Abstract：
:The progressive decline of the nervous system, including protein aggregate formation, reflects the subtle dysregulation of multiple functional pathways. Our previous work has shown intermittent fasting (IF) enhances longevity, maintains adult behaviors and reduces aggregates, in part, by promoting autophagic function in the aging Drosophila brain. To clarify the impact that IF-treatment has upon aging, we used high throughput RNA-sequencing technology to examine the changing transcriptome in adult Drosophila tissues. Principle component analysis (PCA) and other analyses showed ~1200 age-related transcriptional differences in head and muscle tissues, with few genes having matching expression patterns. Pathway components showing age-dependent expression differences were involved with stress response, metabolic, neural and chromatin remodeling functions. Middle-aged tissues also showed a significant increase in transcriptional drift-variance (TD), which in the CNS included multiple proteolytic pathway components. Overall, IF-treatment had a demonstrably positive impact on aged transcriptomes, partly ameliorating both fold and variance changes. Consistent with these findings, aged IF-treated flies displayed more youthful metabolic, behavioral and basal proteolytic profiles that closely correlated with transcriptional alterations to key components. These results indicate that even modest dietary changes can have therapeutic consequences, slowing the progressive decline of multiple cellular systems, including proteostasis in the aging nervous system.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Zhang S,Ratliff EP,Molina B,El-Mecharrafie N,Mastroianni J,Kotzebue RW,Achal M,Mauntz RE,Gonzalez A,Barekat A,Bray WA,Macias AM,Daugherty D,Harris GL,Edwards RA,Finley KDdoi
10.3390/ijms19041140subject
Has Abstractpub_date
2018-04-10 00:00:00issue
4issn
1422-0067pii
ijms19041140journal_volume
19pub_type
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