Abstract:
BACKGROUND AND AIMS:CD4+CD25+Foxp3+ T-regulatory (Treg) cells control immune responses and maintain immune homeostasis. However, under inflammatory conditions, Treg cells produce cytokines that promote inflammation. We investigated production of tumor necrosis factor (TNF) by Treg cells in patients with acute hepatitis A (AHA), and examined the characteristics of these cells and association with clinical factors. METHODS:We analyzed blood samples collected from 63 patients with AHA at the time of hospitalization (and some at later time points) and 19 healthy donors in South Korea. Liver tissues were collected from patients with fulminant AHA during liver transplantation. Peripheral blood mononuclear cells were isolated from whole blood and lymphocytes were isolated from liver tissues and analyzed by flow cytometry. Cytokine production from Treg cells (CD4+CD25+Foxp3+) was measured by immunofluorescence levels following stimulation with anti-CD3 and anti-CD28. Epigenetic stability of Treg cells was determined based on DNA methylation patterns. Phenotypes of Treg cells were analyzed by flow cytometry and an RORγt inhibitor, ML-209, was used to inhibit TNF production. Treg cell suppression assay was performed by co-culture of Treg-depleted peripheral blood mononuclear cells s and isolated Treg cells. RESULTS:A higher proportion of CD4+CD25+Foxp3+ Treg cells from patients with AHA compared with controls produced TNF upon stimulation with anti-CD3 and anti-CD28 (11.2% vs 2.8%). DNA methylation analysis confirmed the identity of the Treg cells. TNF-producing Treg cells had features of T-helper 17 cells, including up-regulation of RORγt, which was required for TNF production. The Treg cells had reduced suppressive functions compared with Treg cells from controls. The frequency of TNF-producing Treg cells in AHA patients' blood correlated with their serum level of alanine aminotransferase. CONCLUSIONS:Treg cells from patients with AHA have altered functions compared with Treg cells from healthy individuals. Treg cells from patients with AHA produce higher levels of TNF, gain features of T-helper 17 cells, and have reduced suppressive activity. The presence of these cells is associated with severe liver injury in patients with AHA.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Choi YS,Jung MK,Lee J,Choi SJ,Choi SH,Lee HW,Lee JJ,Kim HJ,Ahn SH,Lee DH,Kim W,Park SH,Huh JR,Kim HP,Park JY,Shin ECdoi
10.1053/j.gastro.2017.11.277subject
Has Abstractpub_date
2018-03-01 00:00:00pages
1047-1060issue
4eissn
0016-5085issn
1528-0012pii
S0016-5085(17)36685-4journal_volume
154pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND & AIMS:Simvastatin improves liver generation of nitric oxide and hepatic endothelial dysfunction in patients with cirrhosis, so it could be an effective therapy for portal hypertension. This randomized controlled trial evaluated the effects of continuous simvastatin administration on the hepatic venous press...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2009.01.043
更新日期:2009-05-01 00:00:00
abstract::Disappearance of symptoms, resolution of most biochemical abnormalities, and histologic improvement to mild chronic inflammation were accomplished in 69 of 123 patients (56%) with severe chronic active liver disease treated with corticosteroids for up to 6.5 yr. Remission of at least 6 mo duration was possible in 35 o...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00
abstract:BACKGROUND & AIMS:In patients with chronic hepatitis C who failed to respond to standard therapy, high-dose pegylated interferon (IFN)-α and/or ribavirin could induce a stronger antiviral response and prevent treatment failure and HCV resistance when combined with direct-acting antivirals. The influence of genetic dete...
journal_title:Gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.1053/j.gastro.2011.03.039
更新日期:2011-07-01 00:00:00
abstract::Two types of benzodiazepine receptors have been identified in the central nervous system. The aim of these experiments was to determine if ligands for these receptors alter basal water absorption by rat ileum in vivo after central administration. Specifically, the effects on net water flux of the systemic and central ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(87)91023-7
更新日期:1987-08-01 00:00:00
abstract::Fifty consecutive patients with symptomatic endoscopically proven duodenal ulcer were randomized double-blind to Mylanta II or cimetidine treatment schedules. Smoking habits were noted, but patients were not advised to alter these. Healing was determined by reendoscopy at 6 wk. Eighty percent of patients on active cim...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1981-06-01 00:00:00
abstract:BACKGROUND & AIMS:Bile secretion is regulated in part by adenosine 3',5'-cyclic monophosphate (cAMP) and cytosolic Ca2+ (Ca2+i). Hormone receptors that link to these second messengers are not uniformly distributed across the hepatic lobule, but both cAMP and Ca2+i cross gap junctions, so we tested whether gap junctiona...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(99)70021-1
更新日期:1999-05-01 00:00:00
abstract:BACKGROUND AND AIMS:Earlier studies have suggested that patients with hemochromatosis have poor post-transplantation survival. We aimed to compare patients with hemochromatosis to those with other causes of liver disease with regard to post-transplantation survival. METHODS:We compared the post-transplant survival of ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.05.054
更新日期:2007-08-01 00:00:00
abstract:BACKGROUND & AIMS:This longitudinal study investigated the interactions and roles of hepatitis B virus (HBV) genotypes, pre-S deletions, and core promoter and precore mutations on the progression of liver disease in hepatitis B e antigen (HBeAg)-negative patients. METHODS:A total of 141 HBeAg-negative patients without...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.09.002
更新日期:2007-11-01 00:00:00
abstract:BACKGROUND & AIMS:Amidated gastrins are acid secretagogues and growth factors. Their precursor, progastrin, is a growth factor but not a secretagogue. Cleavage of progastrin at Arg94/95 determines the expression of these two alternative patterns of biological activity. We examined the hypothesis that cleavage at Arg94/...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(98)70086-1
更新日期:1998-11-01 00:00:00
abstract:BACKGROUND & AIMS:Trinitrobenzene sulfonic acid (TNBS)-induced colitis is a T-helper 1 (Th1) T cell-mediated inflammation that is rapidly reversed by administration of anti-interleukin (IL) 12. This study sought to define the mechanism of this curative effect. METHODS:Cells and tissue from mice with TNBS colitis recei...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(99)70392-6
更新日期:1999-11-01 00:00:00
abstract::Ethanol (10 or 15% in the drinking water) was found to increase the amount of collagen in the livers of rats receiving a cirrhogenic diet. This effect of ethanol was most striking in the animals receiving 10% ethanol. Ethanol lowered the amount of labeled proline incorporated into hydroxyproline and, therefore, into c...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::The role of capsaicin-sensitive afferent neurons in acid-induced HCO3- secretion was investigated in the duodenum of anesthetized rats. The proximal duodenum was perfused with saline (pH 4.5), the pH of perfusate and the transmucosal potential differences were continuously monitored, and HCO3- output was determined by...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(91)90721-v
更新日期:1991-10-01 00:00:00
abstract::Three analogues of the carboxyl-terminal tricosapeptide of secretin (S5-27), one with glutamine replacing glutamic acid in position 9 (9-Gln-S5-27), a second with asparagine substituted for aspartic acid in position 15 (15-Asn-S5-27), and a third with both replacements (9-Gln-15-Asn-S5-27) were tested for their abilit...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1976-12-01 00:00:00
abstract::We have examined the receptor binding of radio-iodinated murine epidermal growth factor and the effects of epidermal growth factor on both amylase release and incorporation of [3H]leucine into protein in isolated adult mouse pancreatic acini in primary culture. Binding of radioiodinated murine epidermal growth factor ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::The purpose of these experiments was to determine the contribution of gastrin to the acid secretory response to eating in healthy human subjects. To simulate the gastric and intestinal phases of eating, a meal was homogenized and then infused into the stomach through a nasogastric tube. At the same time, the cephalic ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(87)90968-1
更新日期:1987-04-01 00:00:00
abstract:BACKGROUND & AIMS:T helper (Th) 17 cells that express the retinoid-related orphan receptor (ROR) γt contribute to the development of colitis in mice, yet are found in normal and inflamed intestine. We investigated their development and functions in intestines of mice. METHODS:We analyzed intestinal Th17 cells in healt...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2012.07.108
更新日期:2012-11-01 00:00:00
abstract:BACKGROUND & AIMS:One approach to the development of targeted cancer chemotherapy exploits increased uptake of the agent into neoplastic cells. In this scenario, higher concentrations of the agent in cancer cells are responsible for differential killing, whereas the low concentration in normal human cells decreases sid...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2001.24804
更新日期:2001-06-01 00:00:00
abstract:BACKGROUND & AIMS:Early embryogenesis involves cell fate decisions that define the body axes and establish pools of progenitor cells. Development does not stop once lineages are specified; cells continue to undergo specific maturation events, and changes in gene expression patterns lead to their unique physiological fu...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2012.04.011
更新日期:2012-08-01 00:00:00
abstract:BACKGROUND & AIMS:Quantitative neurophysiological studies have identified the presence of at least 2 spinal neuronal populations (abrupt and sustained) that are excited by the noxious visceral stimulus colorectal distention. This study examined the effects of the kappa opioid receptor agonists fedotozine and U50488H on...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1999.0029900388
更新日期:1999-08-01 00:00:00
abstract::Developmental aspects of the intestinal transport of biotin were examined in suckling (16 day old) and weanling (24 day old) rats using the everted sac technique. The results were compared with those of adult rats previously reported by us using the same intestinal preparation. Transport of biotin was linear for 20 mi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(88)90611-7
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND & AIMS:It is a challenge to deliver nucleic acids to gastrointestinal (GI) tissues due to their size and need for intracellular delivery. They are also extremely susceptible to degradation by nucleases, which are ubiquitous in the GI tract. We investigated whether ultrasound, which can permeabilize tissue th...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2017.01.002
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND & AIMS:Treatment options are limited for patients with hepatitis C (HCV) infection with treatment failure after sofosbuvir plus an NS5A inhibitor. There are some data for the efficacy of glecaprevir/pibrentasvir (G/P) in these patients. We performed a randomized trial of the safety and efficacy of 12 and 16 ...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2019.08.008
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND & AIMS:Idiopathic inflammatory bowel disease (IBD) is associated with bone loss in more than 30% of cases. Nevertheless, the pathogenesis of the bone loss is uncertain. The aim of this study was to investigate the bone loss and underlying mechanisms using an animal model of IBD. METHODS:Severe colitis was i...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1996.v111.pm8898640
更新日期:1996-11-01 00:00:00
abstract::Cholinergic innervation in the aganglionic bowel of the piebald lethal mouse model of Hirschsprung's disease was investigated by analysis of muscarinic acetylcholine receptors before and after administration of hexamethonium. After hexamethonium administration in the normal rectum, the maximum specific binding (Bmax) ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90008-3
更新日期:1985-06-01 00:00:00
abstract:BACKGROUND & AIMS:Colonic crypt elongation occurs during both chronic colitis and in the recovery phase of acute colitis. The impact of these alterations on epithelial cell functions is not fully defined. METHODS:DNA microarray analysis of freshly isolated colonic epithelial cells (CECs) from acute and chronic colitis...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(03)00665-6
更新日期:2003-07-01 00:00:00
abstract:BACKGROUND & AIMS:Crohn's disease (CD) usually recurs after intestinal resection; postoperative endoscopic monitoring and tailored treatment can reduce the chance of recurrence. We investigated whether monitoring levels of fecal calprotectin (FC) can substitute for endoscopic analysis of the mucosa. METHODS:We analyze...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2015.01.026
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND & AIMS:Patients with irritable bowel syndrome with diarrhea (IBS-D) have increased mucosal serotonin (5-hydroxytryptamine [5-HT]) availability, possibly because immune activation reduces activity of the 5-HT transporter (SERT). We investigated the relationship between mucosal and platelet SERT and immune act...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2011.01.052
更新日期:2011-05-01 00:00:00
abstract:BACKGROUND & AIMS:We followed patients with ongoing hepatitis C virus (HCV) exposure following control of an initial HCV infection to determine whether primary control conferred protection against future persistent infections. METHODS:Twenty-two active injection drug users (IDU) who had cleared a primary hepatitis C v...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.09.017
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND AND AIMS:We set out to test the hypothesis that irritable bowel syndrome (IBS) is characterized by an augmented cellular immune response with enhanced production of proinflammatory cytokines. We further aimed to explore whether symptoms and psychiatric comorbidity in IBS are linked to the release of proinfla...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.01.046
更新日期:2007-03-01 00:00:00
abstract::A prospective endoscopic and histologic study of terminal ileum and colorectum in 211 patients with seronegative spondylarthropathy revealed macroscopic inflammatory lesions varying from erythema to superficial erosions in 30% of the patients and microscopic inflammation in 61%. Two types of inflammation were observed...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)91557-6
更新日期:1989-02-01 00:00:00