Abstract：
:Local translation of membrane proteins in neuronal subcellular domains like soma, dendrites and axon termini is well-documented. In this study, we isolated the electrical signaling unit of an axon by dissecting giant axons from mature squids (Dosidicus gigas). Axoplasm extracted from these axons was found to contain ribosomal RNAs, ~8000 messenger RNA species, many encoding the translation machinery, membrane proteins, translocon and signal recognition particle (SRP) subunits, endomembrane-associated proteins, and unprecedented proportions of SRP RNA (~68% identical to human homolog). While these components support endoplasmic reticulum-dependent protein synthesis, functional assessment of a newly synthesized membrane protein in axolemma of an isolated axon is technically challenging. Ion channels are ideal proteins for this purpose because their functional dynamics can be directly evaluated by applying voltage clamp across the axon membrane. We delivered in vitro transcribed RNA encoding native or Drosophila voltage-activated Shaker KV channel into excised squid giant axons. We found that total K+ currents increased in both cases; with added inactivation kinetics on those axons injected with RNA encoding the Shaker channel. These results provide unambiguous evidence that isolated axons can exhibit de novo synthesis, assembly and membrane incorporation of fully functional oligomeric membrane proteins.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Mathur C,Johnson KR,Tong BA,Miranda P,Srikumar D,Basilio D,Latorre R,Bezanilla F,Holmgren Mdoi
10.1038/s41598-018-20684-8subject
Has Abstractpub_date
2018-02-02 00:00:00pages
2207issue
1issn
2045-2322pii
10.1038/s41598-018-20684-8journal_volume
8pub_type
杂志文章abstract：:Monanchoxymycalin C (MomC) is a new marine pentacyclic guanidine alkaloid, recently isolated from marine sponge Monanchora pulchra by us. Here, anticancer activity and mechanism of action was investigated for the first time using a human prostate cancer (PCa) model. MomC was active in all PCa cell lines at low micromo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69751-z
更新日期：2020-08-06 00:00:00
abstract：:T2-weighted cardiovascular magnetic resonance (CMR) using a 3-slice approach has been shown to accurately quantify the edema-based area-at-risk (AAR) in ST-segment elevation myocardial infarction (STEMI). We aimed to compare the performance of a 3-slice approach to full left ventricular (LV) coverage for the AAR by T1...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05127-0
更新日期：2017-07-07 00:00:00
abstract：:We propose a new express method of the correlation characterization of the particles suspended in the volume of optically transparent medium. It utilizes inline digital holography technique for obtaining two images of the adjacent layers from the investigated volume with subsequent matching of the cross-correlation fu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43840
更新日期：2017-03-02 00:00:00
abstract：:Nowadays efficient down conversion for white light emission is mainly based on rare-earth doped phosphors or cadmium-containing quantum dots. Although they exhibit high luminescence efficiency, the rare-earth mining and cadmium pollution have so far led to extremely high environmental cost, which conflicts the origina...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05307
更新日期：2014-06-16 00:00:00
abstract：:FcRn mediates recycling and transcytosis of IgG and albumin in various cell types. The MHC-class-I-like protein of the FcRn heterodimer is encoded by FCGRT. Few determinants of variable FCGRT expression in humans have been identified so far. In this study, we investigated the presence of DNA methylation in regulatory ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45203-1
更新日期：2019-06-17 00:00:00
abstract：:Secreted Protein Acidic and Rich in Cysteine (SPARC)-related modular calcium-binding protein-2 (SMOC2), a secreted matricellular protein, is reported to be involved in various processes related to cancer progression such as regulating the cell cycle, angiogenesis, and invasion. However, its expression and prognostic s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61828-z
更新日期：2020-03-17 00:00:00
abstract：:The recent characterization of the gut microbiome of traditional rural and foraging societies allowed us to appreciate the essential co-adaptive role of the microbiome in complementing our physiology, opening up significant questions on how the microbiota changes that have occurred in industrialized urban populations ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32826
更新日期：2016-09-14 00:00:00
abstract：:The high theoretical capacity and low discharge potential of silicon have attracted much attention on Si-based anodes. Herein, hollow porous SiO2 nanocubes have been prepared via a two-step hard-template process and evaluated as electrode materials for lithium-ion batteries. The hollow porous SiO2 nanocubes exhibited ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01568
更新日期：2013-01-01 00:00:00
abstract：:In the study of non-equilibrium polariton condensates it is usually assumed that the dispersion relation of polaritons is parabolic in nature. We show that considering the true non-parabolic kinetic energy of polaritons leads to significant changes in the behaviour of the condensate due to the curvature of the dispers...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01113-8
更新日期：2017-05-15 00:00:00
abstract：:In this study, to fabricate stable floating photocatalytic spheres, facile alcohol solvothermal reduction was first employed to modify commercial TiO2 (P25) photocatalysts to harvest visible light and improve their performances for photodegrading phenol in seawater exciting by visible light. Floating photocatalytic sp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50457-w
更新日期：2019-09-25 00:00:00
abstract：:Light chain (AL) amyloidosis, caused by deposition of amyloidogenic immunoglobulin light chains (LCs), is the most common systemic form in industrialized countries. Still open questions, and premises for developing targeted therapies, concern the mechanisms of amyloid formation in vivo and the bases of organ targeting...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29096
更新日期：2016-07-04 00:00:00
abstract：:Telomerase is a ribonucleoprotein enzyme, which maintains genome integrity in eukaryotes and ensures continuous cellular proliferation. Telomerase holoenzyme from the thermotolerant yeast Hansenula polymorpha, in addition to the catalytic subunit (TERT) and telomerase RNA (TER), contains accessory proteins Est1 and Es...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68107-x
更新日期：2020-07-06 00:00:00
abstract：:There has been a significant increase in autism spectrum disorder (ASD) in the last decades that cannot be exclusively attributed to better diagnosis and an increase in the communication of new cases. Patients with ASD often show dysregulation of proteins associated with synaptic plasticity, notably brain-derived neur...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74239-x
更新日期：2020-10-15 00:00:00
abstract：:The successful determination of reliable protein interaction networks (PINs) in several species in the post-genomic era has hitherto facilitated the quest to understanding systems and structural properties of such networks. It is envisaged that a clearer understanding of their intrinsic topological properties would el...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07628
更新日期：2015-02-27 00:00:00
abstract：:A hematite photoanode showing a stable, record-breaking performance of 4.32 mA/cm² photoelectrochemical water oxidation current at 1.23 V vs. RHE under simulated 1-sun (100 mW/cm²) irradiation is reported. This photocurrent corresponds to ca. 34% of the maximum theoretical limit expected for hematite with a band gap o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02681
更新日期：2013-01-01 00:00:00
abstract：:Extensive computational modeling and simulations have been carried out, in the present study, to uncover the fundamental reaction pathway for butyrylcholinesterase (BChE)-catalyzed hydrolysis of ghrelin, demonstrating that the acylation process of BChE-catalyzed hydrolysis of ghrelin follows an unprecedented single-st...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22322
更新日期：2016-02-29 00:00:00
abstract：:Cell motility requires the precise coordination of cell polarization, lamellipodia formation, adhesion, and force generation. LKB1 is a multi-functional serine/threonine kinase that associates with actin at the cellular leading edge of motile cells and suppresses FAK. We sought to understand how LKB1 coordinates these...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40929
更新日期：2017-01-19 00:00:00
abstract：:We measured the interleukin-34 (IL-34) level in sera from patients with systemic lupus erythematosus (SLE) and discoid lupus erythematosus (DLE) using an enzyme-linked immunosorbent assay (ELISA). Blood tests, including assays to determine C-reactive protein (CRP), complement (C) 3, C4, immunoglobulin (Ig) A, IgG, IgM...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21859-z
更新日期：2018-02-22 00:00:00
abstract：:Many drugs commonly prescribed during pregnancy lack a fetal safety recommendation - called FDA 'category C' drugs. This study aims to classify these drugs into harmful and safe categories using knowledge gained from chemoinformatics (i.e., pharmacological similarity with drugs of known fetal effect) and empirical dat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12943-x
更新日期：2017-10-09 00:00:00
abstract：:Schiff-based complexes as a source of cancer chemotherapeutic compounds have been subjected to the variety of anticancer studies. The in-vitro analysis confirmed the CdCl2(C14H21N3O2) complex possess cytotoxicity and apoptosis induction properties in colon cancer cells, so lead to investigate the inhibitory efficiency...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12379
更新日期：2015-07-23 00:00:00
abstract：:Facial beauty and moral beauty have been suggested to be two significant forms of social aesthetics. However, it remains unknown the extent to which there are neural underpinnings of the integration of these two forms of beauty. In the present study, participants were asked to make general aesthetic judgments of facia...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38553-3
更新日期：2019-02-13 00:00:00
abstract：:Biological research requires wide-field optical imaging techniques with resolution down to the nanometer scale to study the biological process in a sub-cell or single molecular level. To meet this requirement, wide-field structured illumination method (WFSIM) has been extensively studied. The resolution of WFSIM is de...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01521-w
更新日期：2017-05-02 00:00:00
abstract：:GABA is a key regulator of adult-born dentate granule cell (abDGC) maturation so mapping the functional connectivity between abDGCs and local interneurons is required to understand their development and integration into the hippocampal circuit. We recorded from birthdated abDGCs in mice and photoactivated parvalbumin ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74385-2
更新日期：2020-10-16 00:00:00
abstract：:Objectum-sexuality (OS) is a sexual orientation which has received little attention in the academic literature. Individuals who identify as OS experience emotional, romantic and/or sexual feelings towards inanimate objects (e.g. a bridge, a statue). We tested 34 OS individuals and 88 controls, and provide the first em...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56449-0
更新日期：2019-12-27 00:00:00
abstract：:Excess nitrite (NO2-) concentrations in water supplies is considered detrimental to the environment and human health, and is associated with incidence of stomach cancer. In this work, the authors describe a nitrite detection system based on the synthesis of gold nanoparticles (AuNPs) on reduced graphene oxide (rGO) us...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14584-6
更新日期：2017-10-27 00:00:00
abstract：:The natural course of intermittent exotropia and the factors affecting its control has been unclear. We aim to report the natural course of our cohort of 117 Chinese children with intermittent exotropia and to identify baseline parameters that may have predictive value in the control deterioration of the disease. The ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27113
更新日期：2016-06-03 00:00:00
abstract：:Understanding the excited-state dynamics in perovskite photovoltaics is necessary for progress in these materials, but changes in dynamics depending on the fabrication processes used for perovskite photoactive layers remain poorly characterised. Here we report a comparative study on femtosecond transient absorption (T...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16654-1
更新日期：2017-11-28 00:00:00
abstract：:The esophageal gland duct may serve as a pathway for the spread of early esophageal squamous cell neoplasia (ESCN) to a deeper layer. Deep intraductal tumor spreading cannot be completely eradicated by ablation therapy. However, the risk factors of ductal involvement (DI) in patients with ESCNs have yet to be investig...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-020-62668-7
更新日期：2020-04-22 00:00:00
abstract：:The emergence of single-cell RNA sequencing (scRNA-seq) technologies has enabled us to measure the expression levels of thousands of genes at single-cell resolution. However, insufficient quantities of starting RNA in the individual cells cause significant dropout events, introducing a large number of zero counts in t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34688-x
更新日期：2018-11-05 00:00:00
abstract：:Although 3,5,3'-triiodothyronine (T3) is considered to be the primary bioactive thyroid hormone (TH) due to its high affinity for TH nuclear receptors (TRs), new data suggest that 3,5-diiodothyronine (T2) can also regulate transcriptional networks. To determine the functional relevance of these bioactive THs, RNA-seq ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14913-9
更新日期：2017-11-08 00:00:00
