Abstract：
AIM:Antimicrobial peptides (AMPs) are one of the most active components of innate immunity and have characteristics that could place them at the heart of the pathogenesis of periodontal disease. This study investigated differences in the expression of AMP coding genes obtained using a simple harvesting technique, gingival smear, between two groups of patients: chronic periodontitis subjects versus healthy ones. MATERIALS AND METHODS:Twenty-three patients were enrolled in two groups: 12 were diagnosed with moderate or severe generalized chronic periodontitis, and 11 were diagnosed as clinically healthy. Gingival smears were retrieved and studied using reverse transcription-quantitative PCR (RT-qPCR) after mRNA purification. RESULTS:Fifteen gene expressions were obtained using real-time RT-qPCR. Three AMP genes, histatin 3 (HTN3), α-defensin 4 (DEFA4) and lysozyme C (LYZ), presented different expression levels in periodontitis patients compared with healthy subjects. The relative expression level of DEFA4 appeared to be a protective factor against periodontitis. CONCLUSION:Gingival smears studied by RT-qPCR may be used to assess the expression of AMPs coding genes. A lack of expression of DEFA4 could be a potential indicator of periodontitis status.
journal_name
J Clin Periodontoljournal_title
Journal of clinical periodontologyauthors
Jourdain ML,Pierrard L,Kanagaratnam L,Velard F,Sergheraert J,Lefèvre B,Gangloff SC,Braux Jdoi
10.1111/jcpe.12879subject
Has Abstractpub_date
2018-05-01 00:00:00pages
524-537issue
5eissn
0303-6979issn
1600-051Xjournal_volume
45pub_type
杂志文章abstract：AIM:Matrix metalloproteinases (MMPs) play a key role in the tissue destruction characteristic of chronic periodontitis. The purpose of this study was to investigate the association of MMP and TIMP polymorphisms with chronic periodontitis in two populations. MATERIAL AND METHODS:A total of 34 polymorphisms spanning 12 ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2012.01902.x
更新日期：2012-08-01 00:00:00
abstract：AIM:Reports regarding prevalence of peri-implant diseases show widely varying prevalence rates, which can be explained partially by variable diagnostic criteria adopted. Furthermore, several different factors have been associated with peri-implant diseases. Hence, the aim of this cross-sectional study is to (a) determi...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.13111
更新日期：2019-05-01 00:00:00
abstract：BACKGROUND:The aim of this Systematic Review (SR) was to assess the clinical efficacy of periodontal plastic surgery procedures in the treatment of localized gingival recessions (Rec) with or without inter-dental clinical attachment loss (iCAL). MATERIAL AND METHODS:Electronic and hand searches were performed to ident...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,评审
doi：10.1111/jcpe.12182
更新日期：2014-04-01 00:00:00
abstract：:Experiments have been performed in beagle dogs in attempts to evaluate the effect of orthodontic- and jiggling-type trauma on the supporting structures of premolars. The results reported have unanimously demonstrated that in situations where orthodontic or jiggling forces were inflicted on teeth with a normal periodon...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,评审
doi：10.1111/j.1600-051x.1986.tb01427.x
更新日期：1986-11-01 00:00:00
abstract：:22 females and 3 males with primary hypogammaglobulinaemia (n = 6) or IgG subclass deficiencies with (n = 3) or without (n = 16) concomitant IgA deficiency were examined for periodontal disease and dental caries. Only 1 patient showed more tooth loss than that found in the normal Swedish population. 1 patient demonstr...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1993.tb01752.x
更新日期：1993-01-01 00:00:00
abstract：:A videobased computer assisted densitometric image analysis (CADIA) system to quantify alveolar bone density changes on standardized dental radiographs was tested. An algorithm was used for grey level correction of a subsequent image to the baseline image. Quantitative information regarding positive and/or negative gr...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1988.tb01551.x
更新日期：1988-01-01 00:00:00
abstract：AIM:To describe the prevalence, severity and extension of clinical attachment loss (CAL) and to study the predictors in 15- to 19-year-old adolescents from high schools in the Latin America. MATERIALS AND METHODS:A cross-sectional, epidemiological study was performed. The sample included 1070 high school adolescents 1...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12452
更新日期：2015-10-01 00:00:00
abstract：AIM:The aim of this study was to assess the use of a micro-brush to remove plaque deposits from subgingival, periodontally involved root surfaces in vivo. METHODS:30 periodontally involved teeth requiring extraction for periodontal or prosthetic reasons in 26 adult patients were utilised. For inclusion, teeth had to d...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.2001.028009820.x
更新日期：2001-09-01 00:00:00
abstract：:13 patients with untreated adult periodontitis (AP) were compared to 8 subjects free of periodontal disease (H) with respect to plaque index (PlI), gingival index (GI), probing depth (PD) and differential counts of subgingival bacterial morphotypes from a pooled sample of 6 surfaces with the greatest probing depth. Se...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1986.tb00878.x
更新日期：1986-09-01 00:00:00
abstract：AIM:The goal of this study was to evaluate if dental pulp stem cells (DPSCs) delivered into intrabony defects in a collagen scaffold would enhance the clinical and radiographic parameters of periodontal regeneration. MATERIALS AND METHODS:In this randomized controlled trial, 29 chronic periodontitis patients presentin...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,随机对照试验
doi：10.1111/jcpe.12931
更新日期：2018-07-01 00:00:00
abstract：:The present review concerns the effect on plaque and gingivitis of some antibacterial dentifrices. The difficulties of including cationic agents in conventional dentifrice formulations are briefly discussed. The focus is on 2 recently introduced dentifrices with triclosan as the active ingredient. One aims at providin...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,评审
doi：10.1111/j.1600-051x.1991.tb02318.x
更新日期：1991-07-01 00:00:00
abstract：AIM:The primary goal was to compare the amount of keratinized tissue width (KTW) gain after free gingival graft (FGG) procedures around implants and teeth after 6 and 12 months of healing. MATERIALS AND METHODS:Patients with mucogingival defects (<2 mm of KT) around teeth and implants underwent a gingival augmentation...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.13394
更新日期：2021-02-01 00:00:00
abstract：AIM:To evaluate the effect of the prebiotic (PREB) mannan oligosaccharide (MOS) on the progression of the experimental periodontitis (EP) and intestinal morphology in rats. MATERIALS AND METHODS:Forty rats were randomly allocated into groups (n = 10): C (control), PREB, EP and EP-PREB. Groups PREB and EP-PREB received...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12987
更新日期：2018-09-01 00:00:00
abstract：:11 volunteer subjects with advanced chronic periodontitis participated in a 1-year longitudinal clinical and microbiological study. Subgingival plaque was collected at each of 7 visits from 148 pre-selected sites in the left jaw quadrants (test sites) and on the first and last visits, only from 117 sites in the left j...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1988.tb01592.x
更新日期：1988-05-01 00:00:00
abstract：:During a clinical trial for evaluating the antiplaque effect of two flavoured chlorhexidine rinses, the observation was made that bleeding after gentle massage of the gingival margin occurred more often after chemical than after mechanical oral hygiene measures. In order to ascertain the validity of this unexpected ob...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1982.tb02100.x
更新日期：1982-07-01 00:00:00
abstract：AIM:The aim of this study was to test whether or not soft tissue augmentation with a collagen matrix (VCMX) leads to a similar increase in ridge width around dental implants compared to the use of an autogenous subepithelial connective tissue graft (SCTG). MATERIALS AND METHODS:In 12 dogs, immediate dental implants we...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12635
更新日期：2017-02-01 00:00:00
abstract：:The oral hygiene of habitual chewing stick and toothbrush users who participated in an oral health education (OHE) programme in schools was evaluated. The study comprised all chewing stick users (n = 45) in standard 4 in 8 randomly selected experimental schools and all chewing stick users (n = 17) in standard 4 in 4 r...
journal_title：Journal of clinical periodontology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1111/j.1600-051x.1992.tb01157.x
更新日期：1992-08-01 00:00:00
abstract：AIM:The purpose of this study was to assess the initiation and progression of periodontal disease during adult life. MATERIALS AND METHODS:In a 26-year longitudinal investigation of the initiation and progression of chronic periodontitis that started in 1969 and included 565 men of Norwegian middle class, 223 who had ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.2003.00399.x
更新日期：2003-10-01 00:00:00
abstract：AIM:Air-polishing devices (APDs) are highly effective in removing plaque and extrinsic staining. Their application on root surfaces, however, may result in clinically relevant substance removal, limiting the use in patients with periodontitis, where denuded root surfaces are frequently found. Therefore, the purpose of ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.2003.300204.x
更新日期：2003-02-01 00:00:00
abstract：:The objective of this study was to evaluate the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) concentration on regeneration of alveolar bone and cementum, and on associated root resorption and ankylosis. Contralateral, critical size, supra-alveolar, periodontal defects were surgically produced and...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.1999.260610.x
更新日期：1999-06-01 00:00:00
abstract：:The present clinical trial was performed to study the effect of systemic administration of metronidazole and amoxicillin as an adjunct to mechanical therapy in patients with advanced periodontal disease. 16 individuals, 10 female and 6 male, aged 35-58 years, with advanced periodontal disease were recruited. A baselin...
journal_title：Journal of clinical periodontology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1111/j.1600-051x.1998.tb02455.x
更新日期：1998-05-01 00:00:00
abstract：AIMS:To compare a monotherapy of Er:YAG laser debridement (ERL), wavelength 2940 nm, with mechanical scaling and root planing (SRP) for the treatment of chronic periodontitis using clinical and patient-centred outcomes. METHODS:Twenty-eight participants had two randomly assigned quadrants treated with ERL and two with...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-051X.2012.01873.x
更新日期：2012-06-01 00:00:00
abstract：AIM:The study was set to evaluate the validity of subjective jaw bone quality assessment. MATERIALS AND METHODS:A total of 298 patients (198 females, mean age 56.4) were treated with oral implants at the Periodontology Department at the University Hospital of KUL. A total of 761 TiUnite implants have been installed. S...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2007.01047.x
更新日期：2007-04-01 00:00:00
abstract：AIM:To present the 6-year results of a randomized-controlled clinical trial evaluating guided tissue regeneration (GTR) combined with or without deproteinized bovine bone mineral (DBBM) in intrabony defects. MATERIAL & METHODS:In each of 45 patients, one defect was treated with GTR combined with DBBM hydrated in salin...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-051X.2009.01520.x
更新日期：2010-02-01 00:00:00
abstract：OBJECTIVES:Although the direct cause for periodontitis is oral bacterial infection, its progression depends upon genetic and environmental factors. Smoking, one of the environmental factors, is a risk factor for the development and severity of periodontitis. Therefore, individual susceptibility to periodontitis may be ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2004.00587.x
更新日期：2004-11-01 00:00:00
abstract：:A previous study demonstrated structural differences in the junctional epithelium between juvenile and adult dogs. In juveniles the junctional epithelium showed some resemblances to the oral gingival epithelium, and a cuticular structure at the dento-gingival junction was of particular interest. The oral gingival epit...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1979.tb01941.x
更新日期：1979-12-01 00:00:00
abstract：AIM:Standard partial-mouth estimators of chronic periodontitis (CP) that define an individual's disease status solely in terms of selected sites underestimate prevalence. This study proposes an improved prevalence estimator based on randomly sampled sites and evaluates its accuracy in a well-characterized population co...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12656
更新日期：2017-03-01 00:00:00
abstract：AIM:This study evaluated the levels of sclerostin (SOST) and Dickkopf (DKK)-1 in the chronic periodontitis (CP) associated with type 2 diabetes (DM) and/or smoking. Relationships between SOST, DDK1, RANKL, OPG, IL-1ß, IL-6 and TNF-α, and pathogens were assessed. MATERIAL AND METHODS:The study population included non-d...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12854
更新日期：2018-03-01 00:00:00
abstract：:Early plaque growth and plaque topography were studied in 15 young adults with a healthy periodontium during a 4-day period of no oral hygiene. After perfect tooth-cleaning, the recolonisation of the buccal surfaces of 48 teeth (front teeth and premolars) was recorded at the start and after 6, 12, 24, 30, 36, 48, 54, ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1989.tb01655.x
更新日期：1989-05-01 00:00:00
abstract：BACKGROUND:It was demonstrated that a marginal defect of about 1 mm between the bone wall and the metal surface after implant installation can heal with a high degree of bone fill and osseointegration. OBJECTIVE:The aim of the present animal experiment was to study bone healing at implant sites with hard tissue defect...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2004.00502.x
更新日期：2004-04-01 00:00:00
