Analysis of oscillatory rocking curve by dynamical diffraction in protein crystals.

abstract：:Gene expression in higher organisms is thought to be regulated by a complex network of transcription factor binding and chromatin modifications, yet the relative importance of these two factors remains a matter of debate. Here, we show that a computational approach allows surprisingly accurate prediction of histone mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benveniste D,Sonntag HJ,Sanguinetti G,Sproul D

更新日期：2014-09-16 00:00:00

abstract：:Microbial pathogens with the ability to establish chronic infections have evolved strategies to actively modulate the host immune response. Brucellosis is a disease caused by a Gram-negative intracellular pathogen that if not treated during the initial phase of the infection becomes chronic as the bacteria persist for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spera JM,Ugalde JE,Mucci J,Comerci DJ,Ugalde RA

更新日期：2006-10-31 00:00:00

abstract：:We have developed a cell-free system to study bacteriophage lambda DNA replication. Maximal DNA synthesis in vitro requires the four deoxynucleoside triphosphates, ATP, and exogenous lambda DNA. DNA synthesis requires the products of the phage O and P genes but is not inhibited by lambda repressor. The kinetics of syn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shizuya H,Richardson CC

更新日期：1974-05-01 00:00:00

abstract：:The Sleeping Beauty (SB) transposon system can integrate foreign sequences of DNA in the genome of mouse somatic cells eliciting long-term expression in vivo. This technology holds great promise for human gene therapy as a nonviral technology to deliver therapeutic genes. SB also provides a means to study the effects ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carlson CM,Frandsen JL,Kirchhof N,McIvor RS,Largaespada DA

更新日期：2005-11-22 00:00:00

abstract：:RNA is involved in a variety of chromatin modification events, ranging from large-scale structural rearrangements to subtle local affects. Here, we extend the evidence for RNA-chromatin interactions to the centromere core. The data indicate that maize centromeric retrotransposons (CRMs) and satellite repeats (CentC) a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Topp CN,Zhong CX,Dawe RK

更新日期：2004-11-09 00:00:00

abstract：:Despite the importance of tuberculosis as a public health problem, we know relatively little about the molecular mechanisms used by the causative organism, Mycobacterium tuberculosis, to persist in the host. To define these mechanisms, we have mutated virtually every nonessential gene of M. tuberculosis and determined...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sassetti CM,Rubin EJ

更新日期：2003-10-28 00:00:00

abstract：:Degeneracy, the ability of elements that are structurally different to perform the same function or yield the same output, is a well known characteristic of the genetic code and immune systems. Here, we point out that degeneracy is a ubiquitous biological property and argue that it is a feature of complexity at geneti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edelman GM,Gally JA

更新日期：2001-11-20 00:00:00

abstract：:During sporulation of the Gram-positive bacterium Bacillus subtilis, transcription of genes encoding spore coat proteins in the mother-cell compartment of the sporangium is controlled by RNA polymerase containing the sigma subunit called sigma K. Based on comparison of the N-terminal amino acid sequence of sigma K wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu S,Halberg R,Kroos L

更新日期：1990-12-01 00:00:00

abstract：:A novel OTX-related homeodomain transcription factor has been identified on the basis of its ability to interact with the transactivation domain of the pituitary-specific POU domain protein, Pit-1. This factor, referred to as P-OTX (pituitary OTX-related factor), is expressed in primordial Rathke's pouch, oral epithel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szeto DP,Ryan AK,O'Connell SM,Rosenfeld MG

更新日期：1996-07-23 00:00:00

abstract：:The effect of fluctuations in rate constants on the kinetic behavior of cyclic reacting systems, caused by a fluctuating external parameter, is studied. It is shown (i) that the stochastic properties of the system can be analyzed analytically by using the usual master equation approach when the external parameter is f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen YD

更新日期：1987-02-01 00:00:00

abstract：:The involvement of p53 in regulating diverse cellular processes dictates that it must respond to multiple signaling mechanisms, thus coordinating the response to various "stress conditions." Genotoxic stress has served as a paradigm to dissect the transactivation-dependent branch of the pathway by which p53 can induce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruaro EM,Collavin L,Del Sal G,Haffner R,Oren M,Levine AJ,Schneider C

更新日期：1997-04-29 00:00:00

abstract：:Mutations in the low density lipoprotein (LDL) receptor (LDLR) cause hypercholesterolemia because of inefficient LDL clearance from the circulation. In addition, there is a paradoxical oversecretion of the metabolic precursor of LDL, very low density lipoprotein (VLDL). We recently demonstrated that the LDLR mediates ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gillian-Daniel DL,Bates PW,Tebon A,Attie AD

更新日期：2002-04-02 00:00:00

abstract：:Twenty-five years ago this month, Thomas Gold published a seminal manuscript suggesting the presence of a "deep, hot biosphere" in the Earth's crust. Since this publication, a considerable amount of attention has been given to the study of deep biospheres, their role in geochemical cycles, and their potential to infor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colman DR,Poudel S,Stamps BW,Boyd ES,Spear JR

更新日期：2017-07-03 00:00:00

abstract：:Chemoresistance of cisplatin therapy is related to extensive repair of cisplatin-modified DNA in the nucleus by the nucleotide excision repair (NER). Delivering cisplatin to the mitochondria to attack mitochondrial genome lacking NER machinery can lead to a rationally designed therapy for metastatic, chemoresistant ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marrache S,Pathak RK,Dhar S

更新日期：2014-07-22 00:00:00

abstract：:A method has been developed that enables us to identify intracellular degradation intermediates of fructose-bisphosphate aldolase B (D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate-lyase, EC 4.1.2.13). This method is based on the use of antibody against thoroughly denatured purified aldolase. This antibody ha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reznick AZ,Rosenfelder L,Shpund S,Gershon D

更新日期：1985-09-01 00:00:00

abstract：:We describe a method for predicting the structure of alpha beta class proteins in the absence of information from homologous structures. The method is based on an associative memory model for short to intermediate range in sequence contacts and a contact potential for long range in sequence contacts. The coefficients ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hardin C,Eastwood MP,Prentiss MC,Luthey-Schulten Z,Wolynes PG

更新日期：2003-02-18 00:00:00

abstract：:Elucidating the lineage relationships among different cell types is key to understanding human brain development. Here we developed parallel RNA and DNA analysis after deep sequencing (PRDD-seq), which combines RNA analysis of neuronal cell types with analysis of nested spontaneous DNA somatic mutations as cell lineag...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang AY,Li P,Rodin RE,Kim SN,Dou Y,Kenny CJ,Akula SK,Hodge RD,Bakken TE,Miller JA,Lein ES,Park PJ,Lee EA,Walsh CA

更新日期：2020-06-23 00:00:00

abstract：:Many experiments have been done to determine how far and how freely holes can move along the stack of base pairs in DNA. The results of these experiments are usually described in terms of a parameter beta under the assumption that it describes an exponential decay with distance. The reported values range from beta < 0...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conwell EM,Rakhmanova SV

更新日期：2000-04-25 00:00:00

abstract：:To evaluate [3H]phencyclidine ([3H]PCP)as a probe for the ionic channel of the nicotinic receptor, the characteristics of its binding to electric organ membranes od Torpedo ocellata and its effects on frog sartorius muscle were studied. Similar to PCP, [3H]PCP depressed the peak amplitude of endplate current, caused n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eldefrawi ME,Eldefrawi AT,Aronstam RS,Maleque MA,Warnick JE,Albuquerque EX

更新日期：1980-12-01 00:00:00

abstract：:The human brain relies upon the dynamic formation and dissolution of a hierarchy of functional networks to support ongoing cognition. However, how functional connectivities underlying such networks are supported by cortical microstructure remains poorly understood. Recent animal work has demonstrated that electrical a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hunt BA,Tewarie PK,Mougin OE,Geades N,Jones DK,Singh KD,Morris PG,Gowland PA,Brookes MJ

更新日期：2016-11-22 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDAC) remains a highly lethal disease in critical need of new therapeutic strategies. Here, we report that the stress-inducible 78-kDa glucose-regulated protein (GRP78/HSPA5), a key regulator of endoplasmic reticulum homeostasis and PI3K/AKT signaling, is overexpressed in the acini an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen J,Ha DP,Zhu G,Rangel DF,Kobielak A,Gill PS,Groshen S,Dubeau L,Lee AS

更新日期：2017-05-16 00:00:00

abstract：:Most higher eukaryotic tubulins are separated into alpha- and beta-tubulin when electrophoresed in NaDodSO4- denaturing gels, while many lower eukaryotic tubulins are poorly resolved under these conditions, which include a stacking gel (pH 6.80) and a separating gel (pH 8.80). By lowering the pH of the separating gel ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suprenant KA,Hays E,LeCluyse E,Dentler WL

更新日期：1985-10-01 00:00:00

abstract：:Brown adipose tissue (BAT) is present throughout life in rodents and plays an important role in energy balance. However, whereas BAT is clearly recognizable in the neonates of larger mammals (including dogs, cats, sheep, cattle, and humans), it is undetectable or present in only small quantities in adults of these spe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Champigny O,Ricquier D,Blondel O,Mayers RM,Briscoe MG,Holloway BR

更新日期：1991-12-01 00:00:00

abstract：:2-Isopropylmalate synthase (EC 4.1.3.12) of yeast is a mitochondrial enzyme. We now provide evidence showing that a large part of the 2-isopropylmalate synthase activity that is associated with the mitochondria is located in the mitochondrial matrix. In vitro translation of total yeast RNA followed by immunoprecipitat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hampsey DM,Lewin AS,Kohlhaw GB

更新日期：1983-03-01 00:00:00

abstract：:Recent observations suggest that p53 mutations are responsible not only for growth of primary tumors but also for their dissemination. However, mechanisms involved in p53-mediated control of cell motility and invasion remain poorly understood. By using the primary ovarian surface epithelium cell culture, we show that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang CI,Matoso A,Corney DC,Flesken-Nikitin A,Körner S,Wang W,Boccaccio C,Thorgeirsson SS,Comoglio PM,Hermeking H,Nikitin AY

更新日期：2011-08-23 00:00:00

abstract：:Xanthophyll pigments have critical structural and functional roles in the photosynthetic light-harvesting complexes of algae and vascular plants. Genetic dissection of xanthophyll metabolism in the green alga Chlamydomonas reinhardtii revealed functions for specific xanthophylls in the nonradiative dissipation of exce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Niyogi KK,Björkman O,Grossman AR

更新日期：1997-12-09 00:00:00

abstract：:A low molecular weight toxin isolated from the venom of the digger wasp Philanthus triangulum, first noted by T. Piek, is a potent antagonist of transmission at quisqualate-sensitive glutamate synapses of locust leg muscle. This philanthotoxin 433 (PTX-433) has been purified, chemically characterized, and subsequently...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eldefrawi AT,Eldefrawi ME,Konno K,Mansour NA,Nakanishi K,Oltz E,Usherwood PN

更新日期：1988-07-01 00:00:00

abstract：:Arsenic has played a key medicinal role against a variety of ailments for several millennia, but during the past century its prominence has been displaced by modern therapeutics. Recently, attention has been drawn to arsenic by its dramatic clinical efficacy against acute promyelocytic leukemia. Although toxic reactiv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chou WC,Jie C,Kenedy AA,Jones RJ,Trush MA,Dang CV

更新日期：2004-03-30 00:00:00

abstract：:Many materials phenomena are governed by the interaction between chemistry and mechanics. However, it was only in the second half of the 20th century that the theory of open system elasticity by Francis Larché and John W. Cahn concatenated the fields of solid mechanics and alloy chemistry. As the theory's central mate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi S,Markmann J,Weissmüller J

更新日期：2018-10-23 00:00:00

abstract：:The ASCAPE agent-based modeling environment greatly eases the task of designing and investigating agent-based models. However, effective design can still require a relatively deep knowledge of programming and agent-based modeling. This issue has almost certainly slowed the adoption of agent-based modeling approaches t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inchiosa ME,Parker MT

更新日期：2002-05-14 00:00:00