Abstract：
:Staphylococcus aureus infection is a rising public health care threat. S. aureus is believed to have elaborate regulatory networks that orchestrate its virulence. Despite its importance, the systematic understanding of the transcriptional landscape of S. aureus is limited. Here, we describe the primary transcriptome landscape of an epidemic USA300 isolate of community-acquired methicillin-resistant S. aureus. We experimentally determined 1,861 transcription start sites with their principal promoter elements, including well-conserved -35 and -10 elements and weakly conserved -16 element and 5' untranslated regions containing AG-rich Shine-Dalgarno sequence. In addition, we identified 225 genes whose transcription was initiated from multiple transcription start sites, suggesting potential regulatory functions at transcription level. Along with the transcription unit architecture derived by integrating the primary transcriptome analysis with operon prediction, the measurement of differential gene expression revealed the regulatory framework of the virulence regulator Agr, the SarA-family transcriptional regulators, and β-lactam resistance regulators. Interestingly, we observed a complex interplay between virulence regulation, β-lactam resistance, and metabolism, suggesting a possible tradeoff between pathogenesis and drug resistance in the USA300 strain. Our results provide platform resource for the location of transcription initiation and an in-depth understanding of transcriptional regulation of pathogenesis, virulence, and antibiotic resistance in S. aureus.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Choe D,Szubin R,Dahesh S,Cho S,Nizet V,Palsson B,Cho BKdoi
10.1038/s41598-018-20661-1subject
Has Abstractpub_date
2018-02-02 00:00:00pages
2215issue
1issn
2045-2322pii
10.1038/s41598-018-20661-1journal_volume
8pub_type
杂志文章abstract：:Dietary modulation of the gut microbiota impacts human health. Here we investigated the hitherto unknown effects of resistant starch type 4 (RS4) enriched diet on gut microbiota composition and short-chain fatty acid (SCFA) concentrations in parallel with host immunometabolic functions in twenty individuals with signs...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28797
更新日期：2016-06-30 00:00:00
abstract：:While is widely acknowledged that nonmuscle myosin II (NMMII) enables stress fibers (SFs) to generate traction forces against the extracellular matrix, little is known about how specific NMMII isoforms and functional domains contribute to SF mechanics. Here we combine biophotonic and genetic approaches to address thes...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13736
更新日期：2015-09-04 00:00:00
abstract：:Geographically diverse samples from strawberry exhibiting symptoms of Strawberry Green Petal (SbGP), periwinkle plants with virescence, and blackberry, blueberry, and raspberry plants displaying yellowing and inedible fruits, were assayed for the presence of phytoplasma DNA. PCR targeting the 16S rRNA-encoding gene an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00895-1
更新日期：2017-04-19 00:00:00
abstract：:Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41868-w
更新日期：2019-04-02 00:00:00
abstract：:New small molecules that regulate the step-wise differentiation of human pluripotent stem cells into dopaminergic neurons have been identified. The steroid, guggulsterone, was found to be the most effective inducer of neural stem cells into dopaminergic neurons. These neurons are extensively characterized and shown to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01463
更新日期：2013-01-01 00:00:00
abstract：:Gibberellins (GAs) participate in controlling various aspects of basic plant growth responses. With the exception of bryophytes, GA signalling in land plants, such as lycophytes, ferns and angiosperms, is mediated via GIBBERELLIN-INSENSITIVE DWARF1 (GID1) and DELLA proteins. To explore whether this GID1-DELLA mechanis...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11859-w
更新日期：2017-11-30 00:00:00
abstract：:Human papillomavirus (HPV) is the most common sexually transmitted infection in the world and the main cause of cervical cancer. Nowadays, the virus-like particles (VLPs) based on L1 proteins have been considered as the best candidate for vaccine development against HPV infections. Two commercial HPV (Gardasil and Cer...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51679-8
更新日期：2019-10-23 00:00:00
abstract：:Current chemical markers based quality assessment methods largely fail to reflect intrinsic chemical complexity and multiple mechanisms of action of botanical drugs (BD). The development of novel quality markers is greatly needed. Here we propose bioactive chemical markers (BCM), defined as a group of chemo-markers th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02305-y
更新日期：2017-05-25 00:00:00
abstract：:Angiopoietin-1 (Ang1) and Angiopoietin-2 (Ang2) are ligands for Tie2, an endothelial-specific receptor tyrosine kinase that is an essential regulator of angiogenesis. Here we report the identification, via expression cloning, of thrombomodulin (TM) as another receptor for Ang1 and Ang2. Thrombomodulin is an endothelia...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18912-8
更新日期：2018-01-11 00:00:00
abstract：:Iron oxide nanoparticles (IONPs) are known to induce cytotoxicity in various cancer cell lines through the generation of reactive oxygen species (ROS). However, the studies on its potential to induce toxicity in normal cell lines and in vivo system are limited and ambiguity still exists. Additionally, small molecules ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43188-5
更新日期：2019-05-06 00:00:00
abstract：:A practical quantum computer must be capable of performing high fidelity quantum gates on a set of quantum bits (qubits). In the presence of noise, the realization of such gates poses daunting challenges. Geometric phases, which possess intrinsic noise-tolerant features, hold the promise for performing robust quantum ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18414
更新日期：2015-12-21 00:00:00
abstract：:Enhancers play a crucial role in gene regulation but the participation of enhancer transcripts (i.e. enhancer RNA, eRNAs) in regulatory systems remains unclear. We provide a computational analysis on eRNAs using genome-wide data across 12 mouse tissues. The expression of genes targeted by transcribing enhancer is posi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12648
更新日期：2015-07-29 00:00:00
abstract：:While TLR-activated pathways are key regulators of B cell responses in mammals, their impact on teleost B cells are scarcely addressed. Here, the potential of Atlantic salmon B cells to respond to TLR ligands was shown by demonstrating a constitutive expression of nucleic-acid sensing TLRs in magnetic sorted IgM+ cell...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21895-9
更新日期：2018-02-23 00:00:00
abstract：:Graphene oxide-silver (Ag-GO) nanocomposite has emerged as a vital antibacterial agent very recently. In this work, we report a facile one step route of Ag-GO nanocomposite formation excluding the aid of surfactants and reductants and was successfully applied to negative Escherichia Coli (E coli) to investigate antiba...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80013-w
更新日期：2021-01-08 00:00:00
abstract：:The availability of iron (Fe) can seasonally limit phytoplankton growth in the High Latitude North Atlantic (HLNA), greatly reducing the efficiency of the biological carbon pump. However, the spatial extent of seasonal iron limitation is not yet known. We present autumn nutrient and dissolved Fe measurements, combined...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37436-3
更新日期：2019-02-05 00:00:00
abstract：:Tomatoes are known to have ameliorative effects on cardiovascular disease and cancer. The nutritional value of tomatoes can be enhanced by increasing flavonoids content through genetic modification. The regulatory gene PAP1 (production of anthocyanin pigment 1) from Arabidopsis is reported to increase initial flavonoi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12355-x
更新日期：2017-09-28 00:00:00
abstract：:Using two different hydrosilylation methods, low temperature thermal and UV initiation, silicon (111) hydrogenated surfaces were functionalized in presence of an OH-terminated alkyne, a CF3-terminated alkyne and a mixed equimolar ratio of the two alkynes. XPS studies revealed that in the absence of premeditated surfac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11299
更新日期：2015-06-12 00:00:00
abstract：:An effective analysis method was developed based on a chemometric tool for the simultaneous quantification of five different post-harvest pesticides (2,4-dichlorophenoxyacetic acid (2,4-D), carbendazim, thiabendazole, iprodione, and prochloraz) in fruits and vegetables. In the modified QuEChERS (quick, easy, cheap, ef...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42489
更新日期：2017-02-22 00:00:00
abstract：:Myocardial infarction (MI) can result in sympathetic nerve loss in the infarct region. However, the contribution of hypo-innervation to electrophysiological remodeling, independent from MI-induced ischemia and fibrosis, has not been comprehensively investigated. We present a novel mouse model of regional cardiac sympa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75903-y
更新日期：2020-11-02 00:00:00
abstract：:Pressure therapy has been proved to be an effective treatment for hypertrophic scars in a clinical setting. However, evidence-based data are controversial and the precise mechanism of action of this technique remains unknown. The aim of this study was to investigate the potential molecular mechanisms of pressure thera...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29840-6
更新日期：2018-08-07 00:00:00
abstract：:Natural hazards including floods can trigger catastrophic failures in interdependent urban transport network-of-networks (NoNs). Population growth has enhanced transportation demand while urbanization and climate change have intensified urban floods. However, despite the clear need to develop actionable insights for i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66049-y
更新日期：2020-06-25 00:00:00
abstract：:Dissecting the complex molecular interplay between the host plant and invading virus improves our understanding of the mechanisms underlying viral pathogenesis. In this study, immunoprecipitation together with the mass spectrometry analysis revealed that the heat shock protein 70 (Hsp70) family homolog, Hsc70-2, was c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22778-9
更新日期：2018-03-14 00:00:00
abstract：:The controlled interaction of two high intensity beams opens new degrees of freedom for manipulating electromagnetic waves in air. The growing number of applications for laser filaments requires fine control of their formation and propagation. We demonstrate, experimentally and theoretically, that the attraction and f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10565-x
更新日期：2017-08-31 00:00:00
abstract：:Phelan-McDermid Syndrome (PMS), which is defined by a deletion within 22q13, demonstrates significant phenotypic variation. Given that six mitochondrial genes are located within 22q13, including complex I and IV genes, we hypothesize that mitochondrial complex activity abnormalities may explain phenotypic variation in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19544
更新日期：2016-01-29 00:00:00
abstract：:Micro-algae, Schizochytrium sp., is rich source of docosahexaenoic acid, DHA (66%-lipid with 27%-DHA). Eight nutritionally balanced-diets were formulated: diet 1 (control) consisted of only fish oil (FO); diets 2 and 3 had increasing amounts of algae-meal and soybean oil (SBO) at the expense of FO; diet 4 consisted of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41020-8
更新日期：2019-04-16 00:00:00
abstract：:Global change is predicted to cause shifts in species distributions and biodiversity in arctic tundra. We applied factorial warming and nutrient manipulation to a nutrient and species poor alpine/arctic heath community for seven years. Vascular plant abundance in control plots increased by 31%. There were also notable...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10197
更新日期：2015-05-07 00:00:00
abstract：:We compared commercial kits for extraction of genomic DNA from the Gram-negative bacterium Klebsiella pneumoniae for subsequent Miseq sequencing. Purification of DNA was based on matrix binding (silica or anion exchange resin) or differential precipitation (salting out), respectively. The choice of extraction kit had ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28063
更新日期：2016-06-17 00:00:00
abstract：:Long-term monitoring of host-parasite interactions is important for understanding the consequences of infection on host fitness and population dynamics. In an eight-year survey of the loggerhead sea turtle (Caretta caretta) population nesting in Cabo Verde, we determined the spatiotemporal variation of Ozobranchus mar...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75498-4
更新日期：2020-10-29 00:00:00
abstract：:Hox proteins are transcription factors and key regulators of segmental identity along the anterior posterior axis across all bilaterian animals. Despite decades of research, the mechanisms by which Hox proteins select and regulate their targets remain elusive. We have carried out whole-genome ChIP-chip experiments to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00205
更新日期：2011-01-01 00:00:00
abstract：:Nicotinamide riboside (NR) is a newly discovered nicotinamide adenine dinucleotide (NAD+) precursor vitamin. A crystal form of NR chloride termed NIAGEN is generally recognized as safe (GRAS) for use in foods and the subject of two New Dietary Ingredient Notifications for use in dietary supplements. To evaluate the ki...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-019-46120-z
更新日期：2019-07-05 00:00:00
