Abstract:
:Helitrons are eukaryotic DNA transposons that have profoundly affected genome variability via capture and mobilization of host genomic sequences. Defining their mode of action is therefore important for understanding how genome landscapes evolve. Sequence similarities with certain prokaryotic mobile elements suggest a "rolling circle" mode of transposition, involving only a single transposon strand. Using the reconstituted Helraiser transposon to study Helitron transposition in cells and in vitro, we show that the donor site must be double-stranded and that single-stranded donors will not suffice. Nevertheless, replication and integration assays demonstrate the use of only one of the transposon donor strands. Furthermore, repeated reuse of Helraiser donor sites occurs following DNA synthesis. In cells, circular double-stranded intermediates that serve as transposon donors are generated and replicated by Helraiser transposase. Cell-free experiments demonstrate strand-specific cleavage and strand transfer, supporting observations made in cells.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Grabundzija I,Hickman AB,Dyda Fdoi
10.1038/s41467-018-03688-wsubject
Has Abstractpub_date
2018-03-29 00:00:00pages
1278issue
1issn
2041-1723pii
10.1038/s41467-018-03688-wjournal_volume
9pub_type
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