Abstract:
:Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its 'melting point'. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a microcontroller based heating device implemented into a 96-well plate reader that is connected to a standard fluorescence spectrometer. We demonstrate its potential to determine the 'melting point' of soluble and membranous proteins at various buffer conditions.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Hoeser J,Gnandt E,Friedrich Tdoi
10.1038/s41598-018-19702-6subject
Has Abstractpub_date
2018-01-23 00:00:00pages
1457issue
1issn
2045-2322pii
10.1038/s41598-018-19702-6journal_volume
8pub_type
杂志文章abstract::During the late Eocene, the Earth's climate experienced several transient temperature fluctuations including the Vonhof cooling event (C16n.1n; ~35.8 Ma) hitherto known mainly from the southern oceans. Here we reconstruct sea-surface temperatures (SST) and provide δ18O and δ13C foraminiferal records for the late Eocen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41013-7
更新日期:2019-03-14 00:00:00
abstract::The Sinorhizobium meliloti (S. meliloti) strain CCNWSX0020 displayed tolerance to high levels exposures of multiple metals and growth promotion of legume plants grown in metal-contaminated soil. However, the mechanism of metal-resistant strain remains unknown. We used five P1B-ATPases deletions by designating as ∆copA...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29355
更新日期:2016-07-05 00:00:00
abstract::A tunable high-order sideband spectra generation scheme is presented by using a photonic molecule optomechanical system coupled to a waveguide beyond the perturbation regime. The system is coherently driven by a two-tone laser consisting of a continuous-wave control field and a pulsed driving field which propagates th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22920
更新日期:2016-03-10 00:00:00
abstract::Here we developed an orange light-absorbing chromoprotein named ShadowR as a novel acceptor for performing fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) measurement in living cells. ShadowR was generated by replacing hydrophobic amino acids located at the surface of the c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48604-4
更新日期:2019-08-19 00:00:00
abstract::High intracranial pressure (ICP) can impede cerebral blood flow resulting in secondary injury or death following severe stroke. Compensatory mechanisms include reduced cerebral blood and cerebrospinal fluid volumes, but these often fail to prevent raised ICP. Serendipitous observations in intracerebral hemorrhage (ICH...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78880-4
更新日期:2020-12-16 00:00:00
abstract::Novel amorphous iron hydrides (AIHs) are synthesized for the first time under ambient conditions by employing novel "quiescent reaction", without stirring for mixing solutions, during a conventional aqueous reduction-precipitation process. The kind and morphology of AIHs are dependent on the processing condition, wher...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63124-2
更新日期:2020-04-10 00:00:00
abstract::Photoacoustic tomography (PAT) offers three-dimensional (3D) structural and functional imaging of living biological tissue with label-free, optical absorption contrast. These attributes lend PAT imaging to a wide variety of applications in clinical medicine and preclinical research. Despite advances in live animal ima...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01113
更新日期:2013-01-01 00:00:00
abstract::The study aims to evaluate the effectiveness of local injection of autologous platelet rich plasma (A-PRP) as a treatment for women suffering from stress urinary incontinence (SUI). In a prospective intervention study, twenty consecutive women suffering from SUI were treated with A-PRP injection at anterior vaginal wa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80598-2
更新日期:2021-01-15 00:00:00
abstract::The present study aimed to explore the value of intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) in differentiating hepatocellular carcinoma (HCC) from intrahepatic cholangiocarcinoma (ICC). This study included 65 patients with malignant hepatic nodules (55 with HCC, 10 with ICC), and 17 control pati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64804-9
更新日期:2020-05-07 00:00:00
abstract::In this study, a simple technique was developed for the electrochemical detection of anionic analytes in weakly supported media. This was conducted by the use of electrochemical paper-based analytical devices (ePADs). A sensing platform was modified with nereistoxin and used to determine nitrite as a case study. The e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71365-4
更新日期:2020-09-03 00:00:00
abstract::Two related models for T cell signalling initiation suggest either that T cell receptor (TCR) engagement leads to its recruitment to ordered membrane domains, often referred to as lipid rafts, where signalling molecules are enriched or that ordered TCR-containing membrane nanodomains coalesce upon TCR engagement. That...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10082
更新日期:2015-05-08 00:00:00
abstract::Immunoproteasomes are known for their involvement in antigen presentation. However, their broad tissue presence and other evidence are indicative of nonimmune functions. We examined a role for immunoproteasomes in cellular responses to the endogenous and environmental carcinogen formaldehyde (FA) that binds to cytosol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00757-w
更新日期:2017-04-05 00:00:00
abstract::Effective population size reflects the history of population growth, contraction, and structuring. When the effect of structuring is negligible, the inferred trajectory of the effective population size can be informative about the key events in the history of a population. We used the IBDNe and DoRIS approaches, which...
journal_title:Scientific reports
pub_type: 历史文章,杂志文章
doi:10.1038/s41598-020-66734-y
更新日期:2020-06-16 00:00:00
abstract::Platelet activation is an important event involved in the pathophysiological processes of the coagulation system. Clinical evidence has shown that platelets undergo distinctive pathological processes during sepsis. Unfortunately, how platelets physiologically respond to inflammation or sepsis is not well understood. I...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23653
更新日期:2016-03-29 00:00:00
abstract::The determinants of Xpert MTB/RIF sensitivity, a widely used PCR test for the diagnosis of tuberculosis (TB) are poorly understood. We compared culture time-to-positivity (TTP; a surrogate of bacterial load), MTB/RIF TB-specific and internal positive control (IPC)-specific C(T) values, and clinical characteristics in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05658
更新日期:2014-07-11 00:00:00
abstract::The importance of metallurgy for social and economic development is indisputable. Although copper (Cu) was essential for the wealth of pre- and post-colonial societies in the Andes, the onset of extensive Cu metallurgy in South America is still debated. Comprehensive archaeological findings point to first sophisticate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41855
更新日期:2017-01-31 00:00:00
abstract::Accumulating of evidence suggests that activation of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) exacerbates early brain injury (EBI) following subarachnoid hemorrhage (SAH) by provoking pro-inflammatory and pro-apoptotic signaling. Myeloid differentiation primary response protein 88 (...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16124-8
更新日期:2017-11-17 00:00:00
abstract::Tissue-resident memory T (TRM) cells are characterized by their surface expression of CD69 and can be subdivided in CD103+ and CD103- TRM cells. The origin and functional characteristics of TRM cells in the renal allograft are largely unknown. To determine these features we studied TRM cells in transplant nephrectomie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42401-9
更新日期:2019-04-12 00:00:00
abstract::Recently there has not been a systematic, objective assessment of the metabolic capabilities of the human platelet. A manually curated, functionally tested, and validated biochemical reaction network of platelet metabolism, iAT-PLT-636, was reconstructed using 33 proteomic datasets and 354 literature references. The n...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03925
更新日期:2014-01-29 00:00:00
abstract::The secretion of glucagon by pancreatic alpha cells is regulated by a number of external and intrinsic factors. While the electrophysiological processes linking a lowering of glucose concentrations to an increased glucagon release are well characterized, the evidence for the identity and function of the glucose sensor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76863-z
更新日期:2020-11-19 00:00:00
abstract::The dispersion of organic N-benzyl-2-methyl-4-nitroaniline (BNA) in nematic liquid crystals (LCs) is studied. BNA doping decreases the threshold voltage of cell because of the reduced splay elastic constant and increased dielectric anisotropy of the LC mixture. When operated in the high voltage difference condition, t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71306-1
更新日期:2020-08-31 00:00:00
abstract::Our study focused on radiocaesium (137Cs) partitioning in forests, three vegetation periods after the Fukushima Daiichi nuclear power plant accident. 137Cs distribution in forest components (organic and mineral soil layers as well as tree compartments: stem, bark, needles, branches and roots) was measured for two Japa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37618
更新日期:2016-11-23 00:00:00
abstract::Transposition mutagenesis is a powerful tool to identify the function of genes, reveal essential genes and generally to unravel the genetic basis of living organisms. However, transposon-mediated mutagenesis has only been successfully applied to a limited number of archaeal species and has never been reported in Therm...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36711
更新日期:2016-11-08 00:00:00
abstract::Parasitic lice (order Phthiraptera) infest birds and mammals. The typical animal mitochondrial (mt) genome organization, which consists of a single chromosome with 37 genes, was found in chewing lice in the suborders Amblycera and Ischnocera. The sucking lice (suborder Anoplura) known, however, have fragmented mt geno...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17389
更新日期:2015-11-30 00:00:00
abstract::Environmental stimuli have the ability to generate specific representations of the rewards they predict and in so doing alter the selection and performance of reward-seeking actions. The basolateral amygdala participates in this process, but precisely how is unknown. To rectify this, we monitored, in near-real time, b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12511
更新日期:2015-07-27 00:00:00
abstract::Rodents are important reservoir hosts of many important zoonotic viruses. The family Picornaviridae contains clinically important pathogens that infect humans and animals, and increasing numbers of rodent picornaviruses have recently been associated with zoonoses. We collected 574 pharyngeal and anal swab specimens fr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34381
更新日期:2016-09-29 00:00:00
abstract::Although recent studies focused on traditional Chinese medicine (TCM) for the treatment of refractory schizophrenia have reported that it may be beneficial, there is still lack of convincing evidence and critical meta-analytic work regarding its effectiveness as an adjunctive therapy. Therefore, we performed a meta-an...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-018-24547-0
更新日期:2018-04-18 00:00:00
abstract::A relevant part of embolic strokes of undetermined source (ESUS) is assumed to be cardiogenic. As shown previously, certain biomarkers of endothelial pathology are related to atrial fibrillation (AF). In this long-term follow-up study, we aimed to investigate whether these biomarkers are associated with subsequently d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55943-9
更新日期:2019-12-19 00:00:00
abstract::Mycobacterium marinum (M. marinum) is a slowly growing nontuberculous mycobacterium. The incidence of M. marinum infections in Denmark is unknown. We conducted a retrospective nationwide study including all culture confirmed cases of M. marinum from 2004 to 2017 in Denmark. All available medical records were reviewed....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24702-7
更新日期:2018-04-30 00:00:00
abstract::We demonstrate fluorescent Fe embedded magnetic nanodiamonds by ion implantation and two-step annealing. The diamond characteristics with a highly ordered core and a graphite surface layer are maintained after the implantation process. After the two-step annealing process, a bright red fluorescence associated with nit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37820-z
更新日期:2019-02-04 00:00:00