Abstract:
INTRODUCTION:Serum proteins are generally glycosylated and solubilized, and are thus present as glycoproteins. The glycan structure of glycoproteins reflects cell differentiation status; glycan structures generated by diseased cells are distinguishable from those produced by healthy cells. Proteins may therefore serve as markers of tissues that secrete them. Several strategies for the identification of novel serum biomarkers using a combination of glycoscience-based technologies have been recently proposed. The selection of lectins for use as probes for identification of altered glycan structures represents a critical step. Areas covered: This review describes the identification of Wisteria floribunda agglutinin (WFA) as a probe that recognizes the altered glycan structure of glycoproteins secreted by diseased cells. WFA may be employed as a probe for several diseases, e.g., liver fibrosis, liver cirrhosis, prostate cancer, ovarian cancer, and IgA nephropathy. The advantage of employing WFA as a serum biomarker probe is that only very small amounts of WFA-positive glycoproteins are present in serum; therefore, WFA background in serum is very low. Expert commentary: Based on the findings to date, several WFA-positive serum biomarkers may be measured without pre-purification of target glycoproteins, indicating their utility as serum biomarkers in patients with various diseases.
journal_name
Expert Rev Proteomicsjournal_title
Expert review of proteomicsauthors
Narimatsu H,Sato Tdoi
10.1080/14789450.2018.1419066subject
Has Abstractpub_date
2018-02-01 00:00:00pages
183-190issue
2eissn
1478-9450issn
1744-8387journal_volume
15pub_type
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journal_title:Expert review of proteomics
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doi:10.1586/epr.12.24
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.1.2.153
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doi:10.1080/14789450.2018.1505511
更新日期:2018-08-01 00:00:00
abstract:INTRODUCTION:Chromatin remodeling complexes play important roles in the control of genome regulation in both normal and diseased states, and are therefore critical components for the regulation of epigenetic states in cells. Given the role epigenetics plays in cancer, for example, chromatin remodeling complexes are rou...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1374860
更新日期:2017-10-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2017.1339604
更新日期:2017-07-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
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