Involvement of upregulation of fibronectin in the pro‑adhesive and pro‑survival effects of glucocorticoid on melanoma cells.

Abstract:

:Glucocorticoids (GCs) are important stress hormones, which are used as a concomitant medication during malignant tumor chemotherapy. Clinical and preclinical studies have linked GCs to melanoma growth and progression. However, the effects and mechanism of action of GCs on the adhesion and survival of melanoma cells are still unknown. In the present study the effect of dexamethasone (Dex), a synthetic GC, on fibronectin (FN) expression and its roles in regulating the adhesion and survival of melanoma cells were investigated. It was revealed that Dex significantly increased the levels of intracellular and secreted FN in melanoma cell lines by increasing glucocorticoid receptor‑mediated FN protein stability. Additionally, it was demonstrated that Dex (100 nM) significantly promoted the adhesion and survival of melanoma cells. Silencing FN expression abrogated the pro‑adhesive and pro‑survival effects of Dex in melanoma cells. Extracellular FN significantly enhanced melanoma cell adhesion and survival in the presence of cisplatin, whereas partially blocking extracellular FN signaling with a CD44 antibody significantly reduced FN‑enhanced adhesion and survival. This indicated that the upregulation of FN contributed to the pro‑survival effect of Dex by enhancing cell adhesion. It was also observed that activation of the PI3K/AKT signaling pathway by extracellular FN was involved in the FN‑mediated increase in melanoma cell survival. These findings increase understanding of the possible mechanisms by which GCs regulate melanoma cell adhesion and survival.

journal_name

Mol Med Rep

authors

Huang GX,Qi MF,Li XL,Tang F,Zhu L

doi

10.3892/mmr.2017.8269

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

3380-3387

issue

2

eissn

1791-2997

issn

1791-3004

journal_volume

17

pub_type

杂志文章
  • Effect of oxidized low-density lipoprotein on the expression of the prorenin receptor in human aortic smooth muscle cells.

    abstract::The activation of the (pro)renin receptor (PRR) may be potentially active in the development of atherosclerotic plaques independent of angiotensin II. Our previous studies demonstrated that high glucose was able to induce the activation of PRR. The present study was designed to determine the function of oxidized low‑d...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3254

    authors: Cheng Y,Liu X,Zhang L,Zhang Y,Liu Y,Lu Y,Jiang Y

    更新日期:2015-06-01 00:00:00

  • Arginase inhibition protects against hypoxia‑induced pulmonary arterial hypertension.

    abstract::The present study aimed to determine the role of arginase (Arg) in pulmonary arterial hypertension (PAH). In vitro, human pulmonary artery smooth muscle cells (HPASMCs) were cultured under hypoxic conditions with, or without, the Arg inhibitor, S‑(2‑boronoethyl)‑l‑cysteine (BEC), for 48 h, following which the prolifer...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3994

    authors: Jiang W,Sun B,Song X,Zheng Y,Wang L,Wang T,Liu S

    更新日期:2015-09-01 00:00:00

  • Histone deacetylase inhibitor quisinostat activates caspase signaling and upregulates p53 acetylation to inhibit the proliferation of HepG2 cells.

    abstract::Histone deacetylase inhibitor (HDACi) has been a major target of anticancer agents. Quisinostat (JNJ‑26481585), a novel second‑generation HDACi, has previously demonstrated antiproliferative activity against non‑small cell lung cancer; however, the function of quisinostat in hepatocellular carcinoma (HCC) remains to b...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7355

    authors: Li F,Wang T,Wang Z,Chen X,Liu R

    更新日期:2017-11-01 00:00:00

  • Caffeine induces sustained apoptosis of human gastric cancer cells by activating the caspase‑9/caspase‑3 signalling pathway.

    abstract::Caffeine is one of the most widely consumed substances found in beverages, and has demonstrated anticancer effects in several types of cancer. The present study aimed to examine the anticancer effects of caffeine on gastric cancer (GC) cells (MGC‑803 and SGC‑7901) in vitro, and to determine whether the apoptosis‑relat...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6894

    authors: Liu H,Zhou Y,Tang L

    更新日期:2017-09-01 00:00:00

  • Dynamic features of liver fibrogenesis and fibrosis resolution in the absence of matrix metalloproteinase‑9.

    abstract::The two‑edged effect of matrix metalloproteinase‑9 (MMP9) makes it difficult to understand its role in liver fibrogenesis and fibrosis resolution. The present study aimed to investigate the dynamic features of liver fibrogenesis and fibrosis resolution in the absence of MMP9. MMP9‑/‑ mice were used to induce liver fib...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10740

    authors: Wang Q,Liu X,Zhang J,Lu L,Feng M,Wang J

    更新日期:2019-12-01 00:00:00

  • Retinitis pigmentosa‑associated rhodopsin mutant T17M induces endoplasmic reticulum (ER) stress and sensitizes cells to ER stress-induced cell death.

    abstract::Retinitis pigmentosa (RP) is a group of inherited diseases that primarily affect light‑sensitive rods and cones in the retina. Rhodopsin mutations, including the T17M mutation, are associated with the autosomal dominant form of retinitis pigmentosa (ADRP) and have been linked to abnormal protein folding. However, the ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.1987

    authors: Jiang H,Xiong S,Xia X

    更新日期:2014-05-01 00:00:00

  • Growth sensitivity in the epiphyseal growth plate, liver and muscle of SD rats is significantly enhanced by treatment with a fermented soybean product (cheonggukjang) through stimulation of growth hormone secretion.

    abstract::Cheonggukjang (CKJ), a fermented soybean product, has been reported to have beneficial effects on various chronic diseases, including cardiovascular disease, cancer and immune diseases. To investigate whether CKJ induces growth sensitivity in mammals, alterations of key parameters related to their growth were analyzed...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1757

    authors: Hwang IS,Kim JE,Lee YJ,Kwak MH,Lee HG,Kim HS,Lee HS,Hwang DY

    更新日期:2014-01-01 00:00:00

  • Interleukin‑12B is upregulated by decoy receptor 3 in rheumatoid synovial fibroblasts.

    abstract::Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly af...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.4985

    authors: Fukuda K,Miura Y,Maeda T,Hayashi S,Kurosaka M

    更新日期:2016-04-01 00:00:00

  • Oscillatory potentials in electroretinogram as an early marker of visual abnormalities in vitamin A deficiency.

    abstract::Vitamin A deficiency (VAD) caused by malnutrition and certain intestinal diseases induces visual impairments, including night blindness and photoreceptor cell dysfunction as indicated by reduced a‑ and b‑waves in an electroretinogram (ERG). The effects of VAD on the inner retinal layer cells, including amacrine and ga...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2852

    authors: Kakiuchi D,Uehara T,Shiotani M,Nakano-Ito K,Suganuma A,Aoki T,Tsukidate K,Sawada K

    更新日期:2015-02-01 00:00:00

  • Astragaloside Ⅳ reduces the expression level of P-glycoprotein in multidrug-resistant human hepatic cancer cell lines.

    abstract::Astragaloside is a saponin widely used in traditional Chinese medicine and has been reported to be a potent multidrug resistance (MDR) reversal agent. The present study investigated the role of astragaloside Ⅳ (ASIV) in the regulation of P-glycoprotein (P-gp, encoded by the mdr1 gene) and its effect on the reversal of...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2074

    authors: Wang PP,Xu DJ,Huang C,Wang WP,Xu WK

    更新日期:2014-06-01 00:00:00

  • Inhibitory effect of D3 dopamine receptors on neuropeptide Y‑induced migration in vascular smooth muscle cells.

    abstract::Abnormal migration of vascular smooth muscle cells (VSMCs) serves an important role in hypertension, atherosclerosis and restenosis following angioplasty, which is regulated numerous hormonal and humoral factors, including neuropeptide Y (NPY) and dopamine. Dopamine and NPY are both sympathetic neurotransmitters, and ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7271

    authors: Xia XW,Zhou YQ,Luo H,Zeng C

    更新日期:2017-10-01 00:00:00

  • YB1 protects cardiac myocytes against H2O2‑induced injury via suppression of PIAS3 mRNA and phosphorylation of STAT3.

    abstract::Oxidative stress serves important roles in cardiac injury during the process of ischemia/reperfusion (I/R). Y‑box protein 1 (YB1), a member of the highly conserved Y‑box protein family, is closely associated with inflammation and stress responses by regulating gene transcription, RNA splicing and mRNA translation. How...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10119

    authors: Wang S,He F,Li Z,Hu Y,Huangfu N,Chen X

    更新日期:2019-06-01 00:00:00

  • SH003‑induced G1 phase cell cycle arrest induces apoptosis in HeLa cervical cancer cells.

    abstract::Cervical cancer is a prevalent disease that may lead to mortality in women. In spite of the development of common therapeutic agents to treat cancer, there are several limitations of their use owing to side effects and drug resistance, which may induce cancer recurrence. The anticancer effects of the new herbal mixtur...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7597

    authors: Lee KM,Lee K,Choi YK,Choi YJ,Seo HS,Ko SG

    更新日期:2017-12-01 00:00:00

  • Elevated expression of PTCD3 correlates with tumor progression and predicts poor prognosis in patients with prostate cancer.

    abstract::Pentatricopeptide repeat domain protein 3 (PTCD3) is a mitochondrial RNA‑binding protein that serves a role in mitochondrial translation. PTCD3 was originally reported as an oncogene that is involved in breast cancer and lymphoma. However, the expression and function of PTCD3 in prostate cancer (PCa) are unknown. Ther...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9402

    authors: Huang Y,Jiang G,Liang X,Lan Z,Su Z,Wu H,Weng J,Jiang X

    更新日期:2018-10-01 00:00:00

  • B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages.

    abstract::B7-H4 is a co‑inhibitory molecule of the B7 family, which is expressed on antigen‑presenting cells (APCs) and is able to limit the T‑cell immune response. Macrophages act as professional APCs and are important for immunoregulation of the tumor microenvironment in breast cancer. In order to identify the association bet...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5510

    authors: Liu L,Li D,Chen S,Zhao R,Pang D,Li D,Fu Z

    更新日期:2016-09-01 00:00:00

  • Role of the PI3K‑AKT‑mTOR pathway in hepatitis B virus infection and replication.

    abstract::The replication of hepatitis B virus (HBV) may be modulated by a variety of cell signaling pathways, including the phosphatidylinositol 3‑kinase (PI3K)‑RAC‑α serine/threonine‑protein kinase (AKT)‑serine/threonine‑protein kinase mTOR (mTOR) pathway. The aim of the present study was to determine the regulatory effects o...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8395

    authors: Xiang K,Wang B

    更新日期:2018-03-01 00:00:00

  • MicroRNA-29b inhibits TGF-β1-induced fibrosis via regulation of the TGF-β1/Smad pathway in primary human endometrial stromal cells.

    abstract::Transforming growth factor (TGF)‑β1 has a key role in the regulation of fibrosis and organ dysfunction. During the pathogenesis and progression of vital organ fibrosis, the microRNA (miR)‑29 family is irregularly downregulated and exogenous supplementation of miR‑29b has a strong anti‑fibrotic capacity. However, wheth...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5062

    authors: Li J,Cen B,Chen S,He Y

    更新日期:2016-05-01 00:00:00

  • Oleuropein induces apoptosis via activation of caspases and suppression of phosphatidylinositol 3-kinase/protein kinase B pathway in HepG2 human hepatoma cell line.

    abstract::Oleuropein is a polyphenol, that is found in extra‑virgin olive oil. Previous studies have shown that oleuropein inhibits cell proliferation and induces apoptosis in breast cancer, colorectal cancer and thyroid cancer. The aim of the present study was to investigate the effects of oleuropein in hepatocellular carcinom...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3266

    authors: Yan CM,Chai EQ,Cai HY,Miao GY,Ma W

    更新日期:2015-06-01 00:00:00

  • Wnt2 overexpression protects against PINK1 mutant‑induced mitochondrial dysfunction and oxidative stress.

    abstract::The PTEN induced putative kinase 1 (PINK1) mutation is the second most common cause of autosomal recessive adolescent Parkinson's disease (PD). Furthermore, mitochondrial disorders and oxidative stress are important mechanisms in the pathogenesis of PD. Numerous members of the Wnt family have been found to be associat...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11066

    authors: Xia SR,Wen XY,Fan XL,Chen XR,Wei ZW,Li QH,Sun L

    更新日期:2020-06-01 00:00:00

  • Inhibition of varicella‑zoster virus replication by an ethanol extract of Lysimachia mauritiana.

    abstract::Varicella‑zoster virus (VZV), a ubiquitous human α‑herpesvirus, is the causative agent of chickenpox and shingles. In the present study, we investigated the antiviral activity of a 70% ethanol extract of Lysimachia mauritiana (LME) against VZV. LME strongly interfered with the replication of both laboratory and clinic...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6444

    authors: Bae S,Song YJ

    更新日期:2017-06-01 00:00:00

  • Oxidative stress modulates the expression of toll‑like receptor 3 during respiratory syncytial virus infection in human lung epithelial A549 cells.

    abstract::Toll‑like receptor 3 (TLR3) can react with double stranded RNA and is involved in the inflammatory response to respiratory syncytial virus (RSV) infection. Also, oxidative stress has been reported to be involved in RSV infection. However, the correlation between oxidative stress and TLR3 activation during RSV infectio...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9089

    authors: Wang MM,Lu M,Zhang CL,Wu X,Chen JX,Lv WW,Sun T,Qiu H,Huang SH

    更新日期:2018-08-01 00:00:00

  • Heat shock protein 70 induced by heat stress protects heterotopically transplanted hearts in rats.

    abstract::Heat shock protein 70 (HSP70) protects cardiac function against ischemia-reperfusion injury through gene transfection, although it is not a clinically practical and economical method. This study investigated whether heat stress-induced HSP70 protects heterotopically transplanted donor hearts. A total of 60 donor rats ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.982

    authors: Diao LW,Zhao LL,Qi F,Sun ZD,Zhang QH,Wu NS

    更新日期:2012-10-01 00:00:00

  • Aβ peptide secretion is reduced by Radix Polygalae-induced autophagy via activation of the AMPK/mTOR pathway.

    abstract::Radix Polygalae is a traditional Chinese medicine that has been used as a sedative and to improve memory for a number of years. The impact of Radix Polygalae in patients with Alzheimer's disease has been investigated. However the mechanisms underlying its effects remain unclear. In the current study, the toxicity of v...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3781

    authors: Zhao H,Wang ZC,Wang KF,Chen XY

    更新日期:2015-08-01 00:00:00

  • miR‑185 inhibits non‑small cell lung cancer cell proliferation and invasion through targeting of SOX9 and regulation of Wnt signaling.

    abstract::SRY-box 9 (SOX9) is an important transcription factor required for development, which has additionally been reported to be an independent prognostic indicator for the survival of patients with non‑small cell lung cancer (NSCLC). Accumulating evidence has indicated that dysregulation of microRNAs (miRNAs/miRs) may cont...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.8050

    authors: Lei Z,Shi H,Li W,Yu D,Shen F,Yu X,Lu D,Sun C,Liao K

    更新日期:2018-01-01 00:00:00

  • Silencing of URG11 expression inhibits the proliferation and epithelial‑mesenchymal transition in benign prostatic hyperplasia cells via the RhoA/ROCK1 pathway.

    abstract::Upregulated gene 11 (URG11) represents a gene upregulated by hepatitis B virus X protein and is involved in the biological processes of multifarious tumors. The present study aimed to investigate the protective effects and regulatory mechanisms of URG11 in benign prostatic hyperplasia (BPH). URG11, Ras homolog family ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8993

    authors: Zhang G,Zhu F,Han G,Li Z,Yu Q,Li Z,Li J

    更新日期:2018-07-01 00:00:00

  • Inhibition of p38MAPK potentiates mesenchymal stem cell therapy against myocardial infarction injury in rats.

    abstract::The present study aimed to investigate the protective effect of mesenchymal stem cell (MSC) therapy in combination with p38 mitogen‑activated protein (MAPK) inhibition against myocardial infarction (MI) injury in rats, and to elucidate the underlying mechanisms. An MI model was established by ligation of the anterior ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6973

    authors: Zhang Z,Zhou S,Mei Z,Zhang M

    更新日期:2017-09-01 00:00:00

  • Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells.

    abstract::Twenty‑three clinical Streptococcus pneumoniae (SP) strains were isolated from blood and sputum specimens from the Second Affiliated Hospital of Wenzhou Medical College in 2009. These strains and the ATCC 49619 standard strain were cultured and suspended in normal saline (at a turbidity of 1.0 McFarland). The producti...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1688

    authors: Zhang J,Hu DK,Wang DG,Liu Y,Liu CB,Yu LH,Qu Y,Luo XH,Yang JH,Yu J,Liu SC,Li XY

    更新日期:2013-11-01 00:00:00

  • Scavenging of blood glutamate for enhancing brain-to-blood glutamate efflux.

    abstract::The presence of excess glutamate in the brain interstitial fluid characterizes several acute pathological conditions of the brain, including traumatic brain injury and stroke. It has been demonstrated that it is possible to eliminate excess glutamate in the brain by decreasing blood glutamate levels and, accordingly, ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1793

    authors: Li Y,Hou X,Qi Q,Wang L,Luo L,Yang S,Zhang Y,Miao Z,Zhang Y,Wang F,Wang H,Huang W,Wang Z,Shen Y,Wang Y

    更新日期:2014-01-01 00:00:00

  • MicroRNA expression profiling of intestinal mucosa tissue predicts multiple crucial regulatory molecules and signaling pathways for gut barrier dysfunction of AIDS patients.

    abstract::Human immunodeficiency virus‑1 (HIV‑1) infection severely damages the gut‑associated lymphoid tissue (GALT), the immune system and the gut barrier, which leads to accelerating the disease progression for patients with acquired immune deficiency syndrome (AIDS). Dysregulation of microRNAs (miRNAs) may contribute to thi...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7722

    authors: Xu Y,Wang HW,Luo HY,Shu R,Liu J,Sun L,Han XF,Lin N,Wang TH,Zeng YJ,Wang KH

    更新日期:2017-12-01 00:00:00

  • Identification of therapeutic targets for Alzheimer's disease via differentially expressed gene and weighted gene co-expression network analyses.

    abstract::In order to investigate the pathogenic targets and associated biological process of Alzheimer's disease in the present study, mRNA expression profiles (GSE28146) and microRNA (miRNA) expression profiles (GSE16759) were downloaded from the Gene Expression Omnibus database. In GSE28146, eight control samples, and Alzhei...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5828

    authors: Jia Y,Nie K,Li J,Liang X,Zhang X

    更新日期:2016-11-01 00:00:00