Abstract:
:Cataract is the leading cause of blindness with an estimated 16 million people affected worldwide. d-galactose (d-gal) is a reducing sugar that widely distributed in foodstuffs, and studies show that d-gal could promote cataract formation by damaging nature lens epithelial cells (LECs). However, the underlying mechanism is unclear. In our present study, d-gal resulted in premature senescence of LECs, which was confirmed by determining the β-galactosidase activity, cell proliferative potential and cell cycle distribution, though apoptosis of LECs was not observed. We also verified that d-gal induced the impairment of autophagy flux by measuring the expression of LC3II and P62. Meanwhile, we found that d-gal induced mitochondrial dysfunctions of LECs through increasing reactive oxygen species (ROS), reducing ATP synthesis and mitochondrial potential (MMP), enhancing the concentration of cytoplasm Ca2+ and permeability transition pore (mPTP) opening. Metformin, as a potential anti-aging agent, suppressed the senescence of LECs by restoring autophagy flux and mitochondria functions. Nevertheless, the antioxidant N-acetylcysteine (NAC) scavenged ROS significantly but was not efficient in preventing LECs from premature senescence. Our data suggests that restoring autophagy activity and improving mitochondrial functions may be a potential strategy for the prevention of LECs senescence-related cataract.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Xu Y,Li Y,Ma L,Xin G,Wei Z,Zeng Z,Xing Z,Li S,Niu H,Huang Wdoi
10.1016/j.toxlet.2018.02.001subject
Has Abstractpub_date
2018-06-01 00:00:00pages
99-106eissn
0378-4274issn
1879-3169pii
S0378-4274(18)30039-0journal_volume
289pub_type
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journal_title:Toxicology letters
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journal_title:Toxicology letters
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journal_title:Toxicology letters
pub_type: 杂志文章,评审
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journal_title:Toxicology letters
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doi:10.1016/0378-4274(87)90168-8
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journal_title:Toxicology letters
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journal_title:Toxicology letters
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journal_title:Toxicology letters
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journal_title:Toxicology letters
pub_type: 杂志文章
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journal_title:Toxicology letters
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doi:10.1016/0378-4274(88)90034-3
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2013.02.003
更新日期:2013-04-26 00:00:00
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2017.11.016
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journal_title:Toxicology letters
pub_type: 杂志文章
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2017.09.013
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2005.03.002
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/s0378-4274(03)00071-7
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journal_title:Toxicology letters
pub_type: 杂志文章,评审
doi:10.1016/j.toxlet.2017.10.003
更新日期:2018-01-05 00:00:00
abstract::Triphenyltin (TPT) is an organotin compound and may be an endocrine disruptor, impairing the male reproductive system. However, the effect of short-term TPT exposure on stem Leydig cell regeneration later on remains unknown. Here, we show that TPT affects stem Leydig cell regeneration in the adult rat testis. Adult ma...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2019.02.010
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2019.11.028
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journal_title:Toxicology letters
pub_type: 杂志文章
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2020.08.002
更新日期:2020-10-15 00:00:00
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2012.03.002
更新日期:2012-06-01 00:00:00
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(83)90049-8
更新日期:1983-06-01 00:00:00
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(83)90072-3
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/0378-4274(95)80008-2
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journal_title:Toxicology letters
pub_type: 杂志文章,评审
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2010.06.015
更新日期:2010-10-05 00:00:00
abstract::Benzodiazepines have wide-spread used in pharmacotherapy for their anxiolytic, myorelaxant, hypnotic, amnesic and anticonvulsive properties. Despite benzodiazepines are used in clinics over 50 years, they have not been surprisingly tested for capability to induce major drug-metabolizing cytochromes P450. In the curren...
journal_title:Toxicology letters
pub_type: 杂志文章
doi:10.1016/j.toxlet.2010.01.004
更新日期:2010-03-15 00:00:00
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journal_title:Toxicology letters
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00