Abstract:
:Using genome-editing technologies to correct specific mutations represents a potentially transformative new approach for treating genetic disorders. Despite rapid advances in the field of genome editing, it is still unclear whether the long-standing goal of in vivo targeted transgene integration is feasible. This is primarily because current tools are inefficient. In particular, current technologies are incapable of targeted gene knock-in in non-dividing cells, the major building blocks of adult tissues. This poses a significant barrier for developing therapeutic strategies to treat a broad range of devastating genetic disorders. Recently, our group has developed a unique CRISPR/Cas9-based strategy, termed homology-independent targeted insertion (HITI), which enables targeted gene insertion in non-dividing cells, both in vitro and in vivo. This review will summarize current progress in developing this technology, and discuss the potential impact of HITI-based gene-correction therapies.
journal_name
J Hum Genetjournal_title
Journal of human geneticsauthors
Suzuki K,Izpisua Belmonte JCdoi
10.1038/s10038-017-0352-4subject
Has Abstractpub_date
2018-02-01 00:00:00pages
157-164issue
2eissn
1434-5161issn
1435-232Xpii
10.1038/s10038-017-0352-4journal_volume
63pub_type
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