Abstract：
:Because monoclonal antibodies (mAbs) have exceptional specificity and favorable pharmacology, substantial efforts have been made to functionalize them, either with potent cytotoxins, biologics, radionuclides, or fluorescent groups for therapeutic benefit and/or use as theranostic agents. To exploit our recently discovered meditope-Fab interaction as an alternative means to efficiently functionalize mAbs, we used insights from the structure to enhance the affinity and lifetime of the interaction by four orders of magnitude. To further extend the lifetime of the complex, we created a mechanical bond by incorporating an azide on the meditope, threading the azide through the Fab, and using click chemistry to add a steric group. The mechanically interlocked, meditope-Fab complex retains antigen specificity and is capable of imaging tumors in mice. These studies indicate it is possible to "snap" functionality onto mAbs, opening the possibility of rapidly creating unique combinations of mAbs with an array of cytotoxins, biologics, and imaging agents.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Bzymek KP,Puckett JW,Zer C,Xie J,Ma Y,King JD,Goodstein LH,Avery KN,Colcher D,Singh G,Horne DA,Williams JCdoi
10.1038/s41467-018-03976-5subject
Has Abstractpub_date
2018-04-20 00:00:00pages
1580issue
1issn
2041-1723pii
10.1038/s41467-018-03976-5journal_volume
9pub_type
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