communications

    pub_type: 已发布勘误

    doi:10.1038/s41467-020-18766-1

    authors: Paulevé L,Kolčák J,Chatain T,Haar S

    更新日期:2020-09-24 00:00:00

  • The key role of the scaffold on the efficiency of dendrimer nanodrugs.

    abstract::Dendrimers are well-defined macromolecules whose highly branched structure is reminiscent of many natural structures, such as trees, dendritic cells, neurons or the networks of kidneys and lungs. Nature has privileged such branched structures for increasing the efficiency of exchanges with the external medium; thus, t...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms8722

    authors: Caminade AM,Fruchon S,Turrin CO,Poupot M,Ouali A,Maraval A,Garzoni M,Maly M,Furer V,Kovalenko V,Majoral JP,Pavan GM,Poupot R

    更新日期:2015-07-14 00:00:00

  • Author Correction: P53 and mTOR signalling determine fitness selection through cell competition during early mouse embryonic development.

    abstract::The original version of this article contained an error in the spelling of Juan Pedro Martinez-Barbera, which was incorrectly given as Juan Pedro Martinez Barbera. This error has now been corrected in both the PDF and HTML versions of the Article. ...

    journal_title:Nature communications

    pub_type: 杂志文章,已发布勘误

    doi:10.1038/s41467-018-05718-z

    authors: Bowling S,Di Gregorio A,Sancho M,Pozzi S,Aarts M,Signore M,Schneider MD,Martinez-Barbera JP,Gil J,Rodríguez TA

    更新日期:2018-08-02 00:00:00

  • Energetics of activation of GTP hydrolysis on the ribosome.

    abstract::Several of the steps in protein synthesis on the ribosome utilize hydrolysis of guanosine triphosphate (GTP) as the driving force. This reaction is catalyzed by translation factors that become activated upon binding to the ribosome. The recently determined crystal structure of an elongation factor-Tu ternary complex b...

    journal_title:Nature communications

    pub_type: 杂志文章

    doi:10.1038/ncomms2741

    authors: Wallin G,Kamerlin SC,Aqvist J

    更新日期:2013-01-01 00:00:00