Abstract:
:Sirtuins are a family of enzymes, which govern a number of cellular processes essential for maintaining physiological balance. SIRT6, a nuclear sirtuin, is implicated in the development of metabolic disorders. The role of SIRT6 in regulation of cardiac metabolism is unexplored. Although glucose is not the primary energy source of heart, defects in glucose oxidation have been linked to heart failure. SIRT6+/- mice hearts exhibit increased inhibitory phosphorylation of PDH subunit E1α. SIRT6 deficiency enhances FoxO1 nuclear localization that results in increased expression of PDK4. We show that SIRT6 transcriptionally regulates the expression of PDK4 by binding to its promoter. SIRT6+/- hearts show accumulation of lactate, indicating compromised mitochondrial oxidation. SIRT6 deficiency results in decreased oxygen consumption rate and concomitantly lesser ATP production. Mechanistically, SIRT6 deficiency leads to increased FoxO1-mediated transcription of PDK4. Our findings establish a novel link between SIRT6 and cardiac metabolism, suggesting a protective role of SIRT6 in maintaining cardiac homeostasis.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Khan D,Sarikhani M,Dasgupta S,Maniyadath B,Pandit AS,Mishra S,Ahamed F,Dubey A,Fathma N,Atreya HS,Kolthur-Seetharam U,Sundaresan NRdoi
10.1002/jcp.26434subject
Has Abstractpub_date
2018-07-01 00:00:00pages
5478-5489issue
7eissn
0021-9541issn
1097-4652journal_volume
233pub_type
杂志文章abstract::Expression of plasminogen activator inhibitor type-1 (PAI-1), a member of the SERPIN gene family that functions to regulate the plasmin-based pericellular proteolytic cascade, is growth state-regulated in normal rat kidney (NRK) cells (Ryan and Higgins, 1990, J. Cell. Physiol., 155:376-384; Ryan et al., 1996, Biochem....
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199701)170:1<8::AID-JCP2>3
更新日期:1997-01-01 00:00:00
abstract::Retinoic acid reduces the growth rate of mouse S91 melanoma cells in culture and increases the proportion of cells in the G1 phase of the cell cycle. Because of the integral role protein synthesis has been shown to play in growth control we studied the effect of retinoic acid on the protein synthesis machinery with a ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041130110
更新日期:1982-10-01 00:00:00
abstract::Exposure of neutrophils to a variety of agonists including soluble chemoattractant peptides and cytokines results in degranulation and activation of the oxidative burst (effector functions) that are required for bacterial killing. At present, the signaling pathways regulating these important functions are incompletely...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199707)172:1<94::AID-JCP11
更新日期:1997-07-01 00:00:00
abstract::Parathyroid hormone-related protein (PTHrP) (107-139), in contrast to the N-terminal fragment PTHrP (1-36), has been shown to interact with the vascular endothelial growth factor (VEGF) system to modulate human osteoblast differentiation. In this study, we evaluated whether this interaction might affect human osteobla...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21547
更新日期:2008-12-01 00:00:00
abstract::The present study extends our previous observation (Kasambalides and Lanks, J. Cell. Physiol., 114:93-98, 1983), that dexamethasone inhibits the alterations in heat shock protein (HSP) and glucose-regulated protein (GRP) synthesis caused by glucose deprivation. We now show that insulin, even in the presence of high ex...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041230220
更新日期:1985-05-01 00:00:00
abstract::Destructive bone diseases caused by osteolysis are increasing in incidence. They are characterized by an excessive imbalance of osteoclast formation and activation. During osteolysis, the activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways are triggered by receptor ac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27924
更新日期:2019-07-01 00:00:00
abstract::An imbalance between matrix synthesis and degradation is the hallmark of intervertebral disc degeneration while inflammatory cytokines contribute to the imbalance. Bromodomain and extra-terminal domain (BET) family is associated with the pathogenesis of inflammation, and inhibition of BRD4, a vital member of BET famil...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29508
更新日期:2020-07-01 00:00:00
abstract::Previously, we found that hypoxia can deeply affect the production of cytokines in human peripheral mononuclear cells (PBMC). Here, we demonstrated that the cycle progression of hypoxic PBMC, cultured in the presence or not of a specific T cell activator such as phytohaemagglutinin (PHA), was delayed when compared wit...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199912)181:3<448::AID-JCP8
更新日期:1999-12-01 00:00:00
abstract::Phenotypically different osteoclasts may be generated from different subsets of precursors. To what extent the formation of these osteoclasts is influenced or mediated by the inflammatory cytokine TNF-α, is unknown and was investigated in this study. The osteoclast precursors early blasts (CD31hi Ly-6C- ), myeloid bla...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26024
更新日期:2017-12-01 00:00:00
abstract::Islets of Langerhans control whole body glucose homeostasis, as they respond, releasing hormones, to changes in nutrient concentrations in the blood stream. The regulation of hormone secretion has been the focus of attention for a long time because it is related to many metabolic disorders, including diabetes mellitus...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.25176
更新日期:2016-04-01 00:00:00
abstract::We have measured endogenous and induced rates of 70-kD, 89-kD, and 110-kD heat shock proteins in highly pure G1-, S-, or G2-M phase fractions of Chinese hamster fibroblasts (CHO) separated by fluorescence-activated cell sorting (FACS). Relative rates of synthesis of all three polypeptides as measured by two-dimensiona...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041260220
更新日期:1986-02-01 00:00:00
abstract::Male breast cancer (MBC) is an uncommon malignancy. We have previously reported that the expression of the Hippo transducers TAZ/YAP and their target CTGF was associated with inferior survival in MBC patients. Preclinical evidence demonstrated that Axl is a transcriptional target of TAZ/YAP. Thus, we herein assessed A...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25745
更新日期:2017-08-01 00:00:00
abstract::Diabetic retinopathy (DR) is complication resulted from Type 2 diabetes mellitus. Accumulating evidence has proved the functions of long noncoding RNAs (lncRNAs) in the progression of DR. Recent reports exert the numerous regulatory functions of lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in various diseases...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29740
更新日期:2020-12-01 00:00:00
abstract::Cell adhesion receptors, referred to as integrins, are recognized as key regulators of cellular processes including growth and differentiation. Integrins communicate with growth factor receptors (GFRs) to control specific cellular responses to stimuli originating in the extracellular environment. In this article, we r...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.21278
更新日期:2007-12-01 00:00:00
abstract::Parkinsonism is one of the most common aging neurodegenerative disorders. This study aims to compare the therapeutic effect of stem cell versus its conditioned medium in the Parkinsonism model. Parkinsonism was induced by daily subcutaneous injection of 0.5 mg/kg of rotenone dissolved in dimethyl sulfoxide for 28 days...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29872
更新日期:2021-01-01 00:00:00
abstract::Cellular senescence is a growth-arrest program that limits cell proliferation. Low-power laser irradiation (LPLI) has been demonstrated to promote cell proliferation. However, whether LPLI can inhibit cellular senescence remains unknown. In the present study, to investigate the functional role of LPLI against skin agi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24425
更新日期:2014-01-01 00:00:00
abstract::Optical indicators of the cationic, cyanine and anionic oxonol classes were used to evaluate the plasma membrane potential of animal cells in suspension and in monolayer culture. The optical signals were calibrated by using diffusion potentials either of K+ (in the presence of valinomycin) or of H+ (in the presence of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041230306
更新日期:1985-06-01 00:00:00
abstract::The aim of this study was to identify the molecules and pathways involved in the cross-talk between meniscus and synovium that may play a critical role in osteoarthritis (OA) pathophysiology. Samples of synovium and meniscus were collected from patients with early and end-stage OA and cultured alone or cocultured. Cyt...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27766
更新日期:2019-07-01 00:00:00
abstract::Wound healing is critically affected by age, ischemia, and growth factors such as TGFbeta1. The combined effect of these factors on fibroblast migration, an essential component of wound healing, is poorly understood. To address this deficiency, we examined expression of TGFbeta receptor type I and II (TGFbetaRI and RI...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10060
更新日期:2002-02-01 00:00:00
abstract::Methylglyoxal bis-(guanylhydrazone) (mGBG) blocked the stimulation of DNA synthesis in quiescent, density-inhibited BALB/c-3T3 cells treated with platelet-derived growth factor (PDGF) and platelet-poor plasma (PPP). Competence formation produced by a transient exposure to PDGF was not effected by mGBG. In contrast, mG...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041070105
更新日期:1981-04-01 00:00:00
abstract::In higher eukaryotes there is a link between time of replication and transcription. It is generally accepted that genes that are actively transcribed are replicated in the first half of S phase while inactive genes replicate in the second half of S phase. We have recently reported that in normal human fibroblasts ther...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.21156
更新日期:2007-11-01 00:00:00
abstract::At the initial stage of cell-cell contact of epithelial cells, primordial spot-like junctions are formed at the tips of thin cellular protrusions radiating from adjacent cells, where E-cadherin and ZO-1 are precisely coconcentrated (Yonemura et al., 1995, J. Cell Sci. 108:127-142). In fully polarized epithelial cells,...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199905)179:2<115::AID-JCP1
更新日期:1999-05-01 00:00:00
abstract::The components of 45calcium (Ca) uptake were studied in saponin skinned rat caudal artery. The steady-state Ca content increased when the free Ca concentration was varied from 10(-8) to 10(-4) M but was reduced by azide when the free Ca concentration exceeded 3.1 microM. The azide sensitivity and low affinity for Ca w...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041490305
更新日期:1991-12-01 00:00:00
abstract::We recently reported that the growth of normal rat pleural mesothelial cells (RPMCs) is inhibited by conditioned media from either in vivo or in vitro transformed RPMCs. In this study we report that the growth of normal RPMCs is inhibited by epidermal growth factor (EGF). This was demonstrated by using three methods o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041440118
更新日期:1990-07-01 00:00:00
abstract::Inhibition of insulin-induced 3T3-L1 preadipocyte differentiation by rapamycin has been attributed to a blockade of the early critical clonal expansion phase of the adipogenic program. Rapamycin binds to, and inhibits, mTOR (mammalian target of rapamycin), leading to diminution of p70 S6 kinase activity and eukaryotic...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1132
更新日期:2001-10-01 00:00:00
abstract::A monoclonal antibody (mAb), 2E12, against the neural cell adhesion molecule L1 recognized the 200 kDa component of L1. The epitope of L1 reacting with mAb 2E12 was localized in its carbohydrate chain, judging from the results of experiments on glycopeptidase F treatment. The physiological effect of adding mAbL1 (2E12...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041530211
更新日期:1992-11-01 00:00:00
abstract::Gastric cancer prognoses are persistently poor due to cancer's penchant to metastasize. As a crucial regulator of signal transducer and activator of transcription (STAT3) signaling, sirtuin 1's (SIRT1) function in gastric cancer has not been well understood. Here, we report upregulated expression of SIRT1 in tissues i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28186
更新日期:2019-09-01 00:00:00
abstract::Our study examines the effect of apoptosis on prothymosin alpha, an abundant, nuclear protein intimately involved with proliferation of all mammalian cells. When HeLa cells were treated with actinomycin D, with etoposide, or with staurosporine following synchronization with hydroxyurea, they underwent apoptosis based ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(200002)182:2<256::AID-JCP1
更新日期:2000-02-01 00:00:00
abstract:OBJECTIVE:This study aimed to explore the underlying function of Gubenxiezhuo dialysis on the distribution of gut microflora uremia. METHODS:A uremia rat model was constructed, and the morphology of renal tissue was determined using the hematoxylin-eosin (H&E) staining. Moreover, the blood samples were collected and t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27845
更新日期:2019-07-01 00:00:00
abstract::alpha-Thrombin, bradykinin, and histamine are endogenous mediators that increase endothelial permeability. We examined the mechanism by which these three vasoactive mediators could alter permeability to albumin of human umbilical vein endothelial cells (HUVEC). HUVEC were grown to confluence on Transwell membranes and...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199606)167:3<562::AID-JCP2
更新日期:1996-06-01 00:00:00