Helraiser intermediates provide insight into the mechanism of eukaryotic replicative transposition.

Abstract:

:Helitrons are eukaryotic DNA transposons that have profoundly affected genome variability via capture and mobilization of host genomic sequences. Defining their mode of action is therefore important for understanding how genome landscapes evolve. Sequence similarities with certain prokaryotic mobile elements suggest a "rolling circle" mode of transposition, involving only a single transposon strand. Using the reconstituted Helraiser transposon to study Helitron transposition in cells and in vitro, we show that the donor site must be double-stranded and that single-stranded donors will not suffice. Nevertheless, replication and integration assays demonstrate the use of only one of the transposon donor strands. Furthermore, repeated reuse of Helraiser donor sites occurs following DNA synthesis. In cells, circular double-stranded intermediates that serve as transposon donors are generated and replicated by Helraiser transposase. Cell-free experiments demonstrate strand-specific cleavage and strand transfer, supporting observations made in cells.

journal_name

Nat Commun

journal_title

Nature communications

authors

Grabundzija I,Hickman AB,Dyda F

doi

10.1038/s41467-018-03688-w

subject

Has Abstract

pub_date

2018-03-29 00:00:00

pages

1278

issue

1

issn

2041-1723

pii

10.1038/s41467-018-03688-w

journal_volume

9

pub_type

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