当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Immunologic targeting of CD30 eliminates tumourigenic human pluripotent stem cells, allowing safer clinical application of hiPSC-based cell therapy.

Immunologic targeting of CD30 eliminates tumourigenic human pluripotent stem cells, allowing safer clinical application of hiPSC-based cell therapy.

Abstract：

:Induced pluripotent stem cells (iPSCs) are promising candidate cells for cardiomyogenesis in the failing heart. However, teratoma/tumour formation originating from undifferentiated iPSCs contaminating the graft is a critical concern for clinical application. Here, we hypothesized that brentuximab vedotin, which targets CD30, induces apoptosis in tumourigenic cells, thus increasing the safety of iPSC therapy for heart failure. Flow cytometry analysis identified consistent expression of CD30 in undifferentiated human iPSCs. Addition of brentuximab vedotin in vitro for 72 h efficiently induced cell death in human iPSCs, associated with a significant increase in G2/M phase cells. Brentuximab vedotin significantly reduced Lin28 expression in cardiomyogenically differentiated human iPSCs. Transplantation of human iPSC-derived cardiomyocytes (CMs) without treatment into NOG mice consistently induced teratoma/tumour formation, with a substantial number of Ki-67-positive cells in the graft at 4 months post-transplant, whereas iPSC-derived CMs treated with brentuximab vedotin prior to the transplantation did not show teratoma/tumour formation, which was associated with absence of Ki-67-positive cells in the graft over the same period. These findings suggest that in vitro treatment with brentuximab vedotin, targeting the CD30-positive iPSC fraction, reduced tumourigenicity in human iPSC-derived CMs, potentially providing enhanced safety for iPSC-based cardiomyogenesis therapy in clinical scenarios.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Sougawa N,Miyagawa S,Fukushima S,Kawamura A,Yokoyama J,Ito E,Harada A,Okimoto K,Mochizuki-Oda N,Saito A,Sawa Y

doi

10.1038/s41598-018-21923-8

subject

Has Abstract

pub_date

2018-02-27 00:00:00

pages

3726

issue

1

issn

2045-2322

pii

10.1038/s41598-018-21923-8

journal_volume

8

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Unifying complexity and information.

    abstract：:Complex systems, arising in many contexts in the computer, life, social, and physical sciences, have not shared a generally-accepted complexity measure playing a fundamental role as the Shannon entropy H in statistical mechanics. Superficially-conflicting criteria of complexity measurement, i.e. complexity-randomness ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep01585

    authors： Ke DG

    更新日期：2013-01-01 00:00:00

  • Selection and validation of miR-1280 as a suitable endogenous normalizer for qRT-PCR Analysis of serum microRNA expression in Hepatocellular Carcinoma.

    abstract：:Normalization procedures for the qRT-PCR analysis of miRNA in biological samples are recommended to reduce the variability caused by pre-analytical factors. Since there is no universal standardized normalization strategy for miRNA qRT-PCR studies, we conducted a throughout study to evaluate a panel of small non-coding...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-59682-0

    authors： Pratama MY,Cavalletto L,Tiribelli C,Chemello L,Pascut D

    更新日期：2020-02-21 00:00:00

  • Host-guest complexes of imazalil with cucurbit[8]uril and β-cyclodextrin and their effect on plant pathogenic fungi.

    abstract：:We report the control of imazalil (IMZ) antifungal activity utilizing its non-covalent assembly with β-cyclodextrins (β-CD) and cucurbit[8]uril (CB8) macrocycles, as well as its stimuli-responsive disassembly with cadaverine. The NMR results are consistent with inclusion of a single IMZ molecule inside the cavities of...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21156-9

    authors： Al-Dubaili N,El-Tarabily K,Saleh N

    更新日期：2018-02-12 00:00:00

  • Transcriptomic analysis identifies novel genes and pathways for salt stress responses in Suaeda salsa leaves.

    abstract：:Salinity is a critical abiotic stress, which significantly impacts the agricultural yield worldwide. Identification of the molecular mechanisms underlying the salt tolerance in euhalophyte Suaeda salsa is conducive to the development of salt-resistant crops. In the present study, high-throughput RNA sequencing was per...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-61204-x

    authors： Zhang X,Yao Y,Li X,Zhang L,Fan S

    更新日期：2020-03-06 00:00:00

  • Maxwell Fisheye Lens Based Retrodirective Array.

    abstract：:A Maxwell fisheye lens using parallel plate index grading is presented in this study to develop a passive retrodirective antenna array. As a proof-of-concept a design frequency of 10 GHz was selected for fabrication and experiment. The design principals of the lens are discussed, which enables 85% energy flow at the d...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-52779-1

    authors： Abbasi MAB,Fusco VF

    更新日期：2019-11-07 00:00:00

  • Personalized structural image analysis in patients with temporal lobe epilepsy.

    abstract：:Volumetric and morphometric studies have demonstrated structural abnormalities related to chronic epilepsies on a cohort- and population-based level. On a single-patient level, specific patterns of atrophy or cortical reorganization may be widespread and heterogeneous but represent potential targets for further person...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-10707-1

    authors： Rummel C,Slavova N,Seiler A,Abela E,Hauf M,Burren Y,Weisstanner C,Vulliemoz S,Seeck M,Schindler K,Wiest R

    更新日期：2017-09-07 00:00:00

  • α-MSH and Foxc2 promote fatty acid oxidation through C/EBPβ negative transcription in mice adipose tissue.

    abstract：:Alpha melanocyte stimulating hormone (α-MSH) and Forkhead box C2 protein (Foxc2) enhance lipolysis in multiple tissues. However, their relationship in adipose fatty acid oxidation (FAO) remains unclear. Here, we demonstrated that α-MSH and Foxc2 increased palmitate oxidation to CO2 in white (WAT) and brown adipose tis...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep36661

    authors： Gan L,Liu Z,Chen Y,Dan Luo,Feng F,Liu G,Sun C

    更新日期：2016-11-07 00:00:00

  • Tracking EBV-encoded RNAs (EBERs) from the nucleus to the excreted exosomes of B-lymphocytes.

    abstract：:Epstein-Barr virus-encoded RNAs (EBER1 and EBER2) are two highly abundant, non-protein coding RNAs consistently expressed in all EBV infected cells, but their function remains poorly understood. Conventional in situ hybridization studies have indicated that these RNAs are present exclusively in the nucleus. We have re...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-33758-4

    authors： Ahmed W,Tariq S,Khan G

    更新日期：2018-10-18 00:00:00

  • Deriving dopaminergic neurons for clinical use. A practical approach.

    abstract：:New small molecules that regulate the step-wise differentiation of human pluripotent stem cells into dopaminergic neurons have been identified. The steroid, guggulsterone, was found to be the most effective inducer of neural stem cells into dopaminergic neurons. These neurons are extensively characterized and shown to...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep01463

    authors： Gonzalez R,Garitaonandia I,Abramihina T,Wambua GK,Ostrowska A,Brock M,Noskov A,Boscolo FS,Craw JS,Laurent LC,Snyder EY,Semechkin RA

    更新日期：2013-01-01 00:00:00

  • The effect of cold priming on the fitness of Arabidopsis thaliana accessions under natural and controlled conditions.

    abstract：:Priming improves an organism's performance upon a future stress. To test whether cold priming supports protection in spring and how it is affected by cold acclimation, we compared seven Arabidopsis accessions with different cold acclimation potentials in the field and in the greenhouse for growth, photosynthetic perfo...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep44055

    authors： Cvetkovic J,Müller K,Baier M

    更新日期：2017-03-09 00:00:00

  • Author Correction: SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-62994-w

    authors： Zhou Y,Sun W,Chen J,Tan H,Xiao Y,Li Q,Ji Q,Gao S,Chen L,Chen S,Zhang L,Chen W

    更新日期：2020-04-24 00:00:00

  • Genotyping-by-sequencing of three mapping populations for identification of candidate genomic regions for resistance to sterility mosaic disease in pigeonpea.

    abstract：:Sterility mosaic disease (SMD) is one of the serious production constraints that may lead to complete yield loss in pigeonpea. Three mapping populations including two recombinant inbred lines and one F2, were used for phenotyping for SMD resistance at two locations in three different years. Genotyping-by-sequencing ap...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-01535-4

    authors： Saxena RK,Kale SM,Kumar V,Parupali S,Joshi S,Singh V,Garg V,Das RR,Sharma M,Yamini KN,Ghanta A,Rathore A,Sameerkumar CV,Saxena KB,Varshney RK

    更新日期：2017-05-12 00:00:00

  • Modulation of salivary cytokines in response to alcohol, tobacco and caffeine consumption: a pilot study.

    abstract：:It has been shown that long-term stimulant consumption alters the biological and microbiological status of the oral cavity. We present a pilot study describing stimulant-specific oral immunomodulation in the oral cavity. Changes in salivary cytokine levels in response to long-term alcohol, tobacco and caffeine were id...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-35094-z

    authors： Sheth CC,López-Pedrajas RM,Jovani-Sancho MDM,González-Martínez R,Veses V

    更新日期：2018-11-12 00:00:00

  • Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp.

    abstract：:Rapid, sensitive, point-of-care diagnostics are critical for managing infectious diseases. Here we adapt the CRISPR-based SHERLOCK method to develop a rapid, accurate, single copy detection assay for White Spot Syndrome Virus, the most devastating virus impacting global shrimp aquaculture. Further, we combine paper ma...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56170-y

    authors： Sullivan TJ,Dhar AK,Cruz-Flores R,Bodnar AG

    更新日期：2019-12-23 00:00:00

  • Europium Luminescence: Electronic Densities and Superdelocalizabilities for a Unique Adjustment of Theoretical Intensity Parameters.

    abstract：:We advance the concept that the charge factors of the simple overlap model and the polarizabilities of Judd-Ofelt theory for the luminescence of europium complexes can be effectively and uniquely modeled by perturbation theory on the semiempirical electronic wave function of the complex. With only three adjustable con...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep13695

    authors： Dutra JD,Lima NB,Freire RO,Simas AM

    更新日期：2015-09-02 00:00:00

  • Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology.

    abstract：:Microtubule-associated protein 1B (MAP1B) is expressed predominantly during the early stages of development of the nervous system, where it regulates processes such as axonal guidance and elongation. Nevertheless, MAP1B expression in the brain persists in adult stages, where it participates in the regulation of the st...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep30069

    authors： Bodaleo FJ,Montenegro-Venegas C,Henríquez DR,Court FA,Gonzalez-Billault C

    更新日期：2016-07-18 00:00:00

  • Association of smoking and physical inactivity with MRI derived changes in cardiac function and structure in cardiovascular healthy subjects.

    abstract：:We aimed to investigate the association of smoking and physical exercise on ventricular function and structure, determined by cardiac magnetic resonance imaging (CMR), in subjects without known cardiovascular diseases. A total of 381 participants (median age 57 years) of the Cooperative Health Research in the Region o...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-54956-8

    authors： Schafnitzel A,Lorbeer R,Bayerl C,Patscheider H,Auweter SD,Meisinger C,Heier M,Ertl-Wagner B,Reiser M,Peters A,Bamberg F,Hetterich H

    更新日期：2019-12-09 00:00:00

  • Fluorescent and Magnetic Mesoporous Hybrid Material: A Chemical and Biological Nanosensor for Hg(2+) Ions.

    abstract：:We introduce "sense, track and separate" approach for the removal of Hg(2+) ion from aqueous media using highly ordered and magnetic mesoporous ferrosilicate nanocages functionalised with rhodamine fluorophore derivative. These functionalised materials offer both fluorescent and magnetic properties in a single system ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep21820

    authors： Suresh M,Anand C,Frith JE,Dhawale DS,Subramaniam VP,Strounina E,Sathish CI,Yamaura K,Cooper-White JJ,Vinu A

    更新日期：2016-02-25 00:00:00

  • Functional expression and pharmaceutical efficacy of cardiac-specific ion channels in human embryonic stem cell-derived cardiomyocytes.

    abstract：:Cardiomyocytes differentiated from human pluripotent stem cells provide promising tools for screening of cardiotoxic drugs. For evaluation of human pluripotent stem cell-derived cardiomyocytes for cardiotoxicity test, in the present study, human embryonic stem cells (hESCs) were differentiated to cardiomyocytes, follo...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-14198-y

    authors： Kim HS,Yoon JW,Li H,Jeong GO,Park JJ,Shin SE,Jang IH,Kim JH,Park WS

    更新日期：2017-10-23 00:00:00

  • Accuracy for diagnosis of periapical cystic lesions.

    abstract：:Clinical differentiation between cystic lesions of endodontic and non-endodontic origin is of importance because correct diagnosis may affect treatment decision making. Most radicular cysts are treated with conservative approaches and, therefore, are not surgically removed. The objective of this study was to determine...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71029-3

    authors： Tsesis I,Krepel G,Koren T,Rosen E,Kfir A

    更新日期：2020-08-25 00:00:00

  • Microgravity Induction of TRAIL Expression in Preosteoclast Cells Enhances Osteoclast Differentiation.

    abstract：:Evidence indicates that astronauts experience significant bone loss in space. We previously showed that simulated microgravity (μXg) using the NASA developed rotary cell culture system (RCCS) enhanced bone resorbing osteoclast (OCL) differentiation. However, the mechanism by which μXg increases OCL formation is unclea...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep25143

    authors： Sambandam Y,Baird KL,Stroebel M,Kowal E,Balasubramanian S,Reddy SV

    更新日期：2016-05-04 00:00:00

  • Modelling water levels of northwestern India in response to improved irrigation use efficiency.

    abstract：:The groundwater crisis in northwestern India is the result of over-exploitation of groundwater resources for irrigation. The Government of India has targeted a 20 percent improvement in irrigation groundwater use efficiency. In this perspective, and using a regional-scale calibrated and validated three-dimensional gro...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-70416-0

    authors： Shekhar S,Kumar S,Densmore AL,van Dijk WM,Sinha R,Kumar M,Joshi SK,Rai SP,Kumar D

    更新日期：2020-08-10 00:00:00

  • Acoustic Complexity of vocal fish communities: a field and controlled validation.

    abstract：:The Acoustic Complexity Index (ACI) is increasingly applied to the study of biodiversity in aquatic habitats. However, it remains unknown which types of acoustic information are highlighted by this index in underwater environments. This study explored the robustness of the ACI to fine variations in fish sound abundanc...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-28771-6

    authors： Bolgan M,Amorim MCP,Fonseca PJ,Di Iorio L,Parmentier E

    更新日期：2018-07-12 00:00:00

  • Author Correction: MC1R is dispensable for the proteinuria reducing and glomerular protective effect of melanocortin therapy.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-72744-7

    authors： Qiao Y,Berg AL,Wang P,Ge Y,Quan S,Zhou S,Wang H,Liu Z,Gong R

    更新日期：2020-09-21 00:00:00

  • Genetic variant in SWI/SNF complexes influences hepatocellular carcinoma risk: a new clue for the contribution of chromatin remodeling in carcinogenesis.

    abstract：:Chromatin remodeling has been newly established as an important cancer genome characterization and recent exome and whole-genome sequencing studies of hepatocellular carcinoma (HCC) showed that recurrent inactivating mutations in SWI/SNF subunits involved in the molecular basis of hepatocarcinogenesis. To test the hyp...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep04147

    authors： Zhong R,Liu L,Tian Y,Wang Y,Tian J,Zhu BB,Chen W,Qian JM,Zou L,Xiao M,Shen N,Yang H,Lou J,Qiu Q,Ke JT,Lu XH,Wang ZL,Song W,Zhang T,Li H,Wang L,Miao XP

    更新日期：2014-02-21 00:00:00

  • In vivo effect of opticin deficiency in cartilage in a surgically induced mouse model of osteoarthritis.

    abstract：:The SLRP opticin (OPTC) has been demonstrated to be produced and degraded in osteoarthritic (OA) human cartilage. Here, we investigated the in vivo effect of OPTC deficiency in OA cartilage. OA was induced in 10-week-old Optc -/- and Optc +/+ mice. Ten weeks post-surgery, cartilage was processed for histology and immu...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-18047-w

    authors： Farrán A,Valverde-Franco G,Tío L,Lussier B,Fahmi H,Pelletier JP,Bishop PN,Monfort J,Martel-Pelletier J

    更新日期：2018-01-11 00:00:00

  • The chemically driven phase transformation in a memristive abacus capable of calculating decimal fractions.

    abstract：:The accurate calculation of decimal fractions is still a challenge for the binary-coded computations that rely on von Neumann paradigm. Here, we report a kind of memristive abacus based on synaptic Ag-Ge-Se device, in which the memristive long-term potentiation and depression are caused by a chemically driven phase tr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep01230

    authors： Xu H,Xia Y,Yin K,Lu J,Yin Q,Yin J,Sun L,Liu Z

    更新日期：2013-01-01 00:00:00

  • Identification of Genes Associated with Smad3-dependent Renal Injury by RNA-seq-based Transcriptome Analysis.

    abstract：:Transforming growth factor-β/Smad3 signaling plays a critical role in the process of chronic kidney disease (CKD), but targeting Smad3 systematically may cause autoimmune disease by impairing immunity. In this study, we used whole-transcriptome RNA-sequencing to identify the differential gene expression profile, gene ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep17901

    authors： Zhou Q,Xiong Y,Huang XR,Tang P,Yu X,Lan HY

    更新日期：2015-12-09 00:00:00

  • Correlation of 360-degree Surface Mapping In Vivo Bioluminescence with Multi-Spectral Optoacoustic Tomography in Human Xenograft Tumor Models.

    abstract：:Pre-clinical monitoring of tumor growth and identification of distal metastasis requires a balance between accuracy and expediency. Bioluminescence imaging (BLI) is often used to track tumor growth but is primarily limited to planar 2-dimensional (2D) imaging. Consistent subject placement within a standard top-mounted...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21668-4

    authors： Brannen A,Eggert M,Nahrendorf M,Arnold R,Panizzi P

    更新日期：2018-02-20 00:00:00

  • Comprehensive analysis of the T-cell receptor beta chain gene in rhesus monkey by high throughput sequencing.

    abstract：:Profiling immune repertoires by high throughput sequencing enhances our understanding of immune system complexity and immune-related diseases in humans. Previously, cloning and Sanger sequencing identified limited numbers of T cell receptor (TCR) nucleotide sequences in rhesus monkeys, thus their full immune repertoir...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep10092

    authors： Li Z,Liu G,Tong Y,Zhang M,Xu Y,Qin L,Wang Z,Chen X,He J

    更新日期：2015-05-11 00:00:00