Abstract:
:The spasmolytic activity of synthetic alpha-human atrial natriuretic peptide (alpha-hANP) and its related peptides was determined in vitro using the chick rectum and the rat aorta. Natriuretic activity was also measured in the anesthetized rat, alpha-hANP-(7-28), with the NH2-terminal hexapeptide truncated, had greater spasmolytic and natriuretic effect than did alpha-hANP-(1-28). These responses were reduced by truncation of the COOH-terminal residues. alpha-hANP-(7-23), the cyclic structure of alpha-hANP-(1-28), exhibited weak aortic relaxation and natriuretic activities. However, alpha-hANP-(7-23) produced a greater relaxation than did alpha-hANP-(1-28) in the chick rectum. Elimination of Gly at position 9 reduced the spasmolytic and natriuretic activity. Substitution of amino acid residues at position 8, 12 and 13 changed these activities. Analogues containing the ethylene linkage instead of the disulphide bond had weak biological activity. These results indicate that the size of the 17-amino acid ring and the COOH-terminal residues of alpha-hANP are important for the expression of spasmolytic and natriuretic activity.
journal_name
Eur J Pharmacoljournal_title
European journal of pharmacologyauthors
Watanabe TX,Noda Y,Chino N,Nishiuchi Y,Kimura T,Sakakibara S,Imai Mdoi
10.1016/0014-2999(88)90632-2subject
Has Abstractpub_date
1988-02-16 00:00:00pages
49-57issue
1eissn
0014-2999issn
1879-0712pii
0014-2999(88)90632-2journal_volume
147pub_type
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