Abstract:
:Intestinal injury is inevitable during exposure to high radiation doses and is a common side effect observed during abdominal/pelvic radiotherapy. Yet, no radiation countermeasures are available for gastrointestine (GI) injury management. The aim of this study is to determine the effects of podophyllotoxin and rutin in combination (G-003M) on ionising radiation induced GI injury. We prophylactically administered G-003M to C57BL/6J mice exposed to 9 Gy total body radiation (TBI) and assessed for morphological changes, loss in absorption, fluid retention, biochemical alterations, immunohistochemical analysis to study cPARP, caspase-3, PCNA expression, and TUNEL staining. The irradiated intestine demonstrated extensive loss in crypts and villi, disrupted mucosal lining with reduced xylose uptake and enhanced fluid level post 7-day radiation. Mice receiving G-003M before radiation showed significant protection to intestinal epithelium, better allocation of secretory goblet cells, recovery in absorption, and reduced intestinal oedema. Additionally, G-003M administration also prevented radiation induced ROS generation, lipid peroxidation (MDA levels) and maintained the intestinal glutathione pool compared to the irradiated animals. G-003M supplementation also resulted in restoration of intestinal mitochondrial membrane potential, which was otherwise depolarised by radiation treatment. Immunohistochemical analysis demonstrated decrease in c-PARP and caspase-3 expression in jejuna cross sections and upregulation of PCNA in G-003M treated crypt cells as compared to 9 Gy irradiated mice. Our findings show that G-003M augment survival of mice against lethal radiation by promoting structural and functional regeneration in intestinal tissue. This combination therefore can be effectively explored for preventing radiation induced GI toxicity.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Dutta A,Gupta ML,Verma Sdoi
10.1080/10715762.2017.1418982subject
Has Abstractpub_date
2018-01-01 00:00:00pages
103-117issue
1eissn
1071-5762issn
1029-2470journal_volume
52pub_type
杂志文章abstract::p38 MAPKs are important mediators of signal transduction that respond to a wide range of extracellular stressors such as UV radiation, osmotic shock, hypoxia, pro-inflammatory cytokines, and oxidative stress. The most abundant family member is p38α, which helps to couple cell proliferation and growth in response to ce...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2013.821200
更新日期:2013-11-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.684879
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journal_title:Free radical research
pub_type: 杂志文章,评审
doi:
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500143478
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journal_title:Free radical research
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journal_title:Free radical research
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doi:10.3109/10715762.2010.540574
更新日期:2011-04-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1315113
更新日期:2017-04-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701487682
更新日期:2007-10-01 00:00:00
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journal_title:Free radical research
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doi:10.3109/10715762.2010.515220
更新日期:2011-02-01 00:00:00
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journal_title:Free radical research
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600883254
更新日期:2006-11-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2018.1457217
更新日期:2018-05-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.669835
更新日期:2012-06-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1087642
更新日期:2015-01-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1380307
更新日期:2017-10-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760902870611
更新日期:2009-05-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000028316
更新日期:2002-10-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701644019
更新日期:2007-11-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2011.555479
更新日期:2011-05-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760601184819
更新日期:2007-05-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1677902
更新日期:2019-12-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
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更新日期:2015-01-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500231786
更新日期:2005-10-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400017244
更新日期:2005-02-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500401116
更新日期:2006-02-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.655244
更新日期:2012-03-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760500053651
更新日期:2005-08-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,随机对照试验
doi:10.1080/10715760802555569
更新日期:2008-11-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097782
更新日期:1997-01-01 00:00:00