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An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.

Abstract：

:Protein glycosylation is ubiquitous in biological systems and essential for cell survival. However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis. Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species. Here, we develop a strategy using dendrimer-conjugated benzoboroxole to enhance the glycopeptide enrichment. We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates. The enrichment is further improved by conjugating benzoboroxole to a dendrimer, which enables synergistic benzoboroxole-glycan interactions. This robust and simple method is highly effective for sensitive glycoproteomics analysis, especially capturing low-abundance glycopeptides. Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures.

journal_name

Nat Commun

journal_title

Nature communications

authors

Xiao H,Chen W,Smeekens JM,Wu R

doi

10.1038/s41467-018-04081-3

subject

Has Abstract

pub_date

2018-04-27 00:00:00

pages

1692

issue

1

issn

2041-1723

pii

10.1038/s41467-018-04081-3

journal_volume

9

pub_type

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