Abstract:
:MicroRNAs (miRNAs) have an important role in the regulation of tumor development and metastasis. In this study, we investigated the clinical and prognostic value as well as biological function of miR-466 in colorectal cancer (CRC). Tumor and adjacent healthy tissues were obtained from 100 patients diagnosed with CRC. miR-466 expression was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). mRNA and protein levels of cyclin D1, apoptosis regulator BAX (BAX), and matrix metalloproteinase-2 (MMP-2) were analyzed by qRT-PCR and Western blot, respectively, in SW-620 CRC cells transfected with miR-466 mimics or negative control miRNA. Effects of miR-466 on SW-620 cell proliferation, cell cycle and apoptosis, and invasion were investigated using CCK-8 assay, flow cytometry and Transwell assay, respectively. miR-466 expression was significantly downregulated in tumor tissues compared to matched adjacent non-tumor tissues. Low expression of miR-466 was significantly correlated with the tumor size, Tumor Node Metastasis stage, lymph node metastasis, and distant metastasis. The overall survival of CRC patients with low miR-466 expression was significantly shorter compared to high-miR-466 expression group (log-rank test: p = 0.0103). Multivariate analysis revealed that low miR-466 expression was associated with poor prognosis in CRC patients. The ectopic expression of miR-466 suppressed cell proliferation and migration/invasion, as well as induced G0/G1 arrest and apoptosis in SW-620 cells. Moreover, the ectopic expression of miR-466 decreased the expression of cyclin D1 and MMP-2, but increased BAX expression in SW-620 cells. In conclusion, our findings demonstrated that miR-466 functions as a suppressor miRNA in CRC and may be used as a prognostic factor in these patients.
journal_name
Bosn J Basic Med Scijournal_title
Bosnian journal of basic medical sciencesauthors
Tong F,Ying Y,Pan H,Zhao W,Li H,Zhan Xdoi
10.17305/bjbms.2018.2376subject
Has Abstractpub_date
2018-08-01 00:00:00pages
252-259issue
3eissn
1512-8601issn
1840-4812journal_volume
18pub_type
杂志文章abstract::Diabetes mellitus can be looked upon as an array of diseases, all of which exhibit common symptoms. While pathogenesis of IDDM (insulin dependant diabetes mellitus) is well understood, the same is not true for diabetes mellitus type II. In the latter case, relative contribution of the two factors (insulin resistance o...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2006.3135
更新日期:2006-08-01 00:00:00
abstract::A cataract is a complex multifactorial disease that results from alterations in the cellular architecture, i.e. lens proteins. Genes associated with the development of lens include crystallin genes. Although crystallins are highly conserved proteins among vertebrates, a significant number of polymorphisms exist in hum...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2017.1745
更新日期:2017-05-20 00:00:00
abstract::The aim of the study was to ascertain presence of Helicobacter pylori in gastric carcinoma as a responsible promoter of inflammatory-regenerative changes, which lead to pathological differentiation and transformation of normal epithelial cells into intestinal type and, in progression, cause epithelial dysplasia that d...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2006.3146
更新日期:2006-08-01 00:00:00
abstract::The aim of this trial was to examine the efficacy and safety of antihypertensive fixed combination lisinopril plus hydrochlorothiazide (Lopril H, Bosnalijek dd) in the treatment of essential arterial hypertension. In our trial we included 297 patients, aged 54.65+/-9.6 years, with treated or untreated hypertension and...
journal_title:Bosnian journal of basic medical sciences
pub_type: 临床试验,杂志文章,多中心研究
doi:10.17305/bjbms.2007.3031
更新日期:2007-11-01 00:00:00
abstract::The new criteria in detection of ARVC/D produced more subtle noninvasive parameters that raised sensitivity in diagnosis. Since heart rate dynamics have prognostic significance for the progression of a disease and for mortality, the main objective was to explore its value in disclosing risk for serious arrhythmias. Ou...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2013.2419
更新日期:2013-02-01 00:00:00
abstract::Primary skin melanoma and skin cancers have been more prevalent in the previous decades and therefore have become a very significant public health problem. Dermatologists of the Skin and Venereal Diseases Clinic of the University of Sarajevo Clinics Centre initiated the first public preventive action called "Days of F...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2009.2861
更新日期:2009-02-01 00:00:00
abstract::Preeclampsia is referred to as the "disease of the theories" because of the multiple hypotheses proposed to explain is occurrence. Despite considerable research, the causes of preeclampsia remain unclear. Preeclampsia is likely to be multifactorial in origin, and recent research has focused on endothelial dysfunction ...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2009.2813
更新日期:2009-08-01 00:00:00
abstract::The blockade of peripheral nerves carries a certain risk of unwanted complications, which can follow after unintentional intraneural injection of a local anesthetic. Up till today, the research of measuring injection pressure has been based on animal models, even though the histological structure of periphery nerve is...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2006.3107
更新日期:2006-11-01 00:00:00
abstract::Numerous studies conducted on acetylsalicylic acid (ASA, aspirin) confirmed that ASA inhibits proliferation and induces apoptosis in various types of human cells. Therefore, it was of interest to examine possible effects of different concentrations of ASA on viability and proliferation of lymphocytes in the cell cultu...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2008.2919
更新日期:2008-08-01 00:00:00
abstract::The aim of this study was to analyze the importance of the peritoneal equilibration test (PET) in evaluation of the peritoneal membrane transport status in patients treated with continuous ambulatory peritoneal dialysis (CAPD). The study included 30 adult continuous ambulatory peritoneal dialysis (CAPD) patients, 16 m...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2010.2645
更新日期:2010-04-01 00:00:00
abstract::Number of hemodialysis patients each day is increasing. The quality of their lives is largely determined by the quality of hemodialysis treatment. One of the most important factors is the type of applied blood approach. The type of blood approach in the most case is artery venous fistula, permanent, temporary catheter...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2009.2776
更新日期:2009-11-01 00:00:00
abstract::Pituitary adenomas represent the third most common primary intracranial tumor in neurosurgical practice. To understand the biological behaviour of the pituitary adenomas previous studies have determined the tumor proliferation rate using monoclonal antibodies targeted against the Ki-67 antigen. The aim of this study w...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2017.1750
更新日期:2017-05-20 00:00:00
abstract::Owing to its unique nutritional and immunological characteristics, breast milk is the most important food source for infants. But, children are at greater risk for exposure to environmental toxicants from breast milk. The aim of this study was to evaluate the influence of environmental pollution on essential and toxic...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2011.2556
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Clinical research of drugs is a researching step subsequent to the preclinical studies in experimental animals. The aim of our research was to evaluate animal model of wound healing process after the burn inducement and effects of the ointment containing natural plants on the process of burn heal...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2003.3495
更新日期:2003-11-01 00:00:00
abstract::Cultural differences in body dissatisfaction, the relationship of stress to body dissatisfaction and individual and cultural body ideals were investigated. Forty-eight United States women and 48 Bosnian women completed the Body Shape Questionnaire (BSQ), the Impact of Event Scale (IES), and a Body Figure Rating Scale....
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2005.3332
更新日期:2005-02-01 00:00:00
abstract::Most of today available non-invasive procedures cannot clearly determinate between benign and malignant solitary pulmonary nodules (SPN). The purpose of the study was to assess the possibility of using 99mTc labeled hexakis-2-methoxyisobutylisonitrile (99mTc-MIBI) to differentiate benign from malignant SPN. Sixty pati...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2011.2570
更新日期:2011-08-01 00:00:00
abstract::Trefoil factor family (TFF) peptides are involved in the maintenance of epithelial integrity and epithelial restitution. Mature epithelial tissues originate from different embryonic germ layers. The objective of this research was to explore the presence and localization of TFF3 peptide in mouse embryonic epithelia and...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2017.1838
更新日期:2017-08-20 00:00:00
abstract::Previous studies suggested that oxidative stress is related to the onset and development of osteoporosis. Moreover, it was demonstrated that berberine has a protective effect against oxidative stress-induced injuries. In this study, we aimed to investigate the effect and mechanism of action of berberine on rats with i...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2017.2596
更新日期:2017-11-20 00:00:00
abstract::Most acute promyelocytic leukemia (APL) are characterized by reciprocal translocations t(15;17)(q22;21), which results in the fusion of PML gene at 15q22 with RARα gene at 17q21. However, several complex variant translocations also have been reported. Here we report a 62-year-old man with typical morphology and clinic...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章,评审
doi:10.17305/bjbms.2020.5106
更新日期:2020-10-09 00:00:00
abstract::Although a link between dietary changes, caries, and dental development has been observed, the literature provides little insight about this relationship. The aim of our study was to investigate the association between dental caries and dental development in a clinical sample of Albanian children and adolescents. In t...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2018.2841
更新日期:2018-05-20 00:00:00
abstract::One of the most frequent hereditary causes of thrombophilia is, without a doubt, resistance to Activated Protein C (APC-resistance), which is a consequence of point mutation in gene coding for coagulation Factor V (Factor V Leiden) in 90-95% of cases. The aim of this paper was to determine prevalence of APC-resistance...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2009.2789
更新日期:2009-11-01 00:00:00
abstract::The aim of our study was to examine NPHS1, NPHS2, WT1 and LAMB2 mutations, previously reported in two thirds of patients with nephrotic syndrome with onset before the age of one year old. Genomic DNA samples from Polish children (n=33) with Steroid-Resistant Nephrotic Syndrome (SRNS) due to focal segmental glomerulosc...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2014.2270
更新日期:2014-05-01 00:00:00
abstract::Aim of this study was to evaluate the biotransformation of simple phenols after ingestion of edible fruits and mixed food. It was analyzed hippuric acid in urine as biomarker of conjugation in the liver cells of glycine with aromatic phenolic acids such benzoic and salicylic acid from ingested food. Measurement of hip...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2008.2994
更新日期:2008-02-01 00:00:00
abstract::Kidney transplantation (Ktx) is generally performed during end stage renal disease due to a loss of the kidneys' ability to filter wastes from the circulatory system. Acute graft-versus-host disease (GVHD) after Ktx is a life-threatening complication that progresses to organ failure, systemic complications, and death....
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2011.2594
更新日期:2011-05-01 00:00:00
abstract::Large scale genetic association meta-analyses showed that neurocan (NCAN) gene polymorphism rs1064395 is susceptibility locus for bipolar disorder. These studies also included patients with bipolar disorder originated from Bosnia and Herzegovina. Followed by theory of shared genetic elements between bipolar disorder a...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2012.2446
更新日期:2012-11-01 00:00:00
abstract::After lung cancer colorectal cancer (Cc) is ranked the second, as a cause of cancer-related death. The purpose of this study was to analyze the Cc cases in our material with respect to all prognostic values including histological type and grade, vascular invasion, perineural invasion, and tumor border features. There ...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2009.2851
更新日期:2009-02-01 00:00:00
abstract::The aim of this study is to compare two major urological procedures in terms of patient exposure to radiation. We evaluated 175 patients, that were subjected to retrograde ureteroscopy (URS) and extracorporeal shock waves lithotripsy (ESWL) for lumbar or pelvic ureteral lithiasis, at two urological departments. The C-...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2014.4.99
更新日期:2014-10-18 00:00:00
abstract::The current study evaluates the impact of high saturated fat feeding in rat model of experimental nephrotoxicity induced by gentamicin. Sprague-Dawley rats weighing 200 g were randomized into four groups; the first one received the standard rodents chow for 8 weeks and was treated as control, the second group (HFD)rec...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2012.2527
更新日期:2012-02-01 00:00:00
abstract::The aim of the study was to verify the presence of mutated tumor suppresser gene p53 in intestinal mucosa with histologically confirmed premalignant lesions and gastric carcinoma, and assess its prognostic value. The paper presents prospective study that included 50 patients with gastric adeno-carcinoma of intestinal ...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2007.3080
更新日期:2007-02-01 00:00:00
abstract::In this paper we present introduction and development of some new analytical methods for identification of anabolic steroids, their metabolites and certain hormones, especially determination of exogenous testosterone by means of gas chromatography-mass spectrometry. Identification of central nervous stimulants and cor...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2003.3528
更新日期:2003-07-01 00:00:00