Abstract：
:We use super-resolution interferometric photoactivation and localization microscopy (iPALM) and a constrained photoactivatable fluorescent protein integrin fusion to measure the displacement of the head of integrin lymphocyte function-associated 1 (LFA-1) resulting from integrin conformational change on the cell surface. We demonstrate that the distance of the LFA-1 head increases substantially between basal and ligand-engaged conformations, which can only be explained at the molecular level by integrin extension. We further demonstrate that one class of integrin antagonist maintains the bent conformation, while another antagonist class induces extension. Our molecular scale measurements on cell-surface LFA-1 are in excellent agreement with distances derived from crystallographic and electron microscopy structures of bent and extended integrins. Our distance measurements are also in excellent agreement with a previous model of LFA-1 bound to ICAM-1 derived from the orientation of LFA-1 on the cell surface measured using fluorescence polarization microscopy.
journal_name
Cell Repjournal_title
Cell reportsauthors
Moore TI,Aaron J,Chew TL,Springer TAdoi
10.1016/j.celrep.2018.01.062subject
Has Abstractpub_date
2018-02-13 00:00:00pages
1903-1912issue
7issn
2211-1247pii
S2211-1247(18)30111-6journal_volume
22pub_type
杂志文章相关文献
Cell Reports文献大全abstract：:Chronic itch or pruritus is a debilitating disorder that is refractory to conventional anti-histamine treatment. Kappa opioid receptor (KOR) agonists have been used to treat chronic itch, but the underlying mechanism remains elusive. Here, we find that KOR and gastrin-releasing peptide receptor (GRPR) overlap in the s...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.03.087
更新日期：2018-04-17 00:00:00
abstract：:A new influenza-like virus genome (H17N10) was recently discovered in bats and offers a new perspective about the origin and evolution of influenza viruses. The viral envelope glycoprotein hemagglutinin (HA) is responsible for influenza virus receptor binding, fusion, and entry into the cell; therefore, the structure ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.01.025
更新日期：2013-03-28 00:00:00
abstract：:Either caspase-1 or caspase-11 can cleave gasdermin D to cause pyroptosis, eliminating intracellular replication niches. We previously showed that macrophages detect Burkholderia thailandensis via NLRC4, triggering the release of interleukin (IL)-18 and driving an essential interferon (IFN)-γ response that primes casp...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107967
更新日期：2020-07-28 00:00:00
abstract：:The facilitates chromatin transcription (FACT) complex is involved in chromatin remodeling during transcription, replication, and DNA repair. FACT was previously considered to be ubiquitously expressed and not associated with any disease. However, we discovered that FACT is the target of a class of anticancer compound...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.06.013
更新日期：2013-07-11 00:00:00
abstract：:Although our understanding of centromere maintenance, marked by the histone H3 variant CenH3(CENP-A) in most eukaryotes, has progressed, the mechanism underlying the de novo formation of centromeres remains unclear. We used a synthetic system to dissect how CenH3(CENP-A) contributes to the accumulation of CENP-C and C...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.03.013
更新日期：2015-04-07 00:00:00
abstract：:Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone involved in nutrient homeostasis. GIP receptor (GIPR) is constitutively internalized and returned to the plasma membrane, atypical behavior for a G-protein-coupled receptor (GPCR). GIP promotes GIPR downregulation from the plasma membrane by inh...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.11.050
更新日期：2016-12-13 00:00:00
abstract：:Intracellular transport and homeostasis of the endomembrane system in eukaryotic cells depend on the formation and fusion of vesicular carriers. Coat protein complex (COP) II vesicles export newly synthesized secretory proteins from the endoplasmic reticulum (ER), whereas COPI vesicles facilitate traffic from the Golg...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.12.041
更新日期：2019-01-02 00:00:00
abstract：:Transient receptor potential (TRP) channels are regulated by diverse stimuli comprising thermal, chemical, and mechanical modalities. They are also commonly regulated by phosphatidylinositol-4,5-bisphosphate (PIP2), with underlying mechanisms largely unknown. We here revealed an intramolecular interaction of the TRPP3...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.01.042
更新日期：2018-02-06 00:00:00
abstract：:Experience-dependent synapse refinement is essential for functional optimization of neural circuits. However, how sensory experience sculpts excitatory synaptic transmission is poorly understood. Here, we show that despite substantial remodeling of synaptic connectivity, AMPAR-mediated synaptic transmission remains at...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.12.084
更新日期：2017-01-24 00:00:00
abstract：:Macrophages exist predominantly in two distinct states, G0 and a G1-like state that is accompanied by phosphorylation of SAMHD1 at T592. Here, we demonstrate that Toll-like receptor 4 (TLR4) activation can potently induce G0 arrest and SAMHD1 antiretroviral activity by an interferon (IFN)-independent pathway. This pat...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.03.008
更新日期：2020-03-24 00:00:00
abstract：:The segregation of chromosomes is a critical step during cell division. This process is driven by the elongation of spindle microtubules and is tightly regulated by checkpoint mechanisms. It is unknown whether microtubules affect checkpoint responses as passive contributors or active regulators of the process. We show...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.02.051
更新日期：2019-03-12 00:00:00
abstract：:Neurodegenerative disorders are a major public health problem because of the high frequency of these diseases. Genome editing with the CRISPR/Cas9 system is making it possible to modify the sequence of genes linked to these disorders. We designed the KamiCas9 self-inactivating editing system to achieve transient expre...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.08.075
更新日期：2017-09-19 00:00:00
abstract：:Intratumoral (IT) STING activation results in tumor regression in preclinical models, yet factors dictating the balance between innate and adaptive anti-tumor immunity are unclear. Here, clinical candidate STING agonist ADU-S100 (S100) is used in an IT dosing regimen optimized for adaptive immunity to uncover requirem...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.11.047
更新日期：2018-12-11 00:00:00
abstract：:Chronic low-grade inflammation and increased serum levels of the cytokine IL-6 accompany obesity. For brain-produced IL-6, the mechanisms by which it controls energy balance and its role in obesity remain unclear. Here, we show that brain-produced IL-6 is decreased in obese mice and rats in a neuroanatomically and sex...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.02.044
更新日期：2019-03-12 00:00:00
abstract：:Generation of insulin-secreting β cells in vitro is a promising approach for diabetes cell therapy. Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are differentiated to β cells (SC-β cells) and mature to undergo glucose-stimulated insulin secretion, but molecular regulation of thi...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107687
更新日期：2020-05-26 00:00:00
abstract：:TRBP functions as both a Dicer cofactor and a PKR inhibitor. However, the role of TRBP in microRNA (miRNA) biogenesis is controversial and its regulation of PKR in mitosis remains unexplored. Here, we generate TRBP knockout cells and find altered Dicer-processing sites in a subset of miRNAs but no effect on Dicer stab...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.09.039
更新日期：2014-11-06 00:00:00
abstract：:Embryonic stem cells (ESCs) exhibit high levels of ribosomal RNA (rRNA) transcription and ribosome biogenesis. Here, we reveal an unexpected role for an essential DEAD-box helicase, DDX18, in antagonizing the polycomb repressive complex 2 (PRC2) to prevent deposition of the repressive H3K27me3 mark onto rDNA in plurip...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.12.021
更新日期：2020-01-07 00:00:00
abstract：:While interphase mitochondria associate with microtubules, mitotic mitochondria dissociate from spindle microtubules and localize in the cell periphery. Here, we show that this redistribution is not mediated by mitochondrial active transport or tethering to the cytoskeleton. Instead, kinesin and dynein, which link mit...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.07.055
更新日期：2016-08-23 00:00:00
abstract：:A major challenge in biology is determining how evolutionarily novel characters originate; however, mechanistic explanations for the origin of new characters are almost completely unknown. The evolution of pregnancy is an excellent system in which to study the origin of novelties because mammals preserve stages in the...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.12.052
更新日期：2015-02-03 00:00:00
abstract：:Human rhinovirus (HRV) causes upper respiratory infections and asthma exacerbations. We screened multiple orthologous RNAi reagents and identified host proteins that modulate HRV replication. Here, we show that RNASEK, a transmembrane protein, was needed for the replication of HRV, influenza A virus, and dengue virus....
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.06.076
更新日期：2015-08-04 00:00:00
abstract：:Lysosomes play a critical role in maintenance of the integrity of neuronal function, and mutations in genes that contribute to lysosome formation, transport, and activity are associated with neurodegenerative disorders. Recently, the multisubunit complex, BLOC-one-related complex (BORC), has been shown to be involved ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.06.118
更新日期：2018-07-31 00:00:00
abstract：:The enteroinvasive bacterium Shigella flexneri forces its uptake into non-phagocytic host cells through the translocation of T3SS effectors that subvert the actin cytoskeleton. Here, we report de novo actin polymerization after cellular entry around the bacterium-containing vacuole (BCV) leading to the formation of a ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107638
更新日期：2020-05-12 00:00:00
abstract：:Insulin stimulates glucose uptake through the membrane translocation of GLUT4 and GLUT1. Peroxisome proliferator-activated receptor γ (PPARγ) enhances insulin sensitivity. Here, we demonstrate that insulin stimulates GLUT4 and GLUT1 translocation, and glucose uptake, by activating the signaling pathway involving nicot...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2012.10.018
更新日期：2012-12-27 00:00:00
abstract：:Genetically encoded effectors are important tools for probing cellular function in living animals, but improved methods for directing their expression to specific cell types are required. Here, we introduce a simple, versatile method for achieving cell-type-specific expression of transgenes that leverages the untapped...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.01.059
更新日期：2015-03-03 00:00:00
abstract：:Billions of cells undergo apoptosis daily and often fragment into small, membrane-bound extracellular vesicles termed apoptotic bodies (ApoBDs). We demonstrate that apoptotic monocytes undergo a highly coordinated disassembly process and form long, beaded protrusions (coined as beaded apoptopodia), which fragment to r...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.10.014
更新日期：2019-11-12 00:00:00
abstract：:FBXL2 targets IP3R3 for ubiquitin-mediated degradation to limit Ca2+ flux to mitochondria and, consequently, apoptosis. Efficient replication of hepatitis C virus (HCV) requires geranylgeranylation of FBXL2. Here, we show that the viral protein NS5A forms a trimeric complex with IP3R3 and FBXL2, unmasking IP3R3's degr...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.09.088
更新日期：2018-10-23 00:00:00
abstract：:The ability of cells to stably maintain their fate is governed by specific transcription regulators. Here, we show that the Scalloped (Sd) and Nervous fingers-1 (Nerfin-1) transcription factors physically and functionally interact to maintain medulla neuron fate in the Drosophila melanogaster CNS. Using Targeted DamID...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.10.038
更新日期：2018-11-06 00:00:00
abstract：:Organisms need to assess their nutritional state and adapt their digestive capacity to the demands for various nutrients. Modulation of digestive enzyme production represents a rational step to regulate nutriment uptake. However, the role of digestion in nutrient homeostasis has been largely neglected. In this study, ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.08.064
更新日期：2014-10-09 00:00:00
abstract：:Parsing diverse nerve cells into biological types is necessary for understanding neural circuit organization. Morphology is an intuitive criterion for neuronal classification and a proxy of connectivity, but morphological diversity and variability often preclude resolving the granularity of neuron types. Combining gen...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.02.040
更新日期：2019-03-12 00:00:00
abstract：:The flat bones of the skull are densely innervated during development, but little is known regarding their role during repair. We describe a neurotrophic mechanism that directs sensory nerve transit in the mouse calvaria. Patent cranial suture mesenchyme represents an NGF (nerve growth factor)-rich domain, in which se...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107696
更新日期：2020-05-26 00:00:00
