Abstract:
Background:The crystallinity of cellulose is a principal factor limiting the efficient hydrolysis of biomass to fermentable sugars or direct catalytic conversion to biofuel components. We evaluated the impact of TFA-induced gelatinization of crystalline cellulose on enhancement of enzymatic digestion and catalytic conversion to biofuel substrates. Results:Low-temperature swelling of cotton linter cellulose in TFA at subzero temperatures followed by gentle heating to 55 °C dissolves the microfibril structure and forms composites of crystalline and amorphous gels upon addition of ethanol. The extent of gelatinization of crystalline cellulose was determined by reduction of birefringence in darkfield microscopy, loss of X-ray diffractability, and loss of resistance to acid hydrolysis. Upon freeze-drying, an additional degree of crystallinity returned as mostly cellulose II. Both enzymatic digestion with a commercial cellulase cocktail and maleic acid/AlCl3-catalyzed conversion to 5-hydroxymethylfurfural and levulinic acid were markedly enhanced with the low-temperature swollen cellulose. Only small improvements in rates and extent of hydrolysis and catalytic conversion were achieved upon heating to fully dissolve cellulose. Conclusions:Low-temperature swelling of cellulose in TFA substantially reduces recalcitrance of crystalline cellulose to both enzymatic digestion and catalytic conversion. In a closed system to prevent loss of fluorohydrocarbons, the relative ease of recovery and regeneration of TFA by distillation makes it a potentially useful agent in large-scale deconstruction of biomass, not only for enzymatic depolymerization but also for enhancing rates of catalytic conversion to biofuel components and useful bio-products.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Shiga TM,Xiao W,Yang H,Zhang X,Olek AT,Donohoe BS,Liu J,Makowski L,Hou T,McCann MC,Carpita NC,Mosier NSdoi
10.1186/s13068-017-0999-2subject
Has Abstractpub_date
2017-12-27 00:00:00pages
310issn
1754-6834pii
999journal_volume
10pub_type
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-30
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-015-0367-z
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-88
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journal_title:Biotechnology for biofuels
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doi:10.1186/s13068-016-0530-1
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-6-134
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章,评审
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2018-08-02 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2016-11-09 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2020-05-18 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-6-105
更新日期:2013-07-24 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-49
更新日期:2012-07-19 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-3-16
更新日期:2010-07-02 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-017-0945-3
更新日期:2017-11-09 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0488-z
更新日期:2016-03-29 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2017-01-19 00:00:00