In-situ visualization of sound-induced otolith motion using hard X-ray phase contrast imaging.

Abstract:

:Regarding the basics of ear structure-function relationships in fish, the actual motion of the solid otolith relative to the underlying sensory epithelium has rarely been investigated. Otolith motion has been characterized based on a few experimental studies and on approaches using mathematical modeling, which have yielded partially conflicting results. Those studies either predicted a simple back-and-forth motion of the otolith or a shape-dependent, more complex motion. Our study was designed to develop and test a new set-up to generate experimental data on fish otolith motion in-situ. Investigating the basic parameters of otolith motion requires an approach with high spatial and temporal resolution. We therefore used hard X-ray phase contrast imaging (XPCI). We compared two anatomically well-studied cichlid species, Steatocranus tinanti and Etroplus maculatus, which, among other features, differ in the 3D shape of their otoliths. In a water-filled tank, we presented a pure tone of 200 Hz to 1) isolated otoliths embedded in agarose serving as a simple model or 2) to a fish (otoliths in-situ). Our new set-up successfully visualized the motion of otoliths in-situ and therefore paves the way for future studies evaluating the principles of otolith motion.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Schulz-Mirbach T,Olbinado M,Rack A,Mittone A,Bravin A,Melzer RR,Ladich F,Heß M

doi

10.1038/s41598-018-21367-0

subject

Has Abstract

pub_date

2018-02-15 00:00:00

pages

3121

issue

1

issn

2045-2322

pii

10.1038/s41598-018-21367-0

journal_volume

8

pub_type

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