Propranolol suppresses the proliferation and induces the apoptosis of liver cancer cells.

Abstract:

:An increasing amount of evidence indicates that the inhibition of β adrenergic signaling can result in the inhibition of tumor growth. However, the role of propranolol in liver cancer and the underlying mechanism remain to be elucidated. The present study aimed to investigate the role of propranolol in liver cancer cell lines and provide evidence for further clinical study. Propranolol was added at different concentrations to HepG2 and HepG2.2.15 liver cancer cells and HL‑7702 normal human liver cells. The proliferation of the cell lines was monitored by live‑cell imaging at a range of time intervals. Immunofluorescence using DAPI and Hoechst 33342/propidium iodide (PI) staining, Annexin V‑FITC/PI double‑staining flow cytometry, western blotting and reverse transcription‑quantitative polymerase chain reaction were used to investigate the effect of propranolol on liver cancer cell apoptosis. The proliferation of HepG2 and HepG2.2.15 cells was inhibited by 40 and 80 µmol/l propranolol. However, the proliferation of HL‑7702 cells was not affected by <160 µmol/l propranolol. Propranolol treatment decreased the expression of adrenergic receptor β‑2 to a greater extent than adrenergic receptor β‑1, and induced apoptosis in the liver cancer cells. The apoptotic rates of HepG2 and HepG2.2.15 cells increased following treatment with propranolol, while the apoptotic rate of HL‑7702 cells was not affected. Propranolol promoted poly (ADP‑ribose) polymerase cleavage and decreased the expression of full‑length caspase‑3 in liver cancer cell lines; it induced S‑phase arrest in HepG2 and HepG2.2.15 cell lines, while HL‑7702 cells were arrested at the G0/G1 phase of the cell cycle. Thus, it was demonstrated that propranolol inhibited proliferation, promoted apoptosis and induced S-phase arrest in HepG2 and HepG2.2.15 cells.

journal_name

Mol Med Rep

authors

Wang F,Liu H,Wang F,Xu R,Wang P,Tang F,Zhang X,Zhu Z,Lv H,Han T

doi

10.3892/mmr.2018.8476

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

5213-5221

issue

4

eissn

1791-2997

issn

1791-3004

journal_volume

17

pub_type

杂志文章
  • Potential role of upregulated microRNA‑146b and ‑21 in renal fibrosis.

    abstract::The aim of the present study was to identify candidate microRNAs (miRNAs) involved in the progression of renal fibrosis. Dataset GSE42716 of miRNAs extracted from kidneys from mice with unilateral ureteral obstruction (UUO), and mice without UUO was downloaded from the Gene Expression Omnibus database. The Limma packa...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6929

    authors: Xin G,Zhou G,Zhang X,Wang W

    更新日期:2017-09-01 00:00:00

  • Research progress on human genes involved in the pathogenesis of glaucoma (Review).

    abstract::Glaucoma is the leading cause of irreversible blindness globally. It is known that the incidence of glaucoma is closely associated with inheritance. A large number of studies have suggested that genetic factors are involved in the occurrence and development of glaucoma, and even affect the drug sensitivity and prognos...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,评审

    doi:10.3892/mmr.2018.9071

    authors: Wang HW,Sun P,Chen Y,Jiang LP,Wu HP,Zhang W,Gao F

    更新日期:2018-07-01 00:00:00

  • MUC1 and maltose‑binding protein recombinant fusion protein combined with Bacillus Calmette‑Guerin induces MUC1‑specific and nonspecific anti‑tumor immunity in mice.

    abstract::Human mucin 1 (MUC1) is a target for immunotherapy. The major problem associated with MUC1‑based cancer vaccines is the weakness of the immunogenicity of MUC1. The present study aimed to develop an efficient cancer vaccine through generating a recombinant fusion protein consisting of MUC1 and maltose‑binding protein (...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2306

    authors: Fang F,Ma J,Ni W,Wang F,Sun X,Li Y,Li Q,Xie F,Wang J,Zhai R,Liu Z,Gao S,Tai G

    更新日期:2014-08-01 00:00:00

  • Simultaneous silencing of β-catenin and signal transducer and activator of transcription 3 synergistically induces apoptosis and inhibits cell proliferation in HepG2 liver cancer cells.

    abstract::The tumorigenesis and maintenance of a cancer cells is dependent upon the collaboration of multiple signaling pathways. Signal transducer and activator of transcription 3 (STAT3) and β-catenin are at the center of multiple cancer-associated signaling pathways; therefore, simultaneously targeting STAT3 and β-catenin ma...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3595

    authors: Wang XH,Liu MN,Huang P,Xu J,Liu AY,Chen J,Lv CQ,Xu RL

    更新日期:2015-08-01 00:00:00

  • Molecular basis of degenerative spinal disorders from a proteomic perspective (Review).

    abstract::Intervertebral disc degeneration (IDD) and ligamentum flavum hypertrophy (LFH) are major causes of degenerative spinal disorders. Comparative and proteomic analysis was used to identify differentially expressed proteins (DEPs) in IDD and LFH discs compared with normal discs. Subsequent gene ontology term enrichment an...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10812

    authors: Liu C,Yang M,Liu L,Zhang Y,Zhu Q,Huang C,Wang H,Zhang Y,Li H,Li C,Huang B,Feng C,Zhou Y

    更新日期:2020-01-01 00:00:00

  • Angiotensin II promotes differentiation of mouse c-kit-positive cardiac stem cells into pacemaker-like cells.

    abstract::Cardiac stem cells (CSCs) can differentiate into cardiac muscle‑like cells; however, it remains unknown whether CSCs may possess the ability to differentiate into pacemaker cells. The aim of the present study was to determine whether angiotensin II (Ang II) could promote the specialization of CSCs into pacemaker‑like ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3149

    authors: Xue C,Zhang J,Lv Z,Liu H,Huang C,Yang J,Wang T

    更新日期:2015-05-01 00:00:00

  • Polyaniline promotes peripheral nerve regeneration by enhancement of the brain‑derived neurotrophic factor and ciliary neurotrophic factor expression and activation of the ERK1/2/MAPK signaling pathway.

    abstract::A previous study has demonstrated a progression in the nerve regeneration by polyaniline/cellulose (PANI/RC), although the underlying mechanism was not elucidated. In the present study, regenerated nerves were investigated, using histological techniques, functional assays and western blot analysis. The triceps surae m...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7534

    authors: Fan L,Xiong Y,Fu Z,Xu D,Wang L,Chen Y,Xia H,Peng N,Ye S,Wang Y,Zhang L,Ye Q

    更新日期:2017-11-01 00:00:00

  • Functional analysis of the nasopharyngeal carcinoma primary tumor‑associated gene interaction network.

    abstract::The aim of the present study was to investigate the molecular mechanism of nasopharyngeal carcinoma (NPC) primary tumor development through the identification of key genes using bioinformatics approaches. Using the GSE53819 microarray dataset, acquired from the Gene Expression Omnibus database, differentially expresse...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4090

    authors: An F,Zhang Z,Xia M

    更新日期:2015-10-01 00:00:00

  • Effects of electroacupuncture combined with interleukin‑10 on chronic sinusitis in mice.

    abstract::Electroacupuncture (EA) has been documented as a form of therapy for chronic sinusitis (CRS). The present study aimed to assess the effects of EA combined with interleukin‑10 (IL‑10) overexpression on CRS in mice, and to investigate the associated mechanisms. A mouse model of CRS was established by the administration ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10375

    authors: Zhou L,Hong J,Wan Z,Lu X,Shao Y

    更新日期:2019-08-01 00:00:00

  • Chloride intracellular channel 1 regulates migration and invasion in gastric cancer by triggering the ROS-mediated p38 MAPK signaling pathway.

    abstract::Chloride intracellular channel 1 (CLIC1) has been demonstrated to be overexpressed in gastric cancer, and elevated CLIC1 expression levels are markedly associated with the processes of tumor cell migration and invasion. However, the regulatory mechanism and signaling pathway underlying these processes have remained to...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4459

    authors: Zhao W,Lu M,Zhang Q

    更新日期:2015-12-01 00:00:00

  • Dickkopf 2 promotes proliferation and invasion via Wnt signaling in prostate cancer.

    abstract::Wnt/β-catenin signaling dysregulation is involved in tumorigenesis. Furthermore, epigenetic modification of the Dickkopf (DKK) family (DKK1‑4) has been shown to be important in the regulation of Wnt signaling. However, the functions and mechanism of DKK2 in the development and progression of prostate cancer remain unc...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5502

    authors: Xu W,Pang K,Zhou ZG,Chen YF,Mo T,Li M,Liu CB

    更新日期:2016-09-01 00:00:00

  • Inhibitor of RAGE and glucose‑induced inflammation in bone marrow mesenchymal stem cells: Effect and mechanism of action.

    abstract::The occurrence and development of hyperglycemia‑induced inflammation is associated with increased expression of receptor for advanced glycation end products (RAGE) and inflammatory factors, including IL‑1β, TNF‑α and IL‑6. Previous studies have reported that the nucleotide‑binding oligomerization domain‑like receptor ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11422

    authors: Jiang M,Wang X,Wang P,Peng W,Zhang B,Guo L

    更新日期:2020-10-01 00:00:00

  • COP1 is downregulated in renal cell carcinoma (RCC) and inhibits the migration of RCC ACHN cells in vitro.

    abstract::Constitutive photomorphogenic 1 (COP1) belongs to the COP‑de-etiolated (DET)‑fusca (FUS) protein family and has been demonstrated to suppress prostate adenocarcinomas and other types of tumor, such as liver and gastric cancer. The present study investigated the expression of COP1 and its downstream factor, ets variant...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5373

    authors: Ta L,Xuan C,Xing N,Zhu X

    更新日期:2016-08-01 00:00:00

  • Comparative proteomic analysis of ductal breast carcinoma demonstrates an altered expression of chaperonins and cytoskeletal proteins.

    abstract::The aim of the present study was to analyze the protein composition of ductal breast carcinoma and the surrounding normal tissue in individual patients using comparative 2D proteomics and mass spectrometry to detect candidate disease biomarkers for diagnosis and prognosis. Samples of normal and cancerous tissue obtain...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1375

    authors: Carcoforo P,Ura B,Mischiati C,Squerzanti M,Lanzara V,Cervellati C,Calza R,De Laureto PP,Frare E,Portinari M,Feriotto G,Lanzara S,Agostinelli E,Bergamini CM

    更新日期:2013-05-01 00:00:00

  • Differentiation of human amniotic epithelial cells into osteoblasts is induced by mechanical stretch via the Wnt/β‑catenin signalling pathway.

    abstract::Human amniotic epithelial cells (hAECs) have recently been recognized as a potential source of stem cells. The present study was designed to investigate the effects of mechanical stretch on the osteogenic differentiation of hAECs. As it has been previously reported that the physical environment is an important factor ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9571

    authors: Luan F,Ma K,Mao J,Yang F,Zhang M,Luan H

    更新日期:2018-12-01 00:00:00

  • Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF-β signaling pathway.

    abstract::The transforming growth factor-β (TGF-β) signaling pathway exhibits an important role in cancer invasion and metastasis. Excessive expression of TGF-β activates Smad4, leading to the upregulation of downstream metastasis-associated genes. Thus, the inhibition of the TGF-β/Smad4 signaling pathway may be a novel strateg...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2452

    authors: Chen F,Zhuang M,Peng J,Wang X,Huang T,Li S,Lin M,Lin H,Xu Y,Li J,Chen Z,Huang Y

    更新日期:2014-10-01 00:00:00

  • Hair growth is promoted by BeauTop via expression of EGF and FGF‑7.

    abstract::Minoxidil and finasteride have been approved to treat hair loss by the Food and Drug Administration. However, the further elucidation of treatments for hair loss, including those using Chinese herbal medicine, remains important clinically. BeauTop (BT) is a health food supplement which contains Ginseng radix, Astragal...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8917

    authors: Lee CY,Yang CY,Lin CC,Yu MC,Sheu SJ,Kuan YH

    更新日期:2018-06-01 00:00:00

  • Recessive optic atrophy, sensorimotor neuropathy and cataract associated with novel compound heterozygous mutations in OPA1.

    abstract::Mutations in the optic atrophy 1 gene (OPA1) are associated with autosomal dominant optic atrophy and 20% of patients demonstrate extra-ocular manifestations. In addition to these autosomal dominant cases, only a few syndromic cases have been reported thus far with compound heterozygous OPA1 mutations, suggestive of e...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,评审

    doi:10.3892/mmr.2016.5209

    authors: Lee J,Jung SC,Hong YB,Yoo JH,Koo H,Lee JH,Hong HD,Kim SB,Chung KW,Choi BO

    更新日期:2016-07-01 00:00:00

  • Astragalus polysaccharide ameliorates H2O2-induced human umbilical vein endothelial cell injury.

    abstract::Endothelial dysfunction caused by reactive oxygen species (ROS) has been implicated in numerous cardiovascular diseases. Astragalus polysaccharide (APS), an important bioactive component extracted from the Chinese herb Astragalus membranaceus, has been widely used for the treatment of cardiovascular disease. The prese...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6515

    authors: Han R,Tang F,Lu M,Xu C,Hu J,Mei M,Wang H

    更新日期:2017-06-01 00:00:00

  • Decreased soluble RAGE in neutrophilic asthma is correlated with disease severity and RAGE G82S variants.

    abstract::The advanced glycosylation end product-specific receptor (RAGE) has been demonstrated to be an important mediator of asthma pathogenesis. The soluble isoform of RAGE (sRAGE) acts as a 'decoy' to sequester RAGE ligands, and thus prevents their binding to the receptor. A number of reports have linked deficiency of sRAGE...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.8302

    authors: Lyu Y,Zhao H,Ye Y,Liu L,Zhu S,Xia Y,Zou F,Cai S

    更新日期:2018-03-01 00:00:00

  • Rapamycin and ZSTK474 can have differential effects at different post‑infection time‑points regarding CVB3 replication and CVB3‑induced autophagy.

    abstract::Coxsackievirus B3 (CVB3) infection has been shown to stimulate autophagy. We have demonstrated that the inhibition of phosphoinositide 3‑kinase (PI3K)/protein kinase B/mammalian target of rapamycin complex (mTORC) signaling pathway could affect the autophagic reaction induced by CVB3 infection in our previous study. H...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9037

    authors: Chang H,Tian L,Chen J,Tang A,Li C,Li Z,Yang Z

    更新日期:2018-07-01 00:00:00

  • Protective effect of the herbal medicine Gan‑fu‑kang against carbon tetrachloride‑induced liver fibrosis in rats.

    abstract::The aim of the present study was to investigate the effect of a herbal medicine formula, Gan-fu-kang (GFK), on the treatment of liver fibrosis in rats and the mechanisms via which it exerts its effect. Liver fibrosis was induced in rats by subcutaneous injection of carbon tetrachloride (CCl4) at 0.5 mg/kg body weight,...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1587

    authors: Zhang C,Wang Y,Chen H,Yang G,Wang S,Jiang M,Cong L,Yuan L,Li H,Jia Y

    更新日期:2013-09-01 00:00:00

  • MicroRNA-19a mediates gastric carcinoma cell proliferation through the activation of nuclear factor-κB.

    abstract::In gastric carcinoma, the nuclear factor‑κB (NF‑κB) signaling pathway is highly active, and the constitutive activation of NF‑κB prompts malignant cell proliferation. MicroRNAs are considered to be important mediators in the regulation of the NF‑κB signaling pathway. The present study predominantly focussed on the eff...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4151

    authors: Yang F,Wang H,Jiang Z,Hu A,Chu L,Sun Y,Han J

    更新日期:2015-10-01 00:00:00

  • Gangliosides and CD82 inhibit the motility of colon cancer by downregulating the phosphorylation of EGFR at different tyrosine sites and signaling pathways.

    abstract::Previous studies have shown that (GM3), a ganglioside, suppresses hepatoma cell motility and migration by inhibiting phosphorylation of EGFR and the activity of the PI3K/AKT signaling pathway. Therefore, the aim of the present study was to investigate whether the combined treatment of CD82 with gangliosides can exert ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11467

    authors: Huang X,Li Y,He X,Chen Y,Wei W,Yang X,Ma K

    更新日期:2020-11-01 00:00:00

  • Implantation of bone mesenchymal stem cells overexpressing miRNA‑705 mitigated ischemic brain injury.

    abstract::Ischemic brain damage remains the major cause of death and disability worldwide. Bone mesenchymal stem cell (BMSC) transplantation has been identified to serve important roles in cerebral infarction due to its multi‑directional differentiation and proliferative ability. However, the function of miR‑705 combined with B...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7626

    authors: Ji M,Wang W,Li S,Hu W

    更新日期:2017-12-01 00:00:00

  • Proteins S100A8 and S100A9 are potential biomarkers for renal cell carcinoma in the early stages: results from a proteomic study integrated with bioinformatics analysis.

    abstract::In order to investigate the two members of the EF‑hand Ca2+ binding protein S100 family, S100A8 and S100A9, in renal cell carcinoma (RCC), serum samples were collected from patients with RCC, transitional cell carcinoma in the kidney, benign renal masses and normal controls. The samples were analyzed by isobaric tags ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3321

    authors: Zhang L,Jiang H,Xu G,Wen H,Gu B,Liu J,Mao S,Na R,Jing Y,Ding Q,Zhang Y

    更新日期:2015-06-01 00:00:00

  • Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG-2 hepatocellular carcinoma cells.

    abstract::The present study aimed to investigate the effects of cinobufacini injection on the proliferation and expression of topoisomerases in human HepG-2 hepatocarcinoma cells. The cells were divided into a control group and an experimental group, in which 0.105, 0.21, 0.42 mg/l cinobufacini was injected. Cell proliferation ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3552

    authors: Liu Y,Ban LY,Su X,Gao S,Liu JW,Cui XN

    更新日期:2015-07-01 00:00:00

  • Long non‑coding RNA H19 inhibition ameliorates oxygen‑glucose deprivation‑induced cell apoptosis and inflammatory cytokine expression by regulating the microRNA‑29b/SIRT1/PGC‑1α axis.

    abstract::As one of the earliest discovered long non‑coding (lnc)RNAs, lncRNA H19 imprinted maternally expressed transcript (H19) participates in regulating ischemic stroke. The present study aimed to investigate the combined roles of lncRNA H19, microRNA (miR)‑29b, silent mating‑type information regulation 2 homolog 1 (SIRT1) ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11770

    authors: Xu J,Wang C,Meng F,Xu P

    更新日期:2021-02-01 00:00:00

  • Wake-promoting actions of median nerve stimulation in TBI-induced coma: An investigation of orexin-A and orexin receptor 1 in the hypothalamic region.

    abstract::A coma is a serious complication, which can occur following traumatic brain injury (TBI), for which no effective treatment has been established. Previous studies have suggested that neural electrical stimulation, including median nerve stimulation (MNS), may be an effective method for treating patients in a coma, and ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3898

    authors: Zhong YJ,Feng Z,Wang L,Wei TQ

    更新日期:2015-09-01 00:00:00

  • Roles of microRNA-146a and microRNA-181b in regulating the secretion of tumor necrosis factor-α and interleukin-1β in silicon dioxide-induced NR8383 rat macrophages.

    abstract::Despite increasing evidence to suggest that microRNA (miR)-146a and miR-181b are involved in the regulation of immune responses and tumor progression, their roles in silicosis remain to be fully elucidated. Therefore, the present study examined the roles of miR‑146a and miR‑181b in inflammatory responses, and their ef...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4083

    authors: Zhang Y,Wang F,Lan Y,Zhou D,Ren X,Zhao L,Zhang Q

    更新日期:2015-10-01 00:00:00