Abstract:
:Proteogenomics enable the discovery of novel peptides (from unannotated genomic protein-coding loci) and single amino acid variant peptides (derived from single-nucleotide polymorphisms and mutations). Increasing the reliability of these identifications is crucial to ensure their usefulness for genome annotation and potential application as neoantigens in cancer immunotherapy. We here present integrated proteogenomics analysis workflow (IPAW), which combines peptide discovery, curation, and validation. IPAW includes the SpectrumAI tool for automated inspection of MS/MS spectra, eliminating false identifications of single-residue substitution peptides. We employ IPAW to analyze two proteomics data sets acquired from A431 cells and five normal human tissues using extended (pH range, 3-10) high-resolution isoelectric focusing (HiRIEF) pre-fractionation and TMT-based peptide quantitation. The IPAW results provide evidence for the translation of pseudogenes, lncRNAs, short ORFs, alternative ORFs, N-terminal extensions, and intronic sequences. Moreover, our quantitative analysis indicates that protein production from certain pseudogenes and lncRNAs is tissue specific.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Zhu Y,Orre LM,Johansson HJ,Huss M,Boekel J,Vesterlund M,Fernandez-Woodbridge A,Branca RMM,Lehtiö Jdoi
10.1038/s41467-018-03311-ysubject
Has Abstractpub_date
2018-03-02 00:00:00pages
903issue
1issn
2041-1723pii
10.1038/s41467-018-03311-yjournal_volume
9pub_type
杂志文章abstract::Some two-dimensional liquid systems are theoretically predicted to have an anomalous phase transition due to unique intermolecular interactions, for example the first-order transition between two-dimensional high-density water and low-density amorphous ice. However, it has never been experimentally observed, to the be...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02634-6
更新日期:2018-01-15 00:00:00
abstract::Interactions between metal nanoparticles and support materials can strongly influence the performance of catalysts. In particular, reducible oxidic supports can form suboxides that can decorate metal nanoparticles and enhance catalytic performance or block active sites. Therefore, tuning this metal-support interaction...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06903-w
更新日期:2018-10-26 00:00:00
abstract::Diverse entry inhibitors targeting the gp120 subunit of the HIV-1 envelope (Env) trimer have been developed including BMS-626529, also called temsavir, a prodrug version of which is currently in phase III clinical trials. Here we report the characterization of a panel of small-molecule inhibitors including BMS-818251,...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07851-1
更新日期:2019-01-03 00:00:00
abstract::Circulating tumor cells (CTCs) have become an established biomarker for prognosis in patients with various carcinomas. However, current ex vivo CTC isolation technologies rely on small blood volumes from a single venipuncture limiting the number of captured CTCs. This produces statistical variability and inaccurate re...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09439-9
更新日期:2019-04-01 00:00:00
abstract::Quantum triangular-lattice antiferromagnets are important prototype systems to investigate numerous phenomena of the geometrical frustration in condensed matter. Apart from highly unusual magnetic properties, they possess a rich phase diagram (ranging from an unfrustrated square lattice to a quantum spin liquid), yet ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09071-7
更新日期:2019-03-06 00:00:00
abstract::Graphene and carbon nanotubes are envisaged as suitable materials for the fabrication of the new generation of nanoelectronics. The controlled patterning of such nanostructures with metal nanoparticles is conditioned by the transfer between a recipient and the surface to pattern. Electromigration under the impact of a...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9071
更新日期:2015-08-28 00:00:00
abstract::Replacing sluggish oxygen evolution reaction (OER) with hydrazine oxidation reaction (HzOR) to produce hydrogen has been considered as a more energy-efficient strategy than water splitting. However, the relatively high cell voltage in two-electrode system and the required external electric power hinder its scalable ap...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15563-8
更新日期:2020-04-15 00:00:00
abstract::The insulin receptor (IR) and insulin-like growth factor-1 receptor (IGF1R) are highly related receptor tyrosine kinases with a disulfide-linked homodimeric architecture. Ligand binding to the receptor ectodomain triggers tyrosine autophosphorylation of the cytoplasmic domains, which stimulates catalytic activity and ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7406
更新日期:2015-03-11 00:00:00
abstract::Magnetic resonance imaging is an important technique for identifying different types of tissues in a body or spatial information about composite materials. Because temperature is a fundamental parameter reflecting the biological status of the body and individual tissues, it would be helpful to have temperature maps su...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12415
更新日期:2016-08-09 00:00:00
abstract::Photovoltaic devices based on metal halide perovskites are rapidly improving in efficiency. Once the Shockley-Queisser limit is reached, charge-carrier extraction will be limited only by radiative bimolecular recombination of electrons with holes. Yet, this fundamental process, and its link with material stoichiometry...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02670-2
更新日期:2018-01-18 00:00:00
abstract::Adolescence is a developmental period in which the mesolimbic dopaminergic "reward" circuitry of the brain, including the nucleus accumbens (NAc), undergoes significant plasticity. Dopamine D1 receptors (D1rs) in the NAc are critical for social behavior, but how these receptors are regulated during adolescence is not ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06118-z
更新日期:2018-09-25 00:00:00
abstract::Protein integration into biological membranes is a vital cellular event for all organisms. We previously reported an integration factor in the inner membrane of Escherichia coli, named MPIase (membrane protein integrase). Here we show that in contrast to previously identified integration factors that are proteins, MPI...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2267
更新日期:2012-01-01 00:00:00
abstract::Acoustic waves, capable of transmitting through optically opaque objects, have been widely used in biomedical imaging, industrial sensing and particle manipulation. High-fidelity wave front shaping is essential to further improve performance in these applications. An acoustic analog to the successful spatial light mod...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18347-2
更新日期:2020-09-10 00:00:00
abstract::This corrects the article DOI: 10.1038/ncomms15533. ...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/ncomms16129
更新日期:2017-07-04 00:00:00
abstract::Metal-organic frameworks (MOFs) are well known for their tunable structure and porosity. Many studies have shown they are promising for various important applications, for which their performance can be further enhanced by encapsulating functional species, such as luminescent guest molecules, within the frameworks. Al...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3717
更新日期:2013-01-01 00:00:00
abstract::Hyperpolarization via dynamic nuclear polarization (DNP) is pivotal for boosting magnetic resonance imaging (MRI) sensitivity and dissolution DNP can be used to perform in vivo real-time 13C MRI. The type of applications is however limited by the relatively fast decay time of the hyperpolarized spin state together wit...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15757
更新日期:2017-06-01 00:00:00
abstract::Paternal epigenetic inheritance is gaining attention for its growing medical relevance. However, the form in which paternal epigenetic information is transmitted to offspring and how it influences offspring development remain poorly understood. Here we show that in C. elegans, sperm-inherited chromatin states transmit...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09141-w
更新日期:2019-03-20 00:00:00
abstract::The primary productivity of the Southern Ocean ecosystem is limited by iron availability. Away from benthic and aeolian sources, iron reaches phytoplankton primarily when iron-rich subsurface waters enter the euphotic zone. Here, eddy-resolving physical/biogeochemical simulations of a seasonally-forced, open-Southern-...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14955-0
更新日期:2020-02-28 00:00:00
abstract::Selector devices are indispensable components of large-scale nonvolatile memory and neuromorphic array systems. Besides the conventional silicon transistor, two-terminal ovonic threshold switching device with much higher scalability is currently the most industrially favored selector technology. However, current ovoni...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18382-z
更新日期:2020-09-15 00:00:00
abstract::Recently, surface-enhanced Raman scattering nanoprobes have shown tremendous potential in oncological imaging owing to the high sensitivity and specificity of their fingerprint-like spectra. As current Raman scanners rely on a slow, point-by-point spectrum acquisition, there is an unmet need for faster imaging to cove...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09173-2
更新日期:2019-04-26 00:00:00
abstract::The current trends of crop yield improvements are not expected to meet the projected rise in demand. Genomic selection uses molecular markers and machine learning to identify superior genotypes with improved traits, such as growth. Plant growth directly depends on rates of metabolic reactions which transform nutrients...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16279-5
更新日期:2020-05-15 00:00:00
abstract::Somatic mutations contribute to tumorigenesis. Although these mutations occur in all proliferating cells, their accumulation under non-malignant conditions, such as in autoimmune disorders, has not been investigated. Here, we show that patients with newly diagnosed rheumatoid arthritis have expanded CD8+ T-cell clones...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15869
更新日期:2017-06-21 00:00:00
abstract::While intron retention (IR) is considered a widely conserved and distinct mechanism of gene expression control, its regulation is poorly understood. Here we show that DNA methylation directly regulates IR. We also find reduced occupancy of MeCP2 near the splice junctions of retained introns, mirroring the reduced DNA ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15134
更新日期:2017-05-08 00:00:00
abstract::Even a single-component liquid may have more than two liquid states. The transition between them is called a 'liquid-liquid transition' (LLT). Such LLTs have recently attracted considerable attention mainly because of the fundamental interest in the physical origin of this counter-intuitive phenomenon. In this study, ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1015
更新日期:2010-05-04 00:00:00
abstract::Upgrading C1 chemicals (for example, CO, CO/H2, MeOH and CO2) with C-C bond formation is essential for the synthesis of bulk chemicals. In general, these industrially important processes (for example, Fischer Tropsch) proceed at drastic reaction conditions (>250 °C; high pressure) and suffer from low selectivity, whic...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12075
更新日期:2016-07-05 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41467-020-16538-5
更新日期:2020-06-03 00:00:00
abstract::Inbreeding typically reduces fitness. Related partners may fail to reproduce and any inbred offspring may die early or fail to reproduce themselves. Here we show that inbreeding causes early death in the zebra finch Taeniopygia guttata, and among inbred individuals of the same inbreeding coefficient (F), those that di...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1870
更新日期:2012-05-29 00:00:00
abstract::Currently, there is no comprehensive framework to evaluate the evolutionary forces acting on genomic regions associated with human complex traits and contextualize the relationship between evolution and molecular function. Here, we develop an approach to test for signatures of diverse evolutionary forces on trait-asso...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17258-6
更新日期:2020-07-24 00:00:00
abstract::The removal of dying neurons by microglia has a key role during both development and in several diseases. To date, little is known about the cellular and molecular processes underlying neuronal engulfment in the brain. Here we took a live imaging approach to quantify neuronal cell death progression in embryonic zebraf...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5046
更新日期:2014-06-05 00:00:00
abstract::Liver cancer stem cells (CSCs) may contribute to the high rate of recurrence and heterogeneity of hepatocellular carcinoma (HCC). However, the biology of hepatic CSCs remains largely undefined. Through analysis of transcriptome microarray data, we identify a long noncoding RNA (lncRNA) called lncBRM, which is highly e...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13608
更新日期:2016-12-01 00:00:00