Abstract:
:Bisphenol AF (BPAF) has been shown to inhibit testicular steroidogenesis in male rats. However, the precise mechanisms related to the toxic effects of BPAF on reproduction remain poorly understood. In the present study, a mouse Leydig tumor cell line (mLTC-1) was used as a model to investigate the mechanism of steroidogenic inhibition and to identify the molecular target of BPAF. Levels of progesterone and the concentration of cyclic adenosine monophosphate (cAMP) in cells exposed to BPAF were detected, and expression of key genes and proteins in steroid biosynthesis was assessed. The results showed that BPAF exposure decreased human chorionic gonadotrophin (hCG)-stimulated progesterone production in a dose-dependent manner. The 24-h IC50 (half maximal inhibitory concentration) value for BPAF regarding progesterone production was 70.2 µM. A dramatic decrease in cellular cAMP concentration was also observed. Furthermore, BPAF exposure inhibited expression of genes and proteins involved in cholesterol transport and progesterone biosynthesis. Conversely, the protein levels of steroidogenic acute regulatory protein (StAR) were not altered, and those of progesterone were still decreased upon 22R-hydroxycholesterol treatment of cells exposed to higher doses of BPAF. Together, these data indicate that BPAF exposure inhibits progesterone secretion in hCG-stimulated mLTC-1 cells by reducing expression of scavenger receptor class B type I (SR-B1) and cytochrome P450 (P450scc) due to the adverse effects of cAMP. However, StAR might not be the molecular target in this process.
journal_name
Environ Toxicoljournal_title
Environmental toxicologyauthors
Feng Y,Shi J,Jiao Z,Duan H,Shao Bdoi
10.1002/tox.22554subject
Has Abstractpub_date
2018-06-01 00:00:00pages
670-678issue
6eissn
1520-4081issn
1522-7278journal_volume
33pub_type
杂志文章abstract::Effects of perchlorate on sodium-iodide symporter (NIS) and pendrin gene expression in deer mice kidney and stomach were investigated. This was accomplished by isolating a partial cDNA sequence of deer mice NIS gene of 425 bps, and quantitatively analyzing NIS mRNA expression in various deer mouse tissues. The highest...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20271
更新日期:2007-08-01 00:00:00
abstract::Acetaminophen (APAP) is one of the few recommended analgesic and antipyretic drugs in some critical cases such as viral disease COVID-19. However, the unrestricted use of APAP develops liver disorders. Hepatotoxicity and liver injury can also be induced by ionizing radiation (IR) during radiotherapy. The data of the c...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.23077
更新日期:2020-12-17 00:00:00
abstract::Metal oxide nanoparticles and carbon nanoparticles, as common nanoparticles (NPs), can cause autophagy in certain cells, which will lead to biohealth risk issues. This study determined the difference in autophagy induced by zinc oxide nanoparticles (ZnO NPs) and single-walled carbon nanotubes (SWCNTs) in respiratory e...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.23029
更新日期:2021-02-01 00:00:00
abstract::Within the last decade, numerous epidemiological studies have demonstrated that endocrine disruptors are a possible cause for a decline in semen quality. Cypermethrin is a widely used pyrethroid insecticide, but little is known about its potentially adverse effects on male reproduction. In the present study, we invest...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20566
更新日期:2011-08-01 00:00:00
abstract::The sensitivities of juvenile and adult amphipods to metals (Cd, Cu, Ni, Pb, and Zn) in whole sediment and water-only exposures were compared using a newly developed acute test with the estuarine species Melita plumulosa. Endpoints included survival and bioaccumulation in adults, and survival and growth (body length) ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20211
更新日期:2006-10-01 00:00:00
abstract::Nonsmall cell lung cancer (NSCLC) is the most common type of lung cancer with a high mortality rate and still remains a therapeutic challenge. A strategy for targeting NSCLC is to identify agents that are effective against NSCLC cells while sparing normal cells. Dihydromyricetin (DHM) is the major flavonoid component ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22336
更新日期:2017-04-01 00:00:00
abstract::The microbial assay for risk assessment (MARA) is an innovative system based on an array of 11 different microbial species freeze-dried in a 96-well micro-titer plate. Developed for testing the toxicity of chemicals, mixtures, and environmental samples, the assay employs species of a taxonomically diverse range. In ad...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20419
更新日期:2008-10-01 00:00:00
abstract::Structure-activity relationships for oestrogenicity were developed based on 120 aromatic chemicals evaluated in the Saccharomyces cerevisiae-based Lac-Z reporter assay. Relative gene activation was compared to 17 beta-estradiol and varied over eight orders of magnitude. Analysis of the data compared to 17 beta-estradi...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.10027
更新日期:2002-02-01 00:00:00
abstract::The 8-hydroxy-2'-deoxyguanosine (8-OHdG), an oxidized nucleoside of DNA, not only is a widely used biomarker for the measurement of endogenous oxidative DNA damage, but might also be a risk factor for many diseases including cancer. Elevated level of urinary 8-OHdG has been detected in patients with various malignanci...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20447
更新日期:2009-10-01 00:00:00
abstract::As a widely used plasticizer in industry, di-2-ethylhexylphthalate (DEHP) can cause testicular toxicity, yet little is known about the potential mechanism. In this study, DEHP exposure dramatically inhibited cellviability and induced apoptosis of mouse GC-1 spg cells. Furthermore, DEHP significantly increased the leve...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22866
更新日期:2020-02-01 00:00:00
abstract::This study investigated lethal effects (i.e., survival) and sublethal effects (glutathione, GSH; lipid peroxidation, LPx; cholesterol, CHL; and acetylcholinesterase, AChE) of the antihyperlipidemic drug simvastatin on larval and adult grass shrimp (Palaemonetes pugio). The 96-h LC50 test for larvae resulted in an esti...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20318
更新日期:2008-04-01 00:00:00
abstract::The cytotoxic and genotoxic effects induced by alachlor and dichlorvos, two pesticides broadly used, were studied on RTG-2 fish cell line. As measure of cytotoxicity, neutral red assay was used to determine the cellular viability. Toxicity ranking based on IC(50) values found that alachlor was more cytotoxic than dich...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20637
更新日期:2012-03-01 00:00:00
abstract::Oral cancer is one of the cancer-related diseases in human populations and its incidence rates are rising worldwide. Fisetin, a flavonoid from natural products, has been shown to exhibit anticancer activities in many human cancer cell lines but the molecular mechanism of fisetin-induced apoptosis in human oral cancer ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22396
更新日期:2017-06-01 00:00:00
abstract::Bisphenol A (BPA) and cadmium (Cd) are environmental pollutants that are implicated in potential reproductive effects, including damage to the prostate gland. Their action during puberty requires analysis to determine the relationship of these compounds with the testosterone peak that occurs during this phase. This st...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22211
更新日期:2017-01-01 00:00:00
abstract::Long non-coding RNA (lncRNA) plays a critical role in male germline development. Atrazine (AZ) as an environmental endocrine disrupting chemical (EDCs) can induce male reproductive toxicity in amphibians. Our previous studies demonstrated that AZ can alter gene and circular RNA (circRNA) expression of damaged testes i...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22704
更新日期:2019-04-01 00:00:00
abstract::The aim of this study was to determine whether repeated exposure to iron oxide nanoparticles (Fe2 O3 -NPs) could be toxic to mice testis. Fe2 O3 -NPs (25 and 50 mg/kg) were intraperitoneally administered into mice once a week for 4 weeks. Our study showed that Fe2 O3 -NPs have the ability to cross the blood-testis bar...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22262
更新日期:2017-02-01 00:00:00
abstract::Particulate matter the environmental toxicant, with a diameter less than or equal to 2.5 μm (PM2.5 ) is a common cause of several respiratory diseases. In recent years, several studies have suggested that PM2.5 can influence diverse diseases, such as respiratory diseases, cardiovascular diseases, metabolic diseases, d...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22591
更新日期:2018-11-01 00:00:00
abstract::The acute toxicity of cylindrospermopsin (CYN) has been established in rodents, based on diverse intraperitoneal an oral exposure studies and more recently in fish. But no data have been reported in fish after subchronic exposure to cyanobacterial cells containing this cyanotoxin, so far. In this work, tilapia (Oreoch...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21904
更新日期:2015-03-01 00:00:00
abstract::di-N-butylphthalate (DBP) is a ubiquitous environmental pollutant used for plastic coating and in the cosmetics industry. It has toxic effects on body health, especially the male reproductive system. Here, we investigated the effects of DBP on the male reproductive system of pubertal mice and explored the protective r...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22413
更新日期:2017-07-01 00:00:00
abstract::Recent epidemiological studies have revealed significant positive associations between exposure to organochlorine (OC) pesticides and occurrence of the metabolic syndrome and there are a growing number of animal-based studies to support causality. However, the cellular mechanisms linking OC compound exposure and metab...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22583
更新日期:2018-09-01 00:00:00
abstract::The genotoxic effects of a particular mixture of acetamiprid (Acm, neonicotinoid insecticide) and alpha-cypermethrin (alpha-cyp, pyrethroid insecticide) on human peripheral lymphocytes were examined in vitro by chromosomal aberrations (CAs), sister chromatid exchange (SCE), and micronucleus (MN) tests. The human perip...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20485
更新日期:2010-04-01 00:00:00
abstract::Dibutyltin (DBT) is the degradation products of TBT, which is generally considered higher toxicity than TBT in the immune system. In order to learn more about the mechanisms of immune-toxic of DBT, we exposed zebrafish (Danio rerio) to 0, 1, 10 and 100 ng/L DBT for 8 weeks. At the end of the experiment, we determined ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22502
更新日期:2018-01-01 00:00:00
abstract::Sulfur dioxide (SO2 ) is a ubiquitous air pollutant. The previous studies have documented the adverse effects of SO2 on nervous system health, suggesting that acutely SO2 inhalation at high concentration may be associated with neurotoxicity and increase risk of hospitalization and mortality of many brain disorders. Ho...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22093
更新日期:2016-07-01 00:00:00
abstract::Glyphosate is the active ingredient of several herbicide formulations. Different reports suggest that glyphosate-based herbicides (GBHs) may act as endocrine disruptors. We evaluated the potential estrogenic effects of a GBH formulation using the uterotrophic assay. Adult ovariectomized rats were sc injected for 3 con...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22316
更新日期:2017-04-01 00:00:00
abstract::Bisphenol A (BPA) has been widely reported to exert endocrine disrupting effects, including the induction of adipogenesis in cultured preadipocytes and intact animals. Because of the potential harm to human health, BPA is being substituted by structurally related bisphenols. Whether or not such BPA analogues are safe ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22889
更新日期:2020-05-01 00:00:00
abstract::Micronucleus induction by the diarrhetic shellfish toxin okadaic acid (OA) was investigated in two intestinal models, cultured human Caco-2 cells and colon epithelial cells of mice treated in vivo. Exposure to OA for 4 and 24 h induced dose-responsive increases in the frequency of micronucleated Caco-2 cells; the mini...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20154
更新日期:2006-02-01 00:00:00
abstract::For the first time, juvenile toxicity of inorganic arsenic (As) was investigated in male rats, focusing on reproductive effects. As is a metalloid naturally occurring in the environment, being the inorganic forms the most toxics. Contaminated drinking water and agricultural products are the main prospectors of intoxic...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22660
更新日期:2019-01-01 00:00:00
abstract::Benchmark dose (BMD) and the lower confidence limit on the benchmark dose (BMDL) of blood lead were estimated to explore the biologic exposure limits for renal dysfunction caused by lead. One hundred thirty-five workers from one storage battery plant were selected as lead exposure group while 143 mechanics as the cont...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20260
更新日期:2007-06-01 00:00:00
abstract::There is emerging evidence suggesting that oxidative stress and DNA methylation can alter miRNA expression. However, little is known on the mechanism of miR-17-5p expression changes in paraquat (PQ)-induced nerve cell damage. In the present study, neuro-2a cells were pretreated with antioxidant N-acetylcysteine (NAC) ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.23001
更新日期:2020-12-01 00:00:00
abstract::Thallium (Tl) is a highly toxic heavy metal though up to now its mechanisms are poorly understood. In this study, we comparatively investigated the cytotoxic mechanisms of Tl(I) and Tl(III) in isolated rat hepatocytes. Both Tl(I) and Tl(III) cytotoxicities were associated with reactive oxygen species (ROS) formation, ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20590
更新日期:2010-10-01 00:00:00