Abstract：
:Kalopanax septemlobus is a medicinal woody species of the family Araliaceae, and the pharmaceutical properties of saponins obtained from K. septemlobus suggest that K. septemlobus has the potential to be a crude drug and dietary health supplement. In this study, we established cell suspension culture of K. septemlobus to develop a sustainable source of natura-ceuticals. Friable calli were used for establishing cell suspension culture. The maximum amount of total saponins (1.56 mg/60 ml suspension) was obtained during the 15th day of incubation, whereas the maximum capacity of saponin production was reached after day 6 (0.42 μg/mg of fresh weight). The total saponin production in the cell suspension of K. septemlobus was significantly increased by coronatine (COR) at 160% at a dose of 1 μM compared with the mock-treated control, whereas methyl jasmonate treated cells exhibited less increase in total saponin level as compared to the COR-treated cells. In addition, the elicitation of COR strongly induced the expression of beta-amyrin synthase, thus resulting in the accumulation of oleanolic acid (2.369 ± 0.98 μg/mg of extract), a precursor for oleanane-type triterpene saponins. These results indicate that COR is an efficient elicitor for inducing phytochemicals in cell suspension culture and that it provides the possibility for producing saponins of K. septemlobus using cell suspension culture.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Lee JK,Eom SH,Hyun TKdoi
10.1007/s13205-018-1090-9subject
Has Abstractpub_date
2018-01-01 00:00:00pages
59issue
1eissn
2190-572Xissn
2190-5738pii
1090journal_volume
8pub_type
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