Tailoring tobacco hairy root metabolism for the production of stilbenes.

Abstract:

:Tobacco hairy root (HR) cultures, which have been widely used for the heterologous production of target compounds, have an innate capacity to bioconvert exogenous t-resveratrol (t-R) into t-piceatannol (t-Pn) and t-pterostilbene (t-Pt). We established genetically engineered HR carrying the gene encoding stilbene synthase (STS) from Vitis vinifera and/or the transcription factor (TF) AtMYB12 from Arabidopsis thaliana, in order to generate a holistic response in the phenylpropanoid pathway and coordinate the up-regulation of multiple metabolic steps. Additionally, an artificial microRNA for chalcone synthase (amiRNA CHS) was utilized to arrest the normal flux through the endogenous chalcone synthase (CHS) enzyme, which would otherwise compete for precursors with the STS enzyme imported for the flux deviation. The transgenic HR were able to biosynthesize the target stilbenes, achieving a production of 40 μg L-1 of t-R, which was partially metabolized into t-Pn and t-Pt (up to 2.2 μg L-1 and 86.4 μg L-1, respectively), as well as its glucoside piceid (up to 339.7 μg L-1). Major metabolic perturbations were caused by the TF AtMYB12, affecting both primary and secondary metabolism, which confirms the complexity of biotechnological systems based on seed plant in vitro cultures for the heterologous production of high-value molecules.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Hidalgo D,Georgiev M,Marchev A,Bru-Martínez R,Cusido RM,Corchete P,Palazon J

doi

10.1038/s41598-017-18330-w

subject

Has Abstract

pub_date

2017-12-21 00:00:00

pages

17976

issue

1

issn

2045-2322

pii

10.1038/s41598-017-18330-w

journal_volume

7

pub_type

杂志文章
  • Biodiversity breakpoints along stress gradients in estuaries and associated shifts in ecosystem interactions.

    abstract::Denitrification in coastal sediments can provide resilience to eutrophication in estuarine ecosystems, but this key ecosystem function is impacted directly and indirectly by increasing stressors. The erosion and loading of fine sediments from land, resulting in sedimentation and elevated sediment muddiness, presents a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-54192-0

    authors: Douglas EJ,Lohrer AM,Pilditch CA

    更新日期:2019-11-26 00:00:00

  • Haptoglobin polymorphism and prostate cancer mortality.

    abstract::Prostate cancer is a common malignancy in men worldwide and it is known that oxidative stress is a risk factor for cancer development. A common functional haptoglobin (Hp) polymorphism, originating from a duplication of a gene segment spanning over two exons, results in three distinct phenotypes with different anti-ox...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-69333-z

    authors: Kaiser M,Thurner EM,Mangge H,Herrmann M,Semeraro MD,Renner W,Langsenlehner T

    更新日期:2020-08-04 00:00:00

  • Translational control of enzyme scavenger expression with toxin-induced micro RNA switches.

    abstract::Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic network to implement detoxificati...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-81679-6

    authors: Pollak NM,Cooper-White JJ,Macdonald J

    更新日期:2021-01-28 00:00:00

  • Analysis of Optic Chiasmal Compression Caused by Brain Tumors Using Optical Coherence Tomography Angiography.

    abstract::We have quantitatively evaluated the macular and peripapillary microvascular changes in eyes with chiasmal compression caused by brain tumors compared with healthy control eyes using optical coherence tomography angiography (OCT-A) and correlated them with other ocular parameters. This cross-sectional study involved t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59158-1

    authors: Lee GI,Park KA,Oh SY,Kong DS

    更新日期:2020-02-07 00:00:00

  • Clinicopathologic significance of MYD88 L265P mutation in diffuse large B-cell lymphoma: a meta-analysis.

    abstract::The precise clinicopathologic significance of myeloid differentiation primary response gene (MYD88) L265P mutation in diffuse large B-cell lymphomas (DLBCLs) remains elusive. To investigate the frequency and clinicopathologic significance of the MYD88 L265P mutation in DLBCLs, we conducted a meta-analysis of 40 publis...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/s41598-017-01998-5

    authors: Lee JH,Jeong H,Choi JW,Oh H,Kim YS

    更新日期:2017-05-11 00:00:00

  • Variations in plantar pressure and balance in HIV-infected children in antiretroviral therapy.

    abstract::Balance disorders have been poorly investigated and somewhat neglected in people infected with the human immunodeficiency virus, especially in children, whose have intrinsic and extrinsic risk factors that may compromise the balance. To evaluate the foot plantar pressures and the balance in children with acquired immu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41028-0

    authors: da Silva Pontes L,Callegari B,Magno L,Moraes A,Silva BG,Manso K,Barros B,Araújo AP,Silva MC,Dias GA,Vasconcelos BH,Costa E Silva A,Libonati RM,Souza GS

    更新日期:2019-03-13 00:00:00

  • Consistent responses of the microbial community structure to organic farming along the middle and lower reaches of the Yangtze River.

    abstract::Soil microorganisms play a crucial role in the biogeochemical cycling of nutrient elements and maintaining soil health. We aimed to investigate the response of bacteria communities to organic farming over different crops (rice, tea and vegetable) along the middle and lower reaches of the Yangtze River of China. Compar...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep35046

    authors: Wang W,Wang H,Feng Y,Wang L,Xiao X,Xi Y,Luo X,Sun R,Ye X,Huang Y,Zhang Z,Cui Z

    更新日期:2016-10-11 00:00:00

  • K-core robustness in ecological and financial networks.

    abstract::In many real-world networks, the ability to withstand targeted or global attacks; extinctions; or shocks is vital to the survival of the network itself, and of dependent structures such as economies (for financial networks) or even the planet (for ecosystems). Previous attempts to characterise robustness include neste...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59959-4

    authors: Burleson-Lesser K,Morone F,Tomassone MS,Makse HA

    更新日期:2020-02-25 00:00:00

  • Simple fluorometric-based assay of antibiotic effectiveness for Acinetobacter baumannii biofilms.

    abstract::Despite strengthened antimicrobial therapy, biofilm infections of Acinetobacter baumannii are associated with poor prognosis and limited therapeutic options. Assessing antibiotics on planktonic bacteria can result in failure against biofilm infections. Currently, antibiotics to treat biofilm infections are administere...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-42353-0

    authors: Wannigama DL,Hurst C,Pearson L,Saethang T,Singkham-In U,Luk-In S,Storer RJ,Chatsuwan T

    更新日期:2019-04-19 00:00:00

  • Matrix metalloproteinases (MMPs) mediate leukocyte recruitment during the inflammatory phase of zebrafish heart regeneration.

    abstract::In zebrafish, the role of matrix metalloproteinases (MMPs) in the inflammatory phase of heart regeneration following cryoinjury remains poorly understood. Here, we demonstrated an increase in MMP enzymatic activity and elevated expression of mmp9 and mmp13 in the injured area (IA) of hearts from as early as 1 day post...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-25490-w

    authors: Xu S,Webb SE,Lau TCK,Cheng SH

    更新日期:2018-05-08 00:00:00

  • Transferred monolayer MoS2 onto GaN for heterostructure photoanode: Toward stable and efficient photoelectrochemical water splitting.

    abstract::Solar-driven photoelectrochemical water splitting (PEC-WS) using semiconductor photoelectrodes is considered a promising solution for sustainable, renewable, clean, safe and alternative energy sources such as hydrogen. Here, we report the synthesis and characterization of a novel heterostructure MoS2/GaN to be used as...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-56807-y

    authors: Hassan MA,Kim MW,Johar MA,Waseem A,Kwon MK,Ryu SW

    更新日期:2019-12-27 00:00:00

  • GABA (γ-aminobutyric acid), as a thermo-protectant, to improve the reproductive function of heat-stressed mungbean plants.

    abstract::Rising global temperatures are proving to be detrimental for the agriculture. Hence, strategies are needed to induce thermotolerance in food crops to sustain the food production. GABA (γ-aminobutyric acid), a non-protein amino acid, can partially protect plants from high-temperature stress. This study hypothesises tha...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44163-w

    authors: Priya M,Sharma L,Kaur R,Bindumadhava H,Nair RM,Siddique KHM,Nayyar H

    更新日期:2019-05-24 00:00:00

  • Author Correction: CXXC5 as an unmethylated CpG dinucleotide binding protein contributes to estrogen-mediated cellular proliferation.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-66682-7

    authors: Ayaz G,Razizadeh N,Yaşar P,Kars G,Kahraman DC,Saatci Ö,Şahin Ö,Çetin-Atalay R,Muyan M

    更新日期:2020-06-16 00:00:00

  • A facile one-pot oxidation-assisted dealloying protocol to massively synthesize monolithic core-shell architectured nanoporous copper@cuprous oxide nanonetworks for photodegradation of methyl orange.

    abstract::In this report, a facile and effective one-pot oxidation-assisted dealloying protocol has been developed to massively synthesize monolithic core-shell architectured nanoporous copper@cuprous oxide nanonetworks (C-S NPC@Cu2O NNs) by chemical dealloying of melt-spun Al 37 at.% Cu alloy in an oxygen-rich alkaline solutio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep36084

    authors: Liu W,Chen L,Dong X,Yan J,Li N,Shi S,Zhang S

    更新日期:2016-11-10 00:00:00

  • Nickel clusters embedded in carbon nanotubes as high performance magnets.

    abstract::Ensembles of fcc nickel nanowires have been synthesized with defined mean sizes in the interior of single-wall carbon nanotubes. The method allows the intrinsic nature of single-domain magnets to emerge with large coercivity as their size becomes as small as the exchange length of nickel. By means of X-ray magnetic ci...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15033

    authors: Shiozawa H,Briones-Leon A,Domanov O,Zechner G,Sato Y,Suenaga K,Saito T,Eisterer M,Weschke E,Lang W,Peterlik H,Pichler T

    更新日期:2015-10-13 00:00:00

  • The Making of the Andrea Wave and other Rogues.

    abstract::Unexpectedly large ocean waves or 'rogues' are sometimes claimed to be the cause of damage to ships at sea and to offshore structures. While wind-driven wave models are capable of predicting the average characteristics of waves, the maximum height of rogues that may occur is yet unknown. Rogues form in the open ocean ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep44124

    authors: Donelan MA,Magnusson AK

    更新日期:2017-03-08 00:00:00

  • A Mouse Model of Autoimmune Cholangitis via Syngeneic Bile Duct Protein Immunization.

    abstract::Primary biliary cholangitis (PBC) is an autoimmune liver disease characterized by the destruction of interlobular biliary ductules, which progressively leads to cholestasis, hepatic fibrosis, cirrhosis, and eventually liver failure. Several mouse models have been used to clarify the pathogenesis of PBC and are general...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15661-6

    authors: Ma WT,Liu QZ,Yang JB,Yang YQ,Zhao ZB,Ma HD,Gershwin ME,Lian ZX

    更新日期:2017-11-10 00:00:00

  • Facile synthesis, microstructure and photophysical properties of core-shell nanostructured (SiCN)/BN nanocomposites.

    abstract::Increasing structural complexity at nanoscale can permit superior control over photophysical properties in the precursor-derived semiconductors. We demonstrate here the synthesis of silicon carbonitride (SiCN)/boron nitride (BN) nanocomposites via a polymer precursor route wherein the cobalt polyamine complexes used a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep39866

    authors: Zhang Q,Jia D,Yang Z,Cai D,Laine RM,Li Q,Zhou Y

    更新日期:2017-01-13 00:00:00

  • Cognitive and Microbiome Impacts of Experimental Ancylostoma ceylanicum Hookworm Infections in Hamsters.

    abstract::Hookworms are one of the most prevalent and important parasites, infecting ~500 million people worldwide. Hookworm disease is among the leading causes of iron-deficiency anemia in the developing world and is associated with significant growth stunting and malnutrition. In humans, hookworms appear to impair memory and ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44301-4

    authors: Pan SC,Ward DV,Yin Y,Hu Y,Elfawal MA,Clark RE,Aroian RV

    更新日期:2019-05-27 00:00:00

  • Mechanism of azithromycin inhibition of HSL synthesis in Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa is an opportunistic pathogen and a leading cause of nosocomial infections. Unfortunately, P. aeruginosa has low antibiotic susceptibility due to several chromosomally encoded antibiotic resistance genes. Hence, we carried out mechanistic studies to determine how azithromycin affects quorum sensi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep24299

    authors: Zeng J,Zhang N,Huang B,Cai R,Wu B,E S,Fang C,Chen C

    更新日期:2016-04-14 00:00:00

  • Indian Ocean Dipole in CMIP5 and CMIP6: characteristics, biases, and links to ENSO.

    abstract::Accurately representing the Indian Ocean Dipole (IOD) is crucial for reliable climate predictions and future projections. However, El Niño-Southern Oscillation (ENSO) and IOD interact, making it necessary to evaluate ENSO and IOD simultaneously. Using the historical simulation from 32 fifth phase of Coupled Model Inte...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-68268-9

    authors: McKenna S,Santoso A,Gupta AS,Taschetto AS,Cai W

    更新日期:2020-07-13 00:00:00

  • Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms.

    abstract::Our understanding of the structure and regulation of Plasmodium vivax genes is limited by our inability to grow the parasites in long-term in vitro cultures. Most P. vivax studies must therefore rely on patient samples, which typically display a low proportion of parasites and asynchronous parasites. Here, we present ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07275-9

    authors: Kim A,Popovici J,Vantaux A,Samreth R,Bin S,Kim S,Roesch C,Liang L,Davies H,Felgner P,Herrera S,Arévalo-Herrera M,Ménard D,Serre D

    更新日期:2017-08-10 00:00:00

  • Time-dependent X-ray diffraction studies on urea/hen egg white lysozyme complexes reveal structural changes that indicate onset of denaturation.

    abstract::Temporal binding of urea to lysozyme was examined using X-ray diffraction of single crystals of urea/lysozyme complexes prepared by soaking native lysozyme crystals in solutions containing 9 M urea. Four different soak times of 2, 4, 7 and 10 hours were used. The five crystal structures (including the native lysozyme)...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep32277

    authors: Raskar T,Khavnekar S,Hosur M

    更新日期:2016-08-30 00:00:00

  • The effects of different enrofloxacin dosages on clinical efficacy and resistance development in chickens experimentally infected with Salmonella Typhimurium.

    abstract::To investigate the optimal dosage which can improve clinical efficacy and minimize resistance, pharmacokinetics/pharmacodynamics model of enrofloxacin was established. Effect of enrofloxacin treatments on clearance of Salmonella in experimentally infected chickens and simultaneously resistance selection in Salmonella ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12294-7

    authors: Li J,Hao H,Cheng G,Wang X,Ahmed S,Shabbir MAB,Liu Z,Dai M,Yuan Z

    更新日期:2017-09-15 00:00:00

  • Dietary n-3 and n-6 polyunsaturated fatty acids, the FADS gene, and the risk of gastric cancer in a Korean population.

    abstract::n-3 polyunsaturated fatty acids (PUFAs) and n-6 PUFAs are reported to have immunomodulatory effects, but few studies have examined these functions. Thus, we examined whether dietary n-3 and n-6 PUFAs are associated with the risk of gastric cancer and further investigated whether fatty acid desaturases 1 and 2 (FADS1 a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21960-3

    authors: Lee S,Lee J,Choi IJ,Kim YW,Ryu KW,Kim YI,Kim J

    更新日期:2018-02-28 00:00:00

  • TGF-β regulates phosphorylation and stabilization of Sox9 protein in chondrocytes through p38 and Smad dependent mechanisms.

    abstract::Members of the TGF-β superfamily are important regulators of chondrocyte function. Sox9, a key transcriptional regulator of chondrogenesis, is required for TGF-β-mediated regulation of specific cartilage genes. TGF-β can signal through a canonical, Smad-mediated pathway or non-conical pathways, including p38. Here we ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep38616

    authors: Coricor G,Serra R

    更新日期:2016-12-08 00:00:00

  • Phosphatidylethanolamine-phosphatidylserine binding synergy of seven coagulation factors revealed using Nanodisc arrays on silicon photonic sensors.

    abstract::Blood coagulation is regulated through protein-protein and protein-lipid interactions that occur at the sub-endothelium following vascular damage. Soluble clotting proteins bind to membrane components in a phosphatidylserine (PS) dependent manner to assemble multi-protein complexes that regulate clot formation; howeve...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73647-3

    authors: Medfisch SM,Muehl EM,Morrissey JH,Bailey RC

    更新日期:2020-10-15 00:00:00

  • Recent changes in daily climate extremes in an arid mountain region, a case study in northwestern China's Qilian Mountains.

    abstract::Changes in climate extremes pose far-reaching consequences to ecological processes and hydrologic cycles in alpine ecosystems of the arid mountain regions. Therefore, regional assessments in various climates and mountain regions are needed for understanding the uncertainties of the change trends for extreme climate ev...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02345-4

    authors: Lin P,He Z,Du J,Chen L,Zhu X,Li J

    更新日期:2017-05-22 00:00:00

  • Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats' assay.

    abstract::Eucalyptus globulus is an aromatic medicinal plant which known for its 1,8-cineole main pharmacological constituent exhibits as natural analgesic agent. Eucalyptus globulus-loaded micellar nanoparticle was developed via spontaneous emulsification technique and further evaluation for its analgesic efficacy study, in vi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-50134-y

    authors: Aziz ZAA,Nasir HM,Ahmad A,Setapar SHM,Ahmad H,Noor MHM,Rafatullah M,Khatoon A,Kausar MA,Ahmad I,Khan S,Al-Shaeri M,Ashraf GM

    更新日期:2019-09-23 00:00:00

  • Mutual population-shift driven antibody-peptide binding elucidated by molecular dynamics simulations.

    abstract::Antibody based bio-molecular drugs are an exciting, new avenue of drug development as an alternative to the more traditional small chemical compounds. However, the binding mechanism and the effect on the conformational ensembles of a therapeutic antibody to its peptide or protein antigen have not yet been well studied...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-58320-z

    authors: Bekker GJ,Fukuda I,Higo J,Kamiya N

    更新日期:2020-01-29 00:00:00