Abstract：
:NOD-like receptors (NLRs) are a family of intracellular pattern recognition receptors (PRRs) that play critical roles in innate immunity against pathogens infection. NLRC5, the largest member of NLR family, has been characterized as a regulator of innate immunity and MHC class I expression. Alternative splicing of NLRC5 is only reported in human and zebrafish. However, the function of NLRC5 isoforms in the innate immune responses remains unknown. In the present study, we report the functional characterization of zfNLRC5a and zfNLRC5d, two splicing isoforms of zebrafish NLRC5. zfNLRC5a and zfNLRC5d are generated by exon skipping, and whose alternative splicing sites exist in the region of LRRs. Fluorescence microscopy showed that zfNLRC5 isoforms were located throughout the entire cell including nuclear staining. The expression of zfNLRC5 isoform was inducible in response to bacterial and viral infections. During SVCV infection, the in vitro and in vivo studies found that zfNLRC5d overexpression increased protection against viral infection; however zfNLRC5a overexpression had no significant effect on antiviral activity. Interestingly, zfNLRC5 isoforms but not zfNLRC5 were involved in transcriptional regulation of TLRs and NF-κB signaling. Overexpression of zfNLRC5 isoforms also contributed to negative regulation of antibacterial immune response, with the decreased expression of nfkbiaa (IκBα). All together, these results firstly demonstrate the function of NLRC5 isoforms in antiviral and antibacterial immune responses both in vitro and in vivo.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Cao L,Wu XM,Hu YW,Xue NN,Nie P,Chang MXdoi
10.1016/j.dci.2018.02.013subject
Has Abstractpub_date
2018-07-01 00:00:00pages
153-163eissn
0145-305Xissn
1879-0089pii
S0145-305X(18)30027-2journal_volume
84pub_type
杂志文章abstract：:The enzyme phenoloxidase is a critical component of the immunological defence of invertebrates. Previously, we have shown that the activity of phenoloxidase in Sydney rock oysters (Saccostrea glomerata) correlates with the severity of QX disease outbreaks. The aetiological agent of QX disease is the opportunistic prot...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2007.06.006
更新日期：2008-01-01 00:00:00
abstract：:The activities of three enzymes, tyrosinase (monophenol oxidase, MPO), N-acetyltransferase (NAT), and tyrosine aminotransferase (TAT), were studied during eumelanotic encapsulation in host larvae of Drosophila melanogaster parasitized by the wasp, Leptopilina boulardi. At 24 h postinfection there was a tenfold increas...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(93)90021-h
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abstract：:The major milk whey proteins of the tammar wallaby (Macropus eugenii) have been identified over the total period of lactation using proteomic analysis techniques comprising two-dimensional electrophoresis, comparative image analysis, matrix assisted laser desorption ionisation mass spectrometry (MALDI MS), de novo pep...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2008.08.002
更新日期：2009-02-01 00:00:00
abstract：:Protein(s) reactive with N-acetyl-D-glucosamine (GlcNAc) was isolated from porcine nonimmune serum. The molecular weight of the purified protein was found to be mainly 40 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. The N-terminal 10 amino acid sequence of the purified pr...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2005.04.002
更新日期：2006-01-01 00:00:00
abstract：:The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway is one of the main pleiotropic cascades used to transmit information from extracellular receptors to the nucleus, which results in DNA transcription and expression of genes involved in immunity, proliferation, differentiat...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2017.12.019
更新日期：2018-04-01 00:00:00
abstract：:Penaeid shrimp, as an invertebrate, relies on the innate immunity to oppose the microbial invaders. Antimicrobial peptides (AMP) are an integral component of the innate immune system in most organisms and function as an early first line of defense against pathogens, but the knowledge about the pathways to regulate the...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2009.06.001
更新日期：2009-10-01 00:00:00
abstract：:We analyzed the effects of infection with Trichobilharzia ocellata on hemocytes of its snail host, Lymnaea stagnalis, and correlated them with successive stages of parasite development. Circulating hemocytes were studied at 0, 2, 4, 6, and 8 weeks post exposure (p.e.) with respect to cell number, distribution of subpo...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(91)90001-f
更新日期：1991-04-01 00:00:00
abstract：:We have characterized age-related thymic atrophy in the guinea pig, including identification of antibodies that allow immunohistochemical assessment of thymopoiesis. Age-related thymic atrophy in guinea pigs more closely resembles what occurs in humans histologically and in thymus weight, cellularity, and percent func...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(00)00071-9
更新日期：2001-06-01 00:00:00
abstract：:The first step in natural killer activity, target cell binding, was investigated ultrastructurally in order to identify presumptive cytotoxic cells. After incubation of unfractioned peripheral blood cells of the carp, Cyprinus carpio L., with HeLa tumour cells as targets, regular conjugate forming was observed. Target...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(88)90055-9
更新日期：1988-10-01 00:00:00
abstract：:β-defensins (BD) are the largest family of vertebrate defensins with potent antimicrobial, chemotactic and immune-regulatory activities. Four BD genes (BD1-4) have been cloned previously in rainbow trout but none have been reported in other salmonids. In this study seven BD genes (BD1a-b, 2-4, 5a-b) are characterised ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2019.103560
更新日期：2020-03-01 00:00:00
abstract：:The insect phenoloxidase (PO) cascade is known to be tightly regulated by serine proteases and serine protease inhibitors of the serpin family. As a key component of the insect immune system, it is also suspected to be inhibited by several endoparasitoid wasps, insects that develop inside other arthropods as hosts. Ho...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2008.11.013
更新日期：2009-05-01 00:00:00
abstract：:This study was performed to identify the expression patterns of the cathelicidin genes in a local chicken breed and to evaluate the antimicrobial activities of the cathelicidin peptides against pathogenic bacteria. This analysis revealed that the coding regions of CATH-1, -2, and -3 genes contain 447 bp, 465 bp, and 4...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.06.012
更新日期：2016-12-01 00:00:00
abstract：:High mobility group box protein 3 (HMGB3) regulates proliferation and inflammatory response in vertebrates. However, its functional roles in invertebrates are largely unknown. In this study, a HMGB3 homologue molecule was identified from Apostichopus japonicus (designated as AjHMGB3) by RACE approach. The full-length ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2017.07.026
更新日期：2017-12-01 00:00:00
abstract：:The skin mucus of the conger eel, Conger myriaster, contains galactose-specific agglutinins. Hemagglutinating activity is independent of divalent cations and is destroyed by heating at 50 degrees C for 15 min. The mucus agglutinins, named congerins, are a mixture of proteins with different electrical charges. Three of...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(88)90007-9
更新日期：1988-04-01 00:00:00
abstract：:Neonates of all species, including foals, are highly susceptible to infection, and neutrophils play a crucial role in innate immunity to infection. Evidence exists that neutrophils of neonatal foals are functionally deficient during the first weeks of life, including expression of cytokine genes such as IFNG. We hypot...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.06.012
更新日期：2018-10-01 00:00:00
abstract：:In recent years, several studies have demonstrated the existence of cytokine-like molecules in invertebrates, therefore suggesting that cytokines may have been conserved throughout evolution. In this study, we investigated the presence of immunoreactive TNF alpha (ir TNF alpha) in the gastropod mollusc Biomphalaria gl...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(94)90013-2
更新日期：1994-05-01 00:00:00
abstract：:Priming in invertebrates is the acquired capacity to better combat a pathogen due to a previous exposure to sub-lethal doses of the same organism. It is proposed to be functionally analogous to immune memory in vertebrates. Previous studies with Anopheles gambiae mosquitoes provide evidence that the inhibitory respons...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2015.05.004
更新日期：2015-10-01 00:00:00
abstract：:Age-equivalence shows that certain differentiative events and the onset of various types of immunocompetence follow the same sequence in all species as lymphocytes emerge during development. ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/s0145-305x(78)80003-2
更新日期：1978-07-01 00:00:00
abstract：:Junctional adhesion molecule (JAM), a subfamily of immunoglobulin superfamily (IgSF) with a couple of immunoglobulin domains, can act as regulator in homeostasis and inflammation of vertebrates. In the present study, a structural homolog of JAM-A (designated CgJAM-A-L) was screened out from oyster, Crassostrea gigas, ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2015.09.011
更新日期：2016-02-01 00:00:00
abstract：:Peroxinectin possesses the features of both peroxidase activity and adhesive property and plays important roles in innate immune system of crustaceans. In this study, the sequence of peroxinectin of Eriocheir sinensis (EsPX) was analyzed and its expression in response to exterior stimulation was detected in both in vi...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2015.02.016
更新日期：2015-07-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(82)90007-6
更新日期：1982-01-01 00:00:00
abstract：:We have studied the chicken's pineal gland by light and electron microscopy at 3 and 4 weeks of age. The results indicated that lymphocytes, plasma cells, secretory cells, basophil and eosinophil granulocytes enter the parenchyma and a new histologically defined tissue is formed which we call lymphopineal tissue. Insi...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(84)90068-5
更新日期：1984-10-01 00:00:00
abstract：:Toll/interleukin 1 receptor domain-containing adaptor protein (TIRAP) and toll/interleukin 1 receptor-domain-containing adapter-inducing interferon-β (TRIF) are crucial adaptors of signal transduction for the signaling pathways of toll-like receptors (TLRs). TIRAP and TRIF perform an essential function in an antimicro...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2020.103991
更新日期：2021-04-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2019.103467
更新日期：2020-01-01 00:00:00
abstract：:The effects of stress on immune activity and growth in early vertebrates have not been studied in detail. The present study used fine flounder (Paralichthys adspersus) skeletal muscle as a model to evaluate molecules involved in the stress response, including the glucocorticoid receptors, foxo1/3, and the target genes...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2015.06.007
更新日期：2015-11-01 00:00:00
abstract：:The study of the genetic regulation of infectious disease resistance depends on the availability of inbred lines or selection lines of the species under investigation. The small numbers of such lines of fish has limited the strategy in teleosts to studies of associations between disease and immune/health traits. Attem...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/s0145-305x(96)00032-8
更新日期：1996-11-01 00:00:00
abstract：:Extracellular vesicles are released from cells and participate in cell communication via transfer of protein and genetic cargo derived from the parent cells. EVs play roles in normal physiology and immunity and are also linked to various pathological processes. Peptidylarginine deiminases (PADs) are phylogenetically c...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2019.103397
更新日期：2019-10-01 00:00:00
abstract：:Variously colored abalone (Haliotis diversicolor Reeve, 1846), which is an important commercial aquatic species and has been widely cultured, frequently suffers from bacterial infection. Knowledge of the defense mechanism in this animal is still lacking and, so far few genes related to immune responses in abalones hav...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2008.04.007
更新日期：2008-01-01 00:00:00
abstract：:The relationship between plasma cells, macrophages, B and T cells, dendritic cells, and epithelium in the chicken Harderian gland have been studied by means of ultrastructural localization of the horseradish peroxidase following local immunization. After 5 d, peroxidase activity was found in vesicles located in macrop...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(92)90020-d
更新日期：1992-03-01 00:00:00
abstract：:Tumor vessels are known to be abnormal, with typically aberrant, leaky and disordered vessels. Here, we investigated whether polarized macrophage phenotypes are involved in tumor abnormal angiogenesis and what is its mechanism. We found that there was no difference in chemotaxis of polarized M1 and M2 macrophages to l...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.01.010
更新日期：2016-06-01 00:00:00
