Abstract：
:Mammalian respiratory complex I (CI) biogenesis requires both nuclear and mitochondria-encoded proteins and is mostly organized in respiratory supercomplexes. Among the CI proteins encoded by the mitochondrial DNA, NADH-ubiquinone oxidoreductase chain 1 (ND1) is a core subunit, evolutionary conserved from bacteria to mammals. Recently, ND1 has been recognized as a pivotal subunit in maintaining the structural and functional interaction among the hydrophilic and hydrophobic CI arms. A critical role of human ND1 both in CI biogenesis and in the dynamic organization of supercomplexes has been depicted, although the proof of concept is still missing and the critical amount of ND1 protein necessary for a proper assembly of both CI and supercomplexes is not defined. By exploiting a unique model in which human ND1 is allotopically re-expressed in cells lacking the endogenous protein, we demonstrated that the lack of this protein induces a stall in the multi-step process of CI biogenesis, as well as the alteration of supramolecular organization of respiratory complexes. We also defined a mutation threshold for the m.3571insC truncative mutation in mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 1 (MT-ND1), below which CI and its supramolecular organization is recovered, strengthening the notion that a certain amount of human ND1 is required for CI and supercomplexes biogenesis.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Iommarini L,Ghelli A,Tropeano CV,Kurelac I,Leone G,Vidoni S,Lombes A,Zeviani M,Gasparre G,Porcelli AMdoi
10.3390/ijms19030764subject
Has Abstractpub_date
2018-03-07 00:00:00issue
3issn
1422-0067pii
ijms19030764journal_volume
19pub_type
杂志文章abstract：:This study aims to reveal the potential relationship between 5-HT and oxidative stress in the organism. Our in vitro experiments in RIN-14B cells showed that anoxia leads the cells to the state of oxidative stress. Administration of exogenous 5-HT exacerbated this effect, whereas the inhibition of Tph1, LP533401 allev...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20061364
更新日期：2019-03-18 00:00:00
abstract：:Fine needle aspiration cytology (FNAC) represents the gold standard for determining the nature of thyroid nodules. It is a reliable method with good sensitivity and specificity. However, indeterminate lesions remain a diagnostic challenge and researchers have contributed molecular markers to search for in cytological ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18040775
更新日期：2017-04-06 00:00:00
abstract：:Aeromonas veronii is a pathogen capable of infecting humans, livestock and aquatic animals, resulting in serious economic losses. In this study, two recombinant Lactobacillus casei expressing flagellin A (FlaA) of A. veronii, Lc-pPG-1-FlaA (surface-displayed) and Lc-pPG-2-FlaA (secretory) were constructed. The immune ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010033
更新日期：2019-12-19 00:00:00
abstract：:Blood vessels in vertebrates are established and genetically controlled in an evolutionarily-conserved manner during embryogenesis. Disruption of vascular growth by chemical compounds or environmental hormones may cause developmental defects. This study analyzed the vascular impacts of marine compound GB9 in zebrafish...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18081696
更新日期：2017-08-03 00:00:00
abstract：:Down syndrome (DS), the most common cause of intellectual disability of genetic origin, is characterized by alterations in central nervous system morphology and function that appear from early prenatal stages. However, by the fourth decade of life, all individuals with DS develop neuropathology identical to that found...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21186906
更新日期：2020-09-20 00:00:00
abstract：:In higher organisms, epithelia separate compartments in order to guarantee their proper function. Such structures are able to seal but also to allow substances to pass. Within the paracellular pathway, a supramolecular structure, the tight junction transport is largely controlled by the temporospatial regulation of it...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215504
更新日期：2019-11-05 00:00:00
abstract：:Bifidobacterium bifidum BGN4 is a probiotic strain that has been used as a major ingredient to produce nutraceutical products and as a dairy starter since 2000. The various bio-functional effects and potential for industrial application of B. bifidum BGN4 has been characterized and proven by in vitro (i.e., phytochemi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17091544
更新日期：2016-09-14 00:00:00
abstract：:Oxidative damages induced by a redox imbalance cause age-related changes in cells and tissues. Superoxide dismutase (SOD) enzymes play a major role in the antioxidant system and they also catalyze superoxide radicals (O2·-). Since the loss of cytoplasmic SOD (SOD1) resulted in aging-like phenotypes in several types of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140610998
更新日期：2013-05-24 00:00:00
abstract：:Land plants are anchored in one place for most of their life cycle and therefore must constantly adapt their growth and metabolism to abiotic stresses such as light intensity, temperature and the availability of water and essential minerals. Thus, plants' subsistence depends on their ability to regulate rapidly gene e...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms10073168
更新日期：2009-07-10 00:00:00
abstract：:The turnover of the RNA molecules is determined by the rates of transcription and RNA degradation. Several methods have been developed to study RNA turnover since the beginnings of molecular biology. Here we summarize the main methods to measure RNA half-life: transcription inhibition, gene control, and metabolic labe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18122723
更新日期：2017-12-15 00:00:00
abstract：:The N-methyl-(2S,4R)-trans-4-hydroxy-l-proline-enriched fraction (NMP) from Sideroxylon obtusifolium was evaluated as a neuroprotective agent in the intracerebroventricular (icv) pilocarpine (Pilo) model. To this aim, male mice were subdivided into sham (SO, vehicle), Pilo (300 µg/1 µL icv, followed by the vehicle per...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21114188
更新日期：2020-06-11 00:00:00
abstract：:The Prostate Health Index (PHI) has been used increasingly in the context of prostate cancer (PCa) diagnostics since 2010. Previous studies have shown an association between PHI and a tumor volume of >0.5 cm³. The aim of this study was to investigate the correlation between PHI and tumor volume as well as the Gleason ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18030488
更新日期：2017-02-24 00:00:00
abstract：:Bacterial adhesion to the calcium phosphate surface is a serious problem in surgery. To prevent bacterial infection, the development of calcium-phosphate cements (CPCs) with bactericidal properties is indispensable. The aim of this study was to fabricate antibacterial CPCs and evaluate their biological properties. Sil...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21113745
更新日期：2020-05-26 00:00:00
abstract：:Persistent inflammation is a complication associated with many ocular diseases. Changes in ocular vessels can amplify disease responses and contribute to vision loss by influencing the delivery of leukocytes to the eye, vascular leakage, and perfusion. Here, we report the anti-inflammatory activity for AXT107, a non-R...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21145142
更新日期：2020-07-21 00:00:00
abstract：:Binding affinity prediction of potential drugs to target and off-target proteins is an essential asset in drug development. These predictions require the calculation of binding free energies. In such calculations, it is a major challenge to properly account for both the dynamic nature of the protein and the possible v...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15010798
更新日期：2014-01-09 00:00:00
abstract：:The mushroom Inonotus obliquus has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. In this study, water-soluble and alkali-soluble crude polysaccharides (IOW and IOA) were isolated from I. obliquus, and the carbohydrate-rich fractions IOW-1 and IOA-1 were obtained respectively a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13079194
更新日期：2012-01-01 00:00:00
abstract：:Although the genetic alteration of CUB and Sushi multiple domains 1 (CSMD1) is known to be associated with poor prognosis in several cancers, there is a lack of clinical relevance in head and neck cancer. The aim of this study was to offer insight into the clinical significance of CSMD1, utilizing a multimodal approac...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19123996
更新日期：2018-12-12 00:00:00
abstract：:The rising demand for powerful oncolytic virotherapy agents has led to the identification of Maraba virus, one of the most potent oncolytic viruses from Rhabdoviridae family which displays high selectivity for killing malignant cells and low cytotoxicity in normal cells. Although the virus is readied to be used for cl...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030580
更新日期：2019-01-29 00:00:00
abstract：:While platelet function has traditionally been described in the context of maintaining vascular integrity, recent evidence suggests that platelets can modulate inflammation in a much more sophisticated and nuanced manner than previously thought. Some aspects of this expanded repertoire of platelet function are mediate...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21176150
更新日期：2020-08-26 00:00:00
abstract：:The recent emergence of antibiotic-resistant bacteria requires the development of new antibiotics or new agents capable of enhancing antibiotic activity. Lysozyme degrades bacterial cell wall without involving antibiotic resistance and has become a new antibacterial strategy. However, direct use of native, active prot...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051635
更新日期：2020-02-27 00:00:00
abstract：:The authors wish to make the following corrections to this paper [...]. ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,已发布勘误
doi：10.3390/ijms21124560
更新日期：2020-06-26 00:00:00
abstract：:Aging is a physiologic/pathologic process characterized by a progressive impairment of cellular functions, supported by the alterations of several molecular pathways, leading to an increased cell susceptibility to injury. This deterioration is the primary risk factor for several major human pathologies. Numerous cellu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20112774
更新日期：2019-06-06 00:00:00
abstract：:Future long-duration space missions will involve travel outside of the Earth's magnetosphere protection and will result in astronauts being exposed to high energy and charge (HZE) ions and protons. Exposure to this type of radiation can result in damage to the central nervous system and deficits in numerous cognitive ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20020339
更新日期：2019-01-15 00:00:00
abstract：:Dementia with Lewy bodies (DLB) is the second most prevalent neurodegenerative dementia after Alzheimer's disease, and is pathologically characterized by formation of intracellular inclusions called Lewy bodies, the major constituent of which is aggregated α-synuclein (αS). Currently, neither a mechanistic etiology no...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21082849
更新日期：2020-04-19 00:00:00
abstract：:Marine sponges remain representative of a unique source of renewable biological materials. The demosponges of the family Ianthellidae possess chitin-based skeletons with high biomimetic potential. These three-dimensional (3D) constructs can potentially be used in tissue engineering and regenerative medicine. In this s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205105
更新日期：2019-10-15 00:00:00
abstract：:With age, our cognitive skills and abilities decline. Maybe starting as an annoyance, this decline can become a major impediment to normal daily life. Recent research shows that the neurodegenerative disorders responsible for age associated cognitive dysfunction are mechanistically linked to the state of the microvasc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20215472
更新日期：2019-11-03 00:00:00
abstract：:A common problem in the immunodetection of structurally close compounds is understanding the regularities of immune recognition, and elucidating the basic structural elements that provide it. Correct identification of these elements would allow for select immunogens to obtain antibodies with either wide specificity to...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20020265
更新日期：2019-01-11 00:00:00
abstract：:3,3'-Diindolylmethane (DIM) is a naturally derived chemopreventive compound. It comes from glucobrassicin, an indole glucosinolate enriched in cruciferous vegetables, and is formed in the acidic environment of the stomach after ingestion. Mouse double minute 2 homolog (MDM2) is an important, multi-functional oncogenic...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21134642
更新日期：2020-06-30 00:00:00
abstract：:The adverse prognosis of most patients with ovarian cancer is related to recurrent disease caused by resistance to chemotherapeutic and targeted therapeutics. Besides their direct activity against tumor cells, monoclonal antibodies and tyrosine kinase inhibitors (TKIs) also influence the antitumoral activity of immune...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20194693
更新日期：2019-09-22 00:00:00
abstract：:The TEAD family of transcription factors is necessary for developmental processes. The family members contain a TEA domain for the binding with DNA elements and a transactivation domain for the interaction with transcription coactivators. TEAD proteins are required for the participation of coactivators to transmit the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17010138
更新日期：2016-01-21 00:00:00
